Claims
- 1. An improved fermentation process for producing pseudomonic acid, comprising the steps of:
a) preparing a fermentation broth containing a pseudomonic acid producing micro-organism; b) regulating pH of the fermentation broth; and c) recovering the pseudomonic acid from the fermentation broth.
- 2. The improved fermentation process of claim 1, wherein the pseudomonic acid is pseudomonic acid A.
- 3. The improved fermentation process of claim 1, wherein the pseudomonic acid producing micro-organism is selected from the group consisting of a Pseudomonas sp. strain, its progenies, a natural variant and a mutant variant of the Pseudomonas sp.
- 4. The improved fermentation process of claim 3, wherein the Pseudomonas sp. strain is NCAIM (P)B 001235 deposited in the National Collection of the Agricultural and Industrial Micro-organisms.
- 5. The improved fermentation process of claim 1, wherein the fermentation is a submerged culture.
- 6. The improved fermentation process of claim 1, wherein the fermentation is maintained at a temperature of about 20° C.-30° C.
- 7. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated during the production phase of fermentation.
- 8. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated between about 5.5-6.0.
- 9. The improved fermentation process of claim 8, wherein the pH of the fermentation broth is regulated at about 5.7.
- 10. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated by feeding the fermentation broth with an assimilable carbon source.
- 11. The improved fermentation process of claim 10, wherein the feeding of the assimilable carbon source is metabolically controlled.
- 12. The improved fermentation process of claim 10, wherein the assimilable carbon source is selected from the group consisting of a dextrose, a glycerol, a vegetal, an animal oil and a fat.
- 13. The improved fermentation process of claim 10, wherein the assimilable carbon source is dextrose.
- 14. The improved process of claim 13, wherein the dextrose level in the fermentation broth is maintained at a level less than about 0.5% (wt/wt).
- 15. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated by concomitant feeding of the fermentation broth with an assimilable carbon source and an acidic solution.
- 16. The improved fermentation process of claim 15, wherein the assimilable carbon source is glycerol and the acidic solution is hydrochloric acid.
- 17. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated by feeding the fermentation broth with a mineral salt.
- 18. The improved fermentation process of claim 17, wherein the mineral salt is magnesium sulphate.
- 19. The improved fermentation process of claim 17, wherein the mineral salt is a calcium chloride solution.
- 20. The improved fermentation process of claim 19, wherein the calcium chloride solution is about 0.1%-0.8% (wt/wt).
- 21. The improved fermentation process of claim 19, wherein the calcium chloride solution is about 0.1% (wt/wt).
- 22. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated by feeding the fermentation broth with an alkali solution.
- 23. The improved fermentation process of claim 22, wherein the alkali solution is selected from the group consisting of sodium hydroxide and potassium hydroxide.
- 24. The improved fermentation process of claim 22, wherein the alkali solution is potassium hydroxide.
- 25. The improved fermentation process of claim 1, wherein the pH of the fermentation broth is regulated by feeding the fermentation broth with an acidic solution.
- 26. The improved fermentation process of claim 25, wherein the acidic solution is selected from the group consisting of nitric acid (HNO3), sulphuric acid (H2SO4) and hydrochloric acid (HCl).
- 27. The improved fermentation process of claim 25, wherein the acidic solution is hydrochloric acid.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims the benefit under 35 U.S.C. §119(e) of the U.S. Provisional Patent Application Serial No. 60/299,927 filed Jun. 21, 2001, the content of which is incorporated herein in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60299927 |
Jun 2001 |
US |