Claims
- 1. A method for differentiating first and second subpopulations of blood cells in a blood sample, said first and second subpopulations of blood cells being of similar volume, electrical conductivity and/or light scattering properties, said method comprising the steps of:a) providing a plurality of microspheres having a reactant bonded thereto, said reactant specifically binding to a moiety present on only the blood cells of said first subpopulation, said microspheres having a size between about 0.65 and 3.0 microns; b) mixing said microspheres with said blood sample to cause a plurality of said microspheres to bind to each of the blood cells of said first subpopulation, whereby a plurality of microsphere/cell complexes are formed in said blood sample; and c) differentiating said complexes from unbound blood cells in said blood sample by passing said complexes and unbound blood cells seriatim through a sensing zone while measuring the respective volume, electrical conductivity and light scattering properties of said complexes and blood cells as each passes through said zone.
- 2. The method as defined by claim 1 wherein said differentiating step comprises producing at least a two dimensional characterization of said complexes and said blood cells representing their respective light scattering and electrical properties.
- 3. The method as defined by claim 1 wherein said light scattering property is measured by irradiating each of said complexes and unbound blood cells as it passes through said zone with a beam of radiation and measuring the intensity of scattered light between 10 and 70 degrees with respect to the direction of said beam of radiation.
- 4. The method as defined by claim 1 wherein said electrical conductivity is measured by subjecting said complexes/unbound cells to an RF electric field.
- 5. A method for differentiating first and second subpopulations of blood cells in a blood sample, said first and second subpopulations of blood cells being of similar volume, electrical conductivity and/or light scattering properties, said method comprising the steps of:a) providing a plurality of microspheres having a reactant bonded thereto, said reactant specifically binding to a moiety present on only the blood cells of said first subpopulation, said microspheres having a size between about 0.65 and 3.0 microns; b) mixing said microspheres with said blood sample to cause a plurality of said microspheres to bind to each of the blood cells of said first subpopulation, whereby a plurality of microsphere/cell complexes are formed in said blood sample; and c) differentiating said complexes from unbound blood cells in said blood sample by passing said complexes and unbound blood cells seriatim through a sensing zone and at which each of said complexes and unbound blood cells is irradiated by a beam of light and subjected to an electrical field, whereby the respective volume and/or electrical conductivity of said complexes and unbound blood cells can be determined substantially simultaneously with the light scattering properties as each complex and unbound blood cell passes through said zone.
- 6. The method as defined by claim 5 wherein said differentiating step comprises producing at least a two dimensional characterization of said complexes and unbound blood cells representing their respective volumes and light scattering properties.
- 7. The method as defined by claim 5 wherein said differentiating step comprises producing at least a two dimensional characterization of said respective electrical conductivities and light scattering properties.
- 8. The method as defined by claim 5 wherein said light scattering property is measured by irradiating each of said complexes and unbound blood cells as it passes through said zone with a beam of radiation and measuring the intensity of scattered light between 10 and 70 degrees with respect to the direction of said beam of radiation.
- 9. The method as defined by claim 5 wherein said volume is determined by subjecting each of said complexes and unbound cells to a DC electric field while said cell passes through said sensing zone.
- 10. The method as defined by claim 5 wherein said electrical conductivity is determined by subjecting each of said complexes and unbound cells to an RF electric field as each of said complexes and unbound cells passes through said sensing zone.
- 11. The method as defined by claim 5 wherein said first and second subpopulations of blood cells are lymphocytes, and wherein said first subpopulation of lymphocytes are of a type selected from the group consisting of CD2, CD4 and CD8 cells.
Parent Case Info
This is a continuation of application Ser. No. 07/617,075 filed Nov. 23, 1990, now abandoned.
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Continuations (1)
|
Number |
Date |
Country |
Parent |
07/617075 |
Nov 1990 |
US |
Child |
08/303924 |
|
US |