Patent Application
20160091428
This application claims priority from prior Japanese Patent Application No. 2014-202184, filed on Sep. 30, 2014, entitled “Method for determining abnormality in particle analyzer, method for performing quality control in analyzer, and particle analyzer”, the entire content of which is incorporated herein by reference.
The present invention relates to a method for determining abnormality in a particle analyzer and a particle analyzer.
There are particle analyzers which analyze particles by detecting fluorescence generated from the particles. Such a particle analyzer mixes a sample containing particles with a fluorescent dye to prepare a measurement specimen, and causes the prepared measurement specimen to flow in a flow cell. The particle analyzer irradiates the flow of the measurement specimen with light, to detect fluorescence generated from each particle. Based on signals obtained through this detection, the particles are classified. In such a particle analyzer, in order to obtain an accurate measurement result, quality control using a quality control standard material is performed.
With the technique according to U.S. Patent Application Publication No. 2007/013906, the place of the abnormality occurring in the particle analyzer is determined by use of a quality control substance which contains: first standard particles to be fluorescence-stained by a first fluorescent dye; and second standard particles which contain a second fluorescent dye in advance and which are substantially not to be stained by the first fluorescent dye. When the measurement result of the second standard particles is outside a reference range, it is determined that abnormality is in the fluorescence detector. In the case where the measurement result of the second standard particle is within the reference range, when the measurement result of the first standard particle is outside a reference range, it is determined that abnormality is in the specimen preparation mechanism. In the case where the measurement result of the first standard particle is smaller than its reference range, and the measurement result of the second standard particle is greater than its reference range, it is determined that abnormality is in the specimen preparation mechanism and in the fluorescence detector.
The scope of the present invention is defined solely by the appended claims, and is not affected to any degree by the statements within this summary.
With the technique according to U.S. Patent Application Publication No. 2007/013906, there are cases whether abnormality is in the staining step or not cannot be discerned.
A first aspect of the present invention relates to a method for determining abnormality in a particle analyzer. The method includes: staining first control particles but not staining second control particles which emit fluorescence; irradiating with light the first control particles and the second control particles flowing in a flow cell, and detecting fluorescence from the first control particles and the second control particles; obtaining a first management value indicating a detection result of the fluorescence emitted from the first control particles and a second management value indicating a detection result of the fluorescence emitted from the second control particles; and determining abnormality in the staining step, based on a value calculated from the first management value and the second management value or a ratio between the first management value and the second management value.
A second aspect of the present invention relates to a method for determining abnormality in a particle analyzer which analyzes, based on a signal waveform of fluorescence emitted from a fluorescence-stained cell nucleus, an amount of DNA contained in the cell nucleus. The method includes: staining first control particles but not staining second control particles which emit fluorescence; irradiating with light the first control particles and the second control particles flowing in a flow cell, and obtaining signal waveforms based on the fluorescence from each first control particle and each second control particle; obtaining an area of the signal waveform of the fluorescence from each first control particle as a first fluorescence area; obtaining an area of the signal waveform of the fluorescence from each second control particle as a second fluorescence area; and determining abnormality in staining performed in the particle analyzer, based on the first fluorescence area and the second fluorescence area.
A third aspect of the present invention relates to a particle analyzer including: a specimen preparation unit configured to mix a specimen and a reagent which contains a fluorescent dye; an optical detection unit configured to cause the mixture prepared by the specimen preparation unit to flow in a flow cell, configured to irradiate with light the mixture flowing in the flow cell, and configured to detect light generated from the mixture as a result of the irradiation; and an analysis unit configured to analyze characteristic of light detected by the optical detection unit. When a quality control mode is set, the analysis unit is configured to cause the specimen preparation unit to mix the reagent, first control particles which are to be stained by the fluorescent dye, and second control particles which are not to be stained by the fluorescent dye and which emit fluorescence, to prepare a mixture, cause the optical detection unit: to cause the mixture to flow in the flow cell; to irradiate with light the first control particles and the second control particles flowing in the flow cell; and to detect fluorescence from the first control particles and the second control particles, obtain a first management value indicating a detection result of the fluorescence emitted from the first control particles and a second management value indicating a detection result of the fluorescence emitted from the second control particles, and determine abnormality in staining performed in the specimen preparation unit, based on a value calculated from the first management value and the second management value or a ratio between the first management value and the second management value.
The effects and the significance of the present invention will be further clarified by the description of the embodiments below. However, the embodiments below are merely examples for implementing the present invention, and the present invention is not limited to the embodiments below by any degree.
Embodiments 1 and 2 below are applied to an apparatus whose analysis target is uterine cervix cells and which obtains information regarding canceration of the cells. The analysis target may be buccal cells, epithelial cells of the bladder, the pharynx, or the like, or epithelial cells of organs.
As shown in
First, a case where the operation mode is the normal mode will be described. The sample 11 is a liquid in which cells are suspended in a preservative liquid whose principal component is alcohol. The sample 11 is contained in a sample container. Preferably, the alcohol is methanol. The transfer unit 20 aspirates the sample 11, dispenses the aspirated sample 11 into a cuvette not shown, and transfers the cuvette. The pretreatment unit 30 disperses, with ultrasonic waves, aggregated cells contained in the sample 11 dispensed in the cuvette. Further, the pretreatment unit 30 replaces the preservative liquid suspending the cells contained in the sample 11, with a diluent. The pretreatment unit 30 removes contaminant from the sample 11 and concentrates the sample 11.
The specimen preparation unit 40 mixes reagents 41 and 42 and the sample 11 having been subjected to the processing by the pretreatment unit 30, to prepare a mixture. The reagent 41 contains a fluorescent dye. The fluorescent dye contained in the reagent 41 is a nucleic acid staining dye. Nucleic acid in each cell contained in the sample 11 is stained by the reagent 41.
It is sufficient that the nucleic acid staining dye is a fluorescent dye which emits fluorescence by binding to nucleic acid. An example of the nucleic acid fluorescent dye is propidium iodide, ethidium bromide, ethidium-acridine heterodimer, ethidium diazide, ethidium homodimer-1, ethidium homodimer-2, ethidium monoazide, trimethylenebis[[3-[[4-[[(3-methyl benzothiazole-3-ium)-2-yl]methylene]-1,4-dihydroquinoline]-1-yl]propyl]dimethylaminium]tetraiodide, 4-[(3-methylbenzothiazole-2(3H)-ylidene)methyl]-1-[3-(trimethylaminio) propyl]quinolinium diiodide, N,N,N′,N′-tetramethyl-N,N′-bis[3-[4-[3-[(3-methylbenzothiazole-3-ium)-2-yl]-2-propenylidene]-1,4-dihydroquinoline-1-yl]propyl]-1,3-propanediaminium tetraiodide, or 2-[3-[[1-[3-(trimethylaminio) propyl]-1,4-dihydroquinoline]-4-ylidene]-1-propenyl]-3-methylbenzothiazol-3-ium diiodide.
The reagent 42 contains an RNA remover for removing RNA contained in the sample 11. The sample 11 sometimes contains RNA in the cells. Since the reagent 41 is a nucleic acid staining dye, there are cases where the reagent 41 stains RNA contained in the sample 11. When RNA is stained, RNA-derived fluorescence is added to DNA-derived fluorescence to be detected, causing increased background, which is not preferable. Such RNA is degraded by the reagent 42.
The optical detection unit 50 includes a flow cytometer. The optical detection unit 50 causes the mixture prepared by the specimen preparation unit 40 to flow in a flow cell 50a. The optical detection unit 50 irradiates with light the mixture flowing in the flow cell 50a. The optical detection unit 50 detects light emitted from the mixture.
As shown in
The light source 51 emits a laser beam. The condenser lens group 52 is composed of a plurality of lenses. The condenser lens group 52 condenses the laser beam on the mixture flowing in the flow cell 50a. Accordingly, from the particles in the mixture, forward scattered light and fluorescence are generated. Forward scattered light reflects the size of each particle, and fluorescence reflects the degree of staining of the particle.
The condenser lens 53 condenses forward scattered light. The optical detector 54 receives forward scattered light. The optical detector 54 is a photodiode. The optical detector 54 outputs an electric signal corresponding to the received forward scattered light, i.e., a forward scattered light signal. The condenser lens 55 condenses fluorescence. The optical detector 56 receives fluorescence. The optical detector 56 is a photomultiplier. The optical detector 56 outputs an electric signal corresponding to the received fluorescence, i.e., a fluorescence signal.
The signal processing circuit 57 performs predetermined signal processing on signals outputted from the optical detectors 54 and 56, to obtain waveforms corresponding to the forward scattered light signal and the fluorescence signal, respectively. The signal processing circuit 57 calculates a plurality of characteristic parameters such as the peak value, the width, and the area, from each obtained waveform. As shown in
With reference back to
Next, a case where the operation mode is the quality control mode will be described. The first control particles 12a are particles to be stained by the fluorescent dye of the reagent 41. The second control particles 12b are particles which are substantially not stained by the fluorescent dye of the reagent 41, and which emit fluorescence by containing a fluorescent dye in advance.
The first control particles 12a are selected from the group consisting of: cells, polyacrylamide particles, hydrophilic vinyl polymer particles, latex particles, and silica particles. Preferably, the first control particles 12a are cells.
The second control particles 12b are fluorescent latex particles. The second control particles 12b emit fluorescence more intense than that emitted by the first control particles 12a. More specifically, the second control particles 12b emit fluorescence more intense than that emitted by the first control particles 12a which have been stained under an appropriate condition by use of the reagents 41 and 42. Thus, based on the difference between the fluorescence intensities, the first control particles 12a can be distinguished from the second control particles 12b.
The first control particles 12a and the second control particles 12b are contained in containers, respectively. The transfer unit 20 aspirates the first control particles 12a and the second control particles 12b, dispenses them into cuvettes not shown, and transfers the cuvettes. The pretreatment unit 30 is not used in the quality control mode. The specimen preparation unit 40 mixes the first control particles 12a, the second control particles 12b, and the reagents 41 and 42, to prepare a mixture. Accordingly, the first control particles 12a are stained by the reagent 41.
The first control particles 12a contain RNA in the specimen. This is for determining whether the reagent 42 containing the RNA remover is properly acting or not.
As in the normal mode, the optical detection unit 50 causes the mixture prepared by the specimen preparation unit 40 to flow in the flow cell 50a. The optical detection unit 50 irradiates with light the first control particles 12a and the second control particles 12b flowing in the flow cell 50a. The optical detection unit 50 detects light emitted from each of the first control particles 12a and the second control particles 12b. The signal processing circuit 57 of the optical detection unit 50 outputs, to the analysis unit 60, characteristic parameters of each light of each particle. The analysis unit 60 stores, in the storage 60a, the characteristic parameters of each light of each particle outputted from the signal processing circuit 57.
Based on the characteristic parameters of each light of each particle, the analysis unit 60 obtains a first management value, a second management value, and a third management value. The first management value and the second management value represent detection results of fluorescence emitted from the first control particles 12a and the second control particles 12b, respectively. The third management value is a value calculated from the first management value and the second management value. Based on the second management value, the analysis unit 60 determines detection abnormality, i.e., abnormality in the optical detection unit 50. Based on the third management value, the analysis unit 60 determines staining abnormality, i.e., abnormality in staining performed in the specimen preparation unit 40. When there are detection abnormality and staining abnormality, the analysis unit 60 outputs notifications of the respective detection abnormality and staining abnormality via the output unit 70. How to determine detection abnormality and staining abnormality will be described later with reference to flow charts.
Next, the process performed by the particle analyzer 10 in the normal mode will be described with reference to the flow chart shown in
As shown in
In step S105, based on the characteristic parameters of each light of each particle stored in the storage 60a, the analysis unit 60 extracts cells whose N/C ratio is within a predetermined range from among all the cells contained in the mixture. By calculating “the width of the waveform of a fluorescence signal/the width of the waveform of a forward scattered light signal”, the analysis unit 60 obtains the N/C ratio. Instead of the N/C ratio, the analysis unit 60 may calculate the C/N ratio, and may extracts cells whose C/N ratio is within a predetermined range.
Next, in step S106 and S107, the analysis unit 60 performs analysis based on the amount of DNA of each of cells contained in the sample 11 as described below. In step S106, the analysis unit 60 creates a histogram regarding the amount of DNA of the cells extracted in step S105. Specifically, the analysis unit 60 creates a histogram whose two axes represent the area of waveform of fluorescence signal and the number of cells. The area of the waveform of a fluorescence signal (hereinafter, referred to as “fluorescence area”) corresponds to the amount of DNA of the cell. In step S107, the analysis unit 60 analyses the fluorescence areas of the cells contained in the sample 11, to obtain the ratio of cells each containing a predetermined amount or more of DNA (hereinafter, referred to as “target cell ratio”). Specifically, based on the histogram created in step S106, the analysis unit 60 calculates “the number of cells each having a fluorescence area greater than or equal to a predetermined threshold/the number of cells each having a fluorescence area less than the predetermined threshold”, to obtain a target cell ratio.
Here, for convenience of explanation, the target cell ratio is obtained by creating a histogram in step S106, but it is not necessary to actually create a histogram. That is, the target cell ratio may be obtained through data processing based on the cells extracted in step S105. Similarly, also in step S204 in
In step S108, based on the target cell ratio obtained in step S107, the analysis unit 60 determines whether retesting is necessary or not. When the target cell ratio is greater than or equal to a predetermined value, the analysis unit 60 determines that retesting is necessary. When the target cell ratio is less than the predetermined value, the analysis unit 60 determines that retesting is not necessary. In step S109, the analysis unit 60 displays, on the output unit 70, the target cell ratio and the determination result indicating whether retesting is necessary or not, as the analysis result.
In the particle analyzer 10 which performs analysis based on the amount of DNA, staining of nucleic acid by the reagent 41 and reduced background by the reagent 42 have direct influence on the shape of the histogram regarding the amount of DNA. The reagent 41 and the reagent 42 may deteriorate due to storage condition and use condition. When the reagent 41 is deteriorated, nucleic acid is not sufficiently stained. This may case the distribution of the histogram to be shifted toward the low value side. When the reagent 42 is deteriorated, RNA is not sufficiently removed, and background of fluorescence is increased. This may cause the distribution of the histogram to be shifted toward the high value side. Either case could have unfavorable influence on accurate analysis of the amount of DNA.
Therefore, quality management of the reagents 41 and 42 is important, and further, it is important to determine staining abnormality in the specimen preparation unit 40 in the quality control mode.
Next, the process performed by the particle analyzer 10 in the quality control mode will be described with reference to the flow chart shown in
As shown in
In step S204, the analysis unit 60 obtains a first measurement value and a second measurement value. Specifically, first, based on the difference in fluorescence intensities, the analysis unit 60 classifies data of each particle stored in the storage 60a, as either the first control particle 12a or the second control particle 12b. As described above, the second control particles 12b emit fluorescence more intense than that emitted by the first control particles 12a. Therefore, based on the difference in fluorescence intensities, classification between the first control particles 12a and the second control particles 12b can be performed.
Next, with respect to each type of the first control particles 12a and the second control particles 12b, as shown in
In step S205, based on a predetermined number of times of measurements, the analysis unit 60 determines whether the quality control measurement has ended. When it is assumed that the predetermined number of times of measurements is n, the analysis unit 60 repeats the processes of steps S202 to S204 n times. When it is assumed that the n-th first measurement value is V1n and the n-th second measurement value is V2n, the analysis unit 60 obtains a first measurement value and a second measurement value in each of the first to n-th measurements as shown in
In the above procedure, the reagents 41 and 42, the first control particles 12a, and the second control particles 12b are mixed together to prepare a mixture. However, instead of this, a mixture of the first control particles 12a and a mixture of the second control particles 12b may be separately prepared. In this case, the processes of steps S202 to S205 are replaced with steps S221 to S228, as shown in
As shown in
Next, in steps S225 to S228, as in steps S221 to S224, the analysis unit 60 obtains the second measurement values the predetermined number of times. Also through steps S221 to S228 in
With reference back to
First management value=(V11+V12+ . . . +V1n)/n (11)
Second management value=(V21+V22+ . . . +V2n)/n (12)
Third management value=first management value/second management value (13)
The second management value is a value obtained based on fluorescence generated from the second control particles 12b which are substantially not stained by the reagent 41 and which contain a fluorescent dye in advance. Thus, the second management value reflects the state of the optical detection unit 50. In contrast, the first management value is a value that also reflects the state (such as reagent deterioration) of the staining step in addition to the state of the optical detection unit 50. The first management value can be considered as the product of the second management value representing the state of the optical detection unit 50 and the value representing the state of the staining step. Therefore, the third management value obtained by dividing the first management value by the second management value reflects the state of the staining step.
The third management value may be a value obtained by squaring the first management value/the second management value. The third management value may be a value obtained by adding a constant to the first management value/the second management value, subtracting a constant from the first management value/the second management value, multiplying the first management value/the second management value with a constant, or dividing the first management value/the second management value by a constant. The third management value may be the second management value/the first management value. The third management value may be a value of a log of the first management value/the second management value. The third management value may be obtained by (V11+V12+ . . . +V1 n)/(V21+V22+ . . . +V2n), without using the first management value and the second management value. The third management value may not be a value based on the ratio between the first management value and the second management value. For example, the ratio of the first management value to a target value 1 of the first management value (first management value/target value 1) and the ratio of the second management value to a target value 2 of the second management value (second management value/target value 2) are calculated, and then, the difference between these ratios may be used as the third management value.
In step S207, based on the second management value and the third management value, the analysis unit 60 performs the processes of abnormality determinations shown in
As shown in
As shown in
The staining abnormality determination may be a process shown in
As shown in
Here, for convenience of explanation, in step S421, a point based on the first management value and the second management value is plotted on the coordinate space, but it is not necessary to actually create the coordinate space and plot the point. That is, instead of creating the coordinate space, plotting the point, and determining whether the plotted point is within the normal range, whether the point based on the first management value and the second management value is within the normal range may be determined by data processing.
The process of abnormality determination is performed in accordance with separate flow charts as shown in
With reference back to
When having determined as NO in step S208 and having determined as NO in step S209, then, in step S211, the analysis unit 60 displays a screen 71 as shown in
When having determined as NO in step S208 and having determined as YES in step S209, then, in step S212, the analysis unit 60 displays a screen 72 as shown in
In step S213, the analysis unit 60 prohibits measurement on the sample 11 in the normal mode. Accordingly, the processes of step S102 and thereafter in
When having determined as YES in step S208 and having determined as NO in step S210, then, in step S214, the analysis unit 60 displays a screen 73 as shown in
When having determined as YES in step S208 and having determined as YES in step S210, then, in step S215, the analysis unit 60 displays a screen 74 as shown in
In step S215, even in the case where there is abnormality in both the optical detection unit 50 and the specimen preparation unit 40, notifications of detection abnormality and reagent abnormality are individually and assuredly made. Accordingly, the operator can smoothly and quickly take measures for eliminating the abnormalities, and thus, the operator can smoothly and quickly eliminate the detection abnormality and the reagent abnormality.
Every time the analysis unit 60 obtains a third management value in the quality control mode, the analysis unit 60 may store the obtained third management value in the storage 60a. In this case, by observing the change in the third management value over time, it is possible to predict deterioration of the reagents 41 and 42, and to predict replacing time of the reagents 41 and 42.
In Embodiment 2, the configuration of the particle analyzer 10 is the same as that in Embodiment 1, and only the process of staining abnormality determination is different from that in Embodiment 1, as described below.
As shown in
When having determined as NO in step S441 and having determined as NO in step S442, then, in step S443, the analysis unit 60 determines that there is no staining abnormality. When having determined as NO in step S441 and having determined as YES in step S442, then, in step S444, the analysis unit 60 determines that there is abnormality in the reagent 41. When having determined as YES in step S441, then in step S445, the analysis unit 60 determines that there is abnormality in the reagent 42. The analysis unit 60 may perform the determination in steps S441 and S442 by use of the coordinate space shown in
When it has been determined that there is abnormality in a reagent in step S444, S445, a message urging replacement of the reagent is displayed in a later stage of step S212, S215 in
Here, the case where it is determined that there is staining abnormality in the process of the staining abnormality determination in Embodiment 1 shown in
Thus, according to Embodiment 2, as in Embodiment 1, it is possible to determine that there is staining abnormality, and further, it is possible to know which of the reagents 41 and 42 should be replaced.
<Experiment Regarding Abnormality Determination>
Next, an experiment actually conducted regarding abnormality determination will be described.
1. Material
The first control particles 12a, the second control particles 12b, the reagent 41, and the reagent 42 used in the experiment were as follows.
The first control particles 12a were prepared as follows. C33A cells (HTB-31) obtained from American Type Culture Collection were suspended in PreservCyt (registered trademark) of Hologic Inc. and left still for 24 hours. Then, the mixture was centrifuged to remove the supernatant. The resultant mixture was suspended in pH7.5, 10 mM Tris hydrochloric acid aqueous solution. This final mixture was used as the first control particles 12a. As the second control particles 12b, AlignFlow Plus Flow Cytometry Alignment Beads (A-7303) of Life Technologies Inc. diluted by pH7.5, 10 mM Tris hydrochloric acid aqueous solution were used.
As the reagent 41, propidium iodide (PI) of Sigma-Aldrich Co. LLC. diluted by ethylene glycol was used. The reagent 41 was prepared in two types. That is, after the reagents 41 were prepared, one was stored with light blocked and the other was stored under exposure to light. Thus, the reagent 41 stored with light blocked, and the reagent 41 stored under exposure to light to be deteriorated were used as the reagent 41. As the reagent 42, RNaseA (Cat. R4642) of Sigma-Aldrich Co. LLC. diluted by pH7.5, 10 mM Tris hydrochloric acid aqueous solution was used.
2. Condition
In the experiment, in order to reproduce abnormality in the reagent 41 being a stain liquid and sensitivity abnormality in the optical detection unit 50, the reagent 41 and the voltage applied to the optical detector 56 which detects fluorescence were changed in accordance with the following three conditions.
Condition 1: the reagent 41 was the one stored under exposure to light; and the voltage applied to the optical detector 56 was 251 V, which was an appropriate voltage.
Condition 2: the reagent 41 was the one stored under exposure to light; and the voltage applied to the optical detector 56 was 255 V.
Condition 3: the reagent 41 was the one stored with light blocked; and the voltage applied to the optical detector 56 was 253 V.
3. Experiment Procedure
In the experiment, measurement regarding the first control particles 12a and measurement regarding the second control particles 12b were separately performed. That is, in the flow chart shown in
In step S221 in
Next, in step S225 of
The first measurement values and the second measurement values obtained under Conditions 1 to 3 are shown in the upper tables in
Next, in step S206 in
The upper limit thresholds and the lower limit thresholds used in determination on the first to third management values were set as shown in
Next, system abnormality determination, detection abnormality determination, and staining abnormality determination were performed. The detection abnormality determination and the staining abnormality determination were performed in accordance with the processes shown in
4. Experiment Result
As shown in the lower tables in
The experiment above has revealed that, under Condition 1 using the reagent 41 stored under exposure to light, even though the determinations on the first management value and the second management value were both “G”, i.e., there was no abnormality in the system and the detection, the determination on the third management value was “NG”, i.e., there was abnormality in staining. This abnormality is the one that cannot be found by the technique of U.S. Patent Application Publication No. 2007/013906 which focuses only on the amount of fluorescence of the first control particles 12a (first management value) and the amount of fluorescence of the second control particles 12b (second management value).
In this point, according to the techniques in Embodiments 1 and 2, the third management value representing the staining state is obtained separately from the first management value, whereby it is possible to find abnormality in the staining step which was not grasped conventionally. By replacing the relevant reagent to cause the third management value to be within the reference range, the operator can perform maintenance such that appropriate DNA amount analysis can be performed.
In Conditions 2 and 3, the determinations on the first management value were the same with each other, and the determinations on the second management value were the same with each other. However, under Condition 2 using the reagent 41 stored under exposure to light, the determination on the third management value was “NG”, i.e., that there was abnormality in staining was shown. Under Condition 3 using the reagent 41 stored with light blocked, the determination on the third management value was “G”, i.e., that there was no abnormality in staining was shown. The difference in the determination between Condition 2 and Condition 3 cannot be found either, by the technique of U.S. Patent Application Publication No. 2007/013906 which focuses only on the amount of fluorescence of the first control particles 12a (first management value) and the amount of fluorescence of the second control particles 12b (second management value).
The technique of U.S. Patent Application Publication No. 2007/013906 does not provide indices for determining a case where the second management value indicates abnormality, i.e., a case there is abnormality in the optical detection unit, and further, for determining whether there is abnormality also in the staining step. In the case where there is abnormality in both of the optical detection unit and the staining step, the operator performs reagent replacement in addition to sensitivity adjustment of the optical detection unit. However, with the conventional technique, whether the abnormality requires reagent replacement is unknown.
In this point, according to the techniques of Embodiments 1 and 2, based on the third management value which is the value of the ratio of the first management value to the second management value, detection abnormality and staining abnormality can be separately determined. Therefore, it is seen that staining abnormality that cannot be determined from the first management value and the second management value can be determined by use of the third management value. Thus, it is seen that, according to Embodiments 1 and 2, even in the case where there is abnormality in both of the optical detection unit 50 and the specimen preparation unit 40, it is possible to individually and assuredly make notifications of the detection abnormality and the reagent abnormality.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2014-202184 | Sep 2014 | JP | national |
