MEASUREMENT OF BLOOD LEAD BY IMMUNOASSAY

Information

• Research Project
• 2155596

• ApplicationId

  2155596

• Core Project Number

  R43ES006734

• Full Project Number

  1R43ES006734-01

• Serial Number

  6734

• FOA Number

• Sub Project Id

• Project Start Date

  9/30/1994 - 30 years ago

• Project End Date

  3/29/1995 - 29 years ago

• Program Officer Name

• Budget Start Date

  9/30/1994 - 30 years ago

• Budget End Date

  3/29/1995 - 29 years ago

• Fiscal Year

  1994

• Support Year

  1

• Suffix

• Award Notice Date

  9/23/1994 - 30 years ago

Organizations

• BIONEBRASKA, INC.

The purpose of this project is to develop an immunoassay that can be used in screening programs for determination of blood lead levels. Monoclonal antibodies specific for delta-aminolevulinic acid (ALAD) and lead will be used to investigate the feasibility of their use for detection of lead bound to ALAD in an erythrocyte lysate. The system will be evaluated first with purified ALAD containing known amounts of lead. An ALAD-specific antibody will be adsorbed to a solid phase and used to capture the lead-containing ALAD. The lead-specific antibody will then be used in an ELISA to quantify the amount of lead bound to the ALAD and compare the results to those obtained by atomic absorption. After successful completion of these experiments, human blood samples containing known amounts of lead will be substituted for the ALAD in the assay. Erythrocytes will be lysed with a nonionic detergent, and the ALAD- specific antibody will be used to capture the released ALAD. The lead- specific antibody will then be added for detection by ELISA of lead bound to ALAD. The results will be correlated with the lead concentrations in the blood samples determined by anodic stripping voltametry or graphite- furnace atomic absorption.

IC Name

• Activity

  R43

• Administering IC

  ES

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  114

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  ZRG7

• Study Section Name

• Organization Name

  BIONEBRASKA, INC.

• Organization Department

• Organization DUNS

• Organization City

  LINCOLN

• Organization State

  NE

• Organization Country

  UNITED STATES

• Organization Zip Code

  68504

• Organization District

  UNITED STATES