Claims
- 1. In a method of assaying for ATP which comprises providing a solution containing intracellular ATP and a cationic surfactant for extracting the ATP, adding to the solution an ATP-utilizing enzyme, and measuring the activity of the enzyme, the improvement which comprises contacting the solution, prior to or simultaneous with the addition thereto of the ATP-utilizing enzyme, with a cyclodextrin or a cyclodextrin derivative of an appropriate type and in an appropriate amount to neutralize the cationic surfactant.
- 2. The method as claimed in claim 1, wherein the enzymatic assay is performed in the presence of the cyclodextrin or derivative.
- 3. The method as claimed in claim 1, wherein the enzyme is firefly luciferase.
- 4. The method as claimed in claim 3, wherein the enzymatic assay is performed in the presence of the cyclodextrin or derivative.
- 5. A kit for the extraction and assay of ATP in biological specimens by the method of claim 1, wherein the kit comprises the following components:
- a) an extracting substance comprising cationic surfactant,
- b) a cyclodextrin,
- c) a firefly luciferase, and
- d) an assay buffer.
- 6. The kit as claimed in claim 5, wherein:
- the extracting substance is provided in a form dried on or in a matrix that picks up a sample of suitable size when contacted with a fluid specimen;
- the cyclodextrin is provided in a form dissolved in the assay buffer;
- the firefly luciferase reagent is provided form dried on or in a matrix that can release the reagent the assay buffer.
Priority Claims (1)
Number |
Date |
Country |
Kind |
9100551 |
Jan 1991 |
GBX |
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Parent Case Info
This application is a continuation of now abandoned application, Ser. No. 08/075,484, filed Jun. 11, 1993 which is the U.S. National Stage application of PCT/GB92/00056, filed Jan. 10, 1992, published as WO92/12253, Jul. 23, 1992.
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Continuations (1)
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Number |
Date |
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Parent |
75484 |
Jun 1993 |
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