The invention relates to a method for determining the ischemic level of suspected stroke patients, as well as an appropriate biomarker and its corresponding use.
From the WO 2003/046140 a method is known for the detection of heart disease, in particular of myocardial infarction, in which the presence is determined of at least two antigens in a blood sample. It is an early detectable antigen, namely, Glycogen Phosphorylase BB, and a late-elevated antigen, namely, Troponin-I.
According to WO 2008/064903, specific antibodies are used for the detection of native Glycogen Phosphorylase BB in human samples for the diagnosis of acute coronary syndromes, whereas these antibodies are derived from human Glycogen Phosphorylase enzymes BB.
Glycogen Phosphorylase (GP) is an enzyme that occurs in the form of three different isoenzymes in the body. Glycogen Phosphorylase Isoenzyme BB is one of them; it is found in large concentrations in the heart and brain. Glycogen Phosphorylase is generally a glycogen metabolizing allosteric enzyme. The access number of Glycogen Phosphorylase BB in NCB is DSM ACC 2834 and DSM ACC 2835.
The present invention relates, above all, to the so-called stroke (cerebral apoplexy). For example, atherosclerosis leads to a blockage of the arteries, which in turn can lead to CNS-related symptoms, including loss of language and/or modification of the language and also partial or total paralysis of certain areas of the body, including the facial muscles and/or other areas of the body, or similar symptoms. The therapeutic regimen includes an anticoagulant, and/or anti-platelet medications and thrombolytic agents and is critical for the diagnosis of ischemic stroke and/or a partial or full success of the revascularizations.
The objective of the present invention is to significantly improve the detection of stroke.
The process according to the invention contains the following steps that are used to determine the ischemic level of suspected stroke patients:
In a preferred embodiment, Glycogen Phosphorylase BB shows an epitope as described in the above-mentioned WO 2008/064903. Furthermore, Glycogen Phosphorylase BB is supposed to have a sequence as described in the international patent application.
The present invention also comprises a diagnostic biomarker for detecting ischemic levels of suspected stroke patients, characterized by determining the concentration of Glycogen Phosphorylase BB in the suspects' blood samples, whereas the determination takes place according to the process as described in particular in WO 2008/064903 or EP 1461616.
Another claim is the use for the determination of Glycogen Phosphorylase BB concentration in the blood of suspected stroke patients for determining a stroke.
The stroke patients were older in comparison with non-stroke patients.
Between the stroke patients, clinical variables had no or only a slight influence on the GPBB concentration
3. Commonality and Correlation Analysis
GPPB correlated with P Enkephalin, with glucose and GOT (heart muscle damage or liver cell damage)
No correlation was found to the lipids (CHOL, HDL, LDL, TG), thrombocytes, Ceratinin, CrP
Number | Date | Country | Kind |
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10 2011 051 076.1 | Jun 2011 | DE | national |
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/EP2012/061012 | 6/11/2012 | WO | 00 | 3/17/2014 |