Patent Grant
7351861
The present invention relates to a method of assessing bacterial virulence modifying properties of chemical compounds and other agents including mixtures of agents, to a corresponding probe, to compounds possessing such properties identified by the method, and to their use.
The use of powerful anti-microbial agents in combination with improved living conditions dramatically increased our capacity to fight off previously lethal infections. However, in the last ten years there has been a dramatic escalation in antibiotic resistance among microorganisms1,2. Such resistance proliferates readily in the bacterial kingdom through gene transfer, making the spread of resistance hard to control. Although a multitude of efforts to improve the situation have been made it is clear that additional approaches for microbe control are required3. One obvious way to address this challenge is to develop novel anti-microbial drugs. This task is however arduous and most antibacterial agents that have reached the market during the last decade are based on previously characterized structures with known modes of action i.e. a bactericidal or static effect. Recent advances in combinatorial chemistry4-6, genomics7,8, and screening technologies9,10 however increase our capacity to identify novel bacterial targets and compounds that interfere with them. Combinatorial chemistry can indeed generate large numbers of diverse compounds but screening for novel agents from such libraries still mainly focus on agents that target microbial growth essentially in analogy with existing drugs11. An alternative is to identify compounds with a novel mode of action that target microbial virulence rather than growth12,13. Virulence includes events that enable the bacterium to enter the host, disarm the host's defence, multiply, and finally spread within the host or to a new host. Agents that target virulence are potentially effective antimicrobials but also apply less selective pressure for resistance. Moreover, compounds that perturb a virulence system can be employed as chemical probes to elucidate unknown features of bacterial virulence using a chemical genetics approach14,15.
Recent studies have revealed that various pathogenic bacteria use related virulence systems, findings that contradict the long held paradigm that each bacterium has a unique mode of action. The type III secretion system of Yersinia represents the archetype of one of these systems in which the bacteria adhere to eukaryotic cells and inject a set of bacterial effector proteins, Yops (yersinia outer proteins), that are capable of subverting the target cell16,17. This process involves a secretion of the Yops across the bacterial membranes and a subsequent translocation across the eukaryotic cell membrane. The genus Yersinia includes eleven known species of which Y. pestis, Y. speudotuberculosis, and Y. enterocolitica are pathogenic to humans of which Y. pestis, the causative agent of plague, is one of the most virulent bacteria known to man. These three species all share the tropism for lymphoid tissue and a capacity to evade the non-specific immune response. The processes of protein secretion and translocation represent attractive points of attack for novel antibacterial agents. The secretion apparatus is essential for the bacteria to evade the immune defence and it is possible that agents that inhibit the secretion can result in an antibacterial response without actually killing the bacteria. Moreover, several mammalian pathogens including Yersinia spp., Salmonella spp., Shigella flexneri, Pseudomonas aureginosa, enteropathogenic Escherichia coli, Chlamydia spp., and also plant pathogens like Xanthomonas campestris, Erwinia spp., Pseudomonas syringae, and Ralstonia solanacearum employ type III secretion systems that are crucial for virulence18,19. Some components of type III secretion systems in different species are interchangeable, which suggests evolutionary conservation and that data generated with one genus might also be valid for others. Thus, the type III secretion in gram negative bacteria is an important virulence system that constitutes an attractive drug target as well as a challenge for chemical genetics. The relevance of type III secretion in basic research and drug development is further stressed by the fact that multi-resistance strains have been found in Y. pestis20,21 and that Y. pestis is a potential weapon in biological warfare and bioterrorism22,23.
U.S. Pat. No. 6,136,542 discloses a method for screening agents that activate or inhibit type II secretion machinery in gram-negative bacteria. The method comprises exposing gram-negative bacterial cells to a sample of an agent to be screened, which cells contain a reporter gene, such as the luxAB gene, transcriptionally fused to a promoter of a gene activated or regulated by the type III secretion machinery, and detecting the presence or activity of the product of the reporter gene. The detection indicates whether the sample activates or inhibits type III secretion machinery. The gram-negative bacteria is selected from, i.a., Yersinia.
It is an object of the present invention to provide to a method for assessing bacterial virulence modifying properties of chemical compounds and other agents including mixtures of agents.
It is another object of the invention to provide a corresponding probe.
A further object of the invention is to provide agents capable of decreasing bacterial virulence.
Additional objects of the invention will become evident from the following description of the invention, a drawing comprising a number of figures, the description of preferred embodiments, and the appended claims.
According to the present invention is disclosed a method of identifying antibacterial agents. The method of the invention is based on a Yersinia pseudotuberculosis assay. It can be used to identify agents that target known or unknown components directly or indirectly, affecting the type III secretion machinery.
Thus, according to the invention is disclosed a method of screening for agents inhibiting or activating bacterial virulence, the method being carried out in absence of eukaryotic cells, comprising contacting a gram-negative bacterium culture depleted in Ca2+, in particular a Yersinia species, comprising a luxAB reporter gene construct, with a potentially bacterial virulence inhibiting or activating agent, thereby forming a test suspension, manipulating the temperature of the test suspension and adding an aliphatic aldehyde to make the bacterium emit light, measuring the emitted light, comparing the amount of emitted light with the light emitted in absence of the bacterial virulence inhibiting or activating agent.
The method of the invention for identifying antibacterial agents comprises depleting bacteria of a strain comprising a luxAB construct of Ca2+, incubating the Ca2+ depleted bacteria with an agent the antibacterial effect of which shall be determined, recording the light emitted by the bacteria upon addition of an aldehyde such as decanal, the incubation being carried out at a temperature which is at least 10° C. higher than the temperature at which the light is emitted by the bacteria, preferably at least 15° C. higher. It is preferred for the strain to be a Yersinia sp. strain, in particular a Yersinia pseudotuberculosis strain. Preferred incubation and emission temperatures are about 37° C. and about 21° C., respectively. Agents having a useful antibacterial effect will inhibit, at a concentration of 50 μM, the luciferase light signal from pIB29EL by at least 40%, more preferred by at least 60%, most preferred by at least 80%.
More specifically, the method of the invention comprises the following steps:
Preferably the first and third temperature is from 20° C. to 26° C., most preferred about 21° C., while the second temperature is about 37° C. Decanal or a functionally equivalent aldehyde is preferably added to the test suspension in form of an aqueous emulsion. At the first hand, the compound to be tested or a salt thereof is dissolved in water. If its solubility in water is to low, it may be dissolved in an organic solvent, preferably DMSO, or a mixture of water and an organic solvent. Preferred concentrations of test compound in the test suspension are from 10 μg per mL to 100 μg per mL.
It is, of course, also possible to identify compounds that increase the level of virulence factors (activators) by the method of the invention which has to be slightly modified by substituting the Ca2+ depleted medium by a medium which is not Ca2+ depleted. An activator will counteract the inhibition of the type III secretion machinery by LcrQ and provide for light emission which can be measured.
According to a preferred aspect of the invention the bacterial strain comprising the luxAB construct is a Yersinia pseudotuberculosis strain or a mutant strain thereof, in particular a non-virulent strain, such as the pIB29EL strain. Other useful strains are pIB29AL, pIB102EL, pIB102AL, pIB102FL, and pIB102FΔhlhL.
According to the invention there is thus disclosed a probe for identifying antibacterial agents comprising the Yersinia pseudotuberculosis strain pIB29EL. According to the invention there is also disclosed a probe for identifying antibacterial agents comprising the Yersinia pseudotuberculosis strains pIB29AL, pIB102EL, pIB102AL. Other probes according to the invention for identifying antibacterial agents comprise the Yersinia pseudotuberculosis strains pIB102FL and pIB102FΔhlhL.
Regulation of the type III secretion machinery in Y. pseudotuberculosis is relatively well understood. Its modest virulence compared to Y. pestis makes it a suitable candidate for assay development. In addition, it was recently established that Y. pestis has evolved from Y. pseudotuberculosis. The components of the virulence system, i.e. the effector proteins and proteins involved in regulation and secretion, are encoded by a −70 kb virulence plasmid. Although many functions of the Yersinia type III secretion and its regulation are poorly understood, results from different laboratories have contributed to provide a broad picture of secretion-related events16,18,19. When the bacteria enter the host and sense a temperature shift to 37° C. they prepare for battle by producing −20 Ysc (yersinia secretion) proteins that form the secretion channel spanning the bacterial inner and outer membranes. This temperature shift also results in expression of chaperones, Sycs (specific yop chaperones) which, at a later stage, protect the Yersinia outer proteins, Yops, and deliver them to the Ysc apparatus. Expression of the Ysc proteins and Sycs is regulated by the temperature triggered protein LcrF. On the other hand, low LcrQ suppress the expression of the Yops, which thus are present in small amounts only, until the bacterium comes in direct contact with a eukaryotic cell. Contact with β-integrin on the eukaryotic target cell triggers the secretion of LcrQ which results in strong Yop production. The cognate Sycs then bind to the newly produced Yops and deliver them to the Ysc apparatus. In parallel a poorly understood chain of events results in formation of a pore in the eukaryotic cell membrane. The structure of this pore is unknown but several Yersinia proteins including LcrV, YscF, YopB, and YopD are known to be crucial for this process. The Yops are secreted through the Ysc machinery and then translocated through the pore into the cytoplasm of the eukaryotic cell. In the eukaryotic cell the six different Yops, YopE, YopH, YpkA, YopJ, YopM, and YopT specifically target eukaryotic processes such as phagocytosis and production of proinflammatory cytokines. The production and secretion of Yops have been found to be Ca2+ dependent in vitro. At 26° C. Yersinia grows both in the presence and absence of Ca2+, whereas millimolar concentrations of Ca2+ are required for growth at 37° C. In Ca2+ depleted medium at 37° C. a metabolic downshift is observed and growth is halted after a few generations. Most important, this is accompanied by LcrQ secretion and subsequent strong expression and secretion of the Yops in absence of eukaryotic cells. Ca2+ depletion in vitro thus mimicks external stimuli that the bacteria encounter in the host. The role of this Ca2+ dependence in vivo has however not been clarified. The simplified regulatory model for Syc and Yop expression given in
In a general procedure an over-night culture is diluted and dispensed in, for instance, 96-well plates. After initial incubation at room temperature which allows the culture to attain stable growth, the temperature of the plate including the wells is brought to 37° C. In the absence of Ca2+ growth is suppressed and secretion is activated whereas, in the presence of Ca2+, secretion remains at a inactive level while the bacteria continue to grow. The plate is then cooled to room temperature, that is, about 21° C., and kept at that temperature for a selected period of time, after which n-decanal is added and the emission of light is measured with a camera. The assay conditions including initial bacterial density, incubation times, etc. have been optimised (data not shown) to give a robust and reliable system which is useful for the identification of secretion inhibitors. A typical result from an assay with bacteria alone (pIB29EL) in absence or presence of Ca2+ is shown in
Since the assay is based on a transcriptional readout, agents perturbing not only the type III secretion system have the potential to result in reduction of light emission. To address the scope and limitations of the assay a number of well-studied antibiotics with known modes of actions were evaluated. As summarized in Table 1 carbenicillin, streptomycin, nalidixic acid, and polymyxin B all result in a dose-dependent inhibition of light-emission with the indicated IC50 values. These data clearly illustrate that the assay; despite that only a short period of time is allowed for growth (<3 h), efficiently can detect bactericidal agents acting on a variety of bacterial targets.
According to a preferred aspect the method of the invention can also be used to screen for agents specifically targeting the type III secretion system. A panel of polyclonal rabbit serum antibodies against a set of relevant Yersinia proteins that putatively are present on the bacterial surface and directly or indirectly regulate the secretion was evaluated. The rabbit serum was however found to contain one or more factors that enhanced light-emission both in presence and absence of Ca2+ (data not shown). In spite of that antibodies against YopB were capable of completely inhibiting light-emission (data not shown). YopB is a Yersinia outer protein believed to be involved in formation of pores in the eukaryotic cell membrane. To further study this inhibitory effect a monoclonal mouse antibody against YopB was tested and found to abrogate light emission when added to the assay solution up to 2 h after start of the experiment (
The assay of the invention can be used to screen for agents targeting many different bacterial functions. In the first place the assay allows compounds that interfere with the type III secretion system to be detected. The assay is well suited for high-throughput screening of large compound collections. Compounds that interfere with the secretion system have potential both as drug candidates and molecular probes for functional studies of the type III secretion system in Yersinia and other gram-negative bacteria. Furthermore, a powerful feature of this assay is that it also can be used to identify activators of the system. Such compounds will be useful for investigations aimed at understanding the regulation of the system. Screening of a 9,400 compound library by the method identified a number of compounds which inhibit type III secretion at concentrations that do not prevent bacterial growth.
Several of the proteins that constitute the Ysc TTS system for Yop secretion have homologs in the secretion system used for assembly of the flagellum18. It is possible that compound that target Yop secretion target other type III secretion mechanisms e.g. flagellum assambly and motiliy. In order to study the possibility for inhibitors of Yop TTS to inhibit TTS flagellum assembly, bacterial motility and Yop transcription in presence of a compound according the invention were investigated. The experiments were carried out in six-well plates with Ca2+ depleted migration agar containing different concentrations of the compound. Motility was found to be inhibited to 10, 40, 60, and 80% at 5, 10, 20, and 50 μM, respectively, of the compound of the invention.
According to the invention there are thus disclosed agents which affect bacterial virulence but do not have a detrimental effect of bacterial growth. The bacterial virulence-affecting agents of the invention are agents which interfere with the type III secretion system in Yersinia.
In particular, according to the invention, there are disclosed agents which decrease bacterial virulence. More particularly the agents of the invention decrease expression of bacterial virulence factors while not substantially affecting bacterial growth.
The agents of the invention capable of decreasing bacterial virulence comprise the structural element R—CO—NH—S-T, wherein R is aromatic or heteroaromatic carbon, S is zero or —N═CH, and T is unsubstituted or substituted aryl including heteroaryl.
According to a first preferred aspect of the invention the agents decreasing bacterial virulence are amides of the general formula I
A-CO—NH—B (I)
wherein
If A is substituted aryl or heteroaryl, it is preferred to be mono- or disubstituted by one or more of halogen, nitro, hydroxy, alkoxy, C1-C6 alkyl, C1-C6 alkenyl, halogen being preferably selected from Cl, Br, I and, independently, alkoxy preferably being acetoxy.
It is preferred for Y to be selected from aryl and heteroaryl substituted with one or several of halogen, C1-C6 alkyl, C1-C6 alkenyl, halogen being preferably being selected from F, Cl, Br.
According to another preferred aspect of the invention the agents decreasing bacterial virulence are amides of the general formula I
A-CO—NH—B (I)
wherein
A is substituted or unsubstituted aryl, heteroaryl, substituted or unsubstituted aryloxy or carbamyl;
B is —X—Y, wherein X is zero, —N═CH— or —CO— and Y is selected from unsubstituted aryl, unsubstituted heteroaryl, mono-, di- and tri-substituted aryl, mono-, di- and tri-substituted heteroaryl, with the proviso that, if X is —N═C—H—, Y is 2-hydroxyaryl.
If A is substituted aryl or heteroaryl, it is preferred to be mono- or disubstituted by one or more of halogen, nitro, hydroxy, alkoxy, C1-C6 alkyl, C1-C6 alkenyl. It is preferred for Y to be selected from aryl and heteroaryl substituted with one or several of halogen, C1-C6 alkyl, C1-C6 alkenyl.
Preferred compounds of the general formula I are shown in
It is also preferred for a compound of the invention to inhibit, at a concentration of 50 μM, the luciferase light signal from pIB29EL by at least 40%, more preferred by at least 60%, most preferred by at least 80%.
Activity and physical data of preferred compounds of the invention are listed in Tables 2A-2C.
The inhibitory agents of the invention can be used in the treatment of infections caused by gram negative bacteria. The treatment comprises administering to a person suffering from such infection an antibacterially effective amount of an agent of the invention. Administration can be in any adequate form, such as, for example per-oral, IV or topical. The compounds of the invention can be incorporated in pharmaceutical compositions for such administration, e.g., tablets and capsules for oral administration, solutions for intravenous and intramuscular administration, and ointments for topical administration.
A person skilled in the art will appreciate that the antibacterial agents of the invention are not antibacterial agents in the classical sense but are agents that modify the virulence of bacteria and thereby exhibit antibacterial effect.
The invention will now be explained in more detail by reference to preferred embodiments illustrated by a number of Figures showing:
FIGS. 1A,B (A) The current model for regulation of Yop and Syc expression involving temperature and Ca2+ dependence via the positive element LcrF and the negative element LcrQ;
(B) A Schematic representation of reporter gene constructs for identification of inhibitors or activators of Yersinia type III secretion;
FIGS. 2A,B Screening readout obtained when performing the assay with bacteria (pIB29EL) alone in absence or presence of Ca2+;
(A) Photo of wells from an assay 96-well plate that contain bacteria in absence or presence of Ca2+;
(B) Quantification of the light signals in absence (black bar) or presence (white bar) of Ca2+;
FIGS. 6A,B Inhibition of Yop secretion by compound 3 Yop secretion from the wild-type strain pIB102EL in presence of various concentrations of 3 investigated with Western analysis.
(A) Analysis of secreted Yops present in the surrounding medium;
(B) Analysis of total intracellular and medium Yop content;
General. A compound collection consisting of 9,400 unique substances (ChemBridge, DiverSet F) was screened for inhibition of the luxAB reporter signal using the non-virulent strain pIB29EL. The compounds were screened in duplicate at a final concentration of 20 μg/mL. Assay interfering compounds, e.g. potential luciferase inhibitors, were identified by addition of hits from the primary screen to assay wells 10-20 min prior to addition of n-decanal. Compounds showing activity in one of the duplicate wells were re-screened to confirm activity or the lack thereof. The complete screening campaign gave about 60 compounds that caused at least 40% inhibition (duplicate average) of the luciferase light signal. For most of these compounds a dose/response relationship was established, and inhibition of bacterial growth was examined. A number of the compounds were found to have an IC50 for inhibition of the luciferase light signal of 50 μM or less. Compounds 3-5 are representative examples of compounds that show a clear selectivity for inhibition of the luciferase light signal over growth inhibition indicating that these substances possibly act on targets directly or indirectly regulating expression of the Yops, but are not required for growth (
Detailed investigations were also carried out for the majority of the active compounds although most emphasis was put on compounds 3-5. All experiments based on luciferase-based readout were carried out in triplicate or quadruplicate in the same manner as in primary screening. As a first step the compounds were assayed with the wild-type control strain, pIB102AL, with luxAB downstream of the promoter for YerA, the chaperone for YopE. As seen in
In order to zoom in on potential targets and to further support a selective mode of action for compounds 3-5 two additional reporter gene strains were employed. Based on the regulatory model in
The investigation was continued by Western analysis of inhibition of actual Yop secretion in presence of compounds 3-5. The wild-type strain pIB102EL was grown at ambient temperature (ca. 21° C.) in presence or absence of different compound concentrations for one hour and then the temperature was raised to 37° C. for induction and secretion of the Yops. After three hours at 37° C. the bacteria were removed by centrifugation, and the protein content in the supernatant, i.e. the surrounding media, was examined by Western analysis using a polyclonal serum active against all secreted proteins. As seen in
Bacterial strains and growth condition. All strains used are Y. psedotuberculosis serotype III (YPIII) and in the following text strains are only labelled with the name of the virulence plasmid. Strains with the luxAB contruct were prepared from either the non-virulent YopH and yadA mutant Y. pseudotuberculosis pIB2928 or the wild type YadA mutant pIB10227 by constructing yopE-luxAB, yer-luxAB, lcrF-luxAB and lcrFΔhlh-luxAB operon fusions essentially as described previous26. The resulting strains pIB29EL (yopE-luxAB), pIB29AL (yerA-luxAB), pIB102EL (yopE-luxAB, wild-type), pIB102AL (yer-luxAB, wild-type), pIB102FL (lcrF-luxAB, wild-type), and pIB1102FΔhlhL (lcrFΔhlh-luxAB, wild-type) strains were struck and grown at room temperature on LB-plates containing chloramphenicol (Sigma) at a final concentration of 50 μg/mL. From plates not older than one week, bacteria for experiments were grown in liquid brain/heart infusion (BHI) broth (Oxoid; Unipath Ltd., Basington, UK) containing 2.5 mM CaCl2 or 20 mM MgCl2 and 5 mM EGTA for Ca2+ depletion.
Compounds, antibiotics and antibodies. The chemical library that consists of 9,400 unique compounds in 96-well plate format was purchased from ChemBridge (Diverset F). The compounds were dissolved in DMSO to give a stock solution of 2 mg/mL. For compounds further characterized in the described experiments additional 5 or 10 mg samples were purchased from ChemBridge. Streptomycin (Sigma), carbenicillin (Sigma), polymyxin B (mixture of polymyxin B1 and B2, Fluka), and nalidixic acid (Sigma) were dissolved in water. Antibodies against the different Yersinia proteins were available from other projects at the department of Molecular Biology, Umeå University.
The synthesis of compound 5 has been reported previously (2-[Benzo-2,1,3-thiadiazole-4-sulfonyl)amino]benzoic acids as synthetic intermediates for antihelmintics. Mikhailitsyn F. S., Drusvyatskaya S. K., Uvarova, N. A, patent no. SU1685935; Search for new antiparasitic agents 3. Synthesis of haloid-containing sulfamidobenzamides with benzo-2,1,3,-dithiazole residue in sulfonamide group and study of their acute toxicity. Mikhailitsyn F. S., Lebedeva M. N., Kozyreva N. P., Lychko N. D. Drusvyatskaya S. K., Uvarova, Med. Parazitol. Parazit. Bolezni. 1991 (2) 36-38). Acylated hydrazones of salicylaldehyde e.g. compound 3 and analogs are prepared from the corresponding acylhydrazides and aldehydes as described previously (Cytotoxicity of salicylaldehyde benzoylhydrazone analogs and their transition metal complexes: quantitative structure-activity relationships. Ainscough E. W, Brodie A. M., Denny W. A., Finlay G. J., Gothe S. A., Ranford J. D., J. Inorg. Biochem. 1999 (77) 125-133). Compound 4 and analogs are prepared according to a known method (Method of producing 2-acetoxy-4′-chloro-3,5-diiodobenzanilide. Mikhailitsyn F. S., Petyrov Y. F., Sapozhnikova L. A., USSR patent no. SU 1226807).
General screening and assay conditions. An overnight culture grown at room temperature in BHI medium containing 20 mM MgCl2 and 5 mM EGTA for Ca2+ depletion was diluted to OD600=0.15-0.25. In parallel the compounds to be tested were dispensed into the wells of a 96-well plate (Polysorp FluoroNunc™ Modules, Nunc) containing 50 μL of medium per well. To each well 50 μL of the bacterial solution was added. For compounds dissolved in DMSO the final DMSO concentration was kept below 2%. The plate was incubated on an orbital shaker at room temperature (ca. 21° C.) for 1 h. The temperature was then shifted to 37° C. and incubation with orbital shaking was continued for 2 h. Subsequently the temperature was shifted back to room temperature and the plate was incubated for 2 h without shaking. Finally 100 μL freshly made n-decanal emulsion (Sigma, 10 μL/100 mL water, emulsified by vigorous shaking) was added to each well and the light emission was measured within 1-3 minutes after addition. Light emission was recorded with a light-sensitive charge-coupled device (CCD) camera, DIANA Chemoluminescense detection module (Raytest, Isotopenmessgeräte, GmbH). The intensity of the light signals was quantified using the computer program TINA (version 2.0). Primary screening of the compound library was carried out in duplicate with a final concentration of 20 μg/mL. Other experiments were carried out in triplicate and quadruplicate with modifications as indicated in the text and figure legends. For antibody experiments 5 μL monoclonal mouse ascites solution was used. Results were typically reproduced in at least three independent experiments.
Growth inhibition experiments. Growth inhibition by compounds identified in the screening process was measured by growing bacteria at 37° C. in the presence of different compound concentrations in 96-well plates containing 100 μL bacterial culture in BHI medium with 2.5 mM CaCl2 per well. The experiments were carried out in a Molecular Device Spectramax 340 plate reader with continuous shaking at 37° C. and periodical determination of absorption at 600 nm. Experiments were carried out in triplicate or quadruplicate, and the results were typically reproduced in at least three independent experiments. Values for percent growth inhibition were calculated from differences in growth rate in presence or absence of compound over a time period with approximately linear growth.
Western analysis of protein secretion. An overnight culture grown at room temperature in BHI medium containing 20 mM MgCl2 and 5 mM EGTA for Ca2+ depletion, was diluted to OD600=0.15-0.25. In parallel the compounds to be tested were dispensed into the wells of a 96-well plate containing 50 μL of medium per well. To each well 50 μL of the bacterial solution was added. The plate was incubated on an orbital shaker at room temperature for 1 h. The temperature was then shifted to 37° C. and incubation with orbital shaking was continued for 3 h. Subsequently the cultures were transferred to micro-centrifuge tubes. After brief centrifugation the supernatants were investigated by standard western analysis (12% SDS-PAGE) using a rabbit polyclonal total ant-Yop serum.
Motility Agar Assay
Motility was measured as the movement of bacterial cells through semisolid motility agar (Luria broth containing 0.25% Bactoagar). Around 107 Y. pseudotuberculosis YPIII pIB102EL bacteria in a 2.5 μl drop were placed at the centre of each well in a 6-well plate (Falcon Multiwell) containing 5 ml motility agar supplemented with 5 mM EGTA, 20 mM MgCl2 and different concentrations of test substance in 0.1 ml DMSO per well. The plates were incubated at 22° C. for 18 h to allow for the bacteria to grow and to move out from the centre of the plate. To induce Yop and LuxAB expression, plates were shifted to 37° C. (at this temperature flagellum production stops) and incubation was continued for 3 h. By placing 5 μl of n-decanal in the lid over each well to expose the bacteria to decanal fumes, light emission from the bacteria was induced. Emitted light was monitored and quantified using a charge-coupled device (CCD) camera. Immediately after measurement of light emission, a picture was taken in visible light to document the bacterial migration. Migration was then quantified directly from the picture as the diameter of the zone covered by bacteria.
aExperiments were carried out with the non-virulent screening strain pIB29EL containing the yopE-luxAB construct.
bConcentration resulting in 50% reduction of luciferase light emission under screening conditions.
373.73
262.93
308.83
272.93
313.23
312.93
320.03
319.73
319.13
318.83
306.13
305.93
1)
1H-NMR, 400Mhz, J in Hz
2)Not deterinined
3)(M+H)+,
| Number | Date | Country | Kind |
|---|---|---|---|
| 0202613 | Sep 2002 | SE | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/SE03/01381 | 9/4/2003 | WO | 00 | 10/27/2005 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2004/022775 | 3/18/2004 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 6136541 | Demers et al. | Oct 2000 | A |
| Number | Date | Country |
|---|---|---|
| 2365426 | Feb 2002 | GB |
| WO 9807745 | Feb 1998 | WO |
| WO9911262 | Mar 1999 | WO |
| WO-0243668 | Jun 2002 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 20060134724 A1 | Jun 2006 | US |
