The present invention generally relates to the sampling of air, and more particularly relates to the collection of pathogen and aerosol particles from air samples.
There is an increasing demand for air sampling systems for military, private or individual use that are capable of collecting aerosol and pathogen particles or spores. While current air sampling systems have been proven to function reliably, they are often quite large and thus not only consume a great deal of power, but also produce a lot of noise. These systems also tend to produce very large liquid samples, analyses of which can take several days or even weeks. Thus current air sampling systems are not practical for private or individual use, or for environments or circumstances in which analysis of an air sample must be performed quickly.
Therefore, there is a need in the art for a compact, high-efficiency bio-aerosol collector that can produce a relatively small volume of liquid sample for expedited analysis.
Embodiments of the invention generally provide an apparatus for collecting particles (for example, biological aerosol particles) from an air sample comprising an air intake assembly adapted to draw the air sample into the apparatus, a separation section coupled to the intake assembly and adapted to separate aerosol particles from the air sample, a capture section coupled to the separation section and adapted to transport the aerosol particles into a stream of liquid, and a hydrophobic membrane disposed between the separation section and the capture section and adapted to establish a controllable air/fluid boundary therebetween.
Further embodiments of the apparatus comprise an air intake assembly adapted to draw the air sample into the apparatus, a separation section coupled to the intake assembly and adapted to separate aerosol particles from the air sample, a capture section coupled to the separation section and adapted to transport the aerosol particles in a stream of liquid, and a corona charging section disposed between the separation section and the capture section and adapted to focus the aerosol particles into the stream of liquid.
So that the manner in which the above recited embodiments of the invention are attained and can be understood in detail, a more particular description of the invention, briefly summarized above, may be had by reference to the embodiments thereof which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, for the invention may admit to other equally effective embodiments.
To facilitate understanding, identical reference numerals have been used, where possible, to designate identical elements that are common to the figures.
Embodiments of the invention generally provide a compact, lightweight, low power and low noise device capable of collecting respirable airborne particles and focusing them into a small liquid volume. In one embodiment, the device is capable of achieving a particle concentration in the range of approximately 1 to 10 microns, and can achieve sampling rates of up to approximately 1000 liters per minute (lpm).
The air intake assembly 104 is adapted to draw air flow into the collection apparatus 100 and comprises a motor 110, first and second fans 112A, 112B, and an air duct 114. The first fan 112A is disposed proximate a first end 101 of the collection apparatus 100 and is coupled to the fan motor 110. The optional second fan 112B is positioned inward of the first fan 112A along a longitudinal axis of the apparatus 100, and in one embodiment, the second fan 112B is smaller than the first fan 112A. The air duct 114 begins at an aperture 116 in the second end 103 of the apparatus 100 and extends at least partially therethrough to provide an inlet path for the air that is drawn in by the fans 112A, 112B when in operation. In one embodiment, the duct 114 is disposed through the center 105 of the housing 102. Optionally, the air intake assembly 104 may further comprise an impactor 150 positioned between the duct 114 and the fans 112A, 112B and adapted to act as a pre-filter. That is, the impactor 150 includes a plurality of tubes or channels 152 for filtering large particles out of the primary flow as it is drawn into the apparatus 100.
The sample separation section 106 comprises a substantially circular array of cyclones 118 positioned radially outward of the center 105 of the apparatus 100 (i.e., in the embodiment illustrated in
Referring to
The vortex breaker 120 is separated from the capture section 108 by a controllable air/fluid boundary 130. The air/fluid boundary 130 is positioned adjacent the exterior of the vortex chambers 128, and in one embodiment the mechanism comprises a liquid plate 132 having a high porosity hydrophobic membrane 134 disposed thereon. The hydrophobic membrane 134 is adapted to establish a liquid seal or boundary between the vortex chamber 128, which is adapted to contain air or particle flow (i.e., a gaseous medium), and the capture section 108, which is adapted to contain a liquid as described further herein. In one embodiment, the membrane 134 comprises a nylon mesh that is thermally imbedded over at least a portion of the capture section 108. The nylon mesh is optionally treated with polytetrafluoroethylene (PTFE) or an equivalent substance to increase its hydrophobic properties.
Referring to
The air/fluid boundary 130 described above optionally includes an electrostatic focusing mechanism such as a corona charging section 500 for electrostatically manipulating the particles to enhance the focusing of the particles into the liquid in the at least one channel 140 of the capture section 108. One embodiment of a corona charging section 500 is illustrated in a schematic view in
Referring back to
The cyclones 118 separate particles from the primary air flow. As the flow field is rapidly revolved within the cyclone 118, centrifugal force drives the aerosol particles to the walls of the cyclone 118, where the particles may be tribo-charged by rubbing against the wall surface. As the flow continues to spiral through the cyclone 118 to the second end 109, additional particles are separated from the flow. The flow of aerosol particles exits the cyclones 118 through the second ends 109 and enters the chamber 128 of the vortex breaker 120, where it is concentrated into a denser, low velocity flow.
The primary flow reverses direction and flows back through the centers of the cyclones 118, where it passes out of the first ends 107 of the cyclones 118 and is carried past the fans 112A, 112B and through exhaust ports 144 in the first end 101 of the housing 102, to exit the collection apparatus 100. If a precipitator section such as that illustrated in
The densified aerosol flow is drawn through the tangential slots 136 in the walls 138 of the vortex breaker chambers 128. As the particles flow outward from the chambers 128, the particles are electrostatically focused into an array of capillaries formed by the hydrophobic mesh membrane 134. The particles are drawn through the capillaries in the mesh 134 and into the liquid of the capture section 108, where a continuous liquid flow through the microfluidic channels 140 transports the captured particles into the collection chamber 142. Alternatively, the capture section 108 may be coupled to a port or line (not shown) that is adapted to transport the fluid out of the collection apparatus 100 and into, for example, a separate collection container or an analysis device.
As the flow of particles arrives at the air/liquid interface (i.e., the hydrophobic membrane 134), the particles reside in a boundary layer where the liquid flow velocity approaches zero. Particle transport in the liquid is enhanced by positioning the corona electrode (604 in
Over the course of operation, the hydrophobic mesh membrane 134 may become clogged with large particles, dust or debris. In such an instance, the water in the channels 140 may be pressurized to a level exceeding the retention pressure of the mesh membrane 134. Consequently, the boundary established by the membrane 134 will be broken and water will flow out through the mesh 134, carrying dust and debris away with the flow. The water pressure is subsequently reduced, allowing the mesh membrane 134 to re-establish the liquid seal. Thus the hydrophobic membrane 134 may be easily cleaned without having to disassemble the collection apparatus 100.
Although a collection apparatus according to the present invention has been heretofore described as a device having a substantially cylindrical configuration, those skilled in the art will appreciate that a collection apparatus may be constructed in alternate shapes and configurations without departing from the scope of the invention. For example,
The collection apparatus 1000 is constructed as a box having an air inlet side 1004 for the intake of air samples and an air outlet side 1006 opposite the inlet side 1004 for the expulsion of separated primary flow air. The inlet and outlet sides 1004, 1006 have a plurality of apertures 1010 for the intake or expulsion of air. In addition, at least one capture liquid outlet 1008 may be coupled to the housing 1002 to transport liquid and particles captured therein to a collection or analysis device (not shown).
As illustrated in
As in the previous embodiments, the separation section 1012 comprises at least one cyclone 1024 coupled to the inlets 1022 for receiving air samples and separating airborne particles in the samples from the primary flow. The at least one cyclone expels clean primary flow through a first exit port 1040, and expels separated particles through a second exit port 1026.
The second exit port 1026 transports the separated particles to a chamber 1028 of the vortex breaker section 1014, where the particle flow is concentrated for passage to the capture section 1016.
The capture section 1016 is coupled to the vortex breaker section 1014. Concentrated particle flow is passed through an exit port 1030 in the vortex chamber 1028 to a capture section channel 1032. The channel 1032 contains a liquid for transporting the particles to a collection or analysis device (i.e., via the capture liquid outlet 1008 illustrated in
A fourth embodiment of a collection apparatus according to the present invention is illustrated in
Thus, the present invention represents a significant advancement in the field of bio-aerosol collection. An apparatus is provided that achieves high air-sampling rates coupled with high concentration ratios, while minimizing power consumption, size, noise and consumables. Efficiency is further promoted by an air-to-liquid interface membrane that is easily cleaned to enable substantially clog-free operation of the apparatus.
While the foregoing is directed to embodiments of the invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
This application claims the priority of U.S. Provisional Patent Application No. 60/390,974, filed Jun. 24, 2002 (entitled “Ultra-High Concentrating Bio-Aerosol Collector”), and to U.S. Provisional Patent Application No. 60/446,323, filed Feb. 10, 2003 (entitled “Corona-Based Bio-Aerosol Collector”), both of which are herein incorporated by reference.
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