Claims
- 1. A method for depleting viral and prion pathogens in a biological material comprising at least one biological substance, wherein the method comprisesmixing the biological material with an organic solvent to obtain a solvent-containing biological material, contacting the solvent-containing biological material with an ion exchanger to adsorb pathogens on the ion exchanger, wherein at least one biological substance is at least substantially non-interactive with the ion exchanger, and separating the ion exchanger from the biological material, thereby recovering a pathogen-depleted preparation comprising at least one biological substance.
- 2. A method as set forth in claim 1, wherein said ion exchanger is an anion exchanger.
- 3. A method as set forth in claim 2, wherein said anion exchanger is a DEAE-type anion exchanger.
- 4. A method as set forth in claim 3, wherein said DEAE-type anion exchanger is DEAE-Sephadex.
- 5. A method as set forth in claim 1, wherein said organic solvent is used at a concentration of from 5 to 20% (v/v).
- 6. A method as set forth in claim 1, wherein said organic solvent is used at a concentration of from 10 to 15% (v/v).
- 7. A method as set forth in claim 1, wherein said organic solvent is used at a concentration of from 12 to 14% (v/v).
- 8. A method as set forth in claim 1, wherein said organic solvent is selected from the group consisting of monovalent and polyvalent alcohols.
- 9. A method as set forth in claim 1, wherein said organic solvent is ethanol.
- 10. A method as set forth in claim 1, wherein said biological material is incubated with said ion exchanger at a temperature of below 10° C.
- 11. A method as set forth in claim 10, wherein said temperature is below 5° C.
- 12. A method as set forth in claim 10, wherein said temperature ranges between 0 and -10° C.
- 13. A method as set forth in claim 1, wherein said biological material is incubated with said ion exchanger at a pH of between 5.2 and 7.2.
- 14. A method as set forth in claim 13, wherein said pH ranges between 6.0 and 6.4.
- 15. A method as set forth in claim 13, wherein said pH is approximately 6.2.
- 16. A method as set forth in claim 1, wherein said biological material is incubated with said ion exchanger for 1 to 20 hours, wherein the biological material is incubated by a batch approach or a continuous flow approach.
- 17. A method as set forth in claim 16, wherein said incubation is for 4 to 8 hours.
- 18. A method as set forth in claim 9, wherein said ethanol has a concentration of from 5 to 20%.
- 19. A method as set forth in claim 9, wherein said ethanol has a concentration of from 10 to 15%.
- 20. A method as set forth in claim 9, wherein said ethanol has a concentration of from 12 to 14%.
- 21. A method as set forth in claim 1, wherein said biological substance is a blood factor.
- 22. A method as set forth in claim 1, wherein said separating of said ion exchanger is performed by filtration.
- 23. The method according to claim 1, wherein the pathogen is a virus.
- 24. The method according to claim 1, wherein the pathogen is a prion.
Parent Case Info
This application is a continuation of application Ser. No. 09/445,862, filed May 18, 2000, which is a 371 of PCT/AT98/00143, filed Jun. 10, 1998.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5773277 |
Hashimoto et al. |
Jun 1998 |
A |
Foreign Referenced Citations (1)
Number |
Date |
Country |
355000304 |
Jan 1980 |
JP |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/445862 |
|
US |
Child |
09/934330 |
|
US |