Patent Application
20210381978
The present invention relates to utilization of lanthanide time resolved fluorescence for measurement of silica species in water.
Silicon is naturally occurring element, existing in several forms, typically as silica, silicic acid and silicates. The general structure of silica is SiO2, whereas silicate family includes e.g. [SiO4]4- and [SiO3]2- anions. Silica or silicates polymerize also readily to oligomeric silicon-oxide compounds and further to polymeric silica depending on the water chemistry, such as silica concentration, other ions present and pH. Water soluble silica is typically mono- or oligomeric, whereas polymeric silica exists often as stable solid-liquid colloids in water. Precipitation of silica depends on numerous factors, such as pH, concentration and ion strength of the water. Silica scaling is extremely troublesome to treat due to its low solubility and strong adhesion on surfaces.
Precipitation of silica causes problems in several industries, such as in geothermal applications, cooling towers and desalination plants. Detection of soluble silica is extremely important as it enables efficient and in-time treatment of possible scaling problems.
Several methods, such as spectrometric, ion chromatography and colorimetric methods, for detecting silica have been developed. These methods, however, are often laborious and complex.
Therefore, there is still need for improved simple and effective methods for determining silica.
An object of the present invention is to provide a method for detecting silica in sample comprising silica.
Another object of the present invention is to provide a simple and efficient method for detecting silica in sample comprising silica.
In time-resolved resolved fluorescence (TRF) method desired signal is distinguished from the interfering, short-lived fluorescence signals by temporal resolution (the fluorescence signal is recorded after a certain length lag time).
Lanthanide ions exhibit several beneficial characteristics for TRF measurements: the fluorescence lifetimes of lanthanide ions are exceptionally long and they have narrow banded emission lines and long Stokes' shift.
Alone, lanthanide ions have very low energy absorption. The absorptivity of the lanthanides can be substantially increased by chelating the trivalent lanthanide ion with energy mediating ligands. In aqueous solutions, the ligands increase the absorptivity and protect the lanthanide ion from water molecules that quench the fluorescence signal by radiationless decay process of lanthanide and OH groups of water.
The inventors surprisingly found that anionic or acidic silica groups are able to chelate lanthanide cations and increase their time resolved (TRF) signal. This signal increase is utilized for quantification of silica/silicate species in water in the method of the present invention.
It was also surprisingly found the quantification can be enhanced by addition of additional lanthanide chelation agent, such as anionic polymer. Addition of anionic polymer was found to increase substantially the TRF signal of lanthanide, such as europium. The TRF signal increase of lanthanide(III)-silica was more pronounced when polymer was introduced into the measurement than in the absence of the polymer.
Presumably, without bounding to any theory, the additional chelation agent and orthosilica/oligomeric silica species chelate trivalent lanthanide cations together. The combination of different type chelation agents enable more efficient energy transfer and particularly protect the lanthanide cation from the lanthanide-water interactions more efficiently than they do separately. Thus, even smaller concentrations of silica in a sample can be detected.
The present invention relates to a method for determining concentration of silica in a sample. More particularly the present invention relates to a method for determining concentration of silica in a sample comprising silica, the method comprising
In a preferred embodiment additionally a lanthanide chelating agent or chelating agents is/are admixed with the sample prior exciting the sample.
In one embodiment the reagent comprising lanthanide(III) ion and the chelating agent or agents are admixed together prior admixing with the sample.
In other embodiment the sample and the chelating agent or agents are admixed together prior admixing with the reagent comprising lanthanide(III) ion.
In other embodiment the reagent comprising lanthanide(III) ion and the sample are admixed together prior admixing with the chelating agent or agents.
The chelating agent comprises at least one or more functional groups capable of chelating lanthanide(III) ions. Preferably the one or more one functional groups are selected from esters, ethers, thiols, hydroxyls carboxylates, sulfonates, amides, phosphates, phosphonates, amines or any combination thereof.
In an embodiment, chelating agent contains additionally aromatic group or groups. The aromatic group(s) amplifies the signal of the lanthanide(III) ion.
The lanthanide(III) ion is selected from europium, terbium, samarium or dysprosium ions, preferably europium or terbium ions.
In a preferred embodiment the reagent comprises lanthanide(III) salt. The lanthanide(III) salt is selected from halogenides and oxyanions, such as nitrates, sulfates or carbonates, preferably from hydrated halogenides or nitrates, more preferably chloride.
The silica in the sample can be any suitable silica. Preferably the silica comprises silicic acid or oligomeric silicate soluble in water.
In one embodiment concentration of the silica in the measurement mixture is in the range of 0.1-100 ppm, preferably 0.5-50 ppm, and more preferably 1-30 ppm.
In case the concentration of the silica in the sample is higher, the sample can be diluted.
In another embodiment concentration of the lanthanide(III) ion in the measurement mixture is in the range of 0.1-100 μM, preferably 0.1-50 μM, and more preferably 1 μM 20 μM.
In preferred embodiment concentration of the lanthanide chelating agent in the measurement mixture is in the range of 0.01-500 ppm, preferably 0.5-50 ppm, and more preferably 0.5-20 ppm.
By term “measurement mixture” is meant the admixture in the measurement.
The sample is optionally diluted to suitable aqueous solution e.g. deionized water or brine containing monovalent and/or divalent ions. Preferably, the dissolution brine does not contain any trivalent ions. Preferably the sample is an aqueous solution.
If the sample solution contains some interfering compounds such as trivalent metal cations or chelating agents that may affect TRF signal, suitable purification procedures may be applied prior to the dilution steps.
The sample is optionally purified by using a purification method selected from centrifugation, size exclusion chromatography, cleaning with solid-phase extraction (SPE) cartridges, dialysis techniques, extraction methods for removing hydrocarbons, filtration, microfiltration, ultrafiltration, nanofiltration, membrane centrifugation and any combinations thereof.
In one embodiment pH value of the sample is adjusted to a level in range between pH 3 and pH 8, preferably in range from pH 5 to pH 8
Unknown concentration of the silica in the sample is determined by comparing the sample signal to calibration curve. The calibration curve is obtained from TRF measurement of calibration standard samples with varying silica concentrations and the optional chelating agent in fixed concentration. Same dilution and/or purification steps and measurement parameters have to be used for both the sample and calibration samples.
The lanthanide(III) ion is excited at excitation wavelength and measured at emission wavelength and detected by using time-resolved fluorescence (TRF). Any TRF reader can be employed. Excitation and emission wavelengths are selected so that the S/N is the best. Also the delay time can be optimized.
The excitation and emission wavelengths and the delay time are chosen based on the requirements of the lanthanide ion.
In an exemplary embodiment excitation wavelength and emission wavelength and delay time for Europium is 395 nm and 615 nm and 400 μs respectively.
The present invention further relates to use of the method of the present invention for determining concentration of silica in a sample.
The sample can originate from originates from geothermal processes, cooling towers, desalination plants and water treatment process.
The present invention further relates a device comprising means for performing the method according to the present invention for determining concentration of silica in a sample.
The examples are not intended to limit the scope of the invention but to present embodiments of the present invention.
All the reagents were diluted into brine, which composition is presented in Table 1. EuCl3.6 H2O was used as lanthanide source. The europium salt was diluted into brine so that the concentration of europium was 22.48 μM. Silica sample solution was prepared by diluting sodium silicate (Na2SiO3) into the brine. The Na2SiO3 concentration was varied between 0 and 30 ppm. 100 μl of both lanthanide and silica solutions were pipetted into microplate (MICROPLATE BIOCHEM 96WELL BLACK), and the TRF signal of the mixture was measured using Tecan Spark multiplate reader. The lag time, excitation and emission wavelengths used were 400 μs, 295 nm and 615 nm, respectively.
All the reagents were diluted into brine, which composition is presented in Table 1. EuCl3.6 H2O was used as lanthanide source. The europium salt was diluted into brine so that the concentration of europium was 22.48 μM. 200 ppm chelating agent solution was prepared by diluting the chelating agent into brine. Polyacrylic acid type polymer can be used as chelating agent. Silica sample solution was prepared by diluting sodium silicate (Na2SiO3) into the brine. The Na2SiO3 concentration was varied between 0 and 120 ppm. 100 μl of lanthanide solution was first pipetted into microplate (MICROPLATE BIOCHEM 96WELL BLACK), after which 50 μl of silica solution and 50 μl of chelating agent were added to the plate. The TRF signal of the mixture was measured using Tecan Spark multiplate reader. The lag time, excitation and emission wavelengths used were 400 μs, 295 nm and 615 nm, respectively.
| Number | Date | Country | Kind |
|---|---|---|---|
| 20185820 | Oct 2018 | FI | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/FI2019/050692 | 9/27/2019 | WO | 00 |
