Patent Application
20110020832
The present invention relates to a method for detecting a cancer cell caused by HPV, a method for determining whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage, and a primer set and a kit used therefor.
A human papillomavirus (hereinafter referred to as “HPV”) is a virus having circular double-stranded DNA as genome, which induces proliferative lesions. HPV is classified into 100 or more kinds of subtypes. Also, it is known that the subtypes of HPV share common regions, which are nonstructural protein-coding E1 region, E2 region, E4 region, E5 region, E6 region and E7 region, capsid protein-coding L1 region and L2 region, and LCR.
HPV DNA has been detected in lesion sites of uterine cervical cancer and uterine cervical dysplasia, and tissues of oral cancer and pharyngeal cancer. Thus, HPV infection is regarded as one of the risk factors for uterine cervical cancer, oral cancer, and pharyngeal cancer. In most cases, a pattern of HPV infection is a transient infection in which HPV is spontaneously disappeared from a cell after a certain period of time from the establishment of infection. However, in 5 to 10% of HPV infections, there are cases where HPV is not disappeared and the infection turns into a persistent infection causing uterine cervical cancer.
It is to be noted that, in tissue diagnosis of uterine cervix, uterine cervical dysplasia is classified into three stages, namely mild-grade, moderate-grade, and high-grade dysplasia as a preliminary stage of generation of a cancer cell, depending on the degree of appearance of an atypical cell in the epithelium. When high-grade dysplasia is further aggravated, lesions of uterine cervical dysplasia reach a stage at which a cancer cell emerge in the epithelium. Then, uterine cervical dysplasia progresses to “intraepithelial carcinoma”, in which cancer cells are confined to the epithelium, and “microinvasive squamous cell carcinoma” and “invasive squamous cell carcinoma”, in which a cancer cell infiltrates from the epithelium down into subcutaneous tissues.
In most cases, lesions of mild-grade dysplasia or moderate-grade dysplasia are observed without particularly providing any treatment thereto. However, if a precursor lesion of high-grade dysplasia is left untreated, the lesion is highly likely to progress to invasive cancer. Thus, in many cases, treatment such as surgery is provided to a subject diagnosed as having high-grade dysplasia. Accordingly, determining whether or not a lesion in a subject is at a stage of high-grade dysplasia or a more severe stage is important in deciding a treatment method for the subject.
In chromosomal DNA in higher eukaryotes, among bases constituting DNA, the 5-position of cytosine is occasionally methylated. The methylation of DNA in higher eukaryotes functions as an inhibitory mechanism on the expression of genetic information. Recently, a report indicating that the presence or absence of methylation in HPV genomic DNA is strongly associated with onset of cancer has been made.
For example, Patent Document 1 describes that, when E6 region and LCR of HPV contained in a uterine cervical cell of a patient are not methylated, the presence of a uterine cervical cancer cell is more strongly indicated. However, there are cases where unmethylated E6 region and unmethylated LCR are detected in a cell other than a uterine cervical cancer cell. Thus, it is difficult to detect a cancer cell caused by HPV in uterine cervical cells of a patient with high accuracy only by confirming the methylation state of E6 region and LCR.
Also, Non-Patent Document 1 describes that, in uterine cervical cancer, L1 region is strongly methylated, whereas LCR and E6 region are not methylated in HPV-18. In Non-Patent Document 1, a nucleic acid that has been treated with a bisulfite salt, which corresponds to HPV-18 genomic DNA, is sequenced, and the methylation state thereof is confirmed. However, confirming methylation of DNA by sequencing is time-consuming and involves cumbersome operations. Thus, it is difficult to detect a cancer cell caused by HPV in a sample collected from a subject in a simple manner.
[Non-Patent Document 1] Tolga Turan et al., Virology 349 (2006) pp. 175-183
[Patent Document 1] National Publication of International Patent Application No. 2006-522607
An object of the present invention is to provide a method for detecting a cancer cell caused by HPV easily with high accuracy. Another object of the present invention is to provide a method for determining whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage easily with high accuracy. Still another object of the present invention is to provide a primer set capable of detecting a cancer cell caused by HPV and determining whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage easily with high accuracy. Yet still another object of the present invention is to provide a kit capable of diagnosing cancer caused by HPV easily with high accuracy. Yet still another object of the present invention is to provide a kit capable of diagnosing the stage of dysplasia.
That is, the present invention relates to the followings:
(1) A method for, detecting a cancer cell caused by HPV, comprising the steps of:
(2) the method according to the above (1), wherein the step (A) comprises the steps of:
(3) the method according to the above (2), wherein the step (A) further comprises the step of:
wherein, in the step (b), a product obtained in the step (a1) is subjected to centrifugation, thereby precipitating an insoluble matter, to give a supernatant;
(4) a method for determining whether or not a tissue is a tissue at a stage of high-grade dysplasia or a more severe stage, comprising the steps of:
(5) the method according to the above (4), wherein the tissue is a uterine cervical tissue;
(6) a primer set comprising:
a first primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV; and
a second primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR or E6 region of HPV,
wherein the primer set is used to amplify a nucleic acid consisting of a continuous nucleotide sequence ranged from the site with which the first primer is hybridized to the site with which the second primer is hybridized by nucleic amplification reaction, among nucleic acids each consisting of nucleotide sequence of HPV genomic DNA in which unmethylated cytosine is converted into another base.
(7) the primer set according to the above (6), wherein the first primer is a primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region of HPV.
(8) the primer set according to the above (6), wherein the second primer is a primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR of HPV.
(9) the primer set according to the above (6), wherein the another base is uracil or thymine;
(10) the primer set according to the above (6), wherein the first primer and the second primer are primers used for amplifying a nucleic acid by a polymerase chain reaction method, a strand displacement amplification method, a ligase chain reaction method, on a transcriptional amplification method;
(11) a diagnostic kit for a cancer caused by HPV comprising;
the primer set of the above (6), and
an unmethylated cytosine-conversion agent for converting unmethylated cytosine in a nucleic acid into another base;
(12) the diagnostic kit according to the above (11), wherein the unmethylated cytosine-conversion agent is a bisulfite salt;
(13) the diagnostic kit according to the above (11), wherein the cancer caused by HPV is uterine cervical cancer, oral cancer, or pharyngeal cancer; as well as,
(14) a diagnostic kit for a stage of dysplasia comprising;
the primer set of the above (6), and
an unmethylated cytosine-conversion agent for converting unmethylated cytosine in a nucleic acid into another base.
The primer set of the present invention contains a first primer which hybridizes with, in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV (also referred to as “first nucleotide sequence”), a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base (also referred to as “second nucleotide sequence”), and a second primer which hybridizes with, in a nucleotide sequence having a CpG site in LCR or E6 region of HPV (also referred to as “third nucleotide sequence”), a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base (also referred to as “fourth nucleotide sequence”). The primer set is used to amplify, among nucleic acids including nucleotide sequences obtained by converting unmethylated cytosine into another base, which correspond to HPV genomic DNA, a nucleic acid consisting of a continuous nucleotide sequence from a site with which the first primer hybridizes to a site with which the second primer hybridizes by nucleic amplification reaction. The second nucleotide sequence is a nucleotide sequence of a nucleic acid resulting from conversion of a nucleic acid including the first nucleotide sequence, and the second nucleotide sequence corresponds to the first nucleotide sequence except that cytosine present in a site other than a CpG site has been converted into another base. Also, the fourth nucleotide sequence is a nucleotide sequence of a nucleic acid resulting from conversion of a nucleic acid including the third nucleotide sequence, and it corresponds to the third nucleotide sequence except that all of cytosine has been converted into another base.
One of the significant features of the primer set of the present invention is that the primer set contains the first primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV, and the second primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR or E6 region of HPV.
In HPV genomic DNA contained in a cancer cell caused by HPV, cytosine in a CpG site in L2 region or L1 region is methylated, and cytosine in a CpG site in LCR or E6 region is unmethylated. Thus, using the primer set of the present invention for nucleic acid amplification reaction, a nucleic acid consisting of a nucleotide sequence in which unmethylated cytosine is converted into another base, which corresponds to genomic DNA of canceration-causing HPV, can be specifically amplified. As described above, according to the primer set of the present invention, a cancer cell caused by HPV can be easily detected with high accuracy. Therefore, according to the primer set of the present invention, diagnosis of uterine cervical cancer, oral cancer, and pharyngeal cancer can be easily carried out with high accuracy.
Further, in HPV genomic DNA present in lesions at a stage of high-grade dysplasia or a more severe stage, cytosine in a CpG site in L2 region or L1 region is methylated, and cytosine in a CpG site in LCR or E6 region is unmethylated. Thus, using the primer set of the present invention for nucleic acid amplification reaction, a nucleic acid consisting of a nucleotide sequence in which unmethylated cytosine is converted into another base, which corresponds to HPV genomic DNA present in lesions at a stage of high-grade dysplasia or a more severe stage, can be specifically amplified. Therefore, according to the primer set of the present invention, stage of dysplasia can be easily diagnosed with high accuracy. Also, according to the primer set of the present invention, whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage can be easily determined with high accuracy.
As used herein, a cancer cell caused by HPV refers to a cell that has become cancerous or is at a high risk of becoming cancerous due to HPV infection. More specifically, a cancer cell caused by HPV refers to an HPV-infected cell causing onset of uterine cervical cancer, oral cancer or pharyngeal cancer, a uterine cervical cancer cell, an oral cancer cell, or a pharyngeal cancer cell.
As used herein, “a stage of high-grade dysplasia or a more severe stage” refers to the stage of cancer that is classified into “high-grade dysplasia” or a more severe stage, namely “intraepithelial carcinoma”, “microinvasive squamous cell carcinoma”, or “invasive squamous cell carcinoma”, according to the classification based on “The General Rules for Clinical and Pathological Management of Uterine Cervical Cancer 1997” edited by Japan Society of Obstetrics and Gynecology. When lesions in tissues are determined to be at the above stages, in most cases a subject requires treatment such as surgery. Thus, the determination of whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage is crucial in the clinical practice. On the other hand, lesions that are milder than “high-grade dysplasia” are classified into lesions at a stage of “no abnormality in the epithelium”, “mild-grade dysplasia”, or “moderate-grade dysplasia”. When lesions are at the above stages, in most cases a subject is observed without receiving any particular treatment.
As used herein, “an abnormal cell” refers to “an atypical cell” and “a cancer cell”. Herein, “an atypical cell” refers to a cell that is not a cancer cell but has abnormal nuclei such as enlarged nuclei, increased chromatin, and irregularly-shaped nuclei.
As described above, a cancer cell caused by HPV has HPV genomic DNA in which cytosine in a CpG site in L2 region or L1 region is methylated, and cytosine in a CpG site in LCR or E6 region is unmethylated. When HPV genomic DNA contained in the cancer cell caused by HPV is treated with an unmethylated cytosine-conversion agent that converts unmethylated cytosine into another base, cytosine in a CpG site in L2 region or L1 region is not converted into another base, whereas cytosine in a CpG site in LCR or E6 region is converted into another base. Accordingly, the use of the primer set of the present invention enables amplification of, among HPV genomic DNA that has been treated with an unmethylated cytosine-conversion agent, only a nucleic acid in which a CpG site in L2 region or L1 region remains to be a nucleotide sequence of a dinucleotide including cytosine and guanine, while cytosine in a CpG site in LCR or E6 region is converted into another base by nucleic acid amplification reaction.
Further, in the primer set of the present invention, the first primer hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV. Also, the second primer hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR or E6 region of HPV. Thus, the primer set of the present invention enables amplification of a nucleic acid that has been treated with an unmethylated cytosine-conversion agent, which corresponds to DNA of a continuous region containing at least a part of L2 region or L1 region and at least a part of LCR or E6 region in HPV genomic DNA, which causes HPV-infected cells to become cancerous, by single nucleic acid amplification reaction.
HPV is a virus containing approximately 8 kb of circular DNA. The HPV includes, for example, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV58, HPV59, HPV68, HPV73, HPV82 and the like.
As shown in
Generally, when HPV infects and invades a cell, cytosine in a certain CpG site among CpG sites in HPV genomic DNA are methylated by DNA methylation mechanism of a living body. Especially, as shown in
Table 1 shows the methylation state of CpG sites in L1 region and L2 region as well as LCR and E6 region in HPV genomic DNA contained in an HPV-infected cell. Herein, among the HPV-infected cells shown in Table 1, HPV-infected cell 2 indicates a cancer cell caused by HPV. It is to be noted that, in Table 1, “◯” indicates that cytosine in a CpG site is methylated, whereas “×” indicates that cytosine in a CpG site is unmethylated.
As described above, methylation of DNA is detected in CpG sites in L1 region and L2 region in HPV in a uterine cervical cancer cell. However, as shown in Table 1, there are cases where methylation of DNA in L1 region or L2 region are observed also in an HPV-infected cell that is not a cancer cell caused by HPV. Thus, when only methylation in L1 region or L2 region is used as an index for detection of a cancer cell caused by HPV, there are cases where it is difficult to distinguish a cancer cell caused by HPV from other HPV-infected cells. That is, when only methylation in L1 region or L2 region is used as an index for detection of a cancer cell caused by HPV, in some cases not only HPV-infected cell 2 shown in Table 1, which indicates a cancer cell caused by HPV, but also HPV-infected cell 1 may be detected.
Further, CpG sites in LCR and E6 region in HPV in a uterine cervical cancer cell are unmethylated. However, there are cases where CpG sites in LCR or E6 region are unmethylated also in an HPV-infected cell and the like that is not a cancer cell caused by HPV. Thus, when only the state of unmethylation in LCR or E6 region is used as an index for detection of a cancer cell caused by HPV, there are cases where it is difficult to distinguish a cancer cell caused by HPV from other HPV-infected cells. That is, when only the state of unmethylation in LCR or E6 region is used as an index for detection of a cancer cell caused by HPV, in some cases not only HPV-infected cell 2 shown in Table 1, which indicates a cancer cell caused by HPV, but also HPV-infected cell 4 may be detected.
However, the primer set of the present invention enables specific amplification of a nucleic acid that has been treated with an unmethylated cytosine-conversion agent, which corresponds to HPV genomic DNA contained in HPV-infected cell 2 shown in Table 1, by nucleic acid amplification reaction. Thus, with use of the primer set of the present invention, an excellent effect is exerted that HPV-infected cell 2, which indicates a cancer cell caused by HPV, can be specifically detected from among HPV-infected cells 1 to 4 shown in Table 1.
On the other hand, also in HPV contained in a tissue at a stage of high-grade dysplasia or a more severe stage, cytosine in CpG sites in L1 region and L2 region is methylated, whereas cytosine in CpG sites in LCR and E6 region is unmethylated. Accordingly, with use of the primer set of the present invention, an excellent effect is exerted that HPV DNA in a tissue at a stage of high-grade dysplasia or a more severe stage can be specifically detected.
As used herein, “a primer hybridizes” means that a primer hybridizes with a nucleic acid under stringent conditions. The state where a primer has hybridized with a nucleic acid is a state where the primer is annealed to a complementary sequence in a nucleic acid in a state suitable for carrying out nucleic acid amplification reaction. Herein, the stringent conditions refer to the conditions that are generally used by those skilled in the art when hybridization of polynucleotide is carried out. The stringent conditions are not particularly limited, as long as it is a condition under which the primer set of the present invention can hybridize with a nucleic acid having a nucleotide sequence of interest in a nucleic acid that has been treated with an unmethylated cytosine-conversion agent, which corresponds to HPV genomic DNA. The stringency in hybridization is known as a function of temperature, salt concentration, length of primer, GC content in a nucleotide sequence of primer, and concentration of chaotropic agent in a hybridization buffer. As the stringent conditions, for example, the conditions set forth in Sambrook, J. et al. (1998) Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York and the like can be used.
It is to be noted that “another base” includes uracil and thymine. For example, when a bisulfite salt is used as an unmethylated cytosine-conversion agent, unmethylated cytosine is converted into uracil. Also, uracil thus converted will be converted into thymine by nucleic acid amplification reaction using the primer set of the present invention.
The first primer and the second primer are primers used to amplify a nucleic acid by nucleic acid amplification. Specifically, the first primer and the second primer are preferably primers each used to amplify a nucleic acid by a polymerase chain reaction method, a strand displacement amplification method, a ligase chain reaction method, or a transcriptional amplification method. Thus, by carrying out nucleic acid amplification reaction with the primer set of the present invention, using as a template DNA which has been extracted from a cell of a subject and has been treated with an unmethylated cytosine-conversion agent, a subject having a cancer cell caused by HPV and a tissue at a stage of high-grade dysplasia or a more severe stage can be easily confirmed based on the results of the amplification reaction.
The polymerase chain reaction method can be carried out in accordance with a conventional technique. The strand displacement amplification method includes, for example, LAMP method, ICAN (trademark) method, SMAP method and the like. The transcriptional amplification method includes, for example, TAS method and the like.
The primer set of the present invention can be produced by the known method, depending on the kind of nucleic acid amplification method to be employed. More specifically, regarding each of a nucleotide sequence of L1 region or L2 region and a nucleotide sequence of LCR or E6 region, a nucleotide sequence after treatment with an unmethylated cytosine-conversion agent is predicted. Subsequently, the primer set of the present invention can be produced by designing a nucleotide sequence of each primer in the primer set of the present invention with a commercially available primer design software and the like based on the nucleotide sequence thus predicted, and synthesizing each primer. The software used to design each primer of the primer set used for real-time PCR, which is polymerase chain reaction method, includes, for example, GENETYX, primer 3 and the like. Also, the software used to design each primer of the primer set used for LAMP method, which is strand displacement amplification, includes, for example, Primer Explorer and the like.
Since a cancer cell caused by HPV can be detected as described above according to the primer set of the present invention, cancer caused by HPV can be diagnosed. Accordingly, the present invention encompasses a diagnostic kit for cancer caused by HPV (hereinafter, also referred to as “diagnostic kit 1”).
The diagnostic kit 1 of the present invention is a kit containing the primer set and an unmethylated cytosine-conversion agent that converts unmethylated cytosine, in a nucleic acid into another base.
The unmethylated cytosine-conversion agent may be an agent, for converting unmethylated cytosine in a nucleic acid into another base, and examples thereof include a bisulfite salt and the like. The bisulfite salt includes, for example, sodium bisulfite and the like.
In the diagnostic kit 1 of the present invention, the primer set is dissolved in a solvent such as buffer suitable for stably maintaining nucleic acid, and is provided in a sealed container and the like suitable for stably maintaining nucleic acid. Also, the unmethylated cytosine-conversion agent is dissolved in a solvent suitable for dissolving the unmethylated cytosine-conversion agent, and is provided in a sealed, appropriate container and the like.
The cancer caused by HPV to which the diagnostic kit 1 of the present invention is applicable includes, for example, uterine cervical cancer, oral cancer, pharyngeal cancer and the like.
According to the primer set of the present invention, a cancer cell caused by HPV can be detected as described above. The present invention also encompasses a method for detecting a cancer cell caused by HPV.
The method for detecting a cancer cell of the present invention includes the steps of:
In the method for detecting a cancer cell of the present invention, a sample containing DNA is prepared from a cell of a subject (step (A)).
In the step (A), preparation of a sample containing DNA from a cell of a subject can be carried out by the known method and the like. For example, preparation of the sample containing DNA can be carried out by the steps of:
Further, the step (A) may further include a step (al) of subjecting the mixture obtained in the step (a) to a physical treatment, thereby isolating DNA from the cell, between the step (a) and the step (b). In the above case, in the step (b), a product obtained in the step (a1) is subjected to centrifugation, thereby precipitating an insoluble matter to give a supernatant.
The surfactant includes, for example, sodium dodecyl sulfate (SDS), sodium tetradecyl sulfate, sodium dodecyl sulfonate, sodium tetradecyl sulfonate, sodium cholate (CHO), sodium deoxycholate (DOC), sodium taurocholate, sodium taurodeoxycholate and the like. Sodium dodecyl sulfate (SDS) is particularly preferable. Also, as a method of physical treatment, the known method for disrupting physically a cell can be used. Examples thereof include a method for disrupting a cell with a homogenizer and a method for disrupting a cell by shaking the cell with a mixer. A commercially available kit for extraction of DNA can be used in the preparation of a sample containing DNA.
DNA extracted as above can be dissolved in water or buffer, and provided as a DNA solution. Water and buffer for dissolving DNA are preferably those capable of stably maintaining dissolved DNA. The water and buffer for dissolving DNA includes, for example, PCR-grade water free from nucleases, TE solution (10 mM Tris-HCl buffer (pH 8.0), 1 mM EDTA) and the like.
The cell of a subject may be a target cell for HPV infection or a target cell for HPV latent infection. The cell of a subject is not particularly limited. The cell includes, for example, a mucosal cell and a skin cell. The mucosa includes, for example, the mucosa of the inner wall of a hollow organ such as genitourinary apparatus, digestive organ, respiratory apparatus and the like. More specific examples of the mucosa include uterine cervical mucosa, oral and pharyngeal mucosa and the like. Among the cell of a subject, a uterine cervical cell and an oral and pharyngeal cell are preferable. According to the detection method of the present invention, by using a uterine cervical cell or a oral and pharyngeal cell as the cell of a subject; a cancer cell caused by HPV causing onset of uterine cervical cancer, oral cancer, or pharyngeal cancer can be detected. The uterine cervical cell is a cell collectable from the uterine cervix (for example, uterine cervical mucosa and the like) of a subject. Also, the oral and pharyngeal cell is a cell collectable from the oral cavity and pharynx (for example, oral and pharyngeal mucosa) of a subject.
Subsequently, unmethylated cytosine in DNA contained in the sample obtained in the step (A) is converted into another base (step (B)).
Conversion of unmethylated cytosine into another base can be carried out by using the unmethylated cytosine-conversion agent. When a bisulfite salt is used as the unmethylated cytosine-conversion agent, methylated cytosine is not converted, whereas unmethylated cytosine is converted into uracil as described above. The bisulfite salt includes, for example, sodium bisulfite and the like. When sodium bisulfite, which is a bisulfite salt, is used as the unmethylated cytosine-conversion agent, conversion of unmethylated cytosine into another base can be carried out by adding 10 M sodium bisulfite solution to a sample containing DNA, and incubating the mixture thus obtained under appropriate temperature conditions.
Subsequently, using the conversion sample obtained in the step (B), the first primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV, and the second primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR or E6 region of HPV, nucleic acid amplification reaction is carried out to amplify a nucleic acid including a continuous nucleotide sequence from a site with which the first primer hybridizes to a site with which the second primer hybridizes (step (C)). Because nucleic acid amplification reaction is carried out using the conversion sample, the first primer and the second primer in the step (C) in the detection method of the present invention, a nucleic acid in which unmethylated cytosine is converted into another base, which corresponds to genomic DNA of cancerivation-causing HPV, can be specifically amplified. Therefore, according to the detection method of the present invention, a cancer cell caused by HPV can be detected with high accuracy.
The nucleic acid amplification reaction is carried out by the polymerase chain reaction method, the strand displacement amplification method, the ligase chain reaction method, or the transcriptional amplification method as described above. Among them, the polymerase chain reaction method is preferable.
In the nucleic acid amplification reaction using the primer set, an amplification product will be obtained when HPV genomic DNA in which cytosine in a CpG site in L2 region or L1 region is methylated, and cytosine in a CpG site in LCR or E6 region is unmethylated is present in the sample containing DNA that has been treated with the above-described unmethylated cytosine-conversion agent. That is, an amplification product will be obtained when HPV genomic DNA shown as HPV-infected cell 2 in Table 1 is present in the sample containing DNA of the cell of a subject. On the other hand, an amplification product will not be obtained when only HPV genomic DNA shown as an HPV-infected cell 1, 3 and 4 as shown in Table 1 are present in the sample containing DNA of the cell of a subject.
Subsequently, a cancer cell caused by HPV is detected based on the results of the nucleic acid amplification reaction in the step (C) (step (D)). The subject is determined to have a cancer cell caused by HPV when an amplification product is obtained by nucleic acid amplification reaction. That is, the presence of the amplification product could be an index indicating that the subject has persistent HPV infection. On the other hand, the subject is determined not to have a cancer cell caused by HPV when an amplification product is not obtained by nucleic acid amplification reaction. In this case, the absence of the amplification product could be an index indicating that the subject is not infected with HPV or, even if the subject is infected with HPV, the subject has a transient infection. As shown above, in the detection method of the present invention, the presence of the amplification product can be easily detected by confirming a cancer cell caused by HPV.
Whether or not an amplification product has been obtained by nucleic acid amplification reaction can be confirmed by the known method. The method includes, for example, an agarose gel electrophoresis method, a method including hybridizing a labeling probe to an amplification product to detect the resultant, a method for detecting fluorescence using an intercalator capable of binding to double-stranded DNA (for example, SYBRGreen and the like), and a method for detecting turbidity caused by a byproduct generated by nucleic acid amplification.
Also, the primer set of the present invention enables determination of whether or not a tissue obtained by a subject is at a stage of high-grade dysplasia or a more severe stage, and also enables diagnosis of the stage of dysplasia. Accordingly, the present invention also encompasses a diagnostic kit for a stage of dysplasia (hereinafter, also referred to as “diagnostic kit 2”).
The diagnostic kit 2 of the present invention is a kit containing the primer set and the unmethylated cytosine-conversion agent. In the diagnostic kit 2 of the present invention, the primer set and the unmethylated cytosine-conversion agent are provided in a form similar to the diagnostic kit 1.
The tissue of a subject to which the diagnostic kit 2 of the present invention is applicable may be a tissue containing a target cell for HPV infection or a tissue containing a target cell for HPV latent infection. The tissue includes, for example, a tissue collected from uterine cervix, or oral cavity and pharynx. It is particularly preferable to apply the diagnostic kit 2 to diagnose a tissue collected from the uterine cervix.
Further, as described above, according to the primer set of the present invention, whether or not a tissue of a subject is at a stage of high-grade dysplasia or a more severe stage can be determined. The present invention also encompasses a method for determining whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage.
The determination method of the present invention includes the steps of:
In the determination method of the present invention, a sample containing DNA is prepared from a tissue of a subject (step (A)).
In the step (A) of the detection method, preparation of a sample containing DNA from a tissue of a subject may be carried by the known method and the like. Also, a sample containing DNA can be prepared from a tissue of a subject by the same operations as in the preparation of a sample containing DNA from a cell of a subject in the step (A) of the method for detecting a cancer cell caused by HPV.
The tissue of a subject may be a tissue containing a target cell for HPV infection or a target cell for HPV latent infection. Specific examples of the tissue of a subject include a tissue collected from the uterine cervix, or oral cavity and pharynx of a subject.
The step (B) and step (C) in the determination method of the present invention can be carried out by the same operations as in the step (B) and step (C) in the method for detecting a cancer cell caused by HPV. Because nucleic acid amplification reaction is carried out using the conversion sample obtained in the step (B), the first primer, and the second primer in the step (C) in the determination method of the present invention, a nucleic acid in which unmethylated cytosine is converted into another base, which corresponds to HPV genomic DNA present in a lesion at a stage of high-grade dysplasia or a more severe stage, can be specifically amplified. Therefore, according to the determination method of the present invention, whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage can be determined with high accuracy.
Subsequently, in the determination method of the present invention, whether or not the tissue is at a stage of high-grade dysplasia or a more severe stage is determined based on the results of the nucleic acid amplification reaction in the nucleic acid amplification step (step (D)). When an amplification product is obtained by the nucleic acid amplification reaction, the tissue of a subject is determined to be at a stage of high-grade dysplasia or a more severe stage. On the contrary, when an amplification product is not obtained by the nucleic acid amplification reaction, the tissue of a subject is determined not to be at a stage of high-grade dysplasia or a more severe stage. As described above, in the determination method of the present invention, whether or not a tissue is at a stage of high-grade dysplasia or a more severe stage is easily determined by confirming the presence or absence of an amplification product, using the presence or absence of the amplification product as an index.
Hereinafter, the present invention will be described in detail based on Examples, but the present invention is not limited thereto.
To 1 μg of genomic DNA of a SiHa cell, which is a cell line derived from uterine cervical cancer having HPV16 genome integrated into its chromosome, 300 μL of 0.3 M sodium hydroxide solution was added, followed by incubation at 37° C. for 10 minutes. Subsequently, 300 μL of 10 M bisulfite salt solution (10 M sodium bisulfite solution) was added to the resulting product, followed by incubation at 80° C. for 40 minutes to carry out bisulfite salt treatment of the genomic DNA. DNA contained in the resulting solution after the bisulfite salt treatment was purified by a DNA purification kit (manufactured by QIAGEN under the trade name of Qiaquick PCR purification kit). To DNA thus purified, sodium hydroxide was added so as to have a final concentration of 0.3 M, followed by incubation at room temperature for 5 minutes. Thereafter, the product thus obtained was purified by a spin column for nucleic acid purification (manufactured by GE Healthcare under the trade name of MicroSpin S-300 HR Columns), to give an analytical sample.
To 2 μL of the analytical sample, 2.5 μL of reagent (×10 buffer) contained in a PCR reagent (manufactured by TAKARA BIO INC. under the trade name of TaKaRa EX Taq (trademark) Hot Start Version), 0.125 μL of DNA polymerase (trade name: TaKaRa Ex Taq HS (5 U/μL)), 2 μL of 2.5 mM dNTP mixture, 1 μL of an aqueous solution of forward primer (10 mM), 1 μL of an aqueous solution of reverse primer (10 mM), and 16.38 μL of water were added to prepare a PCR reaction liquid.
A primer set consisting of the forward primer and the reverse primer and PCR thermal profile used are shown in Table 2.
In Table 2, PCR thermal profile (1) represents conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 40 cycles of reactions, each cycle being 95° C. for 30 seconds, 60° C. for 30 seconds, and 72° C. for 40 seconds. Also, in Table 2, PCR thermal profile (2) represents conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 40 cycles of reactions, each of cycle being 95° C. for 30 seconds, 53° C. for 15 seconds and 72° C. for 30 seconds.
Using the PCR reaction liquid, PCR was carried out under the PCR conditions suitable for the kind of primer set.
The amplification product after PCR was incorporated into vectors included in a TA cloning kit (manufactured by Invitrogen Corporation under the trade name of TA cloning kit). E. coli (TOP 10) was transformed with the construct thus obtained. E. coli thus transformed was cultured at 37° C. overnight on LB agar medium (composition: 1% (w/v) tryptone, 0.5% (w/v) yeast extract, 1% (w/v) sodium chloride, and 1.5% (w/v) agar). Colonies of E. coli thus obtained were inoculated in LB liquid medium and cultured at 37° C. overnight. Then, a plasmid was purified from E. coli thus obtained using a plasmid extraction kit (manufactured by Sigma-Aldrich Corporation under the trade name of GenElute Plasmid Miniprep Kit). The amplification product that had been incorporated into the plasmids thus obtained was determined by BigDye terminator Cycle Sequencing, using a genetic analysis system (manufactured by Applied Biosystems Inc. under the trade name of ABI Prism 3100).
When cytosine in a CpG site is methylated (methylated CpG site), the CpG site appears as “CG” in the nucleotide sequence thus determined. On the other hand, when cytosine in a CpG site is unmethylated, (unmethylated CpG site), the CpG site appears as “TG” in the nucleotide sequence thus determined. Then, based on the nucleotide sequence of the amplification product thus determined, localization of a methylated CpG site and an unmethylated CpG site in HPV16 genomic DNA was analyzed. The results are shown in
From the results shown in
Among the uterine cervical tissues confirmed to be infected with HPV16, a normal tissue in which dysplasia had not pathologically developed and a cancer tissue were each thinly sliced into 20 μm-thick sections to prepare a normal tissue sample and a cancer tissue sample. To each of tissue samples thus obtained, 500 μL of a solution containing 1% (w/v) SDS and 0.1 M sodium hydroxide were added. Each of the mixtures thus obtained were incubated at 100° C. for 20 minutes. Subsequently, the mixtures after incubation were centrifuged at 4° C. to collect each of supernatants.
To each of the supernatants thus obtained, 500 μL of the bisulfite salt solution was added. Subsequently, the mixtures thus obtained were incubated at 80° C. for 40 minutes to carry out a bisulfite salt treatment. Nucleic acids contained in the solutions after the bisulfite salt treatment were purified by a nucleic acid purification kit (manufactured by QIAGEN under the trade name of QIAquick PCR purification kit). To the nucleic acids thus obtained, sodium hydroxide was added so as to have a final concentration of 0.3 M. Subsequently, the mixtures thus obtained were incubated at room temperature for 5 minutes. Thereafter, the products thus obtained were purified by a spin column for nucleic acid purification (manufactured by GE Healthcare under the trade name of MicroSpin S-300 HR Columns), whereby analytical samples were obtained.
To 4 μL of the analytical samples, 2 μL of reagent (trade name: ×10 buffer) contained in a PCR reagent (manufactured by Roche Diagnostics under the trade name of FastStart Taq DNA polymerase), 0.16 μL of DNA polymerase (trade name: FastStart Taq DNA polymerase (5 U/μL)), 1.6 μL of 2.5 mM dNTP mixture, 0.8 μL of an aqueous solution of forward primer (10 μM), 0.8 μL of an aqueous solution of reverse primer (10 μM), and 10.64 μL of water were added to prepare a PCR reaction liquid.
A primer set consisting of the forward primer and the reverse primer used and PCR thermal profiles are shown in Table 3.
In Table 3, the primer set of Example 1 consists of 16L1/LCR-F, which is the forward primer, and 16L1/LCR-R, which is the reverse primer. The 16L1/LCR-F hybridizes with a site corresponding to L1 region containing a given methylated CpG site in a nucleic acid that has been subjected to the bisulfite salt treatment, which corresponds to HPV16 genomic DNA. The 16L1/LCR-R hybridizes with a site corresponding to LCR containing a given unmethylated CpG site in a nucleic acid that has been subjected to the bisulfite salt treatment, which corresponds to HPV16 genomic DNA. That is, when a nucleic acid obtained by subjecting HPV16 genomic DNA in which cytosine in a CpG site in L1 region is methylated and cytosine in a CpG site in LCR is unmethylated to the bisulfite salt treatment is contained in an analytical sample, an amplification product will be generated by carrying out PCR using the primer set of Example 1.
In Table 3, the primer set of Comparative Example 1 consists of 16L1Me1-F, which is the forward primer, and 16L1Me1-R, which is the reverse primer. The 16L1Me1-F and 16L1Me1-R each hybridizes with sites corresponding to the L1 region containing a given methylated CpG site in a nucleic acid that has been subjected to the bisulfite salt treatment, which corresponds to HPV16 genomic DNA. That is, when a nucleic acid obtained by subjecting HPV16 genomic DNA in which cytosine in a CpG site in L1 region is methylated to the bisulfite salt treatment is contained in an analytical sample, an amplification product is generated by carrying out PCR using the primer set of Comparative Example 1.
In Table 3, the primer set of Comparative Example 2 consists of 16LCRUnMe1-F, which is the forward primer, and 16LCRUnMe1-R, which is the reverse primer. The aforementioned 16LCRUnMe1-F and 16LCRUnMe1-R each hybridizes with sites corresponding to the LCR containing a certain unmethylated CpG site in a nucleic acid that has been subjected to the bisulfite salt treatment, which corresponds to HPV16 genomic DNA. That is, when a nucleic acid obtained by subjecting HPV16 genomic DNA in which cytosine in a CpG site in LCR is unmethylated to the bisulfite salt treatment is contained in an analytical sample, an amplification product will be generated by carrying out PCR using the primer set of Comparative Example 2.
Also, in Table 3, PCR thermal profile (3) represents conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 45 cycles of reactions, each cycle being 95° C. for 30 seconds, 60° C. for 15 seconds and 72° C. for 30 seconds. PCR thermal profile (4) represents conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 45 cycles of reactions, each cycle being 95° C. for 30 seconds, 63° C. for 15 seconds and 72° C. for 30 seconds. PCR thermal profile (5) represents conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 45 cycles of reactions, each cycle being 95° C. for 30 seconds, 56° C. for 15 seconds, and 72° C. for 30 seconds.
PCR was carried out using the PCR reaction liquid under PCR conditions suitable for the kind of primer set, thereby confirming the presence or absence of an amplification product. The results are shown in
As apparent from the results shown in panel (a) in
Further, as apparent from the results shown in panel (c) in
In contrast, as apparent from the results shown in panel (b) in
From the above results, it was suggested that a cancer cell caused by HPV can be detected by using the primer set consisting of the first primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region of HPV and the second primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR of HPV.
By similar operations to Experimental Example 1, using C4-1 cell, which is an uterine cervical cancer-derived cell line having the HPV18 genome integrated into its chromosome, a methylated CpG site and an unmethylated CpG site in the HPV18 genome were analyzed. Specifically, except that genomic DNA of the C4-1 cell was used in place of genomic DNA of the SiHa cell and PCR reaction was carried out using the primer set and PCR thermal profile (6) shown in Table 4 in place of the primer set and PCR thermal profiles shown in. Table 2, similar operations to Experimental Example 1 were carried out to determine the nucleotide sequence of an amplification product.
In Table 4, PCR thermal profile (6) represents conditions for carrying out 40 cycles of reactions after keeping the sample at 95° C. for 4.5 minutes, where one cycle consists of 95° C. for 30 seconds, 54° C. for 30 seconds, and 72° C. for 40 seconds.
Then, based on the nucleotide sequence of the amplification product thus determined, localization of a methylated CpG site and an unmethylated CpG site in HPV18 genomic DNA was analyzed. The results are shown in
From the results shown in
Fourteen kinds of uterine cervical tissues each of which was surgically resected from a subject and confirmed to have been infected with HPV16 were provided as paraffin blocks (hereinafter, referred to as “surgically-resected sample”). Also, seven kinds of uterine cervical tissues each of which was collected by scratching the uterine cervix of a subject under colposcopic observation and confirmed to have been infected with HPV16 were provided as paraffin blocks (hereinafter, referred to as “biopsy sample”). Each of the paraffin block was thinly sliced into 10 μm-thick sections to give tissue samples.
The surgically-resected sample and the biopsy sample both include tissues that have been diagnosed to be at a stage of dysplasia or a more severe stage by conventional tissue diagnosis. Among the fourteen kinds of surgically-resected samples, one of them is a sample of mild-grade dysplasia (CIN1), four kinds are samples of moderate-grade dysplasia (CIN2), four kinds are samples of high-grade dysplasia (CIN3), four kinds are samples of cancer (SCC), and another one is a normal sample. Also, among the biopsy samples, two of them are samples of mild-grade dysplasia (CIN1), another two are samples of high-grade dysplasia (CIN3), and the other three are samples of cancer (SCC).
To a 1.5 mL tube, three sections of a tissue sample obtained from the same paraffin block and 1 mL of xylene were added. The mixture thus obtained was centrifuged at 12000 rpm for 10 minutes. After centrifugation, the resulting supernatant was removed and 1 mL of 100% by volume of ethanol was added to the resulting pellet to make a suspension. The suspension thus obtained was centrifuged at 12000 rpm for 10 minutes. After centrifugation, the resulting supernatant was removed, and 100% by volume of ethanol was added to the resulting pellet again to make a suspension. The suspension thus obtained was centrifuged again at 12000 rpm for 10 minutes. After centrifugation, the resulting supernatant was removed and the resulting pellet was dried. The pellet thus obtained will be hereinafter referred to as “dried pellet”.
To the dried pellet obtained from the surgically-resected sample, 700 μL of a solution containing 1% (w/v) SDS and 0.1 M sodium hydroxide was added. Also, to the dried pellet obtained from the biopsy sample, 500 μL of a solution containing 1% (w/v) SDS and 0.1 M sodium hydroxide was added. Each of the mixtures thus obtained was incubated at 100° C. for 20 minutes. After incubation, each of the mixtures was centrifuged at a temperature of 4° C. and at 12000 rpm for 10 minutes to collect the supernatant.
To the supernatant obtained from the surgically-resected sample, 700 μL of the bisulfite salt solution was added and mixed. Also, to the supernatant obtained from the biopsy sample, 500 μL of the bisulfite salt solution was added and mixed. Subsequently, each of the mixtures thus obtained was incubated at 80° C. for 40 minutes to carry out a bisulfite salt treatment. A nucleic acid contained in a solution after the bisulfite salt treatment was purified by a nucleic acid purification kit (manufactured by QIAGEN under the trade name of QIAquick PCR Purification Kit). To the nucleic acid thus obtained, sodium hydroxide was added so as to have a final concentration of 0.3 M. Subsequently, the mixture thus obtained was incubated at room temperature for 5 minutes. Thereafter, the product thus obtained was purified by a spin column for nucleic acid purification (manufactured by GE Healthcare under the trade name of MicroSpin S-300 HR Columns), whereby analytical samples were obtained.
A PCR reaction liquid was prepared by performing operations similar to those in Test Example 1, except that the analytical sample derived from the surgically-resected sample was used as an analytical sample. Also, a PCR reaction liquid was prepared by carrying out the same operations as those in Test Example 1, except that the analytical sample derived from the biopsy sample was used as an analytical sample and that the amount of the analytical sample was adjusted to 2 μL. As a primer set for the PCR reaction liquid, a primer set of Example 1 consisting of a primer consisting of the nucleotide sequence shown in SEQ ID NO: 10 and a primer consisting of the nucleotide sequence shown in SEQ ID NO: 11 was used.
The PCR conditions when the PCR reaction liquid containing the analytical sample derived from the surgically-resected sample is used are the same as those of PCR thermal profile (3) in Table 3. The PCR conditions when the PCR reaction liquid containing the analytical sample derived from the biopsy sample is used are conditions for carrying out the reaction of incubation at 95° C. for 4.5 minutes followed by 40 cycles of reactions, each cycle being 95° C. for 30 seconds, 60° C. for 15 seconds and 72° C. for 30 seconds.
PCR was carried out using the PCR reaction liquid under PCR conditions suitable for the kind of primer set, and the presence or absence of an amplification product was confirmed. The results are shown in
From the results shown in
Using a paraffin block of cancer tissue that was surgically resected from a subject and confirmed to have been infected with HPV18, and a paraffin block of uterine cervical tissue that was surgically resected from a subject and confirmed to have been infected with HPV58 and diagnosed as a tissue at a stage of CIN3 (surgically-resected sample) and a paraffin block of uterine cervical tissue derived from a patient that was collected by scratching the uterine cervix of a subject under colposcopic observation and confirmed to have been infected with HPV58 and in addition diagnosed to be at a stage of CIN3 (biopsy sample), an analytical sample of each of them was obtained by performing operations similar to those in Test Example 2.
To 2 μL of the analytical sample, 1.5 μL of reagent (×10 buffer) contained in a PCR reagent (manufactured by TAKARA BIO INC. under the trade name of TaKaRa EX Taq (trademark) Hot Start Version), 0.075 μL of DNA polymerase (trade name: TaKaRa Ex Taq HS (5 U/μL)), 1.2 μL of 2.5 mM dNTP mixture, 0.6 μL of an aqueous solution of forward primer (10 mM), 0.6 μL of an aqueous solution of reverse primer (10 mM), and 9.025 μL of water were added to prepare a PCR reaction liquid.
Primer sets each consisting of the forward primer and the reverse primer used for the analytical sample derived from the sample containing HPV18 are shown in Table 6. Primer sets each consisting of the forward primer and the reverse primer used for the analytical sample derived from the sample containing HPV58 are shown in Table 7.
Using the PCR thermal profile (6) used in Experimental Example 2 and each of the analytical samples derived from the surgically-resected sample and the biopsy sample as the PCR conditions, methylated CpG sites and unmethylated CpG sites in genomic DNA of each of HPV18 and HPV58 were analyzed by performing operations similar to those of Experimental Example 1. The results are shown in
From the results shown in
From the results, it is suggested that, regardless of the kinds of HPV, HPV causing onset of uterine cervical cancer can be detected by using the primer set consisting of the first primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine present in a site other than a CpG site is converted into another base in a nucleotide sequence having a CpG site in L1 region or L2 region of HPV, and the second primer which hybridizes with a nucleic acid consisting of a nucleotide sequence in which cytosine is converted into another base in a nucleotide sequence having a CpG site in LCR or E6 region of HPV. Also, it was suggested that, according to the primer set, whether or not a tissue obtained as a clinical specimen such as a surgically-resected sample and a biopsy sample is at a stage of high-grade dysplasia or a more severe stage can be determined.
A diagnostic kit for cancer caused by HPV or a diagnostic kit for a stage of dysplasia was prepared. One example thereof is shown below. The kit includes a nuclease-free container containing an aqueous solution of each primer of the below-described primer sets and a nuclease-free container containing a bisulfite salt solution (10M aqueous solution of sodium bisulfite), which is an unmethylated cytosine-conversion agent.
The content of the diagnostic kit for cancer caused by HPV or the diagnostic kit for a stage of dysplasia:
An aqueous solution of forward primer (an aqueous solution obtained by dissolving primer 16L1/LCR-F consisting of the nucleotide sequence shown in SEQ ID NO: 10 in nuclease-free water)
An aqueous solution of reverse primer (an aqueous solution obtained by dissolving the primer 16L1/LCR-F consisting of the nucleotide sequence shown in SEQ ID NO: 10 in nuclease-free water)
10M aqueous solution of sodium bisulfite
Cancer caused by HPV can be diagnosed easily with high accuracy by performing operations similar to those of Test Example 1 using the above kit. Also, the stage of dysplasia can be diagnosed easily with high accuracy by performing operations similar to those of Test Example 3 using the above kit.
SEQ ID No.: 1 is a sequence of a primer.
SEQ ID No.: 2 is a sequence of a primer.
SEQ ID No.: 3 is a sequence of a primer.
SEQ ID No.: 4 is a sequence of a primer.
SEQ ID No.: 5 is a sequence of a primer.
SEQ ID No.: 6 is a sequence of a primer.
SEQ ID No.: 7 is a sequence of a primer.
SEQ ID No.: 8 is a sequence of a primer.
SEQ ID No.: 10 is a sequence of a primer.
SEQ ID No.: 11 is a sequence of a primer.
SEQ ID No.: 12 is a sequence of a primer.
SEQ ID No.: 13 is a sequence of a primer.
SEQ ID No.: 14 is a sequence of a primer.
SEQ ID No.: 15 is a sequence of a primer.
SEQ ID No.: 17.is a sequence of a primer.
SEQ ID No.: 18 is a sequence of a primer.
SEQ ID No.: 19 is a sequence of a-primer.
SEQ ID No.: 20 is a sequence of a primer.
SEQ ID No.: 21 is a sequence of a primer.
SEQ ID No.: 22 is a sequence of a primer.
SEQ ID No.: 23 is a sequence of a primer.
SEQ ID No.: 24 is a sequence of a primer.
SEQ ID No.: 25 is a sequence of a primer.
SEQ ID No.: 26 is a sequence of a primer.
SEQ ID No.: 27 is a sequence of a primer.
SEQ ID No.: 28 is a sequence of a primer.
SEQ ID No.: 29 is a sequence of a primer.
SEQ ID No.: 30 is a sequence of a primer.
SEQ ID No.: 31 is a sequence of a primer.
SEQ ID No.: 32 is a sequence of a primer.
SEQ ID No.: 33 is a sequence of a primer.
SEQ ID No.: 34 is a sequence of a primer.
SEQ ID No.: 35 is a sequence of a primer.
SEQ ID No.: 36 is a sequence of a primer.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2008*074267 | Mar 2008 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP2009/055337 | 3/18/2009 | WO | 00 | 9/21/2010 |
