MEMBRANE-BASED THIOL/DISULFIDE EXCHANGE

Information

  • Research Project
  • 2182527
  • ApplicationId
    2182527
  • Core Project Number
    R44GM044463
  • Full Project Number
    5R44GM044463-03
  • Serial Number
    44463
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/1/1990 - 34 years ago
  • Project End Date
    3/31/1995 - 29 years ago
  • Program Officer Name
  • Budget Start Date
    4/1/1993 - 31 years ago
  • Budget End Date
    3/31/1995 - 29 years ago
  • Fiscal Year
    1993
  • Support Year
    3
  • Suffix
  • Award Notice Date
    3/11/1993 - 31 years ago
Organizations

MEMBRANE-BASED THIOL/DISULFIDE EXCHANGE

DESCRIPTION (Adapted from applicant's abstract):Proteins are increasingly being used as pharmaceuticals, diagnostic agents, catalysts in biochemical processes, and growth promoters in mammalian-cell culture. Commercial processes involving the use of thiol-containing proteins are hampered by the lack of practical processes to manipulate the oxidation state of protein thiols. Although the oxidation state of protein thiols can be manipulated by thiol/disulfide exchange, conventional processes require adding an excess amount of expensive, potentially hazardous thiol reagent. This program is aimed at the development of a novel membrane-based thiol/disulfide-exchange process that overcomes these drawbacks. The process uses a catalytic amount of thiol reagent that is continuously regenerated using a membrane-based process. In Phase I, the investigator successfully demonstrated feasibility of the process, using it: 1) to reduce the disulfide linkage in oxidized glutathione, and 2) to reactivate essential thiols on an enzyme that had been inactivated by air oxidation. In Phase II, the investigators will:1) characterize the process, 2) demonstrate the applicability of the process to three target applications, 3) identify scale-up issues, and 4) demonstrate the technical and economic feasibility of the process to produce a target product at pilot-plant scale in preparation for Phase III commercialization.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R44
  • Administering IC
    GM
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    BEND RESEARCH, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    BEND
  • Organization State
    OR
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    977018583
  • Organization District
    UNITED STATES