Claims
- 1. A colorimetric method for quantitative assay of catechol or a catechol generating substance, comprising the steps of:
- a) admixing a sample suspected of containing catechol or a catechol generating substance with NADH, tyrosinase, and a dye selected from the group consisting of diarylimidazoles, triarylimidazoles, triarylmethanes, and reduced indophenols; and
- b) determining the quantity of catechol present in the sample by colorimetric measurement.
- 2. The method of claim 1 wherein step a) further includes an enzyme for the conversion of the catechol generating substance to catechol.
- 3. The method of claim 1 wherein the catechol generating substance is selected from the group consisting of o-hydroxyphenyl phosphate and tyrosine o-phosphate.
- 4. A colorimetric method for a quantitative assay of salicylate comprising the steps of:
- a) admixing a sample suspected of containing salicylate with, in any order or combination, NADH, salicylate hydroxylase, tyrosinase, and a dye selected from the group consisting of diarylimidizoles, triarylimidizoles, triarylmethanes, and reduced indophenols; and
- b) determining the quantity of salicylate present in the sample by colorimetric measurement.
- 5. The method of claim 4 wherein said dye is 2-(4-hydroxy-3,5-dimethoxyphenyl)-4,5-bis(4-dimethylaminophenyl)imidazole.
- 6. The method of claim 4 wherein the NADH is 1 to 2 mM/mL.
- 7. The method of claim 4 wherein the salicylate hydroxylase is 20 to 300 U/mL.
- 8. The method of claim 4 wherein the tyrosinase is 50 to 1000 U/mL.
- 9. The method of claim 4 wherein the dye is 0.2 to 10 .mu.g/mL.
- 10. The method of claim 4 wherein said assay is conducted at a pH range of 6 to 8.5.
Parent Case Info
This is a Divisional of application Ser. No. 548,395, filed 05 Jul. 1990, now U.S. Pat. No. 5,320,946.
US Referenced Citations (4)
Non-Patent Literature Citations (2)
Entry |
Webster's Third New International Dictionary, p. 1107 & p. 1299, (1986). |
Clin. Chem., 30:1549, 1984 You et al. |
Divisions (1)
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Number |
Date |
Country |
Parent |
548395 |
Jul 1990 |
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