This invention relates to the field of beam steering where light from an illumination source is directed at high rates to a destination. Particular applications include laser scanning confocal microscope, optical scanning, optical particle tracking and light projection systems.
A number of emerging technologies are incorporating photonics. Among these are optical imaging, telecommunications, entertainment devices, image projection systems, medical diagnosis and treatment, photolithography, materials inspection, biosensors, and surveillance. Applications of these technologies share a requirement for the rapid and accurate scanning of a laser beam either to image an object or to project light onto a surface.
When dynamic systems and processes are imaged optically, the rate of image acquisition (i.e. number of image frames acquired per unit time) is an important consideration. Such systems may involve stationary objects that change over time, specimens that move spatially within a field-of-view, or both processes occurring simultaneously. Many important processes occur within time domains that are less than one second, and in such cases, it is frequently desirable to acquire images in at least two spatial dimensions as rapidly as is consistent with sampling sufficient photons to form an acceptable image.
Many imaging applications of dynamic systems and processes also require optimal spatial resolution. Laser scanning confocal microscopy is commonly used to improve this parameter, particularly in the (z) dimension parallel to the optical axis. In scanning microscope systems, such as laser scanning confocal systems, the illuminating light or specimen must be moved relative to one another. This can be accomplished by moving the specimen while keeping the light in a fixed position, by moving the light across the specimen while the latter is kept stationary, or by a moving both the illumination light and the specimen. Certain optical advantages can be achieved by keeping the illumination light stationary and moving the specimen (for example see U.S. Pat. No. 3,013,457, which provides the original description of a confocal optical system). However, this approach involves moving the relatively large mass of a microscope stage or another type of inspection platform, which typically prevents scanning at rates greater than a few frames per second. In addition, this approach restricts use of immersion objectives, where an intermediate layer of an appropriate medium, such as oil, water, or glycerin, must be maintained between the objective and the specimen. Because of such limitations, it is common to scan the illumination light (typically a laser beam) over the specimen in a two-dimensional raster pattern (involving one dimensional lines repeated with intervening steps in the orthogonal dimension) in the majority of modern scanning microscopes. The laser beam is scanned using mirrors mounted on devices capable of controlled motion, such as galvanometers, piezoelectric elements or microelectromechanical system (MEMS) micro-mirrors. In another approach, stationary devices, such as acustooptical beam deflectors (AOD), which use changes in refractive index to alter the path of the light beam, have been implemented as beam-steering devices. However, each of these beam-steering devices is constrained by limitations related to achievable scan rates and/or optical properties.
Galvanometers are currently the beam-steering device most commonly employed in scanning optical systems. Mirrors mounted on two independent galvanometers are used to achieve beam steering in two (x and y) spatial dimensions. Closed loop galvanometer pairs have been used most frequently to take advantage of the ability to modulate and control the position of each mirror as it is moved back and forth in a single dimension in an accurate manner that is inherent to this type of device. In addition, closed-loop galvanometers typically have position feedback signals, which can be used to verify the position of the mirror at a given point in time. However, the frequency response of this type of galvanometer is limited (generally to <1 kHz) by several factors and this restricts image acquisition rates to typically less than video rates. These factors include the extent of mechanical movement of the mirror and the size and, therefore, the mass of the reflective surface required, and ultimately, the time required to dissipate heat resulting from the electromagnetic forces used to drive movements of the mirror. All of these factors are inversely related to the frequency response of the galvanometer system. In another approach, resonant galvanometers, which due to lower friction associated with their movement, can be driven at frequencies of up to ˜8 kHz, have been used to deflect a laser beam in one spatial dimension, while a slower (30-60 Hz) closed loop galvanometer is used to deflect the beam in the second spatial dimension. Using this combination of galvanometers, acquisition rates of 30-60 frames/sec have been achieved for two-dimensional images. (For examples see: Tsien and Bacskai, Video-Rate Confocal Microscopy, Ch. 29 in Handbook of Biological Confocal Microscopy, 2nd ed., J. B. Pawley, Ed., Plenum Press, New York, 1995 and U.S. Pat. No. 5,283,433)
Prior-art systems cannot place the axis of a primary deflection surface in a telecentric conjugate image plane when using two mirrors. The need to utilize physically separate mirrors in galvanometer-based systems to steer the laser beam in two spatial dimensions in galvanometer-based systems imposes optical limitations (for example see discussion by E. H. K. Stelzer in: The Intermediate Optical System of Laser-Scanning Confocal Microscopes, Ch. 9, in Handbook of Biological Confocal Microscopy, 2nd ed., J. B. Pawley, Ed., Plenum Press, New York, 1995). In imaging situations, where laser scanning confocal microscope systems utilizing single photon excitation are used, it is necessary to sense light originating in the sample, such as fluorescent or reflected light, using a fixed spot detector, such as a photomultiplier tube or photodiode. To focus light from the sample onto a fixed point, it must be de-scanned by the beam-steering device. Such de-scanning is optimal when the axis of the primary deflecting surface is placed at a telecentric conjugate image plane. However, such placement is not possible when separate reflective surfaces are used to deflect the beam in each of the two dimensions. Placement of the reflective surfaces in an axial parallel arrangement reduces, but does not eliminate the associated optical distortion. Consequently, it is preferred to utilize a single reflective surface to deflect the beam in both spatial dimensions.
Another approach to rapid, single-axis laser beam deflection involves the use of an acustooptical beam deflector (AOD). As noted previously, this device uses changes in refractive index to deflect the beam rapidly (with a 1-5 kHz frequency range) in one spatial dimension. As is the case for the resonant galvanometer, a second device is required to deflect the beam in the second spatial dimension. In addition, although scanning systems utilizing AOD devices have achieved high scan rates over a somewhat limited range of deflection angles, use of the AOD introduces optical disadvantages, particularly when used with laser scanning confocal microscope systems. These disadvantages include reduced transmission efficiency, wavelength dependent angles of deflection, and the inability of light emitted from the sample at wavelengths greater than that shone on the sample (e.g. fluorescence) to be de-scanned by the AOD device along the optical path used by the illuminating light. Additional optics are required to reduce the impact of these disadvantages on spatial resolution and this decreases the optical efficiency that can be achieved.
High-rate (1-10 kHz frequencies), 2-axis beam deflection has been achieved using electrostatically actuated MEMS micro-mirror beam-steering devices. However, the low level of torque produced by these devices limits the size of the reflective surface to typically <1 mm. Such a small clear aperture limits the achievable spatial resolution to much less than that of confocal systems currently available commercially, and places important limitations on the properties of the intermediate optical system that can be used. Increasing the size of the mirror results in marked reductions in scan frequency and increases in dynamic deformations of reflective surfaces. These deformations diminish the quality of the reflected light and thus, the optical quality of acquired images.
Thus, there is currently a need for a 2-axis beam-steering device having a single, large reflective surface.
The present invention provides 2-axis beam-steering devices having large clear apertures capable of deflecting laser beams or other illumination sources in two dimensions with a frequency response in the kHz range. The present invention involves the use of micro-machined and/or semiconductor structures to formula reflector platform hybridized with closed-loop galvanometers to achieve rapid beam-steering movements. An advantage of the invention is that it permits the use of galvanometer actuators having suitable torque generating capabilities to drive a single reflective surface in two spatial axes. Galvanometers are particularly well suited for being driven by amplitude-modulated sine waves employed as mirror position command signals as described in U.S Utility patent application Ser. No. 10/795,205, filed Mar. 4, 2004, incorporated herein by reference in its entirety, to extend achievable frequency ranges. In addition, the use of the single reflective surface operating in a dual-axis mode optimizes the achievable spatial resolution. A further advantage of the invention is the generation of dual-axis position feedback signals that can be used to monitor and further increase the accuracy of beam-steering. Implementation of these devices in a laser scanning confocal microscope system (LSCMS) is described as a preferred embodiment.
The present invention is depicted in the drawings and will be described below with reference to the Figures, in which:
In an embodiment, the present invention provides a 2-axis, beam-steering device having the following properties:
Devices that meet or exceed these criteria are described below. The torque required to move a large mirrored surface through a significant mechanical angle can be achieved using two or more closed-loop galvanometers to drive a single reflective surface. An example of a suitable galvanometer is Model 6215 commercially available from Cambridge Technology (Cambridge, Mass.). With modifications to the electronic control circuitry, cooling of the galvanometer case to increase heat transfer and use of “intelligent control” non-raster command signals, the frequency response of these galvanometers can be extended to exceed 5 kHz.
In one embodiment of a beam-steering device shown in
A reflective surface (not shown) being one of a variety of shapes (typically round or square) is attached (via its non-reflective side) to the support platform 104 where the center of the reflective surface is aligned to the center of the support platform 104. One galvanometer 101 rotates the support platform 104 and attached mirror to generate beam steering in the x-direction. The other galvanometer 102 rotates the same mirror platform 104 and attached mirror to generate beam steering in the y-direction. The combined movements of both galvanometers produce a tip-tilt motion of the mirror platform 104 capable of deflecting a beam simultaneously in both the x- and y-axes.
The shaft of each galvanometer 105 is attached via linkages 106 to the support platform 104. The linkage is stiff and noncompliant in the direction of rotation of the attached galvanometer, but permits movement in the orthogonal axis (i.e. in and out of the plane of the figure shown in
The actual position of the deflected beam in 2-dimensional space can be determined from reflector position feedback signals. During imaging applications, such signals are recorded concomitantly with intensity values obtained for light originating from the sample. Several options are available to generate such position signals. They can be obtained from galvanometer shaft position feedback signals typically available on the galvanometers. A more accurate (i.e. more closely aligned with actual beam direction) reflector position feedback signal can be obtained optically by detecting the position of light reflected off a surface of the reflector or by making capacitive measurements between the fixed platform 110 and the moveable reflector and its support platform 104. These options are illustrated schematically in
Another option is to measure capacitive changes between electrostatically-charged silicon comb-finger structures attached via linkages to the moveable support platform 104. This position-sensing scheme has been used by Milanovic et al. and is illustrated in
Should it be desired to generate more torque than can be supplied by a single galvanometer in order to further increase the frequency response of beam-steering devices, additional galvanometers can be placed in the same rotational axis on opposing sides of the mirror as shown in
The use of four galvanometric drives can be applied to most of the variations of the beam steering devices. The use of two galvanometers is a less expensive design if maximum frequency response is not necessary for particular applications. In some two-galvonometer designs, it is advantageous to stabilize at least one the sides of the reflector opposite to those attached to the galvanometers along the axis of the galvanometer linkages. This can be accomplished using identical linkages 106 to the galvanometer sides of the platform. Allowing these passive support linkages to rotate freely (with or without bearings) can stabilize the non-drive side(s) of the platform. The dashed lines in the vicinity of 218 (x-axis) and 217 (y-axis) in
A second general embodiment of a reflector platform and linkages is illustrated in
An example of a beam-steering design that can transfer rotational commands in x and y axes, while simultaneously allowing tip-tilt movements in both axes is shown in
The upper panel of
Finite element analysis is used to optimize the dimensions and chose the materials properties of the linkages for the size and mass of the reflective surface needed for particular applications.
The present invention was made with U.S. Government support under Agreement No. EPS0132556 awarded by the National Science Foundation. The U.S. Government has certain rights in this invention.
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/US05/07759 | 3/8/2005 | WO | 00 | 9/13/2007 |
Number | Date | Country | |
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60551655 | Mar 2004 | US |