Method and Apparatus for Real-Time Frame Encoding

Abstract
A device incorporates a software-based H.264 video encoder for providing compressed, or encoded, video data. The H.264 encoder incorporates a timer algorithm such that the time available to the H.264 encoder within the macroblock mode decision and motion estimation framework is constrained, or restricted. The particular amount of time available to the H.264 encoder then determines a subset of available encoding modes that the H.264 encoder can use to encode a macroblock.
Description
BACKGROUND OF THE INVENTION

The present invention generally relates to communications systems and, more particularly, to an encoder for use in encoding real-time data, e.g., video.


Real-time H.264 encoding is very computationally demanding and requires a significant amount of memory and computational resources. Traditionally, video encoders are implemented in hardware in the form of an application specific integrated circuit (ASIC). However, due to advancing personal computer (PC) technology, most high-end computers now have the processing power to perform real-time audio and video compression at modest resolutions. In the case of H.264, current high-end general purpose PC processors, such as the Pentium® IV, are able to provide enough processing power to encode Common Intermediate Format (CIF) (352×288 pixels/frame, 30 frames/second) video in real-time.


This shift from hardware-based solutions to software-based solutions for video encoder implementations creates new challenges that must be overcome. More specifically, in a software-based video encoder it is important that each video frame (frame) be encoded within an amount of time that is less than, or equal to, the amount of time allocated for the display of a frame (this amount of time is referred to herein as the display time, or, equivalently, by its inverse—the display rate). However, an H.264 encoder motion estimation algorithm may use an iterative algorithm that iteratively refines its motion search until it either reaches a satisfactory threshold (i.e., a close match) or exhausts all possible search points. As such, execution time is not constant and may vary widely. Also, software implementations are by their nature less deterministic in execution performance than their hardware counterparts, resulting in further execution time variations. For example, processor speed directly affects software execution time. Thus, in a software-based video encoder the encoding of some frames may be faster than the display rate, while the encoding of other frames may be slower than the display rate. As long as the average encoding speed is equal to the display rate over a short latency window, the video encoder will perform as required. However, if the average encode speed drops below the display rate over the short latency window, then the video encoder will not perform as required resulting in possible dropped frames and decreased performance.


SUMMARY OF THE INVENTION

We have observed that it is possible to constrain the operation of a video encoder such that execution time is limited and, therefore, more deterministic without detrimentally affecting performance. As a result, such a constrained video encoder performs as required and encodes video frames, on average, at least as fast as the display rate, if not faster. Therefore, and in accordance with the principles of the invention, a video encoder assigns an amount of time for processing a macroblock of data; and selects a subset of available encoding modes as a function of the assigned amount of time; wherein at least one of the encoding modes in the selected subset is used for processing the macroblock.


In an embodiment of the invention, a device incorporates a software-based H.264 video encoder for providing compressed, or encoded, video data. The H.264 encoder incorporates a timer algorithm such that the time available to the H.264 encoder within the macroblock mode decision and motion estimation framework is constrained, or restricted. The particular amount of time available to the H.264 encoder then determines a subset of available encoding modes that the H.264 encoder can use to encode a macroblock. As a result, a method is provided to enforce real-time frame encoding without greatly affecting the quality of the encoded video.





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 shows an illustrative device in accordance with the principles of the invention;



FIG. 2 shows an illustrative block diagram of an H.264 encoder embodying the principles of the invention;



FIG. 3 shows an illustrative flow chart for use in a video encoder in accordance with the principles of the invention;



FIGS. 4-5 show illustrative techniques for detecting whether or not to constrain encoding in accordance with the principles of the invention;



FIGS. 6-7 show other illustrative flow charts for use in a video encoder in accordance with the principles of the invention; and



FIG. 8 shows a table of subsets of encoding modes in accordance with the principles of the invention.





DETAILED DESCRIPTION

Other than the inventive concept, the elements shown in the figures are well known and will not be described in detail. Also, familiarity with video broadcasting, receivers and video encoding is assumed and is not described in detail herein. For example, other than the inventive concept, familiarity with current and proposed recommendations for TV standards such as NTSC (National Television Systems Committee), PAL (Phase Alternation Lines), SECAM (SEquential Couleur Avec Memoire) and ATSC (Advanced Television Systems Committee) (ATSC) is assumed. Likewise, other than the inventive concept, transmission concepts such as eight-level vestigial sideband (8-VSB), Quadrature Amplitude Modulation (QAM), and receiver components such as a radio-frequency (RF) front-end, or receiver section, such as a low noise block, tuners, demodulators, correlators, leak integrators and squarers is assumed. Similarly, other than the inventive concept, formatting and encoding methods (such as Moving Picture Expert Group (MPEG)-2 Systems Standard (ISO/IEC 13818-1)) and, in particular, H.264: International Telecommunication Union, “Recommendation ITU-T H.264: Advanced Video Coding for Generic Audiovisual Services,” ITU-T, 2003, for generating bit streams are well-known and not described herein. In this regard, it should be noted that only that portion of the inventive concept that is different from known H.264 encoding is described below and shown in the figures. As such, H.264 video encoding concepts of pictures, frames, fields, macroblocks, luma, chroma, Intra-frame prediction, Inter-frame prediction, etc., is assumed and not described herein. It should also be noted that the inventive concept may be implemented using conventional programming techniques, which, as such, will also not be described herein. Finally, like-numbers on the figures represent similar elements.


An illustrative embodiment of a device 105 in accordance with the principles of the invention is shown in FIG. 1. Device 105 is representative of any processor-based platform, e.g., a PC, a server, a personal digital assistant (PDA), a cellular telephone, etc. In this regard, device 105 includes one or more processors with associated memory (not shown). Device 105 includes H.264 encoder 150. Other than the inventive concept, H.264 encoder 150 receives a video signal 149 (which is, e.g., derived from input signal 104) and, in accordance with ITU-T H.264 (noted above), provides an H.264 encoded video signal 151. The latter may be included as a part of an output signal 106, which represents an output signal from device 105 to, e.g., another device, or network (wired, wireless, etc.). It should be noted that although FIG. 1 shows that H.264 encoder 150 is a part of device 105, the invention is not so limited and H.264 encoder 150 may be external to device 105, e.g., physically adjacent, or deployed elsewhere in a network (cable, Internet, cellular, etc.) such that device 105 can use H.264 encoder 150 for providing an H.264 encoded video signal. For the purposes of this example only, it is assumed that video signal 149 is a real-time video signal conforming to a CIF (352×288 pixels/frame, 30 frames/second) video format.


An illustrative block diagram of H.264 encoder 150 is shown in FIG. 2. Illustratively, H.264 encoder 150 is a software-based video encoder as represented by processor 190 and memory 195 shown in the form of dashed boxes in FIG. 2. In this context, computer programs, or software are stored in memory 195 for execution by processor 190. The latter is representative of one or more stored-program control processors and does not have to be dedicated to the video encoder function, e.g., processor 190 may also control other functions of device 105. Memory 195 is representative of any storage device, e.g., random-access memory (RAM), read-only memory (ROM), etc.; may be internal and/or external to processor H.264 encoder 150; and is volatile and/or non-volatile as necessary. As known in the art, H.264 encoded video signal 151 has two layers as represented by video coding layer 160 and network abstraction layer 165. Other than the inventive concept, video coding layer 160 provides an encoded signal 161, which comprises the video coded data as known in the art, e.g., video sequence, picture, slice and macroblock. Video coding layer 160 comprises an input buffer 180, an encoder 170 and an output buffer 185. The input buffer 180 stores video data from video signal 149 for processing by encoder 170. Other than the inventive concept, described below, encoder 170 compresses the video data in accordance with H.264 and provides compressed video data to output buffer 185. The latter provides the compressed video data as encoded signal 161 to the network abstraction layer 165, which formats the encoded signal 161 in a manner that is appropriate for conveyance on a variety of communications channels or storage channels to provide H.264 video encoded signal 151. For example, network abstraction layer 165 facilitates the ability to map encoded signal 161 to transport layers such as RTP (real-time protocol)/IP (Internet Protocol), file formats (e.g., ISO MP4 (MPEG-4 standard (ISO 14496-14)) for storage and MMS), H.32X for wireline and wireless conversational services), MPEG-2 systems for broadcasting services, etc.


As noted earlier, a software-based video encoder may not perform as required because of the variability in execution time. In particular, a video encoder must encode video frames (frames) either faster than or equal to the display rate. Since the time to encode a frame in a software-based video encoder may vary widely because of algorithm design and processor speed, the software-based video encoder may not perform as required resulting in possible dropped frames and decreased performance. However, we have observed that it is possible to constrain the operation of a video encoder such that execution time is limited and, therefore, more deterministic without detrimentally affecting performance. As a result, such a constrained video encoder performs as required and encodes frames, on average, at least as fast as the display rate, if not faster. Therefore, and in accordance with the principles of the invention, a video encoder assigns an amount of time for processing a macroblock of data; and selects a subset of available encoding modes as a function of the assigned amount of time; wherein at least one of the encoding modes in the selected subset is used for processing the macroblock. In this regard, video coding layer 160 of H.264 encoder 150 incorporates the inventive concept.


An illustrative flow chart is shown in FIG. 3 that outlines one implementation of the inventive concept in the context of an H.264 encoder. In this example, video coding layer 160, of H.264 encoder 150, utilizes a timer algorithm during Inter-frame prediction, i.e., during the macroblock mode decision and motion estimation framework of H.264 encoding. The macroblock mode decision portion of the video encoding algorithm is illustratively chosen as the target for time control because it consumes a greater portion of encoding time than any other element of H.264, and because it exhibits the largest amount of variance in encoding time. In addition, the iteratively refining nature of the H.264 macroblock mode decision algorithm allows the time control method to break out of the macroblock mode decision loop early and still deliver a valid video bit stream with adequate compression performance. As a result, implementation of the time constraint algorithm within the mode decision and motion estimation framework provides a method to enforce real-time frame encoding without greatly affecting the quality of the encoded video.


Referring now to FIG. 3 in more detail, video coding layer 160 begins Inter-frame prediction for a frame of video signal 149 in step 205. In step 210, a check is made if video coding layer 160 should be constrained when encoding macroblocks of the frame, If video coding layer 215 does not have to be constrained, then video coding layer 160 encodes macroblocks as known in the art in step 215. However, if video coding layer 160 does have to be constrained, then a timer algorithm is enabled to constrain the encoding of macroblocks in step 220. The timer algorithm (described further below) is implemented such that it constrains the amount of time that video coding layer 160 spends within the macroblock mode decision and motion estimation framework.


Turning briefly to FIGS. 4 and 5, some examples are shown for use in step 210 for determining whether or not to constrain encoding. In the context of FIG. 4, one method of determining whether or not to constrain encoding utilizes the amount, or level, of data stored in input buffer 180. In particular, if the amount of data stored in the input buffer 180 exceeds a predetermined threshold 506 (as represented by diagonal shading 518), then input buffer 180 provides a “buffer full” signal 507 to processor 190. The “buffer full” signal 507 represents, in effect, whether or not the video data is being processed fast enough by video encoder 170. In the context of FIG. 5, another method of determining whether or not to constrain encoding utilizes the amount, or level, of data stored in output buffer 185. In particular, if the amount of data stored in the output buffer 185 drops below a predetermined threshold 511 (as represented by diagonal shading 519), then output buffer 185 provides a “buffer empty” signal 512 to processor 190. The “buffer empty” signal 512 represents, in effect, whether or not the encoded video data is being provided fast enough by video encoder 170. In addition, it should be noted that the techniques shown in FIGS. 4 and 5 may also be combined, e.g., video encoding may be constrained only if the input buffer 180 if full and the output buffer 185 is empty. However, it should be noted that the inventive concept is not so limited and other mechanisms can be used to detect the possibility of inadequate performance levels such that it is desirable to start constrained encoding.


Referring now to FIG. 6, a more detailed view of step 220 of FIG. 3 is shown. In step 305, an amount of time, TF, is allotted to encoding of the frame. This initial value of TF represents the amount of time available for processing all of the macroblocks in the frame. The value of TF is less than the display time. In the context of CIF video, TF is less than 0.033 seconds. In this allocation, encoder overhead should be determined, or approximated, so that TF should not include processing time that is not related to mode decision and motion estimation. Other than the inventive concept, encoder overhead can be determined adaptively using timers and averaging buffers as known in the art. It should be noted that the amount of time, TF, may also be further adjusted depending upon the levels of the input buffer 180 and/or the output buffer 185. In step 310, a macroblock of the frame is encoded (described below) in accordance with the principles of the invention. In step 320, a check is made if all of the macroblocks have been processed. If some macroblocks still remain, execution proceeds to step 315, where the value for TF is further reduced by the amount of time taken to process the previous macroblock. In other words, TF now represents the amount of time left to process the remaining macroblocks of the frame. Once all of the macroblocks of the frame have been processed, execution ends.


In step 310, macroblocks of a frame are processed in a constrained fashion in accordance with the principles of the invention. An illustrative flow chart for use in step 310 is shown in FIG. 7. In step 405, an amount of time, TM, is allocated for processing a macroblock. This amount of time, TM, is derived from TF. For example, TM may be simply determined by dividing TF by the number of macroblocks that have to be processed. For simplicity, the amount of time, TM, is described herein in the context of “time units.” Time units may be determined empirically. For the purposes of this example, a time unit is set equal to 20 microseconds for a Pentium® IV based system with a clock frequency of about 3 gigahertz. In accordance with the principles of the invention, the particular amount of time, TM, available for processing a macroblock determines a subset of available encoding modes that the H.264 encoder can use to encode the macroblock. As a result, the inventive concept provides a method to enforce real-time frame encoding without greatly affecting the quality of the encoded video.


Before continuing with a description of the flow chart of FIG. 7, attention should briefly be directed to table 605 of FIG. 8. In accordance with the principles of the invention, the macroblock encoding modes of H.264 are divided into a number of subsets, each subset associated with an amount of encoding time. This is illustrated in table 605. For example, as can be observed from table 605, subset encoding mode A requires 2 time units and comprises the 16×16 macroblock mode. As such, if the video encoder selects subset encoding mode A, the 16×16 macroblock mode is checked for the macroblock. As illustration only, the assignments shown in table 605 are used in the flow chart of FIG. 7.


Turning back to FIG. 7, in step 410, video encoder 170 checks skip mode as known in the art. In particular, the video encoder determines the distortion and output bits if the video encoder does choose the skip mode. Execution then proceeds to step 415, which checks the value of TM to determine if at least TA time units remain for processing the macroblock. If at least TA time units do not remain then execution proceeds to step 455 (described below). However, if at least TA time units do remain, then the video encoder checks the encoding, modes associated with subset A in step 420. That is, the video encoder encodes the macroblock into a number of coded bits using the encoding modes for the selected subset. For example, using the associations shown in table 605 of FIG. 8, the video encoder encodes the macroblock using the 16×16 macroblock mode. After encoding, the amount of time taken to encode the macroblock is subtracted from TM in step 425.


In step 430, a check is made of TM to determine if at least TB time units remain for processing the macroblock. If at least TB time units do remain, then the video encoder checks the encoding modes associated with subset B in step 435. That is, the video encoder encodes the macroblock into a number of coded bits using the encoding modes for the selected subset. For example, using the associations shown in table 605 of FIG. 8, the video encoder encodes the macroblock using the four macroblock modes: 8×8, 4×8, 8×4 and 4×4.


However, if at least TB time units do not remain in step 430 then execution proceeds to step 440. In the latter step, a check is made of TM to determine if at least TC time units remain for processing the macroblock. If at least TC time units do not remain then execution proceeds to step 455 (described below). However, if at least TC time units do remain, then the video encoder checks the encoding modes associated with subset C in step 445. For example, using the associations shown in table 605 of FIG. 8, the video encoder encodes the macroblock using the 8×8 macroblock mode. If either subset B or subset C were selected, the amount of time taken to encode the macroblock is subtracted from TM in step 450.


In step 455, a check is made of TM to determine if at least TD time units remain for processing the macroblock. If at least TD time units do not remain then execution proceeds to step 480 (described below). However, if at least TD time units do remain, then the video encoder checks the encoding modes associated with subset D in step 460. For example, using the associations shown in table 605 of FIG. 8, the video encoder encodes the macroblock using the 16×8 macroblock mode. After encoding, the amount of time taken to encode the macroblock is subtracted from TM in step 465.


In step 470, a check is made of TM to determine if at least TE time units remain for processing the macroblock. If at least TE time units do not remain then execution proceeds to step 480 (described below). However, if at least TE time units do remain, then the video encoder checks the encoding modes associated with subset E in step 4475. For example, using the associations shown in table 605 of FIG. 8, the video encoder encodes the macroblock using the 8×16 macroblock mode.


In step 480, a check is made to determine if any subsets were selected for encoding the macroblock. If no subsets were selected because of insufficient processing time being available, then the macroblock is skipped and execution proceeds to step 320 of FIG. 6. However, if at least one subset was selected, then, in step 485, the video encoder selects an encoding mode from the selected subsets. This selected encoding mode is the one that is actually used for encoding the macroblock for transmission and/or storage. In particular, for each encoding mode of the selected subsets, the video encoder counts the number of output coded bits and calculates the distortion between a re-constructed macroblock (derived from the output coded bits) and the original macroblock using any one of a number of known techniques. For example, tie video encoder can use the sum of absolute differences (SAD) between the re-constructed macroblock and the original macroblock; the sum of square error (SSE) distortion between the re-constructed macroblock and the original macroblock; or a measure of the root mean square (RMS). Following this, a rate-distortion optimization (RDO) metric is calculated as known in the art for each encoding mode. In particular, the RDO combines the output coded bits and the calculated distortion factors for each encoding mode. For example: RDO=Coef_A * Number_Coded_Bits+Coef_B * Distortion; where “Coef_A” and “Coef_B” are weighting coefficients, which can be determined empirically, “Number_Coded_Bits” is the number of output coded bits for an encoding mode and “Distortion” is the calculated distortion factor for the encoding mode. Once each of the RDO values is determined, the video encoder selects the encoding mode with the smaller RDO as the actual encoding mode. After selecting the actual encoding mode, execution proceeds to step 320.


As described above, and in accordance with the inventive concept, a video encoder may trade-off coding efficiency for a more deterministic execution time by constraining video encoder operation. Thus, the video encoder adaptively maintains real-time encoding performance. In particular, the time constraint algorithm described above basically eliminates choices if there is not enough time left to process a macroblock. For example, in the context of the embodiment described above, if the video encoder only has enough time to check the 16×16 and 16×8 modes for a macroblock, then the video encoder will choose the best of these two modes instead of checking other modes. Thus, and in accordance with the principles of the invention, it is possible to enforce a maximum frame encode time with little, if no, decrease in encoding efficiency. Indeed, by using this method. more than 95 percent of all frames will be encoded within the time assigned, thus enabling an encoder to maintain real-time performance regardless of the input. For example, a constrained video encoder may only increase the encoded bit stream by 2-3% in size but can save 15-20% in time to guarantee real-time operation.


It should be noted that although the inventive concept was illustrated in the context of H.264 Inter-frame prediction, the inventive concept is not so limited and is applicable to other portions of H.264 as well as other types of video encoding. For example, the inventive concept is also applicable to Intra-frame prediction. Further, although illustrated in the context of dynamically selecting the constrained encoding mode as represented by step 210 of FIG. 3, this step can be eliminated and a video encoder can be simply operated in a constrained mode in accordance with the principles of the invention. In fact, a constrained mode may be a configurable option in the video encoder. It should also be noted that the other variations are possible. For example, the number of reference frames that are used for motion estimation purposes can be adjusted based on available time units. Likewise, if there are a limited amount of time units allocated to a macroblock, then only the most recent reference picture may be used. Further, known complexity metrics of the image and macroblocks, such as the sum of absolute differences (SAD) between two blocks, and/or variance, may also be used as factors for allocating time units and encoding modes.


In view of the above, the foregoing merely illustrates the principles of the invention and it will thus be appreciated that those skilled in the art will be able to devise numerous alternative arrangements which, although not explicitly described herein, embody the principles of the invention and are within its spirit and scope. For example, although illustrated in the context of separate functional elements, these functional elements may be embodied in one or more integrated circuits (ICs). Similarly, although shown as separate elements, any or all of the elements may be implemented in a stored-program-controlled processor, e.g., a digital signal processor, which executes associated software, e.g., corresponding to one or more of the steps shown in, e.g., FIGS. 3, 6 and/or 7, etc. Further, the principles of the invention are applicable to other types of communications systems, e.g., satellite, Wireless-Fidelity (Wi-Fi), cellular, etc. Indeed, the inventive concept is also applicable to stationary or mobile receivers. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments and that other arrangements may be devised without departing from the spirit and scope of the present invention as defined by the appended claims.

Claims
  • 1. An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO:32, SEQ ID NO:24, SEQ ID NO:41, SEQ ID NO:43, and SEQ ID NO:42.
  • 2. An isolated nucleic acid consisting essentially of a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO:32, SEQ ID NO:24, SEQ ID NO:41, SEQ ID NO:43, and SEQ ID NO:42.
  • 3. An isolated nucleic acid consisting essentially of a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO:32, SEQ ID NO:24, SEQ ID NO:41, SEQ ID NO:43, and SEQ ID NO:42, wherein said nucleotide sequence further has from one to four mismatches and hybridizes under NASBA hybridization conditions, to the 3′NTR of HCV.
  • 4. An isolated nucleic acid comprising a nucleotide sequence set forth in SEQ ID NO: 37.
  • 5. An isolated nucleic acid consisting essentially of a nucleotide sequence set forth in SEQ ID NO:37.
  • 6. An isolated nucleic acid comprising (a) a tag oligonucleotide having a 5′ end and a 3′ end, and (b) a primer nucleic acid having a 5′ end and a 3′ end and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO: 8, wherein the tag oligonucleotide consists of about 18 to about 23 randomly selected nucleotides heterologous to HCV and is linked at its 3′ end to the 5′ end of the primer nucleic acid.
  • 7. The isolated nucleic acid of claim 6, wherein the tag oligonucleotide comprises the nucleotide sequence set forth in SEQ ID NO: 9.
  • 8. An isolated nucleic acid comprising (a) a promoter oligonucleotide that, when in double-stranded form, can function as a T7 promoter, and (b) a primer nucleic acid having a 5′ end and a 3′ end and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:15, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32, wherein the promoter oligonucleotide is linked at its 3′ end to the 5′ end of the primer nucleic acid.
  • 9. The isolated nucleic acid of claim 8, wherein the promoter oligonucleotide has the nucleic acid sequence set forth in SEQ ID NO:38.
  • 10. The isolated nucleic acid of claim 8, wherein the promoter oligonucleotide has the nucleic acid sequence set forth in SEQ ID NO:23.
  • 11. A method of assaying for the presence of HCV in a nucleic acid sample comprising a. Amplifying a selected HCV nucleic acid to form an HCV amplification product, utilizing as a primer of amplification a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 24; SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO:32, SEQ ID NO: 41, SEQ ID NO: 42, and SEQ ID NO:43; andb. Detecting the presence of amplified product.
  • 12. The method of claim 11, wherein the amplification utilizes a first primer comprising the nucleotide sequence set forth in SEQ ID NO: 11 and a second primer comprising the nucleotide sequence set forth in SEQ ID NO:8.
  • 13. The method of claim 12, wherein the first primer further comprises, at its 5′ end, a promoter oligonucleotide that, when in double-stranded form, can function as a T7 promoter.
  • 14. The method of claim 13, wherein the T7 promoter is SEQ ID NO: 38.
  • 15. The method of claim 11, wherein the amplification utilizes a first primer comprising the nucleotide sequence set forth in SEQ ID NO: 15 and a second primer comprising the nucleotide sequence set forth in SEQ ID NO:8.
  • 16. The method of claim 15, wherein the first primer further comprises, at its 5′ end, a promoter oligonucleotide that, when in double-stranded form, can function as a T7 promoter.
  • 17. The method of claim 11, wherein the amplification utilizes a first primer and a second primer, said second primer comprising (a) a tag oligonucleotide having a 5′ end and a 3′ end, and (b) a primer nucleic acid having a 5′ end and a 3′ end and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO: 8, wherein the tag oligonucleotide consists of about 10 to about 25 randomly selected nucleotides heterologous to HCV and is linked at its 3′ end to the 5′ end of the primer nucleic acid.
  • 18. The method of claim 11, wherein the amplification utilizes a first primer and a second primer, said first primer comprising (a) a promoter oligonucleotide that, when in double-stranded form, can function as a T7 promoter, and (b) a primer nucleic acid having a 5′ end and a 3′ end and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 11, SEQ ID NO:15, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32, wherein the promoter oligonucleotide is linked at its 3′ end to the 5′ end of the primer nucleic acid.
  • 19. The method of claim 11, wherein the detecting is performed by hybridizing to the amplification product a detectable probe.
  • 20. The method of claim 19, wherein the detectable probe comprises a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 41, SEQ ID NO: 43, and SEQ ID NO: 44.
  • 21. The method of claim 11, wherein the detecting is performed by capturing the amplification product on an immobilised capture probe.
  • 22. The method of claim 21, wherein the capture probe comprises a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 19.
  • 23. The method of claim 11, wherein the amplification is performed by isothermal amplification or thermocyclic amplification.
  • 24. A method of assaying for the presence of HCV in a nucleic acid sample comprising a. Amplifying a target nucleic acid comprising a selected portion of the 3′NTR of HCV andb. Detecting the presence of amplified product utilizing a probe comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 21, SEQ ID NO: 22; SEQ ID NO:19, SEQ ID NO: 24, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 44.
  • 25. A method of detecting the presence of HCV nucleic acid in a sample comprising hybridizing with the sample, under selective hybridization conditions, a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32.
  • 26. A method of quantifying the amount of HCV nucleic acid in a sample comprising a. Amplifying selected HCV nucleic acid to form an HCV amplification product, utilizing as a primer of amplification a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 8, SEQ ID NO:11, SEQ ID NO: 15, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 24; SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO:32, SEQ ID NO: 41, SEQ ID NO: 42, and SEQ ID NO:43, andb. Quantifying the amount of amplified product.
  • 27. The method of claim 26, wherein the amplification utilizes a first primer comprising the nucleotide sequence selected from the group consisting of SEQ ID NO:11 and SEQ ID NO: 15 and a second primer comprising the nucleotide sequence set forth in SEQ ID NO:8.
CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application No. 60/647,824, filed Jan. 28, 2005.

PCT Information
Filing Document Filing Date Country Kind 371c Date
PCT/US2006/002415 1/24/2006 WO 00 7/27/2007
Provisional Applications (1)
Number Date Country
60647824 Jan 2005 US