Claims
- 1. An amine protected amino acid-chelate, which is useful as an unnatural amino acid to replace a natural amino acid in the solid phase synthesis of a peptide as a precursor to introduce said unnatural amino acid, which unnatural amino acid is capable of binding a metal atom as a chelate in the peptide chain, said amino acid-chelate hayinc the structure: ##STR7## wherein Y is a direct bond, A is the amine protecting group 9-fluorenylmethylcarbonate (Fmoc);
- Q is selected from a straight chain alkylene, or a branched chain alkylene, having 1 to 10 carbon atoms;
- Q.sup.1 is --OH;
- Z.sup.1, Z.sup.2, Z.sup.3, and Z.sup.4 are identical and selected from --CH.sub.2 --, or --CH.sub.2 CH.sub.2 --;
- R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are identical and selected from --H, or --C(CH.sub.3).sub.3.
- 2. The amino acid-chelate of claim 1 wherein Z.sup.1, Z.sup.2, Z.sup.3, and Z.sup.4 are each --CH.sub.2 --.
- 3. The amino acid-chelate of claim 1 wherein Q is the straight chain alkylene --CH.sub.2 CH.sub.2 CH.sub.2 CH.sub.2 --, and Z.sup.1, Z.sup.2, Z.sup.3 and Z.sup.4 are each --CH.sub.2 --.
- 4. The amino acid-chelate of claim 1 wherein Q is the straight chain alkylene --CH.sub.2 CH.sub.2 CH.sub.2 --.
- 5. The amino acid-chelate of claim 4 wherein Z.sup.1 to Z.sup.4 are each --CH.sub.2 CH.sub.2 --.
- 6. The amino acid chelate of claim 1 wherein R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are each --H.
- 7. The amino acid chelate of claim 6 wherein Z.sup.1, Z.sup.2, Z.sup.3, Z.sup.4 are each --CH.sub.2 --.
- 8. The amino acid chelate of claim 4 wherein Z.sup.1, Z.sup.2, Z.sup.3, and Z.sup.4 are each --CH.sub.2 --.
- 9. The amino acid chelate of claim 1 wherein Q is a branched chain alkylene.
- 10. The amino acid chelate of claim 9 wherein R.sub.1 to R.sub.4 are each --C(CH.sub.3).sub.3.
- 11. The amino acid chelate of claim 9 where R.sup.1 to R.sup.4 are each --H.
- 12. The amino acid chelate of claim 9 wherein Z.sup.1 to Z.sup.4 are each --CH.sub.2 --.
- 13. The amino acid chelate of claim 9 wherein Z.sup.1 to Z.sup.4 are each --CH.sub.2 CH.sub.2 CH.sub.2 CH.sub.2 --.
ORIGIN OF THE INVENTION
The present invention was made in part in research supported by the United States National Institutes of Health Grant # FD 8RI GM 41911A-03-NF-A-03/92. The U.S. Government has rights in this invention.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4647447 |
Gries |
Mar 1987 |
|