Method and composition for treating osteoporosis

Abstract
This invention relates to a novel class of acidic amino acid/dicarboxylic acid derivatives (sulfonic acid/sulfate derivatives of naturally occurring amino acids and their amides) useful as inhibitors of osteoclastogenesis. The invention also provides methods of using the novel class of acidic amino acid/dicarboxylic acid derivatives of the general formula ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the compound is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2, for inhibition of osteoclastogenesis.
Description
FIELD OF THE INVENTION

This invention relates to a novel class of acidic amino acid/dicarboxylic acid derivatives (sulfonic acid/sulfate derivatives of naturally occurring amino acids and their amides) useful as inhibitors of osteoclastogenesis. More particularly, this invention relates to inhibitors of osteoclastogenesis containing novel class of acidic amino acid/dicarboxylic acid derivatives of the general formula: ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the compound is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2. Thus, the compounds may bear the general formula ZOC—CR7R8—CR5R6-CR3R4—CR1R2—COOH, ZOC—CR5R6—CR3R4—CR1R2—COOH or ZOC—CR3R4—CR1R2—COOH wherein Z is OH or NH2, and R1 to R8 denotes H, NH2, SO3H, or OSO3H, CH2—SO3H, CH2—OSO3H, NHSO3H.


A pharmaceutical composition containing the aforementioned inhibitors of osteoclastogenesis may also contain different divalent metal ions such as Mg, Ca or Zn. The composition consists of varying amounts of the above acidic amino acid/dicarboxylic acid derivatives and their pharmaceutically acceptable selected alkali/alkaline earth metal salts. The invention also provides a process for the preparation of the aforesaid compounds, useful for the inhibition of the osteoclast differentiation, maturation and activation. These compounds can also be used for developing effective drugs for the treatment of osteoporosis, osteoarthritis, bone metastasis and bone loss in other metabolic diseases of clinical importance.

    • 1) The inhibitors of osteoclastogenesis also contain different divalent metal ions such as Mg, Ca or Zn, wherein all the symbols are the same meaning as hereinafter defined and non-toxic salts thereof as an active ingredient,
    • 2) The composition consists of varying amounts of the above acidic amino acid/dicarboxylic acid derivatives and their pharmaceutically acceptable selected alkali/alkaline earth metal salts, wherein all the symbols are the same meaning as hereinafter defined and non-toxic salts thereof as an active ingredient,
    • 3) The process for the preparation of the aforesaid compounds, useful for the inhibition of the osteoclast differentiation, maturation and activation.
    • 4) These compounds can also be used for developing effective drugs for the treatment of osteoporosis, osteoarthritis, bone metastasis and bone loss in other metabolic diseases of clinical importance.


BACKGROUND OF THE INVENTION

Indian green mussels (Perna viridis) are the cheap source of proteins and considered as a delicacy. Extract prepared from green mussels by enzyme-acid hydrolysis process showed various biological activities. In our earlier patent (US patent application #20030044470) we have shown the inhibition of osteoclast differentiation and activation in the crude extract. In same continuation, attempts have been made to purify the active compound that showed inhibition of osteoclast differentiation and activation. The purification of the crude extract was done using HPLC, gel filtration and TLC methods. The purified compound was again checked for the above activity. The compound was characterized using NMR and LC-MS/MS techniques. This compound was synthesized and checked for the presence of the above biological activity. This patent in particular describes the synthesis of the compound and also its activity for inhibition of osteoclast formation.


Novel class of amino acid/dicarboxylic acid derivatives (sulfonic acid/sulfate derivatives of naturally occurring amino acids and their amides) along with calcium is for their activation to show inhibition of the osteoclastogenesis. Amino acid derivatives and calcium ion when used separately did not show any activity on inhibition of the osteoclastogenesis. The following classes of compounds are identified

    • (1) Natural acidic amino acids (Aspartic acid, Glutamic acid and their amides),
    • (2) Unnatural amino acids, amides such as homoglutamic acid,
    • (3) Dicarboxylic acids such as succinic acid, glutaric acid, and adipic acid
    • (4) N-sulfonyl, C-sulfonyl/sulfate derivatives of the above acids
    • (5) Alkaline earth metals such as Mg, Zn and Ca as their suitable salts.


      Related Arts


A lot of information is available on the matrix metalloproteinases (MMP's) commonly used as MMP inhibitors for the treatment of osteoporosis (Nigel, R. A. Beeley, Phillip, R. J., Ansell, Andrew, J. P., Dochert, 1994, Curr. Opin. Ther. Patents., 4, 7-16). A cylinder shaped solid compound has been prepared from the atelocollagen solution, L-alanine solution and bone morphogenetic protein for treating bone loss and elevating bone deformities (Hiroo, Akhihiko, Rebecca, Wozney, Seeherman, 2003, WO Patent #2003066083). In another study glutamate and glutamate derivatives/analogs or their mixtures have been used for modulating the bone quality (Tadeusz, Jose Luis; Stefan, 2003, WO Patent # 2003043626). Toshhiro (2003) invented a compound consisting of interacting trans-activators with glutamic acid, aspartic acid and rich carboxyl-terminal domain for estrogen receptor dependent activity (Toshihiro, 2002, WO Patent # 2003000730). Glutamic acid has been defined as an effective neuromediator and one of its derivatives is involved in osteoclast formation and bone resorption. The modification of glutamic acid action in bone could be used for bone remodeling (Hopital E. Herriot, Lyon Fr., 2002, Microscopy Research and technique, 58(2), 70-76).


However, these inhibitors have various problems and efforts were made for the development of non-peptide inhibitors. For instance in the specification of EP 606046, several aryl-sulfonamide derivatives have been described. In another invention several aryl sulfonyl amino acid derivatives of the following specifications have been described (Sakaki, Kanazawa, Sugiura, Miyazaki, Ohno, 2002, U.S. Pat. No. 6,403,644) as MMP inhibitors:

  • 1) N-[[4-(Benzoylamino)phenyl]sulfonyl]glycine,
  • 2) N-[[3-(Benzoylamino)phenyl]sulfonyl]glycine,
  • 3) N-[[2-(Benzoylamino)phenyl]sulfonyl]glycine,
  • 4) N-[[4-(Acetylamino)phenyl]sulfonyl]glycine,
  • 5) N-[[4-(Phenylacetylamino)phenyl]sulfonyl]glycine,
  • 6) N-[[4-[(Phenylethylcarbonyl)amino]phenyl]sulfonyl]glycine,
  • 7) N-[[4-(Cinnamoylamino)phenyl]sulfonyl]glycine,
  • 8) N-[[4-(N-Phenylureido)phenyl]sulfonyl]glycine,
  • 9) N-[[4-(N-Phenylthioureido)phenyl]sulfonyl]glycine,
  • 10) N-[[4-[(Benzyloxycarbonyl)amino]phenyl]sulfonyl]glycine,
  • 11) N-[[4-[(Phenyloxymethylcarbonyl)amino]phenyl]sulfonyl]glycine,
  • 12) N-[[4-[(Benzyloxymethylcarbonyl)amino]phenyl]sulfonyl]glycine,
  • 13) N-[[4-(4-Methoxybenzoylamino)phenyl]sulfonyl]glycine,
  • 14) N-[[4-(4-Fluorobenzoylamino)phenyl]sulfonyl]glycine,
  • 15) N-[[4-(4-Nitrobenzoylamino)phenyl]sulfonyl]glycine,
  • 16) N-[[4-(3-Nitrobenzoylamino)phenyl]sulfonyl]glycine,
  • 17) N-[[4-(2-Nitrobenzoylamino)phenyl]sulfonyl]glycine,
  • 18) N -[[4-(4-Formylbenzoylamino)phenyl]sulfonyl]glycine,
  • 19) N-[[4-(Benzoylamino)phenyl]sulfonyl]-D-alpha-phenylglycine,
  • 20) N-[[4-(Benzoylamino)phenyl]sulfonyl]-L-alpha-phenylglycine,
  • 21) N-[[4-(4-Methylbenzoylamino)phenyl]sulfonyl]-D-alpha-phenylglycine,
  • 22) N-[[4-(Methylbenzoylamino)phenyl]sulfonyl]-L-alpha-phenylglycine,
  • 23) N-[[4-(4-Methoxybenzoylamino)phenyl]sulfonyl]-D-alpha-phenylglycine,
  • 24) N-[[4-(4-Methoxybenzoylamino)phenyl]sulfonyl]-L-alpha-phenylglycine,
  • 25) N-[[4-(4-Fluorobenzoylamino)phenyl]sulfonyl]-D-alpha-phenylglycine,
  • 26) N-[[4-(4-Fluorobenzoylamino)phenyl]sulfonyl]-L-alpha-phenylglycine,
  • 27) N-[[4-(4-Nitrobenzoylamino)phenyl]sulfonyl]-D-alpha-phenylglycine,
  • 28) N-[[4-(4-Nitrobenzoylamino)phenyl]sulfonyl]-L-alpha-phenylglycine,
  • 29) N -[(4-Pivaloyloxyphenyl)sulfonyl]-D, L-alpha-phenylglycine,
  • 30) N-[(4-Pivaloyloxyphenyl)sulfonyl]-D, L-phenylalanine,
  • 31) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]glycine,
  • 32) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-alanine,
  • 33) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-beta-alanine,
  • 34) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-valine,
  • 35) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-valine,
  • 36) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-Ieucine,
  • 37) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-Ieucine,
  • 38) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-serine,
  • 39) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-phenylalanine,
  • 40) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-tyrosine,
  • 41) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-alanine methyl ester,
  • 42) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-valine methyl ester,
  • 43) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-valine methyl ester,
  • 44) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-Ieucine methyl ester,
  • 45) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-D, L-serine methyl ester,
  • 46) N-[[4-(2,4-Dichlorobenzoylamino)phenyl]sulfonyl]-L-tyrosine methyl ester,
  • 47) N-[[4-(3-Nitrobenzoylamino)phenyl]sulfonyl]-L-aspartic acid,
  • 48) N-[[3-(3-Nitrobenzoylamino)phenyl]sulfonyl]-L-aspartic acid,
  • 49) N-[[4-(3-Aminobenzoylamino)phenyl]sulfonyl]-L-aspartic acid,
  • 50) N-[[3-(3-Aminobenzoylamino)phenyl]sulfonyl]-L-aspartic acid,
  • 51) N-[[4-(Benzoylamino)phenyl]sulfonyl]-L-glutamic acid,
  • 52) N-[[4-(4-Chlorobenzoylamino)phenyl]sulfonyl]-L-glutamic acid,
  • 53) N-[[4-(4-Nitrobenzoylamino)phenyl]sulfonyl]-L-glutamic acid,
  • 54) N-[[4-[2-(4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-D,L-3-morpholinoalanine ethyl ester,
  • 55) N-[[4-[2-(4-(1-Nitrophenyl)butyryloxy]phenyl]sulfonyl]-D,L-3-morpholinoalanine ethyl ester,
  • 56) N-[[4-(2-Methoxy-2-phenylacetyloxy)phenyl]sulfonyl]-D,L-3-morpholinoalanine ethyl ester,
  • 57) N-[[4-[[[1-(4-Nitrophenyl)cyclobutyl]carbonyl]oxy]phenyl]sulfonyl]-D,L-3-morpholinoalanine ethyl ester,
  • 58) N-[[3-Methyl-4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-t-butoxycarbonyl-L-lysine,
  • 59) N-[[4-(2-Phenylbutyryloxy)phenyl]sulfonyl]glycine,
  • 60) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-D,L-phenylalanine,
  • 61) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-D, L-aspartic acid,
  • 62) N-[[4-[[[1-(4-Nitrophenyl)cyclobutyl]carbonyl]oxy]phenyl]sulfonyl]-D,L-aspartic acid,
  • 63)1-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonylamide]-I-cyclopropanecarboxylic acid,
  • 64) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-D,L-2-(2-furanyl)glycine,
  • 65) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-D,L-2-(2-tri-enyl)glycine,
  • 66) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-L-valine,
  • 67) N-[[4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-S-carboxymethyl-L-cysteine,
  • 68) N-[[4-[2-Ethyl-2-(4-methoxyphenyl)butyryloxy]phenyl]sulfonyl]-glycine,
  • 69) N-[[3-Methyl-4-[2-[4-(1-Pyrrolidinyl)phenyl]butyryloxy]phenyl]sulfonyl]-L-lysine,
  • 70) N-[[3 Methyl-4-[2-[4-(1-pyrrolidinyl)phenyl]butylyloxy]phenyl]sulfonyl]amino]pentanoic acid,
  • 71) N-[[(3-Methyl-4-pivaloyloxy)phenyl]sulfonyl]-beta-alanine.


    Purpose of the Invention


Bone is a metabolically active and highly organized connective tissue. The main functions of the bones are provision of mechanical and structural support, maintaining blood calcium levels, supporting haematopoiesis and housing the important vital organs such as brain, spinal cord and heart. To accomplish these functions bone needs continuous remodeling. Bone contains two distinct cell types, the osteoblasts, essential for bone formation (synthesis); and the osteoclasts, essential for bone resorption (break down). Morphogenesis and remodeling of bone involves the synthesis of bone matrix by osteoblasts and coordinated resorption by osteoclasts. The co-ordination between the osteoblasts and osteoclasts is very crucial in maintaining bone homeostasis and structural integrity of the skeleton. Both these processes are influenced by several hormones and local factors generated within bone and bone marrow, resulting in a complex network of control mechanisms. An imbalance of osteoblast and osteoclast functions can result in skeletal abnormalities characterized by increased or decreased bone mass. This may leads to excessive bone loss that reflects the balance of bone formation and bone resorption. Bone destruction or resorption is carried out by haematopoietically derived osteoclasts. Their number and activity is determined by cell lineage allocation, proliferation and differentiation of osteoclast precursors and the resorptive efficiency of mature osteoclasts. Important bone diseases such as osteoporosis, rheumatoid arthritis, Paget's disease of bone and bone metastasis of breast and prostate cancers are caused by increased osteoclast activity (Teitelbaum, 2000). In these disorders bone resorption exceeds bone formation resulting in decreased skeletal mass. This causes bones to become thin, fragile and susceptible to fracture. The consequences of osteoporotic bone fractures include chronic pain in bone, body deformity including height loss and muscle weakness. Therefore, to understand both pathogenesis and successful treatment of these bone diseases there is a need for better understanding of biology of osteoclasts.


Osteoporosis is now a serious problem that imposes substantial limitations on the affected individuals. In human, 1 in 3 women and 1 in 12 men over 45 years are at risk of suffering painful and deforming fractures as a result of osteoporosis. More women die after hip fractures than from cancers of ovaries, cervix and uterus. Osteoporosis occurs at a relatively earlier age in Indian males and females compared to western countries (Gupta, 1996). A variety of disadvantages are associated with current therapeutic agents used in osteoporosis and other metabolic bone disorders. The side effects of current therapies include increase in the risk of breast and uterine cancers, upper gastrointestinal distress and induction of immune responses. Drugs that inhibit the formation or activity of osteoclasts and with no toxicity and harmful side effects will be valuable for treating osteoporosis and other bone diseases.


Bone resorption and loss of calcium from bone are complications associated with arthritis, many cancers and with bone metastases of breast and prostate tumors. Because of lack of research into osteoporosis and related diseases, we don't know all the answers to treat these diseases. Progress in better understanding the pathogenesis and successful treatment of these diseases to date has targeted osteoclast.


Osteoclasts, the only cells capable of resorbing bone differentiate from the haemopoietic precursors of monocyte/macrophage lineage that also give rise to macrophages and dendritic cells (Miyamoto et al. 2001). Lineage choice and the differentiation process is guided by lineage restricted key regulatory molecules and transcription factors. Osteoclasts are large multinucleated cells. They are formed by the fusion of mononuclear cells of haemopoietic origin and not by mitosis, since DNA synthesis is not required. Osteoclast formation and bone resorption is regulated by many hormones, growth factors and immune cell-derived cytokines (Udagawa et al. 1995, Wani et al. 1999, Fox et al. 2000, Fuller et al. 2000). These factors act directly or indirectly via other cell types to influence osteoclast differentiation. The most important cell type influencing osteoclast formation is osteoblast, which promote this process by a contact-dependent mechanism. Recent advances revealed that two molecules, macrophage colony stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL) expressed by osteoblasts are essential and sufficient for the differentiation of haemopoietic cells to form osteoclasts (Tanaka et al. 1993, Anderson et al., 1997, Wong et al. 1997, Lacey et al. 1998, Yasuda et al. 1998). The precise role of other cells, such as T lymphocytes in bone homeostasis is yet to be fully elucidated. It has recently been reported that activated T cells regulates osteoclast formation by some unknown mechanisms. T cells support osteoclast formation by RANKL-dependent and RANKL-independent mechanisms (Weitzmann et al. 2001). Cytokines produced by activated T cells, as well as by other cell types regulates osteoclastogenesis in physiological and pathological conditions. Recent discovery of RANKL has enabled us for the meticulous dissection of mechanisms by which various factors regulate osteoclastogenesis, and better understanding of both pathogenesis and successful treatment bone diseases. In our preliminary studies, we have investigated the role of novel compounds on osteoclastogenesis induced by RANKL in the presence of M-CSF in stromal cell-free cultures of osteoclast precursors.


Natural products from plants and organisms have frequently been used as a source for development of effective drugs. There is an increased interest in analysis of natural products from marine organisms. Sea animals contain metabolites which can be used for treatment of many diseases.


The inventors have previously shown (U.S. Pat. No. 2,003,066083) that a novel extract (mussel hydrolysate) prepared from the Indian green mussel (Perna viridis) inhibits the osteoclast differentiation in murine haemopoietic precursors of monocyte/macrophage cell lineage. The extract also inhibits the bone resorbing activity of osteoclasts. There was approximately 80-90% inhibition of osteoclast formation and bone resorption in the presence of extract. More importantly, the extract is non-toxic to other cells and is useful to prepare a drug for the treatment of osteoporosis and other bone diseases.


In further investigation, at each stages of purification we found a significant inhibition of osteoclast formation and bone resorption (60-90%). We have purified some active components from extract and these active components significantly inhibit both osteoclast formation and bone resorption. These active compounds can be used in therapeutic settings to protect and cure the individuals against osteoporosis and other metabolic bone diseases.


The current known therapeutic agents have a variety of associated disadvantages. The side effects of current therapies include an elevated risk of breast and uterine cancers, upper gastrointestinal distress and induction of immune responses (Watts' 1999). Our earlier US Patent (#2003066083) describes preparation of mussell hydrolysate from the Indian green mussel (Perna viridis) and its inhibition of the osteoclast differentiation in murine hemopoietic precursors of monocyte/macrophage cell lineage. The extract also shows inhibition of the bone resorbing activity of osteoclasts.


The present inventors have found that a series of novel sulfonic acid/sulfate derivatives of acidic amino acids, aspartic acids, glutamic acid, homoglutamic acid and their related aliphatic dicarboxylic acids (Succinic acid, glutaric acid and adipic acid) have inhibitory activity against osteoclast formation and bone resorption. These compounds are novel and non-toxic to other cells. The active compounds may play a vital role in inhibition of differentiation of osteoclast from hemopoietic precursors and can be used in therapeutic settings to protect and cure the individuals against osteoporosis and other metabolic bone diseases.


SUMMARY OF THE INVENTION

This invention relates to a novel class of acidic amino acid/dicarboxylic acid derivatives (sulfonic acid/sulfate derivatives of naturally occurring amino acids and their amides) useful as inhibitors of osteoclastogenesis. The invention also provides methods of using the novel class of acidic amino acid/dicarboxylic acid derivatives of the general formula ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the composition is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2. Thus, the compounds may bear the general formula ZOC—CR7R8—CR5R6—CR3R4—CR1R2—COOH, ZOC—CR5R6—CR3R4—CR1R2—COOH or ZOC—CR3R4—CR1R2—COOH wherein Z is OH or NH2, and R1 to R8 denotes H, NH2, SO3H, or OSO3H, CH2—SO3H, CH2—OSO3H, NHSO3H. Mixtures of these compounds may be administered, as well.







DETAILED DESCRIPTION OF THE INVENTION

The present invention is related to:

  • A) Osteoclast inhibitors containing novel class of acidic amino acid/dicarboxylic acid derivatives (sulfonic acid/sulfate derivatives of naturally occurring amino acids and their amides);
  • B) Osteoclast inhibitors containing novel class of acidic amino acid/dicarboxylic acid derivatives of the general formula ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the composition is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2. Thus, compounds may bear the general formula:

    ZOC—CR3R4—CR1R2—COOH  (Ia)
    ZOC—CR5R6—CR3R4—CR1R2—COOH  (Ib)
    ZOC—CR7R8—CR5R6—CR3R4—CR1R2—COOH  (Ic)

    wherein:


Z is OH or NH2; and


R1 to R8 are H, NH2, SO3H or OSO3H, CH2—SO3H, CH2—OSO3H, NHSO3H. And this includes the following class of compounds:

    • 1. A compound in which Z═OH, R1═NHSO3H, R2═R3═R4═H is the same meaning as hereinbefore defined;
    • 2. A compound in which Z═OH, R1═NH2, R3═R4═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 3. A compound in which Z═OH, R1═NH2, R3═R4═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 4. A compound in which Z═OH, R1═NH2, R2═R4═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 5. A compound in which Z═OH, R1═NH2, R2═R4═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 6. A compound in which Z═OH, R1═NH2, R2═R3═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 7. A compound in which Z═OH, R1═NH2, R2═R3═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 8. A compound in which Z═OH, R1═R3═R4═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 9. A compound in which Z═OH, R1═R3═R4═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 10. A compound in which Z═OH, R1═R3═R4═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 11. A compound in which Z═OH, R1═R3═R4═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 12. A compound in which Z═OH, R2═NHSO3H, R1═R3═R4═H is the same meaning as hereinbefore defined;
    • 13. A compound in which Z═OH, R2═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 14. A compound in which Z═OH, R2═H, R1═CH2OSO3H is the same meaning as hereinbefore defined;
    • 15. A compound in which Z═OH, R2═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 16. A compound in which Z═OH, R2═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 17. A compound in which Z═OH, R2═NH2, R3═R4═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 18. A compound in which Z═OH, R2═NH2, R3═R4═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 19. A compound in which Z═OH, R2═NH2, R1═R4═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 20. A compound in which Z═OH, R2═NH2, R═R4═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 21. A compound in which Z═OH, R2═NH2, R1═R3═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 22. A compound in which Z═OH, R2═NH2, R1═R3═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 23. A compound in which R1═NHSO3H, R2═R3═R4═H is the same meaning as hereinbefore defined;
    • 24. A compound in which Z═NH2, R1═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 25. A compound in which Z═NH2, R1═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 26. A compound in which Z═NH2, R1═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 27. A compound in which Z═NH2, R1═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 28. A compound in which Z═R1═NH2, R2═R4═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 29. A compound in which Z═R1═NH2, R2═R4═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 30. A compound in which Z═R1═NH2, R2═R4═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 31. A compound in which Z═R1═NH2, R2═R4═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 32. A compound in which Z═R1═NH2, R2═R3═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 33. A compound in which Z═R1═NH2, R2═R3═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 34. A compound in which Z═NH2, R2═NHSO3H, R1═R3═R4═H is the same meaning as hereinbefore defined;
    • 35. A compound in which Z═NH2, R2 to R4═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 36. A compound in which Z═NH2, R2 to R4═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 37. A compound in which Z═OH, R2 to R4═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 38. A compound in which Z═OH, R2 to R4═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 39. A compound in which Z═R2═NH2, R3═R4═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 40. A compound in which Z═R2═NH2, R3═R4═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 41. A compound in which Z═R2═NH2, R1═R4═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 42. A compound in which Z═R2═NH2, R1═R4═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 43. A compound in which Z═R2═NH2, R1═R3═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 44. A compound in which Z═R2═NH2, R1═R3═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 45. A compound in which Z═OH, R1═NHSO3H, R2═R3═R4═R5═R6═H is the same meaning as hereinbefore defined;
    • 46. A compound in which Z═OH, R1, R3 to R6═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 47. A compound in which Z═OH, R1, R3 to R6═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 48. A compound in which Z═OH, R1, R3 to R6═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 49. A compound in which Z═OH, R1, R3 to R6═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 50. A compound in which Z═OH, R2 to R6═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 51. A compound in which Z═OH, R2 to R6═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 52. A compound in which Z═OH, R1═NH2, R3 to R6═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 53. A compound in which Z═OH, R1═NH2, R3 to &═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 54. A compound in which Z═OH, R1═NH2, R2═H, R4 to R6═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 55. A compound in which Z═OH, R1═NH2, R2═H, R4 to R6═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 56. A compound in which Z═OH, R1═NH2, R2═R3═R5═R6═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 57. A compound in which Z═OH, R1═NH2, R2═R3═R5═R6═H, R4═OSO3H is same meaning as hereinbefore defined;
    • 58. A compound in which Z═OH, R1═NH2, R2═R3═R4═R6═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 59. A compound in which Z═OH, R1═NH2, R2═R3═R4═R6═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 60. A compound in which Z═OH, R1═NH2, R2 to R5═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 61. A compound in which Z═OH, R1═NH2, R2 to R5═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 62. A compound in which Z═OH, R2═NHSO3H, R1, R3 to R6═H is the same meaning as hereinbefore defined;
    • 63. A compound in which Z═OH, R2 to R6═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 64. A compound in which Z═OH, R2 to R6═H, R1═CH2OSO3H is the same meaning as hereinbefore defined;
    • 65. A compound in which Z═OH, R2═NH2, R3 to R6H, R1═SO3H is the same meaning as hereinbefore defined;
    • 66. A compound in which Z═OH, R2═NH2, R3 to R6H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 67. A compound in which Z═OH, R2═NH2, R1, R4 to R6H, R3═SO3H is the same meaning as hereinbefore defined;
    • 68. A compound in which Z═OH, R2═NH2, R1, R4 to R6H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 69. A compound in which Z═OH, R2═NH2, R1═R3═R5═R6═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 70. A compound in which Z═OH, R2═NH2, R1═R3═R5═R6═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 71. A compound in which Z═OH, R2═NH2, R1═R3═R4═R6═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 72. A compound in which Z═OH, R2═NH2, R1═R3═R4═R6═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 73. A compound in which Z═OH, R2═NH2, R1═R3═R4═R5═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 74. A compound in which Z═OH, R2═NH2, R1═R3═R4═R5═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 75. A compound in which Z═NH2, R1═NHSO3H, R2 to R6═H is the same meaning as hereinbefore defined;
    • 76. A compound in which Z═R1═NH2, R3 to R6═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 77. A compound in which Z═R1═NH2, R3 to R6═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 78. A compound in which Z═R1═NH2, R2═H, R3 to R6═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 79. A compound in which Z═R1═NH2, R2═H, R3 to R6═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 80. A compound in which Z═R1═NH2, R2═R3═R5═R6═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 81. A compound in which Z═R1═NH2, R2═R3═R5═R6═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 82. A compound in which Z═R1═NH2, R2═R3═R4═R6═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 83. A compound in which Z═R1═NH2, R2═R3═R4═R6═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 84. A compound in which Z═R1═NH2, R2 to R5═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 85. A compound in which Z═R1═NH2, R2 to R5═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 86. A compound in which Z═NH2, R1, R3 to R6═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 87. A compound in which Z═NH2, R1, R3 to R6═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 88. A compound in which Z═NH2, R1, R3 to R6═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 89. A compound in which Z═NH2, R1, R3 to R6═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 90. A compound in which Z═NH2, R2 to R6═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 91. A compound in which Z═NH2, R2 to R6═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 92. A compound in which Z═NH2, R2═NHSO3H; R1═H, R3 to R6═H is the same meaning as hereinbefore defined;
    • 93. A compound in which Z═NH2, R2 to R6═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 94. A compound in which Z═NH2, R2 to R6═H, R1═CH2OSO3H is the same meaning as hereinbefore defined;
    • 95. A compound in which Z═NH2, R2═NH2, R3 to R6═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 96. A compound in which Z═NH2, R2═NH2, R3 to R6═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 97. A compound in which Z═NH2, R2═NH2, R1, R4 to R6H, R3═SO3H is the same meaning as hereinbefore defined;
    • 98. A compound in which Z═R2═NH2, R1, R4 to & H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 99. A compound in which Z═R2═NH2, R1═R3═R5═R6═H, R4═SO3H is the same meaning as hereinbefore defined;
  • 100. A compound in which Z═R2═NH2, R1═R3═R5═R6═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 101. A compound in which Z═R2═NH2, R1═R3═R4═R6═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 102. A compound in which Z═R2═NH2, R1═R3═R4═R6═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 103. A compound in which Z═R2═NH2, R1═R3═R4═R5═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 104. A compound in which Z═R2═NH2, R1═R3═R4═R5═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 105. A compound in which Z═OH, R1═NHSO3H, R2 to R8═H is the same meaning as hereinbefore defined;
    • 106. A compound in which Z═OH, R1, R3 to R8═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 107. A compound in which Z═OH, R1, R3 to R8═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 108. A compound in which Z═OH, R1, R3 to R8═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 109. A compound in which Z═OH, R1, R3 to R8═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 110. A compound in which Z═OH, R1═NH2, R3 to R8═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 111. A compound in which Z═OH, R1═NH2, R3 to R8═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 112. A compound in which Z═OH, R1═NH2, R2═H, R4 to R8═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 113. A compound in which Z═OH, R1═NH2, R2═H, R4 to R8═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 114. A compound in which Z═OH, R1═NH2, R2═R3═H, R5 to R8═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 115. A compound in which Z═OH, R1═NH2, R2═R3═H, R5 to R8═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 116. A compound in which Z═OH, R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 117. A compound in which Z═OH, R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 118. A compound in which Z═OH, R1 ═N—H2, R2═R5═H, R7═R8═H, R6═SO3H the same meaning as hereinbefore defined;
    • 119. A compound in which Z═OH, R1═NH2, R2═R5═H, R7═R8═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 120. A compound in which Z═OH, R1═NH2, R2 to R6═H, R8═H, R7═SO3H is the same meaning as is before defined;
    • 121. A compound in which Z═OH, R1═NH2, R2 to R6═H, R8═H, R7═OSO3H is the same meaning as hereinbefore defined;
    • 122. A compound in which Z═OH, R1═NH2, R2 to R7═H, R8═SO3H is the same meaning as hereinbefore defined;
    • 123. A compound in which Z═OH, R1═NH2, R2 to R7═H, R8═OSO3H is the same meaning as hereinbefore defined;
    • 124. A compound in which Z═OH, R2═NHSO3H, R1, R3 to R8═H is the same meaning as hereinbefore defined;
    • 125. A compound in which Z═OH, R2 to R8═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 126. A compound in which Z═OH, R2 to R8═H, R1═CH2OSO3H is the same meaning as hereinbefore defined;
    • 127. A compound in which Z═OH, R2 to R8═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 128. A compound in which Z═OH, R2 to R8═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 129. A compound in which Z═OH, R2═NH2, R3 to R8═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 130. A compound in which Z═OH, R2═NH2, R3 to R8═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 131. A compound in which Z═OH, R2═NH2, R1, R4 to R8═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 132. A compound in which Z═OH, R2═NH2, R1, R4 to R8═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 133. A compound in which Z═OH, R2═NH2, R1═R3═H, R5 to R8═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 134. A compound in which Z═OH, R2═NH2, R1═R3═H, R5 to R8═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 135. A compound in which Z═OH, R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═SO3H is the same meaning as is before defined;
    • 136. A compound in which Z═OH, R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 137. A compound in which Z═OH, R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 138. A compound in which Z═OH, R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 139. A compound in which Z═OH, R2═NH2, R1═R8═H, R3 to R6═H, R7═SO3H the same meaning as hereinbefore defined;
    • 140. A compound in which Z═OH, R2═NH2, R1═R8═H, R3 to R6═H, R7═OSO3 is the same meaning as hereinbefore defined;
    • 141. A compound in which Z═OH, R2═NH2, R1═H, R3 to R7═H, R8═SO3H is the same meaning as hereinbefore defined;
    • 142. A compound in which Z═OH, R2═NH2, R1═H, R3 to R7═H, R8═OSO3H is the same meaning as hereinbefore defined;
    • 143. A compound in which Z═NH2, R1═NHSO3H, R2 to R8═H is the same meaning as hereinbefore defined;
    • 144. A compound in which Z═NH2, R1 and R3 to R8═H, R2═CH2SO3H is the same meaning as hereinbefore defined;
    • 145. A compound in which Z═NH2, R1 and R3 to R8═H, R2═CH2OSO3H is the same meaning as hereinbefore defined;
    • 146. A compound in which Z═NH2, R1 and R3 to R8═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 147. A compound in which Z═NH2, R1 and R3 to R8═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 148. A compound in which Z═R1═NH2, R3 to R8═H, R2═SO3H is the same meaning as hereinbefore defined;
    • 149. A compound in which Z═R1═NH2, R3 to R8═H, R2═OSO3H is the same meaning as hereinbefore defined;
    • 150. A compound in which Z═R1═NH2, R2═H, R4 to R8═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 151. A compound in which Z═R1═NH2, R2═H, R4 to R8═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 152. A compound in which Z═R1═NH2, R2═R3═H, R5 to R8═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 153. A compound in which Z═R1═NH2, R2═R3═H, R5 to R8═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 154. A compound in which Z═R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 155. A compound in which Z═R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 156. A compound in which Z═R1═NH2, R2═R5═H, R7═R8═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 157. A compound in which Z═R1═NH2, R2═R5═H, R7═R8═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 158. A compound in which Z═R1═NH2, R2 to R6═H, R8═H, R7═SO3H is the same meaning as hereinbefore defined;
    • 159. A compound in which Z═R1═NH2, R2 to R6═H, R8═H, R7═OSO3H is the same meaning as hereinbefore defined;
    • 160. A compound in which Z═R1═NH2, R2 to R7═H, R8═SO3H is the same meaning as hereinbefore defined;
    • 161. A compound in which Z═R1═NH2, R2 to R7═H, R8═OSO3H is the same meaning as hereinbefore defined;
    • 162. A compound in which Z═NH2, R2═NHSO3H, R1 and R3 to R8═H is the same meaning as hereinbefore defined;
    • 163. A compound in which Z═NH2, R2 to R8═H, R1═CH2SO3H is the same meaning as hereinbefore defined;
    • 164. A compound in which Z═NH2, R2 to R8═H, R1═CH2OSO3H is the same meaning as hereinbefore defined;
    • 165. A compound in which Z═NH2, R2 to R8═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 166. A compound in which Z═NH2, R2 to R8═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 167. A compound in which Z═R2═NH2, R3 to R8═H, R1═SO3H is the same meaning as hereinbefore defined;
    • 168. A compound in which Z═R2═NH2, R3 to R8═H, R1═OSO3H is the same meaning as hereinbefore defined;
    • 169. A compound in which Z═R2═NH2, R1, R4 to R8═H, R3═SO3H is the same meaning as hereinbefore defined;
    • 170. A compound in which Z═R2═NH2, R1, R4 to R8═H, R3═OSO3H is the same meaning as hereinbefore defined;
    • 171. A compound in which Z═R2═NH2, R1═R3═H, R5 to R8═H, R4═SO3H is the same meaning as hereinbefore defined;
    • 172. A compound in which Z═R2═NH2, R1═R3═H, R5 to R8═H, R4═OSO3H is the same meaning as hereinbefore defined;
    • 173. A compound in which Z═R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═SO3H is the same meaning as hereinbefore defined;
    • 174. A compound in which Z═R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═OSO3H is the same meaning as hereinbefore defined;
    • 175. A compound in which Z═R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═SO3H is the same meaning as hereinbefore defined;
    • 176. A compound in which Z═R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═OSO3H is the same meaning as hereinbefore defined;
    • 177. A compound in which Z═R2═NH2, R1═R8═H, R3 to R6═H, R7═SO3H is the same meaning as hereinbefore defined;
    • 178. A compound in which Z═R2═NH2, R1═R8═H, R3 to R6═H, R7═OSO3H is the same meaning as hereinbefore defined;
    • 179. A compound in which Z═R2═NH2, R1═H, R3 to R7═H, R8═SO3H is the same meaning as hereinbefore defined;
    • 180. A compound in which Z═R2═NH2, R1═H, R3 to R7═H, R8═OSO3H is the same meaning as hereinbefore defined.


      C) The osteoclast inhibitors also contained different divalent metal ions such as Mg, Ca and Zn. The composition consisted of varying amounts of the above acid amino acid/dicarboxylic acid derivatives and their pharmaceutically acceptable salts. Non toxic salts of the present invention are contained all pharmaceutically acceptable salts, for example, general salts, acid addition salt, hydrate salts.


The compounds of the formulae (Ia), (lb) and (Ic) of the present invention may be converted into the corresponding salts. Non toxic and water soluble salts are preferable. Suitable salts for example are as follows:

    • Salts of alkaline earth metals (Mg, Ca etc)
    • Ammonium Salts
    • Salts of pharmaceutically acceptable organic amines (tetramethyl ammonium, triethyl amine, methyl amine, cyclopentyl amine, benzylamine, phenethylamine, piperidine, monoethanolamine, diethanolamine, tris(hydroxymethyl)amine, lysine, arginine, N-methyl glucamine, etc.


      d) In the compound of the present invention of the formulae (Ia), (lb) and (Ic) the following non toxic derivatives thereof are preferable:
    • 1. L-Aspartic acid, N-Sulfonic acid
    • 2. L-Aspartic acid, 2β-sulfonic acid
    • 3. L-Aspartic acid, 2β-sulfate
    • 4. L-aspartic acid, 3α-sulfonic acid
    • 5. L-aspartic acid, 3α-sulfate
    • 6. L-aspartic acid, 3β-sulfonic acid
    • 7. L-aspartic acid, 3β-sulfate
    • 8. 2α,3-dicarboxy, propane-1-sulfonic acid
    • 9. 2α,3-dicarboxy, propane-1-sulfate
    • 10. 1α,2-carboxy ethane sulfonic acid
    • 11. 1α,2-carboxy ethane sulfate
    • 12. D-aspartic acid, N-sulfonic acid
    • 13. 2β,3-carboxy, propane-1-sulfonic acid
    • 14. 2β,3-carboxy, propane-1-sulfate
    • 15. 1β,2-carboxy ethane-1-sulfonic acid
    • 16. 1β,2-carboxy ethane-1-sulfate
    • 17. D-aspartic acid, 2α-sulfonic acid
    • 18. D-aspartic acid, 2α-sulfonic acid
    • 19. D-Aspartic acid, 3α-sulfonic acid
    • 20. D-Aspartic acid, 3α-sulfate
    • 21. D-Aspartic acid, 3β-sulfonic acid
    • 22. D-aspartic acid, 3β-sulfate
    • 23. L-asparagine, N-sulfonic acid
    • 24. 2α-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 25. 2α-carboxy, 3-carboxamido, propane-1-sulfate
    • 26. 1α-carboxy, 2-carboxamido, ethane sulfonic acid
    • 27. 1α-carboxy, 2-carboxamido, ethane sulfate
    • 28. L-asparagine, 2β-sulfonic acid
    • 29. -asparagine, 2β-sulfate
    • 30. L-asparagine, 3α-sulfonic acid
    • 31. L-asparagine, 3α-sulfate
    • 32. L-asparagine, 3β-sulfonic acid
    • 33. L-asparagine, 3β-sulfate
    • 34. D-asparagine, N-sulfonic acid
    • 35. 2β-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 36. 2β-carboxy, 3-carboxamido, propane-1-sulfate
    • 37. 1β-carboxy, 2-carboxamido, ethane sulfonic acid
    • 38. 1β-carboxy, 2-carboxamido, ethane sulfate
    • 39. D-asparagine, 2α-sulfonic acid
    • 40. D-asparagine, 2α-sulfate
    • 41. D-asparagine, 3α-sulfonic acid
    • 42. D-asparagine, 3α-sulfate
    • 43. D-asparagine, 3β-sulfonic acid
    • 44. D-asparagine, 3β-sulfate
    • 45. L-glutamic acid, N-sulfonic acid
    • 46. 2α,4-dicarboxy, butane-1-sulfonic acid
    • 47. 2α,4-dicarboxy, butane-1-sulfate
    • 48. 1α,3-dicarboxy, propane sulfonic acid
    • 49. 1α,3-dicarboxy, propane sulfate
    • 50. 1β,3-dicarboxy, propane sulfate
    • 51. 1β,3-dicarboxy, propane sulfonic acid
    • 52. L-glutamic acid, 2β-sulfonic acid
    • 53. L-glutamic acid, 2β-sulfate
    • 54. L-glutamic acid, 3α-sulfonic acid
    • 55. L-glutamic acid, 3α-sulfate
    • 56. L-glutamic acid, 3β-sulfonic acid
    • 57. L-glutamic acid, 3β-sulfate
    • 58. L-glutamic acid, 4α-sulfonic acid
    • 59. L-glutamic acid, 4α-sulfate
    • 60. L-glutamic acid, 4β-sulfonic acid
    • 61. L-glutamic acid, 4β-sulfate
    • 62. D-glutamic acid, N-sulfonic acid
    • 63. 2β,4-dicarboxy, butane-1-sulfonic acid
    • 64. 2β,4-dicarboxy, butane-1-sulfate
    • 65. D-glutamic acid, 2α-sulfonic acid
    • 66. D-glutamic acid, 2α-sulfate
    • 67. D-glutamic acid, 3α-sulfonic acid
    • 68. D-glutamic acid, 3α-sulfate
    • 69. D-glutamic acid, 3β-sulfonic acid
    • 70. D-glutamic acid, 3β-sulfate
    • 71. D-glutamic acid, 4α-sulfonic acid
    • 72. D-glutamic acid, 4α-sulfate
    • 73. D-glutamic acid, 4β-sulfonic acid
    • 74. D-glutamic acid, 4β-sulfate
    • 75. L-glutamine, N-sulfonic acid
    • 76. L-glutamine, 2β-sulfonic acid
    • 77. L-glutamine, 2β-sulfate
    • 78. L-glutamine, 3α-sulfonic acid
    • 79. L-glutamine, 3α-sulfate
    • 80. L-glutamine, 3β-sulfonic acid
    • 81. L-glutamine, 3β-sulfate
    • 82. L-glutamine, 4α-sulfonic acid
    • 83. L-glutamine, 4α-sulfate
    • 84. L-glutamine, 4β-sulfonic acid
    • 85. L-glutamine, 4β-sulfate
    • 86. 2α-carboxy, 4-carboxamido, butane-1-sulfonic acid
    • 87. 2α-carboxy, 4-carboxamido, butane-1-sulfate
    • 88. 1α-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 89. 1α-carboxy, 3-carboxamido, propane-1-sulfate
    • 90. 1β-carboxy, 3-carboxamido, propane-1-sulfate
    • 91. 1β-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 92. D-glutamine, N-sulfonic acid
    • 93. 2β-carboxy, 4-carboxamido, butane-1-sulfonic acid
    • 94. 2β-carboxy, 4-carboxamido, butane-1-sulfate
    • 95. D-glutamine, 2α-sulfonic acid
    • 96. D-glutamine, 2α-sulfate
    • 97. D-glutamine, 3α-sulfonic acid
    • 98. D-glutamine, 3α-sulfate
    • 99. D-glutamine, 3β-sulfonic acid
    • 100. D-glutamine, 3β-sulfate
    • 101. D-glutamine, 4α-sulfonic acid
    • 102. D-glutamine, 4α-sulfate
    • 103. D-glutamine, 4β-sulfonic acid
    • 104. D-glutamine, 4β-sulfate
    • 105. L-homoglutamic acid, N-sulfonic acid
    • 106. Pentane-2α,5-dicarboxy-1-sulfonic acid
    • 107. Pentane-2α,5-dicarboxy-1-sulfate
    • 108. Butane-1α,4-dicarboxy-1-sulfonic acid
    • 109. Butane-1α,4-dicarboxy-1-sulfate
    • 110. L-homoglutamic acid, 2β-sulfonic acid
    • 111. L-homoglutamic acid, 2β-sulfate
    • 112. L-homoglutamic acid, 3α-sulfonic acid
    • 113. L-homoglutamic acid, 3α-sulfate
    • 114. L-homoglutamic acid, 3β-sulfonic acid
    • 115. L-homoglutamic acid, 3β-sulfate
    • 116. L-homoglutamic acid, 4α-sulfonic acid
    • 117. L-homoglutamic acid, 4α-sulfate
    • 118. L-homoglutamic acid, 4β-sulfonic acid
    • 119. L-homoglutamic acid, 4β-sulfate
    • 120. L-homoglutamic acid, 5α-sulfonic acid
    • 121. L-homoglutamic acid, 5α-sulfate
    • 122. L-homoglutamic acid, 5β-sulfonic acid
    • 123. L-homoglutamic acid, 5β-sulfate
    • 124. D-homoglutamic acid, N-sulfonic acid
    • 125. Pentane-2β, 5-dicarboxy-1-sulfonic acid
    • 126. Pentane-2β, 5-dicarboxy-1-sulfate
    • 127. Butane-1β, 4-dicarboxy-1-sulfonic acid
    • 128. Butane-1β, 4-dicarboxy-1-sulfate
    • 129. D-homoglutamic acid, 2α-sulfonic acid
    • 130. D-homoglutamic acid, 2α-sulfate
    • 131. D-homoglutamic acid, 3α-sulfonic acid
    • 132. D-homoglutamic acid, 3α-sulfate
    • 133. D-homoglutamic acid, 3β-sulfonic acid
    • 134. D-homoglutamic acid, 3β-sulfate
    • 135. D-homoglutamic acid, 4α-sulfonic acid
    • 136. D-homoglutamic acid, 4α-sulfate
    • 137. D-homoglutamic acid, 4β-sulfonic acid
    • 138. D-homoglutamic acid, 4β-sulfate
    • 139. D-homoglutamic acid, 5α-sulfonic acid
    • 140. D-homoglutamic acid, 5α-sulfate
    • 141. D-homoglutamic acid, 5β-sulfonic acid
    • 142. D-homoglutamic acid, 5β-sulfate
    • 143. L-homoglutamine, N-sulfonic acid
    • 144. Pentane-2α-carboxy, 5-carboxamido-1-sulfonic acid
    • 145. Pentane-2α-carboxy, 5-carboxamido-1-sulfate
    • 146. Butane-1α-carboxy, 4-carboxamido-1-sulfonic acid
    • 147. Butane-1α-carboxy, 4-carboxamido-1-sulfate
    • 148. L-homoglutamine, 2β-sulfonic acid
    • 149. L-homoglutamine, 2β-sulfate
    • 150. L-homoglutamine, 3α-sulfonic acid
    • 151. L-homoglutamine, 3α-sulfate
    • 152. L-homoglutamine, 3β-sulfonic acid
    • 153. L-homoglutamine, 3β-sulfate
    • 154. L-homoglutamine, 4α-sulfonic acid
    • 155. L-homoglutamine, 4α-sulfate
    • 156. L-homoglutamine, 4β-sulfonic acid
    • 157. L-homoglutamine, 4β-sulfate
    • 158. L-homoglutamine, 5α-sulfonic acid
    • 159. L-homoglutamine, 5α-sulfate
    • 160. L-homoglutamine, 5β-sulfonic acid
    • 161. L-homoglutamine, 5β-sulfate
    • 162. D-homoglutamine, N-sulfonic acid
    • 163. Pentane-2β-carboxy, 5-carboxamido-1-sulfonic acid
    • 164. Pentane-2β-carboxy, 5-carboxamido-1-sulfate
    • 165. Butane-1β-carboxy, 4-carboxamido-1-sulfonic acid
    • 166. Butane-1β-carboxy, 4-carboxamido-1-sulfate
    • 167. D-homoglutamine, 2α-sulfonic acid
    • 168. D-homoglutamine, 2α-sulfate
    • 169. D-homoglutamine, 3α-sulfonic acid
    • 170. D-homoglutamine, 3α-sulfate
    • 171. D-homoglutamine, 3β-sulfonic acid
    • 172. D-homoglutamine, 3β-sulfate
    • 173. D-homoglutamine, 4α-sulfonic acid
    • 174. D-homoglutamine, 4α-sulfate
    • 175. D-homoglutamine, 4β-sulfonic acid
    • 176. D-homoglutamine, 4β-sulfate
    • 177. D-homoglutamine, 5α-sulfonic acid
    • 178. D-homoglutamine, 5α-sulfate
    • 179. D-homoglutamine, 5β-sulfonic acid
    • 180. D-homoglutamine, 5β-sulfate.


      e) a process for the preparation of sulfonic acid/sulfate derivatives of the formula (Ia) and non-toxic salts thereof:
    • 1. A compound wherein Z═OH, R1═NHSO3H, R2═R3═R4═H;
    • 2. A compound wherein Z═OH, R1═NH2, R3═R4═H, R2═SO3H;
    • 3. A compound in which Z═OH, R1═NH2, R3═R4═H, R2═OSO3H;
    • 4. A compound in which Z═OH, R1═NH2, R2═R4═H, R3═SO3H;
    • 5. A compound in which Z═OH, R1═NH2, R2═R4═H, R3═OSO3H;
    • 6. A compound in which Z═OH, R1═NH2, R2═R3═H, R4═SO3H;
    • 7. A compound in which Z═OH, R1═NH2, R2═R3═H, R4═OSO3H;
    • 8. A compound in which Z═OH, R1═R3═R4═H, R2═CH2SO3H;
    • 9. A compound in which Z═OH, R1═R3═R4═H, R2═CH2OSO3H;
    • 10. A compound in which Z═OH, R1═R3═R4═H, R2═SO3H;
    • 11. A compound in which Z═OH, R1═R3═R4═H, R2═OSO3H;
    • 12. A compound in which Z═OH, R2═NHSO3H, R1═R3═R4═H;
    • 13. A compound in which Z═OH, R2═H, R1═CH2SO3H;
    • 14. A compound in which Z═OH, R2═H, R1═CH2OSO3H;
    • 15. A compound in which Z═OH, R2═H, R1═SO3H;
    • 16. A compound in which Z═OH, R2═H, R1═OSO3H;
    • 17. A compound in which Z═OH, R2═NH2, R3═R4═H, R1═SO3H;
    • 18. A compound in which Z═OH, R2═NH2, R3═R4═H, R1═SO3H;
    • 19. A compound in which Z═OH, R2═NH2, R1═R4═H, R3═SO3H;
    • 20. A compound wherein Z═OH, R2═NH2, R═R4═H, R3═OSO3H;
    • 21. A compound wherein Z═OH, R2═NH2, R1═R3═H, R4═SO3H;
    • 22. A compound wherein Z═OH, R2═NH2, R1═R3═H, R4═OSO3H;
    • 23. A compound wherein R1═NHSO3H, R2═R3═R4═H;
    • 24. A compound wherein Z═NH2, R1═H, R2═CH2SO3H;
    • 25. A compound wherein Z═NH2, R1═H, R2═CH2OSO3H;
    • 26. A compound wherein Z═NH2, R1═H, R2═SO3H;
    • 27. A compound wherein Z═NH2, R1═H, R2═OSO3H;
    • 28. A compound wherein Z═R1═NH2, R2═R4═H, R2═SO3H;
    • 29. A compound wherein Z═R1═NH2, R2═R4═H, R3═OSO3H;
    • 30. A compound wherein Z═R1═NH2, R2═R4═H, R3═SO3H;
    • 31. A compound wherein Z═R1═NH2, R2═R4═H, R3═OSO3H;
    • 32. A compound wherein Z═R1═NH2, R2═R3═H, R4═SO3H;
    • 33. A compound wherein Z═R1═NH2, R2═R3═H, R4═OSO3H;
    • 34. A compound wherein Z═NH2, R2═NHSO3H, R1═R3═R4═H;
    • 35. A compound wherein Z═NH2, R2 to R4═H, R1═CH2SO3H;
    • 36. A compound wherein Z═NH2, R2 to R4═H, R1═CH2SO3H;
    • 37. A compound wherein Z═OH, R2 to R4═H, R1═SO3H;
    • 38. A compound wherein Z═OH, R2 to R4═H, R1═OSO3H;
    • 39. A compound wherein Z═R2═NH2, R3═R4═H, R1═SO3H;
    • 40. A compound wherein Z═R2═NH2, R3═R4═H, R1═OSO3H;
    • 41. A compound wherein Z═R2═NH2, R1═R4═H, R3═SO3H;
    • 42. A compound wherein Z═R2═NH2, R1═R4═H, R3═OSO3H;
    • 43. A compound wherein Z═R2═NH2, R1═R3═H, R4═SO3H;
    • 44. A compound wherein Z═R2═NH2, R1═R3═H, R4═OSO3H;


      f) a process for the preparation of sulfonic acid/sulfate derivatives of the formula (Ib) and non-toxic salts thereof:
    • 1. A compound wherein Z═OH, R1═NHSO3H, R2═R3═R4═R5═R6═H;
    • 2. A compound wherein Z═OH, R1, R3 to R6═H, R2═CH2SO3H;
    • 3. A compound wherein Z═OH, R1, R3 to R6═H, R2═CH2OSO3H;
    • 4. A compound wherein Z═OH, R1, R3 to R6═H, R2═SO3H;
    • 5. A compound wherein Z═OH, R1, R3 to R6═H, R2═OSO3H;
  • 6. A compound wherein Z═OH, R2 to R6═H, R1═OSO3H;
  • 7. A compound wherein Z═OH, R2 to R6═H, R1═SO3H;
  • 8. A compound wherein Z═OH, R1═NH2, R3 to R6═H, R2═SO3H;
  • 9. A compound wherein Z═OH, R1═NH2, R3 to R6═H, R2═OSO3H;
    • 10. A compound wherein Z═OH, R1═NH2, R2═H, R4 to R6═H, R3═SO3H;
    • 11. A compound wherein Z═OH, R1═NH2, R2═H, R4 to R6═H, R3═OSO3H;
    • 12. A compound wherein Z═OH, R1═NH2, R2═R3═R5═R6═H, R4═SO3H;
    • 13. A compound wherein Z═OH, R1═NH2, R2═R3═R5═R6═H, R4═OSO3H;
    • 14. A compound wherein Z═OH, R1═NH2, R2═R3═R4═R6═H, R5═SO3H;
    • 15. A compound wherein Z═OH, R1═NH2, R2═R3═R4═R6═H, R5═OSO3H;
    • 16. A compound wherein Z═OH, R1═NH2, R2 to R5═H, R6═SO3H;
    • 17. A compound wherein Z═OH, R1═NH2, R2 to R5═H, R6═OSO3H;
    • 18. A compound wherein Z═OH, R2═NHSO3H, R1, R3 to R6═H;
    • 19. A compound wherein Z═OH, R2 to R6═H, R1═CH2SO3H;
    • 20. A compound wherein Z═OH, R2 to R6═H, R1═CH2OSO3H;
  • 21. A compound wherein Z═OH, R2═NH2, R3 to R6H, R1═SO3H;
    • 22. A compound wherein Z═OH, R2═NH2, R3 to R6H, R1═OSO3H;
    • 23. A compound wherein Z═OH, R2═NH2, R1, R4 to R6H, R3═SO3H;
    • 24. A compound wherein Z═OH, R2═NH2, R1, R4 to R6H, R3═OSO3H;
    • 25. A compound wherein Z═OH, R2═NH2, R1═R3═R5═R6═H, R4═SO3H;
    • 26. A compound wherein Z═OH, R2═NH2, R1═R3═R5═R6═H, R4═OSO3H;
    • 27. A compound wherein Z═OH, R2═NH2, R1═R3═R4═R6═H, R5═SO3H;
    • 28. A compound wherein Z═OH, R2═NH2, R1═R3═R4═R6═H, R5═OSO3H;
    • 29. A compound wherein Z═OH, R2═NH2, R1═R3═R4═R5═H, &═SO3H;
    • 30. A compound wherein Z═OH, R2═NH2, R1═R3═R4═R5═H, R6═OSO3H;
    • 31. A compound wherein Z═NH2, R1═NHSO3H, R2 to R6═H;
    • 32. A compound wherein Z═R1═NH2, R3 to R6═H, R2═SO3H;
    • 33. A compound wherein Z═R1═NH2, R3 to R6═H, R2═OSO3H;
    • 34. A compound wherein Z═R1═NH2, R2═H, R3 to R6═H, R3═SO3H;
    • 35. A compound wherein Z═R1═NH2, R2═H, R3 to R6═H, R3═OSO3H;
    • 36. A compound wherein Z═R1═NH2, R2═R3═R5═R6═H, R4═SO3H;
    • 37. A compound wherein Z═R1═NH2, R2═R3═R5═R6═H, R4═OSO3H;
    • 38. A compound wherein Z═R1═NH2, R2═R3═R4═R6═H, R5═SO3H;
    • 39. A compound wherein Z═R1═NH2, R2═R3═R4═R6═H, R5═OSO3H;
    • 40. A compound wherein Z═R1═NH2, R2 to R5═H, R6═SO3H;
    • 41. A compound wherein Z═R1═NH2, R2 to R5═H, R6═OSO3H;
    • 42. A compound wherein Z═NH2, R1, R3 to R6═H, R2═CH2SO3H;
    • 43. A compound wherein Z═NH2, R1, R3 to R6═H, R2═CH2OSO3H;
    • 44. A compound wherein Z═NH2, R1, R3 to R6═H, R2═SO3H;
    • 45. A compound wherein Z═NH2, R1, R3 to R6═H, R2═OSO3H;
    • 46. A compound wherein Z═NH2, R2 to R6═H, R1═OSO3H;
    • 47. A compound wherein Z═NH2, R2 to R6═H, R1═SO3H;
    • 48. A compound wherein Z═NH2, R2═NHSO3H; R1═H, R3 to R6═H;
    • 49. A compound wherein Z═NH2, R2 to R6═H, R1═CH2SO3H;
    • 50. A compound wherein Z═NH2, R2 to R6═H, R1═CH2OSO3H;
    • 51. A compound wherein Z═NH2, R2═NH2, R3 to R6═H, R1═SO3H;
    • 52. A compound wherein Z═NH2, R2═NH2, R3 to R6═H, R1═OSO3H;
    • 53. A compound wherein Z═NH2, R2═NH2, R1, R4 to R6H, R3═SO3H;
    • 54. A compound wherein Z═R2═NH2, R1, R4 to R6H, R3═OSO3H;
    • 55. A compound wherein Z═R2═NH2, R1═R3═R5═R6═H, R4═SO3H;
    • 56. A compound wherein Z═R2═NH2, R1═R3═R5═R6═H, R4═OSO3H;
    • 57. A compound wherein Z═R2═NH2, R1═R3═P4═R6═H, R5═SO3H;
    • 58. A compound wherein Z═R2═NH2, R1═R3═R4═R6═H, R5═OSO3H;
    • 59. A compound wherein Z═R2═NH2, R1═R3═R4═R5═H, R6═SO3H;
    • 60. A compound wherein Z═R2═NH2, R1═R3═R4═R5═H, R6═OSO3H;


      g) a process for the preparation of sulfonic acid/sulfate derivatives of the formula (Ic) and non-toxic salts thereof:
    • 1. A compound wherein Z═OH, R1═NHSO3H, R2 to R8═H;
    • 2. A compound wherein Z═OH, R1, R3 to R8═H, R2═CH2SO3H;
    • 3. A compound wherein Z═OH, R1, R3 to R8═H, R2═CH2OSO3H;
    • 4. A compound wherein Z═OH, R1, R3 to R8═H, R2═SO3H;
    • 5. A compound wherein Z═OH, R1, R3 to R8═H, R2═OSO3H;
    • 6. A compound wherein Z═OH, R1═NH2, R3 to R8═H, R2═SO3H;
    • 7. A compound wherein Z═OH, R1═NH2, R3 to R8═H, R2═OSO31H;
    • 8. A compound wherein Z═OH, R1═NH2, R2═H, R4 to R8═H, R3═SO3H;
    • 9. A compound wherein Z═OH, R1═NH2, R2═H, R4 to R8═H, R3═OSO3H;
    • 10. A compound wherein Z═OH, R1═NH2, R2═R3═H, R5 to R8═H, R4═SO3H;
    • 11. A compound wherein Z═OH, R1═NH2, R2═R3═H, R5 to R8═H, R4═OSO3H;
    • 12. A compound wherein Z═OH, R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═SO3H;
    • 13. A compound wherein Z═OH, R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═OSO3H;
    • 14. A compound wherein Z═OH, R1═NH2, R2═R5═H, R7═R8═H, R6═SO3H;
    • 15. A compound wherein Z═OH, R1═NH2, R2═R5═H, R7═R8═H, R6═OSO3H;
    • 16. A compound wherein Z═OH, R1═NH2, R2 to R6═H, R8═H, R7═SO3H
    • 17. A compound wherein Z═OH, R1═NH2, R2 to R6═H, R8═H, R7═OSO3H;
    • 18. A compound wherein Z═OH, R1═NH2, R2 to R7═H, R8═SO3H;
    • 19. A compound wherein Z═OH, R1═NH2, R2 to R7═H, R8═OSO3H;
    • 20. A compound wherein Z═OH, R2═NHSO3H, R1, R3 to R8═H;
    • 21. A compound wherein Z═OH, R2 to R8═H, R1═CH2SO3H;
    • 22. A compound wherein Z═OH, R2 to R8═H, R1═CH2OSO3H;
    • 23. A compound wherein Z═OH, R2 to R8═H, R1═SO3H;
    • 24. A compound wherein Z═OH, R2 to R8═H, R1═OSO3H;
    • 25. A compound wherein Z═OH, R2═NH2, R3 to R8═H, R1═SO3H;
    • 26. A compound wherein Z═OH, R2═NH2, R3 to R8═H, R1═OSO3H;
    • 27. A compound wherein Z═OH, R2═NH2, R1, R4 to R8═H, R3═SO3H;
    • 28. A compound wherein Z═OH, R2═NH2, R1, R4 to R8═H, R3═OSO3H;
    • 29. A compound wherein Z═OH, R2═NH2, R1═R3═H, R5 to R8═H, R4═SO3H;
    • 30. A compound wherein Z═OH, R2═NH2, R1═R3═H, R5 to R8═H, R4═OSO3H;
    • 31. A compound wherein Z═OH, R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═SO3H;
    • 32. A compound wherein Z═OH, R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═OSO3H;
    • 33. A compound wherein Z═OH, R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═SO3H;
    • 34. A compound wherein Z═OH, R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═OSO3H;
    • 35. A compound wherein Z═OH, R2═NH2, R═R8═H, R3 to R6═H, R7═SO3H;
    • 36. A compound wherein Z═OH, R2═NH2, R1═R8═H, R3 to R6═H, R7═OSO3H;
    • 37. A compound wherein Z═OH, R2═NH2, R1═H, R3 to R7═H, R8═SO3H;
    • 38. A compound wherein Z═OH, R2═NH2, R1═H, R3 to R7═H, R8═OSO3H;
    • 39. A compound wherein Z═NH2, R1═NHSO3H, R2 to R8═H;
    • 40. A compound wherein Z═NH2, R1 and R3 to R8═H, R2═CH2SO3H;
    • 41. A compound wherein Z═NH2, R1 and R3 to R8═H, R2═CH2OSO3H;
    • 42. A compound wherein Z═NH2, R1 and R3 to R8═H, R2═SO3H;
    • 43. A compound wherein Z═NH2, R1 and R3 to R8═H, R2═OSO3H;
    • 44. A compound wherein Z═R1═NH2, R3 to R8═H, R2═SO3H;
    • 45. A compound wherein Z═R1═NH2, R3 to R8═H, R2═OSO3H;
    • 46. A compound wherein Z═R1═NH2, R2═H, R4 to R8═H, R3═SO3H;
    • 47. A compound wherein Z═R1═NH2, R2═H, R4 to R8═H, R3═OSO3H;
    • 48. A compound wherein Z═R1═NH2, R2═R3═H, R5 to R8═H, R4═SO3H;
    • 49. A compound wherein Z═R1═NH2, R2═R3═H, R5 to R8═H, R4═OSO3H;
    • 50. A compound wherein Z═R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═SO3H;
    • 51. A compound wherein Z═R1═NH2, R2═R3═R4═H, R6 to R8═H, R5═OSO3H;
    • 52. A compound wherein Z═R1═NH2, R2═R5═H, R7═R8═H, R6═SO3H;
    • 53. A compound wherein Z═R1═NH2, R2═R5═H, R7═R8═H, R6═OSO3H;
    • 54. A compound wherein Z═R1═NH2, R2 to R6═H, R8═H, R7═SO3H;
    • 55. A compound wherein Z═R1═NH2, R2 to R6═H, R8═H, R7═OSO3H;
    • 56. A compound wherein Z═R1═NH2, R2 to R7═H, R8═SO3H;
    • 57. A compound wherein Z═R1═NH2, R2 to R7═H, R8═OSO3H;
    • 58. A compound wherein Z═NH2, R2═NHSO3H, R1 and R3 to R8═H;
    • 59. A compound wherein Z═NH2, R2 to R8═H, R1═CH2SO3H;
    • 60. A compound wherein Z═NH2, R2 to R8═H, R1═CH2OSO3H;
    • 61. A compound wherein Z═NH2, R2 to R8═H, R1═SO3H;
    • 62. A compound wherein Z═NH2, R2 to R8═H, R1═OSO3H;
    • 63. A compound wherein Z═R2═NH2, R3 to R8═H, R1═SO3H;
    • 64. A compound wherein Z═R2═NH2, R3 to R8═H, R1═OSO3H;
    • 65. A compound wherein Z═R2═NH2, R1, R4 to R8═H, R3═SO3H;
    • 66. A compound wherein Z═R2═NH2, R1, R4 to R8═H, R3═OSO3H;
    • 67. A compound wherein Z═R2═NH2, R1═R3═H, R5 to R8═H, R4═SO3H;
    • 68. A compound wherein Z═R2═NH2, R1═R3═H, R5 to R8═H, R4═OSO3H;
    • 69. A compound wherein Z═R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═SO
    • 70. A compound wherein Z═R2═NH2, R1═R3═R4═H, R6 to R8═H, R5═OSO3H;
    • 71. A compound wherein Z═R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═SO3H;
    • 72. A compound wherein Z═R2═NH2, R1═H, R3 to R5═H, R7═R8═H, R6═OSO3H;
    • 73. A compound wherein Z═R2═NH2, R1═R8═H, R3 to R6═H, R7═SO3H;
    • 74. A compound wherein Z═R2═NH2, R1═R8═H, R3 to R6═H, R7═OSO3H;
    • 75. A compound wherein Z═R2═NH2, R1═H, R3 to R7═H, R8═SO3H;
    • 76. A compound wherein Z═R2═NH2, R1═H, R3 to R7═H, R8═OSO3H.


      h) In the compound of the present invention of the formula (Ia) wherein the compound is selected from the group consisting of aspartic acid, asparagine and corresponding de-amino analogs:
    • 1. L-Aspartic acid, N-Sulfonic acid
    • 2. L-Aspartic acid, 2β-sulfonic acid
    • 3. L-Aspartic acid, 2β-sulfate
    • 4. L-aspartic acid, 3α-sulfonic acid
    • 5. L-aspartic acid, 3α-sulfate
    • 6. L-aspartic acid, 3β-sulfonic acid
    • 7. L-aspartic acid, 3β-sulfate
    • 8. 2α,3-dicarboxy, propane-1-sulfonic acid
    • 9. 2α,3-dicarboxy, propane-1-sulfate
    • 10. 1α,2-carboxy ethane sulfonic acid
    • 11. 1α,2-carboxy ethane sulfate
    • 12. D-aspartic acid, N-sulfonic acid
    • 13. 2β,3-carboxy, propane-1-sulfonic acid
    • 14. 2β,3-carboxy, propane-1-sulfate
    • 15. 1β,2-carboxy ethane-1-sulfonic acid
    • 16. 1β,2-carboxy ethane-1-sulfate
    • 17. D-aspartic acid, 2α-sulfonic acid
    • 18. D-aspartic acid, 2α-sulfonic acid
    • 19. D-Aspartic acid, 3α-sulfonic acid
    • 20. D-Aspartic acid, 3α-sulfate
    • 21. D-Aspartic acid, 3β-sulfonic acid
    • 22. D-aspartic acid, 3β-sulfate
    • 23. L-asparagine, N-sulfonic acid
    • 24. 2α-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 25. 2α-carboxy, 3-carboxamido, propane-1-sulfate
    • 26. 1α-carboxy, 2-carboxamido, ethane sulfonic acid
    • 27. 1α-carboxy, 2-carboxamido, ethane sulfate
    • 28. L-asparagine, 2β-sulfonic acid
    • 29. -asparagine, 2β-sulfate
    • 30. L-asparagine, 3α-sulfonic acid
    • 31. L-asparagine, 3α-sulfate
    • 32. L-asparagine, 3β-sulfonic acid
    • 33. L-asparagine, 3β-sulfate
    • 34. D-asparagine, N-sulfonic acid
    • 35. 2β-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 36. 2β-carboxy, 3-carboxamido, propane-1-sulfate
    • 37. 1β-carboxy, 2-carboxamido, ethane sulfonic acid
    • 38. 1β-carboxy, 2-carboxamido, ethane sulfate
    • 39. D-asparagine, 2α-sulfonic acid
    • 40. D-asparagine, 2α-sulfate
    • 41. D-asparagine, 3α-sulfonic acid
    • 42. D-asparagine, 3α-sulfate
    • 43. D-asparagine, 3β-sulfonic acid
    • 44. D-asparagine, 3β-sulfate.


      i) In the compound of the present invention of the formula (Ib) wherein the compound is selected from the group consisting of glutamic acid, glutamine and corresponding de-amino analogs:
    • 1. 1 L-glutamic acid, N-sulfonic acid
    • 2. 2α,4-dicarboxy, butane-1-sulfonic acid
    • 3. 2α,4-dicarboxy, butane-1-sulfate
    • 4. 1α,3-dicarboxy, propane sulfonic acid
    • 5. 1α,3-dicarboxy, propane sulfate
    • 6. 1β,3-dicarboxy, propane sulfate
    • 7. 1β,3-dicarboxy, propane sulfonic acid
    • 8. L-glutamic acid, 2β-sulfonic acid
    • 9. L-glutamic acid, 2β-sulfate
    • 10. L-glutamic acid, 3α-sulfonic acid
    • 11. L-glutamic acid, 3α-sulfate
    • 12. L-glutamic acid, 3β-sulfonic acid
    • 13. L-glutamic acid, 3β-sulfate
    • 14. L-glutamic acid, 4α-sulfonic acid
    • 15. L-glutamic acid, 4α-sulfate
    • 16. L-glutamic acid, 4β-sulfonic acid
    • 17. L-glutamic acid, 4β-sulfate
    • 18. D-glutamic acid, N-sulfonic acid
    • 19. 2β,4-dicarboxy, butane-1-sulfonic acid
    • 20. 2β,4-dicarboxy, butane-1-sulfate
    • 21. D-glutamic acid, 2α-sulfonic acid
    • 22. D-glutamic acid, 2α-sulfate
    • 23. D-glutamic acid, 3α-sulfonic acid
    • 24. D-glutamic acid, 3α-sulfate
    • 25. D-glutamic acid, 3β-sulfonic acid
    • 26. D-glutamic acid, 3β-sulfate
    • 27. D-glutamic acid, 4α-sulfonic acid
    • 28. D-glutamic acid, 4α-sulfate
    • 29. D-glutamic acid, 4β-sulfonic acid
    • 30. D-glutamic acid, 4β-sulfate
    • 31. L-glutamine, N-sulfonic acid
    • 32. L-glutamine, 2β-sulfonic acid
    • 33. L-glutamine, 2β-sulfate
    • 34. L-glutamine, 3α-sulfonic acid
    • 35. L-glutamine, 3α-sulfate
    • 36. L-glutamine, 3β-sulfonic acid
    • 37. L-glutamine, 3β-sulfate
    • 38. L-glutamine, 4α-sulfonic acid
    • 39. L-glutamine, 4α-sulfate
    • 40. L-glutamine, 4β-sulfonic acid
    • 41. L-glutamine, 4β-sulfate
    • 42. 2α-carboxy, 4-carboxamido, butane-1-sulfonic acid
    • 43. 2α-carboxy, 4-carboxamido, butane-1-sulfate
    • 44. 1α-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 45. 1α-carboxy, 3-carboxamido, propane-1-sulfate
    • 46. 1β-carboxy, 3-carboxamido, propane-1-sulfate
    • 47. 1β-carboxy, 3-carboxamido, propane-1-sulfonic acid
    • 48. D-glutamine, N-sulfonic acid
    • 49. 2β-carboxy, 4-carboxamido, butane-1-sulfonic acid
    • 50. 2β-carboxy, 4-carboxamido, butane-1-sulfate
    • 51. D-glutamine, 2α-sulfonic acid
    • 52. D-glutamine, 2α-sulfate
    • 53. D-glutamine, 3α-sulfonic acid
    • 54. D-glutamine, 3α-sulfate
    • 55. D-glutamine, 3β-sulfonic acid
    • 56. D-glutamine, 3β-sulfate
    • 57. D-glutamine, 4α-sulfonic acid
    • 58. D-glutamine, 4α-sulfate
    • 59. D-glutamine, 4β-sulfonic acid
    • 60. D-glutamine, 4β-sulfate


      j) In the compound of the present invention of the formula (Ic) wherein the compound is selected from the group consisting of homoglutamic acid, homoglutamine and corresponding de-amino analogs:
    • 1. L-homoglutamic acid, N-sulfonic acid
    • 2. Pentane-2α,5-dicarboxy-1-sulfonic acid
    • 3. Pentane-2α,5-dicarboxy-1-sulfate
    • 4. Butane-1α,4-dicarboxy-1-sulfonic acid
    • 5. Butane-1α,4-dicarboxy-1-sulfate
    • 6. L-homoglutamic acid, 2β-sulfonic acid
    • 7. L-homoglutamic acid, 2β-sulfate
    • 8. L-homoglutamic acid, 3α-sulfonic acid
    • 9. L-homoglutamic acid, 3α-sulfate
    • 10. L-homoglutamic acid, 3β-sulfonic acid
    • 11. L-homoglutamic acid, 3β-sulfate
    • 12. L-homoglutamic acid, 4α-sulfonic acid
    • 13. L-homoglutamic acid, 4α-sulfate
    • 14. L-homoglutamic acid, 4β-sulfonic acid
    • 15. L-homoglutamic acid, 4β-sulfate
    • 16. L-homoglutamic acid, 5α-sulfonic acid
    • 17. L-homoglutamic acid, 5α-sulfate
    • 18. L-homoglutamic acid, 5β-sulfonic acid
    • 19. L-homoglutamic acid, 5β-sulfate
    • 20. D-homoglutamic acid, N-sulfonic acid
    • 21. Pentane-2β,5-dicarboxy-1-sulfonic acid
    • 22. Pentane-2β,5-dicarboxy-1-sulfate
    • 23. Butane-1β,4-dicarboxy-1-sulfonic acid
    • 24. Butane-1β,4-dicarboxy-1-sulfate
    • 25. D-homoglutamic acid, 2α-sulfonic acid
    • 26. D-homoglutamic acid, 2α-sulfate
    • 27. D-homoglutamic acid, 3α-sulfonic acid
    • 28. D-homoglutamic acid, 3α-sulfate
    • 29. D-homoglutamic acid, 3β-sulfonic acid
    • 30. D-homoglutamic acid, 3β-sulfate
    • 31. D-homoglutamic acid, 4α-sulfonic acid
    • 32. D-homoglutamic acid, 4α-sulfate
    • 33. D-homoglutamic acid, 4β-sulfonic acid
    • 34. D-homoglutamic acid, 4β-sulfate
    • 35. D-homoglutamic acid, 5α-sulfonic acid
    • 36. D-homoglutamic acid, 5α-sulfate
    • 37. D-homoglutamic acid, 5β-sulfonic acid
    • 38. D-homoglutamic acid, 5β-sulfate
    • 39. L-homoglutamine, N-sulfonic acid
    • 40. Pentane-2α-carboxy, 5-carboxamido-1-sulfonic acid
    • 41. Pentane-2α-carboxy, 5-carboxamido-1-sulfate
    • 42. Butane-1α-carboxy, 4-carboxamido-1-sulfonic acid
    • 43. Butane-1α-carboxy, 4-carboxamido-1-sulfate
    • 44. L-homoglutamine, 2β-sulfonic acid
    • 45. L-homoglutamine, 2β-sulfate
    • 46. L-homoglutamine, 3α-sulfonic acid
    • 47. L-homoglutamine, 3α-sulfate
    • 48. L-homoglutamine, 3β-sulfonic acid
    • 49. L-homoglutamine, 3β-sulfate
    • 50. L-homoglutamine, 4α-sulfonic acid
    • 51. L-homoglutamine, 4α-sulfate
    • 52. L-homoglutamine, 4β-sulfonic acid
    • 53. L-homoglutamine, 4β-sulfate
    • 54. L-homoglutamine, 5α-sulfonic acid
    • 55. L-homoglutamine, 5α-sulfate
    • 56. L-homoglutamine, 5β-sulfonic acid
    • 57. L-homoglutamine, 5β-sulfate
    • 58. D-homoglutamine, N-sulfonic acid
    • 59. Pentane-2β-carboxy, 5-carboxamido-1-sulfonic acid
    • 60. Pentane-2β-carboxy, 5-carboxamido-1-sulfate
    • 61. Butane-1β-carboxy, 4-carboxamido-1-sulfonic acid
    • 62. Butane-1β-carboxy, 4-carboxamido-1-sulfate
    • 63. D-homoglutamine, 2α-sulfonic acid
    • 64. D-homoglutamine, 2α-sulfate
    • 65. D-homoglutamine, 3α-sulfonic acid
    • 66. D-homoglutamine, 3α-sulfate
    • 67. D-homoglutamine, 3β-sulfonic acid
    • 68. D-homoglutamine, 3β-sulfate
    • 69. D-homoglutamine, 4α-sulfonic acid
    • 70. D-homoglutamine, 4α-sulfate
    • 71. D-homoglutamine, 4β-sulfonic acid
    • 72. D-homoglutamine, 4β-sulfate
    • 73. D-homoglutamine, 5α-sulfonic acid
    • 74. D-homoglutamine, 5α-sulfate
    • 75. D-homoglutamine, 5β-sulfonic acid
    • 76. D-homoglutamine, 5β-sulfate


The preferable specific compounds of the formulae (Ia), (Ib) and (Ic) are the derivatives of aspartic acid, asparagine and corresponding de-amino analogs (Table 1), glutamic acid, glutamine and corresponding de-amino analogs (Table 2) and homoglutamic acid, homoglutamine and corresponding de-amino analogs (Table 3) and non toxic salts thereof and example compounds.

TABLE 1embedded image1.L-Aspartic acid, N-Sulfonic acidZ = OH, R1 = NHSO3H, R2 = R3 = R4 = H2.L-Aspartic acid, 2β-sulfonic acidZ = OH, R1 = NH2, R3 = R4 = H, R2 = SO3H3.L-Aspartic acid, 2β-sulfateZ = OH, R1 = NH2, R3 = R4 = H, R2 = OSO3H4.L-aspartic acid, 3α-sulfonic acidZ = OH, R1 = NH2, R2 = R4 = H, R3 = SO3H5.L-aspartic acid, 3α-sulfateZ = OH, R1 = NH2, R2 = R4 = H, R3 = OSO3H6.L-aspartic acid, 3β-sulfonic acidZ = OH, R1 = NH2, R2 = R3 = H, R4 = SO3H7.L-aspartic acid, 3β-sulfateZ = OH, R1 = NH2, R2 = R3 = H, R4 = OSO3H8.2α, 3-dicarboxy, propane-1-sulfonic acidZ = OH, R1 = R3 = R4 = H, R2 = CH2SO3H9.2α, 3-dicarboxy, propane-1-sulfateZ = OH, R1 = R3 = R4 = H, R2 = CH2OSO3H10.1α, 2-carboxy ethane sulfonic acidZ = OH, R1 = R3 = R4 = H, R2 = SO3H11.1α, 2-carboxy ethane sulfateZ = OH, R1 = R3 = R4 = H, R2 = OSO3H12.D-aspartic acid, N-sulfonic acidZ = OH, R2 = NHSO3H, R1 = R3= R4 = H13.2β, 3-carboxy, propane-1-sulfonic acidZ = OH, R2 = H, R1 = CH2SO3H14.2β, 3-carboxy, propane-1-sulfateZ = OH, R2 = H, R1 = CH2OSO3H15.1β, 2-carboxy ethane-1-sulfonic acidZ = OH, R2 = H, R1 = SO3H16.1β, 2-carboxy ethane-1-sulfateZ = OH, R2 = H, R1 = OSO3H17.D-aspartic acid, 2α-sulfonic acidZ = OH, R2 = NH2, R3 = R4 = H, R1 = SO3H18.D-aspartic acid, 2α-sulfonic acidZ = OH, R2 = NH2, R3 = R4 = H, R1 = SO3H19.D-Aspartic acid, 3α-sulfonic acidZ = OH, R2 = NH2, R1 = R4 = H, R3 = SO3H20.D-Aspartic acid, 3α-sulfateZ = OH, R2 = NH2, R1 = R4 = H, R3 = OSO3H21.D-Aspartic acid, 3β-sulfonic acidZ = OH, R2 = NH2, R1 = R3 = H, R4 = SO3H22.D-aspartic acid, 3β-sulfateZ = OH, R2 = NH2, R1 = R3 = H, R4 = OSO3H23.L-asparagine, N-sulfonic acidZ = NH2, R1 = NHSO3H, R2 = R3 = R4 = H24.2α-carboxy, 3-carboxamido, propane-1-sulfonic acidZ = NH2, R1 = H, R2 = CH2SO3H25.2α-carboxy, 3-carboxamido, propane-1-sulfateZ = NH2, R1 = H, R2 = CH2OSO3H26.1α-carboxy, 2-carboxamido, ethane sulfonic acidZ = NH2, R1 = H, R2 = SO3H27.1α-carboxy, 2-carboxamido, ethane sulfateZ = NH2, R1 = H, R2 = OSO3H28.L-asparagine, 2β-sulfonic acidZ = R1 = NH2, R2 = R4 = H, R2 = SO3H29.L-asparagine, 2β-sulfateZ = R1 = NH2, R2 = R4 = H, R3 = OSO3H30.L-asparagine, 3α-sulfonic acidZ = R1 = NH2, R2 = R4 = H, R3 = SO3H31.L-asparagine, 3α-sulfateZ = R1 = NH2, R2 = R4 = H, R3 = OSO3H32.L-asparagine, 3β-sulfonic acidZ = R1 = NH2, R2 = R3 = H, R4 = SO3H33.L-asparagine, 3β-sulfateZ = R1 = NH2, R2 = R3 = H, R4 = OSO3H34.D-asparagine, N-sulfonic acidZ = NH2, R2 = NHSO3H, R1 = R3 = R4 = H35.2β-carboxy, 3-carboxamido, propane-1-sulfonic acidZ = NH2, R2 to R4 = H, R1 = CH2SO3H36.2β-carboxy, 3-carboxamido, propane-1-sulfateZ = NH2, R2 to R4 = H, R1 = CH2SO3H37.1β-carboxy, 2-carboxamido, ethane sulfonic acid]Z = OH, R2 to R4 = H, R1 = SO3H38.1β-carboxy, 2-carboxamido, ethane sulfateZ = OH, R2 to R4 = H, R1 = OSO3H39.D-asparagine, 2α-sulfonic acidZ = R2 = NH2, R3 = R4 = H, R1 = SO3H40.D-asparagine, 2α-sulfateZ = R2 = NH2, R3 = R4 = H, R1 = OSO3H41.D-asparagine, 3α-sulfonic acidZ = R2 = NH2, R1 = R4 = H, R3 = SO3H42.D-asparagine, 3α-sulfateZ = R2 = NH2, R1 = R4 = H, R3 = OSO3H43.D-asparagine, 3β-sulfonic acidZ = R2 = NH2, R1 = R3 = H, R4 = SO3H44.D-asparagine, 3β-sulfateZ = R2 = NH2, R1 = R3 = H, R4 = OSO3H









TABLE 2













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1.
L-glutamic acid, N-sulfonic acid
Z = OH, R1 = NHSO3H, R2 = R3 = R4 = R5 = R6 = H


2.
2α, 4-dicarboxy, butane-1-sulfonic acid
Z = OH, R1, R3 to R6 = H, R2 = CH2SO3H


3.
2α, 4-dicarboxy, butane-1-sulfate
Z = OH, R1, R3 to R6 = H, R2 = CH2OSO3H


4.
1α, 3-dicarboxy, propane sulfonic acid
Z = OH, R1, R3 to R6 = H, R2 = SO3H


5.
1α, 3-dicarboxy, propane sulfate
Z = OH, R1, R3 to R6 = H, R2 = OSO3H


6.
1β, 3-dicarboxy, propane sulfate
Z = OH, R2 to R6 = H, R1 = OSO3H


7.
1β, 3-dicarboxy, propane sulfonic acid
Z = OH, R2 to R6 = H, R1 = SO3H


8.
L-glutamic acid, 2β-sulfonic acid
Z = OH, R1 = NH2, R3 to R6 = H, R2 = SO3H


9.
L-glutamic acid, 2β-sulfate
Z = OH, R1 = NH2, R3 to R6 = H, R2 = OSO3H


10.
L-glutamic acid, 3α-sulfonic acid
Z = OH, R1 = NH2, R2 = H, R4 to R6 = H, R3 = SO3H


11.
L-glutamic acid, 3α-sulfate
Z = OH, R1 = NH2, R2 = H, R4 to R6 = H, R3 = OSO3H


12.
L-glutamic acid, 3β-sulfonic acid
Z = OH, R1 = NH2, R2 = R3 = R5 = R6 = H, R4 = SO3H


13.
L-glutamic acid, 3β-sulfate
Z = OH, R1 = NH2, R2 = R3 = R5 = R6 = H, R4 = OSO3H


14.
L-glutamic acid, 4α-sulfonic acid
Z = OH, R1 = NH2, R2 = R3 = R4 = R6 = H, R5 = SO3H


15.
L-glutamic acid, 4α-sulfate
Z = OH, R1 = NH2, R2 = R3 = R4 = R6 = H, R5 = OSO3H


16.
L-glutamic acid, 4β-sulfonic acid
Z = OH, R1 = NH2, R2 to R5 = H, R6 = SO3H


17.
L-glutamic acid, 4β-sulfate
Z = OH, R1 = NH2, R2 to R5 = H, R6 = OSO3H


18.
D-glutamic acid, N-sulfonic acid
Z = OH, R2 = NHSO3H, R1, R3 to R6 = H


19.
2β, 4-dicarboxy, butane-1-sulfonic acid
Z = OH, R2 to R6 = H, R1 = CH2SO3H


20.
2β, 4-dicarboxy, butane-1-sulfate
Z = OH, R2 to R6 = H, R1 = CH2OSO3H


21.
D-glutamic acid, 2α-sulfonic acid
Z = OH, R2 = NH2, R3 to R6 H, R1 = SO3H


22.
D-glutamic acid, 2α-sulfate
Z = OH, R2 = NH2, R3 to R6 H, R1 = OSO3H


23.
D-glutamic acid, 3α-sulfonic acid
Z = OH, R2 = NH2, R1, R4 to R6 H, R3 = SO3H


24.
D-glutamic acid, 3α-sulfate
Z = OH, R2 = NH2, R1, R4 to R6 H, R3 = OSO3H


25.
D-glutamic acid, 3β-sulfonic acid
Z = OH, R2 = NH2, R1 = R3 = R5 = R6 = H, R4 = SO3H


26.
D-glutamic acid, 3β-sulfate
Z = OH, R2 = NH2, R1 = R3 = R5 = R6 = H, R4 = OSO3H


27.
D-glutamic acid, 4α-sulfonic acid
Z = OH, R2 = NH2, R1 = R3 = R4 = R6 = H, R5 = SO3H


28.
D-glutamic acid, 4α-sulfate
Z = OH, R2 = NH2, R1 = R3 = R4 = R6 = H, R5 = OSO3H


29.
D-glutamic acid, 4β-sulfonic acid
Z = OH, R2 = NH2, R1 = R3 = R4 = R5 = H, R6 = SO3H


30.
D-glutamic acid, 4β-sulfate
Z = OH, R2 = NH2, R1 = R3 = R4 = R5 = H, R6 = OSO3H


31.
L-glutamine, N-sulfonic acid
Z = NH2, R1 = NHSO3H, R2 to R6 = H


32.
L-glutamine, 2β-sulfonic acid
Z = R1 = NH2, R3 to R6 = H, R2 = SO3H


33.
L-glutamine, 2β-sulfate
Z = R1 = NH2, R3 to R6= H, R2 = OSO3H


34.
L-glutamine, 3α-sulfonic acid
Z = R1 = NH2, R2 = H, R3 to R6 = H, R3 = SO3H


35.
L-glutamine, 3α-sulfate
Z = R1 = NH2, R2 = H, R3 to R6 = H, R3 = OSO3H


36.
L-glutamine, 3β-sulfonic acid
Z = R1 = NH2, R2 = R3 = R5 = R6 = H, R4 = SO3H


37.
L-glutamine, 3β-sulfate
Z = R1 = NH2, R2 = R3 = R5 = R6 = H, R4 = OSO3H


38.
L-glutamine, 4α-sulfonic acid
Z = R1 = NH2, R2 = R3 = R4 = R6 = H, R5 = SO3H


39.
L-glutamine, 4α-sulfate
Z = R1 = NH2, R2 = R3 = R4 = R6 = H, R5 = OSO3H


40.
L-glutamine, 4β-sulfonic acid
Z = R1 = NH2, R2 to R5 = H, R6 = SO3H


41.
L-glutamine, 4β-sulfate
Z = R1 = NH2, R2 to R5 = H, R6 = OSO3H


42.
2α-carboxy, 4-carboxamido, butane-1-sulfonic acid
Z = NH2, R1, R3 to R6 = H, R2 = CH2SO3H


43.
2α-carboxy, 4-carboxamido, butane-1-sulfate
Z = NH2, R1, R3 to R6 = H, R2 = CH2OSO3H


44.
1α-carboxy, 3-carboxamido, propane-1-sulfonic acid
Z = NH2, R1, R3 to R6 = H, R2 = SO3H


45.
1α-carboxy, 3-carboxamido, propane-1-sulfate
Z = NH2, R1, R3 to R6 = H, R2 = OSO3H


46.
1β-carboxy, 3-carboxamido, propane-1-sulfate
Z = NH2, R2 to R6 = H, R1 = OSO3H


47.
1β-carboxy, 3-carboxamido, propane-1-sulfonic acid
Z = NH2, R2 to R6 = H, R1 = SO3H


48.
D-glutamine, N-sulfonic acid
Z = NH2, R2 = NHSO3H; R1 = H, R3 to R6 = H


49.
2β-carboxy, 4-carboxamido, butane-1-sulfonic acid
Z = NH2, R2 to R6 = H, R1 = CH2SO3H


50.
2β-carboxy, 4-carboxamido, butane-1-sulfate
Z = NH2, R2 to R6 = H, R1 = CH2OSO3H


51.
D-glutamine, 2α-sulfonic acid
Z = NH2, R2 = NH2, R3 to R6 = H, R1 = SO3H


52.
D-glutamine, 2α-sulfate
Z = NH2, R2 = NH2, R3 to R6 = H, R1 = OSO3H


53.
D-glutamine, 3α-sulfonic acid
Z = NH2, R2 = NH2, R1, R4 to R6 H, R3 = SO3H


54.
D-glutamine, 3α-sulfate
Z = R2 = NH2, R1, R4 to R6 H, R3 = OSO3H


55.
D-glutamine, 3β-sulfonic acid
Z = R2 = NH2, R1 = R3 = R5 = R6 = H, R4 = SO3H


56.
D-glutamine, 3β-sulfate
Z = R2 = NH2, R1 = R3 = R5 = R6 = H, R4 = OSO3H


57.
D-glutamine, 4α-sulfonic acid
Z = R2 = NH2, R1 = R3 = R4 = R6 = H, R5 = SO3H


58.
D-glutamine, 4α-sulfate
Z = R2 = NH2, R1 = R3 = R4 = R6 = H, R5 = OSO3H


59.
D-glutamine, 4β-sulfonic acid
Z = R2 = NH2, R1 = R3 = R4 = R5 = H, R6 = SO3H


60.
D-glutamine, 4β-sulfate
Z = R2 = NH2, R1 = R3 = R4 = R5 = H, R6 = OSO3H
















TABLE 3













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1.
L-homoglutamic acid, N-sulfonic acid
Z = OH, R1 = NHSO3H, R2 to R8 = H


2.
Pentane-2α, 5-dicarboxy-1-sulfonic acid
Z = OH, R1, R3 to R8 = H, R2 = CH2SO3H


3.
Pentane-2α, 5-dicarboxy-1-sulfate
Z = OH, R1, R3 to R8 = H, R2 = CH2OSO3H


4.
Butane-1α, 4-dicarboxy-1-sulfonic acid
Z = OH, R1, R3 to R8 = H, R2 = SO3H


5.
Butane-1α, 4-dicarboxy-1-sulfate
Z = OH, R1, R3 to R8 = H, R2 = OSO3H


6.
L-homoglutamic acid, 2β-sulfonic acid
Z = OH, R1 = NH2, R3 to R8 = H, R2 = SO3H


7.
L-homoglutamic acid, 2β-sulfate
Z = OH, R1 = NH2, R3 to R8 = H, R2 = OSO3H


8.
L-homoglutamic acid, 3α-sulfonic acid
Z = OH, R1 = NH2, R2 = H, R4 to R8 H, R3 = SO3H


9.
L-homoglutamic acid, 3α-sulfate
Z = OH, R1 = NH2, R2 = H, R4 to R8 H, R3 = OSO3H


10.
L-homoglutamic acid, 3β-sulfonic acid
Z = OH, R1 = NH2, R2 = R3 = H, R5 to R8 = H, R4 = SO3H


11.
L-homoglutamic acid, 3β-sulfate
Z = OH, R1 = NH2, R2 = R3 = H, R5 to R8 = H, R4 = OSO3H


12.
L-homoglutamic acid, 4α-sulfonic acid
Z = OH, R1 = NH2, R2 = R3 = R4 = H, R6 to R8 = H, R5 = SO3H


13.
L-homoglutamic acid, 4α-sulfate
Z = OH, R1 = NH2, R2 = R3 = R4 = H, R6 to R8 = H, R5 = OSO3H


14.
L-homoglutamic acid, 4β-sulfonic acid
Z = OH, R1 = NH2, R2 = R5 = H, R7 = R8 = H, R6 = SO3H


15.
L-homoglutamic acid, 4β-sulfate
Z = OH, R1 = NH2, R2 = R5 = H, R7 = R8 = H, R6 = OSO3H


16.
L-homoglutamic acid, 5α-sulfonic acid
Z = OH, R1 = NH2, R2 to R6 = H, R8 = H, R7 = SO3H


17.
L-homoglutamic acid, 5α-sulfate
Z = OH, R1 = NH2, R2 to R6 = H, R8 = H, R7 = OSO3H


18.
L-homoglutamic acid, 5β-sulfonic acid
Z = OH, R1 = NH2, R2 to R7 = H, R8 = SO3H


19.
L-homoglutamic acid, 5β-sulfate
Z = OH, R1 = NH2, R2 to R7 = H, R8 = OSO3H


20.
D-homoglutamic acid, N-sulfonic acid
Z = OH, R2 = NHSO3H, R1, R3 to R8 = H


21.
Pentane-2β, 5-dicarboxy-1-sulfonic acid
Z = OH, R2 to R8 = H, R1 = CH2SO3H


22.
Pentane-2β, 5-dicarboxy-1-sulfate
Z= OH, R2 to R8 = H, R1 = CH2OSO3H


23.
Butane-1β, 4-dicarboxy-1-sulfonic acid
Z = OH, R2 to R8 = H, R1 = SO3H


24.
Butane-1β, 4-dicarboxy-1-sulfate
Z = OH, R2 to R8 = H, R1 = OSO3H


25.
D-homoglutamic acid, 2α-sulfonic acid
Z = OH, R2 = NH2, R3 to R8 = H, R1 = SO3H


26.
D-homoglutamic acid, 2α-sulfate
Z = OH, R2 = NH2, R3 to R8 = H, R1 = OSO3H


27.
D-homoglutamic acid, 3α-sulfonic acid
Z = OH, R2 = NH2, R1, R4 to R8 H, R3 = SO3H


28.
D-homoglutamic acid, 3α-sulfate
Z = OH, R2 = NH2, R1, R4 to R8 H, R3 = OSO3H


29.
D-homoglutamic acid, 3β-sulfonic acid
Z = OH, R2 = NH2, R1 = R3 = H, R5 to R8 = H, R4 = SO3H


30.
D-homoglutamic acid, 3β-sulfate
Z = OH, R2 = NH2, R1 = R3 = H, R5 to R8 = H, R4 = OSO3H


31.
D-homoglutamic acid, 4α-sulfonic acid
Z = OH, R2 = NH2, R1 = R3 = R4 = H, R6 to R8 = H, R5 = SO3H


32.
D-homoglutamic acid, 4α-sulfate
Z = OH, R2 = NH2, R1 = R3 = R4 = H, R6 to R8 = H, R5 = OSO3H


33.
D-homoglutamic acid, 4β-sulfonic acid
Z = OH, R2 = NH2, R1 = H, R3 to R5 = H, R7 = R8 = H, R6 = SO3H


34.
D-homoglutamic acid, 4β-sulfate
Z = OH, R2 = NH2, R1 = H, R3 to R5 = H, R7 = R8 = H, R6 = OSO3H


35.
D-homoglutamic acid, 5α-sulfonic acid
Z = OH, R2 = NH2, R1 = R8 = H, R3 to R6 = H, R7 = SO3H


36.
D-homoglutamic acid, 5α-sulfate
Z = OH, R2 = NH2, R1 = R8 = H, R3 to R6 = H, R7 = OSO3H


37.
D-homoglutamic acid, 5β-sulfonic acid
Z = OH, R2 = NH2, R1 = H, R3 to R7 = H, R8 = SO3H


38.
D-homoglutamic acid, 5β-sulfate
Z = OH, R2 = NH2, R1 = H, R3 to R7 = H, R8 = OSO3H


39.
L-homoglutamine, N-sulfonic acid
Z = NH2, R1 = NHSO3H, R2 to R8 = H


40.
Pentane-2α-carboxy, 5-carboxamido-1-sulfonic acid
Z = NH2, R1 and R3 to R8 = H, R2 = CH2SO3H


41.
Pentane-2α-carboxy, 5-carboxamido-1-sulfate
Z = NH2, R1 and R3 to R8 = H, R2 = CH2OSO3H


42.
Butane-1α-carboxy, 4-carboxamido-1-sulfonic acid
Z = NH2, R1 and R3 to R8 = H, R2 = SO3H


43.
Butane-1α-carboxy, 4-carboxamido-1-sulfate
Z = NH2, R1 and R3 to R8 = H, R2 = OSO3H


44.
L-homoglutamine, 2β-sulfonic acid
Z = R1 = NH2, R3 to R8 = H, R2 = SO3H


45.
L-homoglutamine, 2β-sulfate
Z = R1 = NH2, R3 to R8 = H, R2 = OSO3H


46.
L-homoglutamine, 3α-sulfonic acid
Z = R1 = NH2, R2 = H, R4 to R8 = H, R3 = SO3H


47.
L-homoglutamine, 3α-sulfate
Z = R1 = NH2, R2 = H, R4 to R8 = H, R3 = OSO3H


48.
L-homoglutamine, 3β-sulfonic acid
Z = R1 = NH2, R2 = R3 = H, R5 to R8 = H, R4 = SO3H


49.
L-homoglutamine, 3β-sulfate
Z = R1 = NH2, R2 = R3 = H, R5 to R8 = H, R4 = OSO3H


50.
L-homoglutamine, 4α-sulfonic acid
Z = R1 = NH2, R2 = R3 = R4 = H, R6 to R8 = H, R5 = SO3H


51.
L-homoglutamine, 4α-sulfate
Z = R1 = NH2, R2 = R3 = R4 = H, R6 to R8 = H, R5 = OSO3H


52.
L-homoglutamine, 4β-sulfonic acid
Z = R1 = NH2, R2 = R5 = H, R7 = R8 = H, R6 = SO3H


53.
L-homoglutamine, 4β-sulfate
Z = R1 = NH2, R2 = R5 = H, R7 = R8 = H, R6 = OSO3H


54.
L-homoglutamine, 5α-sulfonic acid
Z = R1 = NH2, R2 to R6 = H, R8 = H, R7 = SO3H


55.
L-homoglutamine, 5α-sulfate
Z = R1 = NH2, R2 to R6 = H, R8 = H, R7 = OSO3H


56.
L-homoglutamine, 5β-sulfonic acid
Z = R1 = NH2, R2 to R7 = H, R8 = SO3H


57.
L-homoglutamine, 5β-sulfate
Z = R1 = NH2, R2 to R7 = H, R8 = OSO3H


58.
D-homoglutamine, N-sulfonic acid
Z = NH2, R2 = NHSO3H, R1 and R3 to R8 = H


59.
Pentane-2β-carboxy, 5-carboxamido-1-sulfonic acid
Z = NH2, R2 to R8 = H, R1 = CH2SO3H


60.
Pentane-2β-carboxy, 5-carboxamido-1-sulfate
Z = NH2, R2 to R8 = H, R1 = CH2OSO3H


61.
Butane-1β-carboxy, 4-carboxamido-1-sulfonic acid
Z = NH2, R2 to R8 = H, R1 = SO3H


62.
Butane-1β-carboxy, 4-carboxamido-1-sulfate
Z = NH2, R2 to R8 = H, R1 = OSO3H


63.
D-homoglutamine, 2α-sulfonic acid
Z = R2 = NH2, R3 to R8 H, R1 = SO3H


64.
D-homoglutamine, 2α-sulfate
Z = R2 = NH2, R3 to R8 H, R1 = OSO3H


65.
D-homoglutamine, 3α-sulfonic acid
Z = R2 = NH2, R1, R4 to R8 H, R3 = SO3H


66.
D-homoglutamine, 3α-sulfate
Z = R2 = NH2, R1, R4 to R8 H, R3 = OSO3H


67.
D-homoglutamine, 3β-sulfonic acid
Z = R2 = NH2, R1 = R3 = H, R5 to R8 = H, R4 = SO3H


68.
D-homoglutamine, 3β-sulfate
Z = R2 = NH2, R1 = R3 = H, R5 to R8 = H, R4 = OSO3H


69.
D-homoglutamine, 4α-sulfonic acid
Z = R2 = NH2, R1 = R3 = R4 = H, R6 to R8 = H, R5 = SO3H


70.
D-homoglutamine, 4α-sulfate
Z = R2 = NH2, R1 = R3 = R4 = H, R6 to R8 = H, R5 = OSO3H


71.
D-homoglutamine, 4β-sulfonic acid
Z = R2 = NH2, R1 = H, R3 to R5 = H, R7 = R8 = H, R6 = SO3H


72.
D-homoglutamine, 4β-sulfate
Z = R2 = NH2, R1 = H, R3 to R5 = H, R7 = R8 = H, R6 = OSO3H


73.
D-homoglutamine, 5α-sulfonic acid
Z = R2 = NH2, R1 = R8 = H, R3 to R6 = H, R7 = SO3H


74.
D-homoglutamine, 5α-sulfate
Z = R2 = NH2, R1 = R8 = H, R3 to R6 = H, R7 = OSO3H


75.
D-homoglutamine, 5β-sulfonic acid
Z = R2 = NH2, R1 = H, R3 to R7 = H, R8 = SO3H


76.
D-homoglutamine, 5β-sulfate
Z = R2 = NH2, R1 = H, R3 to R7 = H, R8 = OSO3H









REFERENCE EXAMPLE

The following reference example and examples illustrate the present invention but do not limit the present invention.


The solvents in the parenthesis show the developing and eluting solvents and the ratios of the solvent used are by volume in the chromatographic separation or TLC.


The solvents in the parenthesis in NMR show the solvents used in measurement.


REFERENCE EXAMPLE AND EXAMPLE

The following reference example and examples illustrate the present invention but do not limit the present invention. The solvents in the parenthesis show the developing and eluting solvents and the ratios of the solvent used are by volume in the chromatographic separation or TLC. The solvents in the parenthesis in NMR show the solvents used in measurement.


Reference Example 1

L-Glutamyl, N-Sulfonic Acid from Glutamic Acid Mono Tertiary Butyl Ester


Glutamic acid monotertiary butyl ester (1 eq.) was added portion-wise to a solution of SO2Cl2 (2 eq.) in dry CH2Cl2 at 0° C. followed by Et3N (3 eq.). Resulting solution stirred for 8 hrs at r. t. when TLC showed complete consumption of starting material. Solvent was evaporated and the crude was dried in vacuum. 3 ml water was added to it and the slurry was stirred for 1 hr. To the slurry was added 45 ml CH2Cl2 followed by 3 eq of TFA at 0° C. The resulting solution was stirred at r. t. for 24 hrs. The solvent was evaporated and dried in vacuum. The pseudo molecular ion, [M−H] at 226.0049 confirmed the structure of the product L-glutamyl, N-sulfonic acid (calculated for C5H8NO7S; 226.0026).


Reference Example 2

L-Glutamyl, N-Sulfonic Acid from Glutamic Acid Di Tertiary Butyl Ester


Glutamic acid ditertiary butyl ester (1 eq.) was added portion-wise to a solution of SO2Cl2 (2 eq.) in dry CH2Cl2 at 0° C. followed by Et3N (3 eq.). Resulting solution stirred for 8 hrs at r. t. when TLC showed complete consumption of starting material. Solvent was evaporated and the crude was dried in vacuum. 3 ml water was added to it and the slurry was stirred for 1 hr. To the slurry was added 45 ml CH2Cl2 followed by 3 eq of TFA at 0° C. The resulting solution was stirred at r. t. for 24 hrs. The solvent was evaporated and dried in vacuum. The pseudo molecular ion, [M−H] at 226.0049 confirmed the structure of the product L-glutamyl, N-sulfonic acid (calculated for C5H8NO7S; 226.0026).


Reference Example 3

L-Aspartyl, N-Sulfonic Acid from L-Aspartic Acid Di Tertiary Butyl Ester


L-aspartic acid di tertiary butyl ester (1 eq.) was added portion-wise to a solution of SO2Cl2 (2 eq.) in dry CH2Cl2 at 0° C. followed by Et3N (3 eq.). Result solution stirred for 8 hrs at r. t. when TLC showed complete consumption of starting material. Solvent was evaporated and the crude was dried in vacuum. 3 ml water was added to it and the slurry was stirred for 1 hr. To the slurry was added 45 ml CH2Cl2 followed by 3 eq of TFA at 0° C. The resulting solution was stirred at r. t. for 24 hrs. The solvent was evaporated and dried in vacuum. The pseudo molecular ion, [M−H] at 211.9885 confirmed the structure of the product L-aspartyl, N-sulfonic acid (calculated for C4H6NO7S; 211.9870).


Reference Example 4

L-Homoglutamyl, N-Sulfonic Acid from L-Homoglutamic Acid Di Tertiary Butyl Ester


L-Homoglutamic acid di tertiary butyl ester (1 eq.) was added portion-wise to a solution of SO2Cl2 (2 eq.) in dry CH2Cl2 at 0° C. followed by Et3N (3 eq.). Resulting solution stirred for 8 hrs at r. t. when TLC showed complete consumption of starting material. Solvent was evaporated and the crude was dried in vacuum. 3 ml water was added to it and the slurry was stirred for 1 hr. To the slurry was added 45 ml CH2Cl2 followed by 3 eq of TFA at 0° C. The resulting solution was stirred at r. t. for 24 hrs. The solvent was evaporated and dried in vacuum. The pseudo molecular ion, [M−H] at 240.0169 confirmed the structure of the product L-Homoglutamyl, N-sulfonic acid (calculated for C6H10NO7S; 240.0182).


Reference Example 5

The calcium salt of L-glutamyl-N-sulphonic acid was prepared by adding 1 M equivalent of CaCl2 solution and incubated at temperature ranging from 30±5° C. The resulting complex was freeze-dried. The freeze-dried compound was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table A).

TABLE AEffect of compound 1 (L-glutamyl-N-sulphonic acid, Ca salt) onosteoclast formationNumber of TRAP-positivemultinuclear cells/wellCultureof 96 well plateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL138.00 ± 9.37 M-CSF + RANKL +109.67 ± 9.79 21.01compound 1(0.5 μg/ml)M-CSF + RANKL +52.17 ± 6.42 62.19compound 1(1.5 μg/ml)M-CSF + RANKL +14.67 ± 1.98 89.36compound 1(3.0 μg/ml)M-CSF + RANKL +2.83 ± 1.0597.94compound 1(5.0 μg/ml)


Culture of murine bone marrow cells in the presence of M-CSF and RANKL induces the formation of osteoclasts, which were detected as TRAP-positive cells. A dose dependent inhibition in the number of osteoclast cells generated as observed with increasing dose of compound 1. Values given are the mean±SD of five separate experiments


Reference Example 6

The calcium salt of L-glutamic acid was prepared by adding 1 M equivalent of CaCl2 solution and incubated at temperature ranging from 30±5° C. The resulting complex was freeze-dried. The freeze-dried compound was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table B).

TABLE BEffect of L-glutamic acid, calcium salt on osteoclast formationNumber of TRAP-positivemultinuclear cells/well of 96 wellCultureplateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL158.33 ± 12.00M-CSF + RANKL +167.17 ± 7.95 0compound 2(0.5 μg/ml)M-CSF + RANKL +152.83 ± 10.473.47compound 2(1.5 μg/ml)M-CSF + RANKL +130.50 ± 13.5717.37compound 2(3.0 μg/ml)M-CSF + RANKL +119.50 ± 10.0024.52compound 2(5.0 μg/ml)
For detail see legend to example 5


Reference Example 7

The L-glutamyl-N-sulphonic acid prepared as described in Examples 1 & 2 was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table D).

TABLE-DEffect of L-glutamyl-N-sulphonic acid on osteoclast formationNumber of TRAP-positivemultinuclear cells/well of 96 wellCultureplateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL 146.83 ± 11.89M-CSF + RANKL +154.67 ± 8.430compound 3(0.5 μg/ml)M-CSF + RANKL +150.33 ± 8.820compound 3(1.5 μg/ml)M-CSF + RANKL +112.67 ± 8.6323.23compound 3(3.0 μg/ml)M-CSF + RANKL +110.00 ± 6.7225.08compound 3(5.0 μg/ml)
For detail see legend to example 5


Reference Example 8

The L-glutamic acid was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table E).

TABLE EEffect of L-glutamic acid on osteoclast formationNumber of TRAP-positivemultinuclear cells/well of 96 wellCultureplateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL156.00 ± 12.260M-CSF + RANKL +173.33 ± 6.50 0compound 4(0.5 μg/ml)M-CSF + RANKL +155.00 ± 8.23 0.64compound 4(1.5 μg/ml)M-CSF + RANKL +145.83 ± 14.717.05compound 4(3.0 μg/ml)M-CSF + RANKL +112.67 ± 10.7427.77compound 4(5.0 μg/ml)
For detail see legend to example 5


Reference Example 9

The L-Aspartic acid, N-sulphonic acid as prepared in example 3 was mixed with 1 M equivalent of CaCl2 solution and incubated at temperature ranging from 30±5° C. The resulting complex was freeze-dried. The freeze-dried compound was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table F).

TABLE FEffect of L-Aspartic acid, N-sulphonic acid calcium salt onosteoclast formationNumber of TRAP-positivemultinuclear cells/well of 96 wellCultureplateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL158.33 ± 11.260M-CSF + RANKL +127.30 ± 5.50 19.70compound 4(0.5 μg/ml)M-CSF + RANKL +86.23 ± 7.2345.16compound 4(1.5 μg/ml)M-CSF + RANKL +44.50 ± 4.8071.90compound 4(3.0 μg/ml)M-CSF + RANKL +26.67 ± 0.7383.26compound 4(5.0 μg/ml)
For detail see legend to example 5


Reference Example 10


L-homoglutamic acid, N-sulphonic acid as prepared in example 4 was mixed with 1 M equivalent of CaCl2 solution and incubated at temperature ranging from 30±5° C. The resulting complex was freeze-dried. The freeze-dried compound was reconstituted in sterilized distilled water and assessed in a dose-dependent manner for inhibition of osteoclast differentiation (Table G).

TABLE GEffect of L-homoglutamic acid, N-sulphonic acid, calcium salt onosteoclast formationNumber of TRAP-positivemultinuclear cells/well of 96 wellCultureplateconditions(Mean ± SEM)% inhibitionM-CSF0M-CSF + RANKL146..83 ± 12.00 M-CSF + RANKL +138.57 ± 7.95 5.55compound 2(0.5 μg/ml)M-CSF + RANKL +106.23 ± 10.4727.60compound 2(1.5 μg/ml)M-CSF + RANKL + 78.57 ± 13.5746.40compound 2(3.0 μg/ml)M-CSF + RANKL + 46.22 ± 10.0068.50compound 2(5.0 μg/ml)
For detail see legend to example 5


Reference Example 11

A. In Vitro Osteoclastogenesis Assay


For in vitro osteoclastogenesis bone marrow cells were isolated from 5-to 8-wk-old Balb/c mice. Mice were sacrificed by cervical dislocation and femora and tibiae were aseptically removed and dissected free of adherent soft tissues. The bone ends were cut, and the marrow cavity was flushed out with medium MEM from one end of the bone using a sterile 21-gauge needle. The bone marrow suspension was carefully agitated with a plastic Pasteur pipette to obtain a single-cell suspension. The cells were washed twice and resuspended (106 cells/ml) in {overscore (α)}MEM containing 10% FBS. Stromal cell-free, M-CSF-dependent, osteoclast precursor cells were prepared from these cells as previously described (Wani et al. 1999). Briefly, bone marrow cells were incubated for 24 h in {overscore (α)}MEM containing 10% FBS in the presence of M-CSF (10 ng/ml) at a density of 3×105 cells/ml in a 75 cm2 flask. After 24 h, nonadherent cells were harvested and layered on a Ficoll-Hypaque gradient. Cells at the gradient interface were collected, washed and resuspended (5×105/ml) in {overscore (α)}MEM containing 10% FBS. In this study, we called these stromal cell-free, M-CSF-dependent, nonadherent cells as osteoclast precursors. These osteoclast precursors were added to 96-well plates (100 μl/well) containing plastic coverslips. Each well received further 100 μl of medium containing M-CSF (30 ng/ml), RANKL (30 ng/ml) without or with various concentrations of purified compound. Cultures were fed every 2-3 days and after incubation for 6 days osteoclast formation was evaluated by tartrate-resistant acid phosphatase (TRAP) staining. The number of TRAP-positive multinucleated cells (MNCs) containing 3 or more nuclei was scored.


B. Characterization of Osteoclasts by TRAP Staining


Osteoclast formation was evaluated by quantification of TRAP-positive MNCs as described previously (Khapli et al. 2003). TRAP is preferentially expressed at high levels in osteoclast and is considered, especially in the mouse, to be an osteoclast marker. Cytochemical staining for TRAP is widely used for identifying the osteoclasts in vivo and in vitro. It is claimed to be specific for osteoclasts in bone. After incubation, cells on cover slips were washed in PBS, fixed in 10% formalin for 10 min and stained for acid phosphatase in the presence of 0.05 M sodium tartrate. The substrate used was napthol AS-BI phosphate. Only those cells that were strongly TRAP-positive (dark red) counted by light microscopy.


C. In Vitro Bone Resorption Assay


Osteoclast has the ability to excavate authentic resorption lacunae in vivo and in vitro. Bone resorption is the unique function of the osteoclast and is therefore the most useful means of distinguishing it from other cell types. M-CSF-dependent, non-adherent bone marrow cells were incubated for 10 days on bovine cortical bone slices in the presence of M-CSF, RANKL with or without various concentrations of compounds. Bone slices were examined for resorption pits by reflected light microscopy as previously described (Wani et al. 1999).


BRIEF DESCRIPTION OF THE ACCOMPANYING PLATES

Plate 1: Effect of compound as given in example 3 on RANKL-induced osteoclast differentiation from haemopoietic precursors of monocytes/macrophage lineage. Mice osteoclast precursors were incubated in the presence of M-CSF and RANKL in the absence and presence of the compound. Photomicrographs showing TRAP-positive osteoclasts in the absence (Plate 1A) and presence (Plate 1B) of the compound. This compound significantly inhibited osteoclast formation.


Plate 2: Effect of compound as described in example 4 on RANKL-induced osteoclast differentiation from haemopoietic precursors of monocytes/macrophage lineage. Photomicrographs showing TRAP-positive osteoclasts induced by M-CSF and RANKL in the absence (Plate 2A) and presence (Plate 2B) of the compound. This compound showed no inhibitory effect on osteoclast differentiation.

Claims
  • 1. A method for inhibiting osteoclast formation in a subject in need thereof, comprising the step of administering a pharmaceutically effective amount of a compound of the general formula ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the compound is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2, optionally with an additive, excipient, diluent or carrier.
  • 2. The method according to claim 1, for inhibiting formation of mononuclear TRAP-positive osteoclasts.
  • 3. The method according to claim 1, for inhibiting formation of multinuclear TRAP-positive osteoclasts.
  • 4. The method according to claim 1, wherein the pharmaceutically effective amount is 5 to 10 mg/kg of body weight.
  • 5. The method according to claim 1, comprising administering the compound for between 5 and 30 days.
  • 6. The method according to claim 1, comprising administering the compound for at least 30 days.
  • 7. The method according to claim 1, comprising administering the compound for at least 60 days.
  • 8. The method according to claim 1, comprising administering the compound for at least 90 days.
  • 9. The method according to claim 1, wherein the compound has the structure shown below and the formula ZOC—CR3R4—CR1R2—COOH, wherein one of R1 to R4 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2.
  • 10. The method according to claim 1, wherein the compound has the structure shown below and the formula ZOC—CR5R6—CR3R4—CR1R2—COOH, wherein one of R1 to R6 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2.
  • 11. The method according to claim 1, wherein the compound has the structure shown below and the formula ZOC—CR7R8—CR5R6—CR3R4—CR1R2—COOH, wherein one of R1 to R8 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2.
  • 12. The method according to claim 9, wherein the compound is selected from the group consisting of: I. L-Aspartic acid, N-Sulfonic acid, II. L-Aspartic acid, 2β-sulfonic acid, III. L-Aspartic acid, 2β-sulfate, IV. L-aspartic acid, 3α-sulfonic acid, V. L-aspartic acid, 3α-sulfate, VI. L-aspartic acid, 3β-sulfonic acid, VII. L-aspartic acid, 3β-sulfate, VIII. 2α,3-dicarboxy, propane-1-sulfonic acid, IX. 2α,3-dicarboxy, propane-1-sulfate, X. 1α,2-carboxy ethane sulfonic acid, XI. 1α,2-carboxy ethane sulfate, XII. D-aspartic acid, N-sulfonic acid, XIII. 2β,3-carboxy, propane-1-sulfonic acid, XIV. 2β,3-carboxy, propane-1-sulfate, XV. 1β,2-carboxy ethane-1-sulfonic acid, XVI. 1β,2-carboxy ethane-1-sulfate, XVII. D-aspartic acid, 2α-sulfonic acid, XVIII. D-aspartic acid, 2α-sulfonic acid, XIX. D-Aspartic acid, 3α-sulfonic acid, XX. D-Aspartic acid, 3α-sulfate, XXI. D-Aspartic acid, 3β-sulfonic acid, XXII. D-aspartic acid, 3β-sulfate, XXIII. L-asparagine, N-sulfonic acid, XXIV. 2α-carboxy, 3-carboxamido, propane-1-sulfonic acid, XXV. 2α-carboxy, 3-carboxamido, propane-1-sulfate, XXVI. 1α-carboxy, 2-carboxamido, ethane sulfonic acid, XXVII. 1α-carboxy, 2-carboxamido, ethane sulfate, XXVIII. L-asparagine, 2β-sulfonic acid, XXIX. L-asparagine, 2β-sulfate, XXX. L-asparagine, 3α-sulfonic acid, XXXI. L-asparagine, 3α-sulfate, XXXII. L-asparagine, 3β-sulfonic acid, XXXIII. L-asparagine, 3β-sulfate, XXXIV. D-asparagine, N-sulfonic acid, XXXV. 2β-carboxy, 3-carboxamido, propane-1-sulfonic acid, XXXVI. 2β-carboxy, 3-carboxamido, propane-1-sulfate, XXXVII. 1β-carboxy, 2-carboxamido, ethane sulfonic acid, XXXVIII. 1β-carboxy, 2-carboxamido, ethane sulfate, XXXIX. D-asparagine, 2α-sulfonic acid, XL. D-asparagine, 2α-sulfate, XLI. D-asparagine, 3α-sulfonic acid, XLII. D-asparagine, 3α-sulfate, XLIII. D-asparagine, 3β-sulfonic acid, XLIV. D-asparagine, 3β-sulfate.
  • 13. The method according to claim 10, wherein the compound is selected from the group consisting of: I. L-glutamic acid, N-sulfonic acid, II. 2α,4-dicarboxy, butane-1-sulfonic acid, III. 2α, 4-dicarboxy, butane-1-sulfate, IV. 1α,3-dicarboxy, propane sulfonic acid, V. 1α,3-dicarboxy, propane sulfate, VI. 1β,3-dicarboxy, propane sulfate, VII. 1β,3-dicarboxy, propane sulfonic acid, VIII. L-glutamic acid, 2β-sulfonic acid, IX. L-glutamic acid, 2β-sulfate, X. L-glutamic acid, 3α-sulfonic acid, XI. L-glutamic acid, 3α-sulfate, XII. L-glutamic acid, 3β-sulfonic acid, XIII. L-glutamic acid, 3β-sulfate, XIV. L-glutamic acid, 4α-sulfonic acid, XV. L-glutamic acid, 4α-sulfate, XVI. L-glutamic acid, 4β-sulfonic acid, XVII. L-glutamic acid, 4β-sulfate, XVIII. D-glutamic acid, N-sulfonic acid, XIX. 2β,4-dicarboxy, butane-1-sulfonic acid, XX. 2β,4-dicarboxy, butane-1-sulfate, XXI. D-glutamic acid, 2α-sulfonic acid, XXII. D-glutamic acid, 2α-sulfate, XXIII. D-glutamic acid, 3α-sulfonic acid, XXIV. D-glutamic acid, 3α-sulfate, XXV. D-glutamic acid, 3β-sulfonic acid, XXVI. D-glutamic acid, 3β-sulfate, XXVII. D-glutamic acid, 4α-sulfonic acid, XXVIII. D-glutamic acid, 4α-sulfate, XXIX. D-glutamic acid, 4β-sulfonic acid, XXX. D-glutamic acid, 4β-sulfate, XXXI. L-glutamine, N-sulfonic acid, XXXII. L-glutamine, 2β-sulfonic acid, XXXIII. L-glutamine, 2β-sulfate, XXXIV. L-glutamine, 3α-sulfonic acid, XXXV. L-glutamine, 3α-sulfate, XXXVI. L-glutamine, 3β-sulfonic acid, XXXVII. L-glutamine, 3β-sulfate, XXXVIII. L-glutamine, 4α-sulfonic acid, XXXIX. L-glutamine, 4α-sulfate, XL. L-glutamine, 4β-sulfonic acid, XLI. L-glutamine, 4β-sulfate, XLII. 2α-carboxy, 4-carboxamido, butane-1-sulfonic acid, XLIII. 2α-carboxy, 4-carboxamido, butane-1-sulfate, XLIV. 1α-carboxy, 3-carboxamido, propane-1-sulfonic acid, XLV. 1α-carboxy, 3-carboxamido, propane-1-sulfate, XLVI. 1β-carboxy, 3-carboxamido, propane-1-sulfate, XLVII. 1β-carboxy, 3-carboxamido, propane-1-sulfonic acid, XLVIII. D-glutamine, N-sulfonic acid, XLIX. 2β-carboxy, 4-carboxamido, butane-1-sulfonic acid, L. 2β-carboxy, 4-carboxamido, butane-1-sulfate, LI. D-glutamine, 2α-sulfonic acid, LII. D-glutamine, 2α-sulfate, LIII. D-glutamine, 3α-sulfonic acid, LIV. D-glutamine, 3α-sulfate, LV. D-glutamine, 3β-sulfonic acid, LVI. D-glutamine, 3β-sulfate, LVII. D-glutamine, 4α-sulfonic acid, LVIII. D-glutamine, 4α-sulfate, LIX. D-glutamine, 4β-sulfonic acid, LX. D-glutamine, 4β-sulfate.
  • 14. The method according to claim 11, wherein the compound is selected from the group consisting of: I. L-homoglutamic acid, N-sulfonic acid, II. Pentane-2α,5-dicarboxy-1-sulfonic acid, III. Pentane-2α,5-dicarboxy-1-sulfate, IV. Butane-1α,4-dicarboxy-1-sulfonic acid, V. Butane-1α,4-dicarboxy-1-sulfate, VI. L-homoglutamic acid, 2β-sulfonic acid, VII. L-homoglutamic acid, 2β-sulfate, VIII. L-homoglutamic acid, 3α-sulfonic acid, IX. L-homoglutamic acid, 3α-sulfate, X. L-homoglutamic acid, 3β-sulfonic acid, XI. L-homoglutamic acid, 3β-sulfate, XII. L-homoglutamic acid, 4α-sulfonic acid, XIII. L-homoglutamic acid, 4α-sulfate, XIV. L-homoglutamic acid, 4β-sulfonic acid, XV. L-homoglutamic acid, 4β-sulfate, XVI. L-homoglutamic acid, 5α-sulfonic acid, XVII. L-homoglutamic acid, 5α-sulfate, XVIII. L-homoglutamic acid, 5β-sulfonic acid, XIX. L-homoglutamic acid, 5β-sulfate, XX. D-homoglutamic acid, N-sulfonic acid, XXI. Pentane-2β,5-dicarboxy-1-sulfonic acid, XXII. Pentane-2β,5-dicarboxy-1-sulfate, XXIII. Butane-1β,4-dicarboxy-1-sulfonic acid, XXIV. Butane-1β,4-dicarboxy-1-sulfate, XXV. D-homoglutamic acid, 2α-sulfonic acid, XXVI. D-homoglutamic acid, 2α-sulfate, XXVII. D-homoglutamic acid, 3α-sulfonic acid, XXVIII. D-homoglutamic acid, 3α-sulfate, XXIX. D-homoglutamic acid, 3β-sulfonic acid, XXX. D-homoglutamic acid, 3β-sulfate, XXXI. D-homoglutamic acid, 4α-sulfonic acid, XXXII. D-homoglutamic acid, 4α-sulfate, XXXIII. D-homoglutamic acid, 4β-sulfonic acid, XXXIV. D-homoglutamic acid, 4β-sulfate, XXXV. D-homoglutamic acid, 5α-sulfonic acid, XXXVI. D-homoglutamic acid, 5α-sulfate, XXXVII. D-homoglutamic acid, 5β-sulfonic acid, XXXVIII. D-homoglutamic acid, 5β-sulfate, XXXIX. L-homoglutamine, N-sulfonic acid, XL. Pentane-2α-carboxy, 5-carboxamido-1-sulfonic acid, XYLI. Pentane-2α-carboxy, 5-carboxamido-1-sulfate, XLII. Butane-1α-carboxy, 4-carboxamido-1-sulfonic acid, XLIII. Butane-1α-carboxy, 4-carboxamido-1-sulfate, XLIV. L-homoglutamine, 2β-sulfonic acid, XLV. L-homoglutamine, 2β-sulfate, XLVI. L-homoglutamine, 3α-sulfonic acid, XLVII. L-homoglutamine, 3α-sulfate, XLVIII. L-homoglutamine, 3β-sulfonic acid, XLIX. L-homoglutamine, 3β-sulfate, L. L-homoglutamine, 4α-sulfonic acid, LI. L-homoglutamine, 4α-sulfate, LII. L-homoglutamine, 4β-sulfonic acid, LIII. L-homoglutamine, 4β-sulfate, LIV. L-homoglutamine, 5α-sulfonic acid, LV. L-homoglutamine, 5α-sulfate, LVI. L-homoglutamine, 5β-sulfonic acid, LVII. L-homoglutamine, 5β-sulfate, LVIII. D-homoglutamine, N-sulfonic acid, LIX. Pentane-2β-carboxy, 5-carboxamido-1-sulfonic acid, LX. Pentane-2β-carboxy, 5-carboxamido-1-sulfate, LXI. Butane-1β-carboxy, 4-carboxamido-1-sulfonic acid, LXII. Butane-1β-carboxy, 4-carboxamido-1-sulfate, LXIII. D-homoglutamine, 2α-sulfonic acid, LXIV. D-homoglutamine, 2α-sulfate, LXV. D-homoglutamine, 3α-sulfonic acid, LXVI. D-homoglutamine, 3α-sulfate, LXVII. D-homoglutamine, 3β-sulfonic acid, LXVIII. D-homoglutamine, 3β-sulfate, LXIX. D-homoglutamine, 4α-sulfonic acid, LXX. D-homoglutamine, 4α-sulfate, LXXI. D-homoglutamine, 4β-sulfonic acid, LXXII. D-homoglutamine, 4β-sulfate, LXXIII. D-homoglutamine, 5α-sulfonic acid, LXXIV. D-homoglutamine, 5α-sulfate, LXXV. D-homoglutamine, 5β-sulfonic acid, LXXVI. D-homoglutamine, 5β-sulfate.
  • 15. A method for treating osteoclasts in a subject in need of such treatment, comprising the step of administering a pharmaceutically effective amount of a compound of the general formula ZOC—(CRR)m—COOH, wherein: m=2, 3 or 4; Z is OH or NH2; one R in the compound is from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining Rs are H or NH2, optionally with an additive, excipient, diluent or carrier.
  • 16. The method according to claim 15, for treating mononuclear TRAP-positive osteoclasts.
  • 17. The method according to claim 15, for treating multinuclear TRAP-positive osteoclasts.
  • 18. The method according to claim 15, wherein the pharmaceutically effective amount is 5 to 10 mg/kg of body weight.
  • 19. The method according to claim 15, comprising administering the compound for between 5 and 30 days.
  • 20. The method according to claim 15, comprising administering the compound for at least 30 days.
  • 21. The method according to claim 15, comprising administering the compound for at least 60 days.
  • 22. The method according to claim 15, comprising administering the compound for at least 90 days.
  • 23. The method according to claim 15, wherein the compound has the structure shown below and the formula ZOC—CR3R4—CR1R2—COOH, wherein one of R1 to R4 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2.
  • 24. The method according to claim 15, wherein the compound has the structure shown below and the formula ZOC—CR5R6—CR3R4—CR1R2—COOH, wherein one of R1 to R6 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2.
  • 25. The method according to claim 15, wherein the compound has the structure shown below and the formula ZOC—CR7R8—CR5R6—CR3R4—CR1R2—COOH, wherein one of R1 to R8 is selected from the group consisting of SO3H, OSO3H, CH2—SO3H, CH2—OSO3H, and NHSO3H, and the remaining are H or NH2
  • 26. The method according to claim 23, wherein the compound is selected from the group consisting of: I. L-Aspartic acid, N-Sulfonic acid, II. L-Aspartic acid, 2β-sulfonic acid, III. L-Aspartic acid, 2β-sulfate, IV. L-aspartic acid, 3α-sulfonic acid, V. L-aspartic acid, 3α-sulfate, VI. L-aspartic acid, 3β-sulfonic acid, VII. L-aspartic acid, 3β-sulfate, VIII. 2α, 3-dicarboxy, propane-1-sulfonic acid, IX. 2α,3-dicarboxy, propane-1-sulfate, X. 1α,2-carboxy ethane sulfonic acid, XI. 1α,2-carboxy ethane sulfate, XII. D-aspartic acid, N-sulfonic acid, XIII. 2β,3-carboxy, propane-1-sulfonic acid, XIV. 2β,3-carboxy, propane-1-sulfate, XV. 1β,2-carboxy ethane-1-sulfonic acid, XVI. 1β,2-carboxy ethane-1-sulfate, XVII. D-aspartic acid, 2α-sulfonic acid, XVIII. D-aspartic acid, 2α-sulfonic acid, XIX. D-Aspartic acid, 3α-sulfonic acid, XX. D-Aspartic acid, 3α-sulfate, XXI. D-Aspartic acid, 3β-sulfonic acid, XXII. D-aspartic acid, 3β-sulfate, XXIII. L-asparagine, N-sulfonic acid, XXIV. 2α-carboxy, 3-carboxamido, propane-1-sulfonic acid, XXV. 2α-carboxy, 3-carboxamido, propane-1-sulfate, XXVI. 1α-carboxy, 2-carboxamido, ethane sulfonic acid, XXVII. 1α-carboxy, 2-carboxamido, ethane sulfate, XXVIII. L-asparagine, 2β-sulfonic acid, XXIX. L-asparagine, 2β-sulfate, XXX. L-asparagine, 3α-sulfonic acid, XXXI. L-asparagine, 3α-sulfate, XXXII. L-asparagine, 3β-sulfonic acid, XXXIII. L-asparagine, 3β-sulfate, XXXIV. D-asparagine, N-sulfonic acid, XXXV. 2β-carboxy, 3-carboxamido, propane-1-sulfonic acid, XXXVI. 2β-carboxy, 3-carboxamido, propane-1-sulfate, XXXVII. 1β-carboxy, 2-carboxamido, ethane sulfonic acid, XXXVIII. 1β-carboxy, 2-carboxamido, ethane sulfate, XXXIX. D-asparagine, 2α-sulfonic acid, XL. D-asparagine, 2α-sulfate, XLI. D-asparagine, 3α-sulfonic acid, XLII. D-asparagine, 3α-sulfate, XLIII. D-asparagine, 3β-sulfonic acid, XLIV. D-asparagine, 3β-sulfate.
  • 27. The method according to claim 24, wherein the compound is selected from the group consisting of: I. L-glutamic acid, N-sulfonic acid, II. 2α,4-dicarboxy, butane-1-sulfonic acid, III. 2α,4-dicarboxy, butane-1-sulfate, IV. 1α,3-dicarboxy, propane sulfonic acid, V. 1α,3-dicarboxy, propane sulfate, VI. 1β,3-dicarboxy, propane sulfate, VII. 1β,3-dicarboxy, propane sulfonic acid, VIII. L-glutamic acid, 2β-sulfonic acid, IX. L-glutamic acid, 2β-sulfate, X. L-glutamic acid, 3α-sulfonic acid, XI. L-glutamic acid, 3α-sulfate, XII. L-glutamic acid, 3β-sulfonic acid, XIII. L-glutamic acid, 3β-sulfate, XIV. L-glutamic acid, 4α-sulfonic acid, XV. L-glutamic acid, 4α-sulfate, XVI. L-glutamic acid, 4β-sulfonic acid, XVII. L-glutamic acid, 4β-sulfate, XVIII. D-glutamic acid, N-sulfonic acid, XIX. 2β,4-dicarboxy, butane-1-sulfonic acid, XX. 2β,4-dicarboxy, butane-1-sulfate, XXI. D-glutamic acid, 2α-sulfonic acid, XXII. D-glutamic acid, 2α-sulfate, XXIII. D-glutamic acid, 3α-sulfonic acid, XXIV. D-glutamic acid, 3α-sulfate, XXV. D-glutamic acid, 3β-sulfonic acid, XXVI. D-glutamic acid, 3β-sulfate, XXVII. D-glutamic acid, 4α-sulfonic acid, XXVIII. D-glutamic acid, 4α-sulfate, XXIX. D-glutamic acid, 4β-sulfonic acid, XXX. D-glutamic acid, 4β-sulfate, XXXI. L-glutamine, N-sulfonic acid, XXXII. L-glutamine, 2β-sulfonic acid, XXXIII. L-glutamine, 2β-sulfate, XXXIV. L-glutamine, 3α-sulfonic acid, XXXV. L-glutamine, 3α-sulfate, XXXVI. L-glutamine, 3β-sulfonic acid, XXXVII. L-glutamine, 3β-sulfate, XXXVIII. L-glutamine, 4α-sulfonic acid, XXXIX. L-glutamine, 4α-sulfate, XL. L-glutamine, 4β-sulfonic acid, XLI. L-glutamine, 4β-sulfate, XLII. 2α-carboxy, 4-carboxamido, butane-1-sulfonic acid, XLIII. 2α-carboxy, 4-carboxamido, butane-1-sulfate, XLIV. 1α-carboxy, 3-carboxamido, propane-1-sulfonic acid, XLV. 1α-carboxy, 3-carboxamido, propane-1-sulfate, XLVI. 1β-carboxy, 3-carboxamido, propane-1-sulfate, XLVII. 1β-carboxy, 3-carboxamido, propane-1-sulfonic acid, XLVIII. D-glutamine, N-sulfonic acid, XLIX. 2β-carboxy, 4-carboxamido, butane-1-sulfonic acid, L. 2β-carboxy, 4-carboxamido, butane-1-sulfate, LI. D-glutamine, 2α-sulfonic acid, LII. D-glutamine, 2α-sulfate, LIII. D-glutamine, 3α-sulfonic acid, LIV. D-glutamine, 3α-sulfate, LV. D-glutamine, 3β-sulfonic acid, LVI. D-glutamine, 3β-sulfate, LVII. D-glutamine, 4α-sulfonic acid, LVIII. D-glutamine, 4α-sulfate, LIX. D-glutamine, 4β-sulfonic acid, LX. D-glutamine, 4β-sulfate.
  • 28. The method according to claim 25, wherein the compound is selected from the group consisting of: I. L-homoglutamic acid, N-sulfonic acid, II. Pentane-2α,5-dicarboxy-1-sulfonic acid, III. Pentane-2α,5-dicarboxy-1-sulfate, IV. Butane-1α,4-dicarboxy-1-sulfonic acid, V. Butane-1α,4-dicarboxy-1-sulfate, VI. L-homoglutamic acid, 2β-sulfonic acid, VII. L-homoglutamic acid, 2β-sulfate, VIII. L-homoglutamic acid, 3α-sulfonic acid, IX. L-homoglutamic acid, 3α-sulfate, X. L-homoglutamic acid, 3β-sulfonic acid, XI. L-homoglutamic acid, 3β-sulfate, XII. L-homoglutamic acid, 4α-sulfonic acid, XIII. L-homoglutamic acid, 4α-sulfate, XIV. L-homoglutamic acid, 4β-sulfonic acid, XV. L-homoglutamic acid, 4β-sulfate, XVI. L-homoglutamic acid, 5α-sulfonic acid, XVII. L-homoglutamic acid, 5α-sulfate, XVIII. L-homoglutamic acid, 5β-sulfonic acid, XIX. L-homoglutamic acid, 5β-sulfate, XX. D-homoglutamic acid, N-sulfonic acid, XXI: Pentane-2α,5-dicarboxy-1-sulfonic acid, XXII. Pentane-2α,5-dicarboxy-1-sulfate, XXIII. Butane-1β,4-dicarboxy-1-sulfonic acid, XXIV. Butane-1β,4-dicarboxy-1-sulfate, XXV. D-homoglutamic acid, 2α-sulfonic acid, XXVI. D-homoglutamic acid, 2α-sulfate, XXVII. D-homoglutamic acid, 3α-sulfonic acid, XXVIII. D-homoglutamic acid, 3α-sulfate, XXIX. D-homoglutamic acid, 3β-sulfonic acid, XXX. D-homoglutamic acid, 3β-sulfate, XXXI. D-homoglutamic acid, 4α-sulfonic acid, XXXII. D-homoglutamic acid, 4α-sulfate, XXXIII. D-homoglutamic acid, 4β-sulfonic acid, XXXIV. D-homoglutamic acid, 4β-sulfate, XXXV. D-homoglutamic acid, 5α-sulfonic acid, XXXVI. D-homoglutamic acid, 5α-sulfate, XXXVII. D-homoglutamic acid, 5β-sulfonic acid, XXXVIII. D-homoglutamic acid, 5β-sulfate, XXXIX. L-homoglutamine, N-sulfonic acid, XL. Pentane-2α-carboxy, 5-carboxamido-1-sulfonic acid, X LI. Pentane-2α-carboxy, 5-carboxamido-1-sulfate, XLII. Butane-1α-carboxy, 4-carboxamido-1-sulfonic acid, XLIII. Butane-1α-carboxy, 4-carboxamido-1-sulfate, XLIV. L-homoglutamine, 2β-sulfonic acid, XLV. L-homoglutamine, 2β-sulfate, XLVI. L-homoglutamine, 3α-sulfonic acid, XLVII. L-homoglutamine, 3α-sulfate, XLVIII. L-homoglutamine, 3β-sulfonic acid, XLIX. L-homoglutamine, 3β-sulfate, L. L-homoglutamine, 4α-sulfonic acid, LI. L-homoglutamine, 4α-sulfate, LII. L-homoglutamine, 4β-sulfonic acid, LIII. L-homoglutamine, 4β-sulfate, LIV. L-homoglutamine, 5α-sulfonic acid, LV. L-homoglutamine, 5α-sulfate, LVI. L-homoglutamine, 5β-sulfonic acid, LVII. L-homoglutamine, 5β-sulfate, LVIII. D-homoglutamine, N-sulfonic acid, LIX. Pentane-2β-carboxy, 5-carboxamido-1-sulfonic acid, LX. Pentane-2β-carboxy, 5-carboxamido-1-sulfate, LXI. Butane-1β-carboxy, 4-carboxamido-1-sulfonic acid, LXII. Butane-1β-carboxy, 4-carboxamido-1-sulfate, LXIII. D-homoglutamine, 2α-sulfonic acid, LXIV. D-homoglutamine, 2α-sulfate, LXV. D-homoglutamine, 3α-sulfonic acid, LXVI. D-homoglutamine, 3α-sulfate, LXVII. D-homoglutamine, 3β-sulfonic acid, LXVIII. D-homoglutamine, 3β-sulfate, LXIX. D-homoglutamine, 4α-sulfonic acid, LXX. D-homoglutamine, 4α-sulfate, LXXI. D-homoglutamine, 4β-sulfonic acid, LXXII. D-homoglutamine, 4β-sulfate, LXXIII. D-homoglutamine, 5α-sulfonic acid, LXXIV. D-homoglutamine, 5α-sulfate, LXXV. D-homoglutamine, 5β-sulfonic acid, LXXVI. D-homoglutamine, 5β-sulfate.
RELATED APPLICATIONS

This application claims priority to U.S. Provisional Patent Application No. 60/512,183, filed Oct. 20, 2003, whose contents are incorporated by reference.

Provisional Applications (1)
Number Date Country
60512183 Oct 2003 US