Claims
- 1. A method of treating a mammal having a need for, or a reduced ability to produce, insulin, comprising administering to said mammal an insulin having an in vitro activity of stimulating glucose incorporation into glycogen in rat soleus muscle, and an amylin having the in vitro activity of suppressing glucose incorporation into glycogen in rat soleus muscle, wherein said insulin and said amylin are administered in a molar ratio of between about 1:1 and about 67:1.
- 2. A method according to claim 1 wherein said method further comprises identifying a mammal having a reduced ability to produce serum insulin compared to the normal ability of said mammal to produce serum insulin.
- 3. A method according to claim 1 wherein said insulin and said amylin are administered in a molar ratio of between about 7:1 and about 67:1.
- 4. A method according to claim 1 wherein said insulin and said amylin are administered in a molar ratio of between about 1:1 to about 40:1.
- 5. A method according to claim 4 wherein said insulin and said amylin are administered in a molar ratio of between about 2.5:1 and about 35:1.
- 6. A method according to claim 4 wherein said insulin and said amylin are administered in a molar ratio of between about 5:1 and about 25:1.
- 7. A method according to claim 4 wherein said insulin and said amylin are administered in a molar ratio of between about 5:1 and about 10:1.
- 8. A method according to claim 4 wherein said insulin and said amylin are administered in a molar ratio of between about 4:1 and about 10:1.
- 9. A method according any of claims 1-8 wherein said amylin comprises .sup.25,28,29 Pro-human amylin.
- 10. A method according to any of claims 1-5 or 6-8 wherein said amylin comprises .sup.25,28,29 Pro-human amylin and said mammal is a human.
- 11. A method according to any of claims 1-5 or 6-8 wherein said amylin comprises .sup.25,28,29 Pro-human amylin and said mammal is a human suffering from diabetes mellitus.
- 12. A method according to claim 1 wherein said amylin is administered in an amount of at least about 0.2 micrograms per kilogram of said mammal per day.
- 13. A method according to claim 1 wherein said amylin is administered in an amount of at least about 0.4 micrograms per kilogram of said mammal per day.
- 14. A method according to claim 1 wherein said amylin is administered in an amount of at least about 0.5 micrograms per kilogram of said mammal per day.
- 15. A method according to claim 1 wherein said amylin is administered in an amount of between about 0.2 micrograms per kilogram of said mammal per day and about 5 micrograms per kilogram of said mammal per day.
- 16. A method according to claim 15 wherein said amylin is administered in an amount of between about 0.4 micrograms per kilogram of said mammals per day and about 2 micrograms per kilogram of said mammal per day.
- 17. A method according to any of claims 12-16 wherein said amylin comprises .sup.25,28,29 Pro-human amylin.
- 18. A method according to any of claims 12-16 wherein said amylin comprises 25,28,29Pro-human amylin and said mammal is a human.
- 19. A method according to any of claims 12-16 wherein said amylin comprises .sup.25,28,29 Pro-human amylin and said mammal is a human suffering from diabetes.
- 20. A method according to claim 1 wherein said mammal is a human suffering from diabetes and said amylin is administered in an amount of at least about 90 micrograms per day.
- 21. A method according to claim 20 wherein said amylin comprises .sup.25,28,29 Pro-human amylin.
- 22. A method of treating a mammal having a need for, or a reduced ability to produce, insulin, comprising administering to said mammal a composition comprising an insulin having an in vitro activity of stimulating glucose incorporation into glycogen in rat soleus muscle, and an amylin having the in vitro activity of suppressing glucose incorporation into glycogen in rat soleus muscle, wherein said insulin and said amylin are administered in a suitable molar ratio such that, upon administration of such composition to said mammal, the amount of said amylin said composition will result in plasma levels of amylin of about 3% to about 6% the plasma levels of insulin.
- 23. A method of treating a mammal having a need for, or a reduced ability to produce, insulin, comprising administering to said mammal a composition comprising an insulin having an in vitro activity of stimulating glucose incorporation into glycogen in rat soleus muscle, and an amylin having the in vitro activity of suppressing glucose incorporation into glycogen in rat soleus muscle, wherein said insulin and said amylin are administered in a suitable molar ratio such that, upon administration of such composition to said mammal, the amount of said amylin in said composition will result in plasma levels of amylin of about 4% to about 7% the plasma levels of insulin.
- 24. A method according to either of claims 22 or 23 wherein said amylin comprises .sup.25,28,29 Pro-human amylin and said mammal is a human.
- 25. A composition comprising an insulin having an in vitro activity of stimulating glucose incorporation into glycogen in rat soleus muscle, and an amylin having the in vitro activity of suppressing glucose incorporation into glycogen in rat soleus muscle, wherein said insulin and said amylin are provided in a molar ratio of between about 1:1 and about 67:1.
- 26. A composition according to claim 25 wherein said insulin and said amylin are provided at a molar ratio of between about 7:1 and about 67:1.
- 27. A composition according to claim 25 wherein said insulin and said amylin are provided at a molar ratio of between about 1:1 to about 40:1.
- 28. A composition according to claim 27 wherein said insulin and said amylin are provided at a molar ratio of between about 2.5:1 and about 35:1.
- 29. A composition according to claim 27 wherein said insulin and said amylin are provided at a molar ratio of between about 5:1 and about 25:1.
- 30. A composition according to claim 27 wherein said insulin and said amylin are provided at a molar ratio of between about 5:1 and about 10:1.
- 31. A composition according to claim 27 wherein said insulin and said amylin are provided at a molar ratio of between about 4:1 and about 10:1.
- 32. A composition according to any of claims 25-31 wherein said amylin comprises .sup.25,28,29 Pro-human amylin.
RELATED APPLICATIONS
This application is a continuation-in-part of application Ser. No. 08/127,705 filed Sep. 27, 1993 which is a continuation of application Ser. No. 07/704,995 filed May 24, 1991 both abandoned.
US Referenced Citations (5)
Foreign Referenced Citations (1)
Number |
Date |
Country |
0309100 |
Mar 1989 |
EPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
704995 |
May 1991 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
127705 |
Sep 1993 |
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