Patent Application
20070111206
A multipurpose diagnostic methodology for bacterial infections including tuberculosis is described that includes processing of clinical specimens and the utilization of the processed material for smear microscopy, culture and polymerase chain reaction. It is applicable for all types of pulmonary and extra pulmonary tuberculosis.
Tuberculosis kills more people in India and South-East Asia than any other infectious disease—more than HIV, STD, malaria and tropical diseases combined. In India more than one thousand people die from TB everyday—more than 450,000 per year, 1 every minute. Rapid diagnosis of tuberculosis is becoming increasingly important to improve cure rates, reduce the risk of spreading the disease through pulmonary route, and to combat the recent emergence of drug resistant strains of M. tuberculosis bacteria and its severe implications in HIV-infected patients. The causative organism of the disease is Mycobacteriun tuberculosis. This organism is characterized by its slow growth and the presence of a unique lipid-rich cell wall that makes it unamenable to the action of conventional antibiotics and lytic procedures. The acid-fast properties of the cell wall are utilized to rapidly detect the presence of M. tuberculosis and other mycobacteria in clinical specimens by smear microscopy.
By far smear microscopy is the most popular amongst all methods currently employed worldwide in the laboratory diagnosis of tuberculosis on account of its simplicity, speed, low cost and minimal requirement for equipment and technical skill. However it suffers from lack of sensitivity since a load of ˜10,000 organisms/ml of specimen is required to give a positive report by Ziehl Neelsen staining from concentrated samples (Kent and Kubica, 1995). A more sensitive smear microscopy method that is also simple would be highly useful in diagnostic laboratories. Culture is considered as the gold standard, but on account of the slow growth rate of the pathogen, sole dependence on it would invariably lead to a delay of 46 weeks in arriving at a definitive diagnosis. Apart from pulmonary tuberculosis, extra pulmonary tuberculosis presents an enormous diagnostic challenge on account of the wide spectrum of organs involved and the paucibacillary load of bacteria in the sample sent to the diagnostic laboratory.
Nucleic acid amplification techniques were championed as providing alternative sensitive, specific and rapid approaches to tuberculosis diagnosis. These approaches have suffered in no small measure from the inability to isolate mycobacterial PCR-amplifiable DNA of adequate quality from all types of clinical material. To the best of our knowledge a DNA isolation method using environment-friendly reagents that is universally applicable on all types of clinical material is currently not available. This presents a very practical and serious impediment to the application of DNA amplification methodologies to clinical material.
Against this background, a single universal methodology has been developed which includes a unique sample processing technique that alone is compatible with the most commonly used laboratory methods of TB diagnostics, namely, smear microscopy, culture and PCR. It is applicable on all types of clinical material except fecal matter, in pulmonary and extra pulmonary cases of tuberculosis. The method takes advantage of the unique properties of the mycobacterial cell wall to selectively remove extraneous materials, contaminating organisms and potential PCR-inhibitory material without adverse effect on M. tuberculosis acid-fast properties, viability and integrity. The chaotropic properties of guanidinium hydrochloride are utilized for this purpose in conjunction with detergents and neutral pH buffer.
Smear microscopy and culture are the most widely used laboratory techniques for the diagnosis of tuberculosis worldwide. In recent years, nucleic acid-based approaches, PCR in particular, are also being employed as an adjunct test to arrive at a definitive diagnosis on a reasonable period of time. To the best of our knowledge, published techniques and commercial kits for smear microscopy, culture and PCR along with their shortcomings are as follows:
Smear microscopy and culture: The methods for preparing samples for smear microscopy and cultivation of M. tuberculosis, involve the use of 4% NaOH; 0.5% NALC and 2% NaOH (3% NaOH is recommended for tropical countries with highly humid climates); dithiothreitol (DTT) and 2% NaOH; 13% trisodium phosphate with or without benzalkonium chloride (Zephiran); 5% oxalic acid; 1% cetylpyridium chloride and 2% NaCl. (Koneman et al. 1997). Household bleach (NaOCl) has also been used for liquefaction of sputum and preparation of smears. It requires overnight precipitation and smearing from sediment. In 3 studies performed in Ethiopia and India, the use of the NaOCl method increased the number of samples positive for acid-fast bacilli by more than 100% (Gebre et al. 1995).
But some studies have demonstrated that though bleach sedimentation can increase the diagnostic yield, it is only to a minor extent (Van Deun et al. 2000). The basic aim of each of the methods is to liquefy and decontaminate the sample and have been most widely used for sputum samples. NALC and DTT are effective mucolytic agents but are costly. Decontaminants like NaOH and trisodium phosphate require that the exposure time be controlled carefully. Zephiran requires neutralization with lecithin and is not compatible with inoculation on egg-based culture media. (Koneman. et al., 1997).
Recently a method for processing respiratory specimens by using C18-Carboxypropylbetaine (CB-18™), a zwitterionic detergent has been described for detection of mycobacteria. The method involves mild alkaline digestion of the sample and treatment with CB-18™ followed by an incubation of 2-24 hours, centrifugation and smearing for AFB. For culturing of M. tuberculosis a separate protocol is prescribed wherein three lytic enzymes are used in conjunction with CB-18™ which increases the cost. The initial study showed that the aggregate smear sensitivity and culture sensitivity increases by approximately 58% and 43% respectively by this method when compared to the NALC—NaOH method (Thornton et al. 1998).
In a recent report where the CB-18™ method was compared with the NALC—NaOH method, the former method showed a smear sensitivity of 59.6% that later increased to 71.4% on modification of the protocol (Scott et al. 2002). It is to be noted that sample processing by this method is not compatible with culturing in liquid media. This product is marketed by Quest Diagnostics Incorporated, Baltimore, USA as CB-18™ Smear Kit and CB-18™ TB Culture Kit with Lytic DECON II.
Another method using chitin for mucus digestion in sputum has been described recently which involves the addition of chitin solution to the sputum, homogenization by vortexing or shaking, sedimentation at unit gravity for 30 minutes and preparation of smear from the sediment. This is suitable only for smear microscopy from sputum. The sensitivity of this method was estimated to be 80% with a specificity of 96.7% (Famia et al. 2002). The utility of this method for culture was not evaluated.
Another recently published method describes the use of phenol and ammonium sulfate combination (PhAS) for liquefaction of sputum. But this needs an overnight precipitation and is not suitable for culturing as it kills the mycobacteria. The sensitivity and specificity of the method were 85% and 97% respectively (Selvakumar et al. 2002).
The sensitivity of the direct method of smearing (by ZN and/or auramine staining) has ranged from 40 to 85% in different laboratory settings. Involvement of a concentration step increased the sensitivity of the direct smear method from 54-57% to 63-80% (Bruchfeld et al. 2000; Garay et al. 2000). The CDC method has shown sensitivity levels around 66 to 83% (Scott et al. 2002, Pamia et al. 2002, our study). Inclusion of a centrifugation step (4000 g for 15 min) was reported to detect ≧5000 and 500 organisms/ml by smear microscopy and culture, respectively (Perera et al. 1999). However other reports have suggested that the overall diagnostic sensitivity of smear microscopy was not increased by sputum liquefaction and concentration (Wilkinson et al. 1997). It has been proposed that the use of fluorescence microscopy greatly improves the diagnostic value of the sputum smear especially in samples with a low density of bacilli that are likely to be missed on ZN stained smears (Githui et al. 1993). However the fluorescence microscopy technique would increase the cost.
Automated and semi-automated systems of cultivation of Mycobacteria currently available—The most sensitive though time consuming method for diagnosis of TB is culturing of the etiologic agent. There are several liquid and solid media available in the international market for this purpose. Since growth in a medium like Lowenstein-Jensen or other solid media takes at least 4-6 weeks to arrive at conclusive result, liquid culture systems employing radioactive materials or oxygen quenching and redox reagents have been introduced for their superior speed and sensitivity. They all require respiratory specimens that have been decontaminated by treatment with NALC—NaOR. The most widely used M.tb culturing kits based on these principles are: 1). BACTEC 460 TB (Becton Dickinson Diagnostic Instruments, Sparks, Md.). It detects consumption of CO2 radiometrically., 2). MB/BacT (Organon Teknika Corporation. Durham N.C.). It detects consumption of CO2 colorimetrically. 3). ESP Myco System (Difco Laboratories, Detroit, Mich.). It detects change in gas pressure. 4).
Mycobacterial Growth Indicator Tube (MGIr): Becton Dickinson, Cockeysville, Md.). It detects consumption of oxygen through an oxygen-sensitive fluorescent sensor. 5). Septi-Check APB System (BBL): Colonies are detected visually. All of the above systems use liquid culture media, (except for Septi-Check which is a biphasic system having both liquid and solid media) growth supplements, antibiotics and involve sophisticated technology. Except for the SeptiCheck AFB systems the other systems are automated and results can be obtained as early as 5-7 days. But with these automated systems it is not possible to study the colony morphology of the cells growing and the absence of contaminants have to be confirmed by performing AFB staining of the positive cultures. These systems are very costly and out of place in disease-endemic countries like India. The radiometric detection is a biological hazard too.
DNA isolation and in-house PCR—Numerous in-house methods aimed at isolation of PCR amplifiable M. tuberculosis DNA have been described. But a single method that is (a) compatible with smear microscopy, culture and DNA isolation from clinical samples and (b) suitable for all kinds of pulmonary and extrapulmonary samples, is not available. All the methods require pretreatment of the sputum samples with a mucolytic agent (NALC or DTT) and/or decontamination using NaOH and invariably involve the use of organic solvents and enzymes. The most popular methods for DNA isolation to the best of our knowledge are as follows:
Commercial Nucleic Acid Detection Kits.
In the international market, the following diagnostic kits employing amplification of the DNA or RNA of M. tuberculosis are available to the best of our knowledge. They use different amplification technologies (PCR, TMA, SDA and LCR). They require either respiratory or non-respiratory specimens that have been pretreated by NALC—NaOH (CDC method).
In summary, these commercial technologies are very sophisticated, costly including high running costs and generally not suitable for developing countries. These countries also happen to be high-incidence countries with maximum demand for cheap, rapid and efficient diagnostic test(s).
The two most commonly employed method of smear microscopy are direct smear examination (Akhtar et al., 2000) and concentration method recommended by CDC, Atlanta, U.S.A. (Koneman et al. 1997). Their drawbacks are enumerated below.
Drawbacks of Direct Smear Examination:
Drawbacks of Concentration Method (NALC—NaOH Method) of Smear Examination:
Clinical specimens are routinely decontaminated to remove organisms other than mycobacteria prior to culture on solid and liquid media. This is necessary as mycobacteria are slow-growing and cultures are swamped by faster-growing contaminating organisms. The most popularly used method of decontamination uses 2-3% NaOH along with the mucolytic agent NALC.
The main object of the present invention is to develop an effective and economical method of processing clinical samples useful for simple, rapid, safe, sensitive, and accurate diagnosis of tuberculosis and certain gram positive bacterial infections.
Another main object of the present invention is to develop the processed sample in the form of smear, culture, or Polymerase chain reaction (PCR) starting material, using PCR amplifiable mycobacterial DNA, and RNA.
Yet another object of the present invention is to develop a set of primers for screening patients for tuberculosis.
Still another object of the present invention is to develop a processed clinical sample.
Still another object of the present invention, to develop a single methodology, which alone encompasses sample processing and the most common laboratory methods of TB diagnostics, namely, smear microscopy, culture and PCR/RT-PCR.
Still another object of the present invention to develop a methodology that is applicable on all types of clinical material in pulmonary and extra pulmonary cases of tuberculosis and not just sputum.
Still another object of the present invention is to enable the microscopic visualization of AFB from samples with minimal bacterial load.
Still another object of the present invention is to develop a decontamination process other than NaOH treatment, which can avoid the cumbersome process of neutralization of samples and also reduce toxicity.
Still another object is to eliminate the use of NALC or other costly mucolytic agents.
Still another object of the present invention is to have a broad-based utility in the detection, isolation and amplification of DNA from all mycobacterial species including opportunistic pathogens.
Still another object of the present invention is to isolate inhibitor-free PCR amplifiable DNA from a wide-variety of pulmonary and extra-pulmonary samples.
Still another object of the present invention is to -avoid the use of organic solvents and enzymes for the isolation of clean PCR-amplifiable DNA from clinical material.
Still another object of the present invention is to use chaotropic agents in the process that is not toxic and is environment friendly and safe to use.
Still another object of the present invention is to develop a simple methodology that has minimal dependence on refrigerated high-speed centrifuge and sophisticated instrumentation.
Still another object of the present invention is to develop a method that involves minimal transfer of sample from one tube to another during processing, thus preventing loss of sample.
Still another object of the present invention is to develop a method that is compatible with isolation of mycobacterial RNA from clinical specimens.
Still another object of the present invention is to develop a modular technology for the diagnosis of tuberculosis.
Still another object of the present invention is to develop a highly sensitive method for processing clinical samples to identify the disease condition.
An effective and economical method of processing clinical samples useful for simple, rapid, safe, sensitive, and accurate diagnosis of bacterial infections using a composition comprising solution 1 consisting of Universal Sample Processing (USP) solution (composed of Guanidinium Hydrochloride ((GuHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3 -0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M), Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional can be replaced with sterile water), and Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and Solution C comprising Tween 20 of concentration ranging between 0.20.4% for isolating DNA (optionally, Solution 3 alone or Solution 3 with 0.03-0.1% Triton X 100 can be used for isolating DNA from high bacillary load or low junk containing samples in place of solutions A, B and C), said method comprising steps of adding 1.5 to 2 volumes of solution 1 to the sample and homogenization, adding Solution 2 or sterile water to the homogenate and centrifuging to obtain pellet, rewashing the pellet with solution 1 (optionally, for high junk containing samples), washing the solution 1-washed pellet with water, and resuspending the water-washed pellet in solution 3 to obtain processed sample in a from suitable for implementation of standard diagnostic procedures by smear microscopy, culture and PCR, and a Kit comprising above-stated solutions, and two sets of primers with first set devRf2 and devRr2 of SEQ ID No. 1 and SEQ ID No. 2 respectively and second set of primers devRf3, and devRr3 of SEQ ID No. 3, and SEQ ID No. 4 respectively, and a method of using said primers for screening patients of tuberculosis by polymerase chain reaction.
Accordingly, the present invention relates to an effective and economical method of processing clinical samples useful for simple, rapid, safe, sensitive, and accurate diagnosis of bacterial infections using a composition comprising solution 1 consisting of Universal Sample Processing (USP) solution (composed of Guanidinium Hydrochloride (GuHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3 -0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M), Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional can be replaced with sterile water), and Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and Solution C comprising Tween 20 of concentration ranging between 0.2-0.4% for isolating DNA (optionally, Solution 3 alone or Solution 3 with 0.03-0.1% Triton X 100 can be used for isolating DNA from high bacillary load or low junk containing samples in place of solutions A, B and C), said method comprising steps of adding 1.5 to 2 volumes of solution 1 to the sample and homogenization, adding Solution 2 or sterile water to the homogenate and centrifuging to obtain pellet, rewashing the pellet with solution 1 (optionally, for high junk containing samples), washing the solution 1-washed pellet with water, and resuspending the water-washed pellet in solution 3 to obtain processed sample in a from suitable for implementation of standard diagnostic procedures by smear microscopy, culture and PCR, and a Kit comprising above-stated solutions, and two sets of primers with first set devRf2 and devRr2 of SEQ ID No. 1 and SEQ ID No. 2 respectively and second set of primers devRf3, and devRr3 of SEQ ID No. 3, and SEQ ID No. 4 respectively, and a method of using said primers for screening patients of tuberculosis by polymetase chain reaction.
In an embodiment of the present invention, wherein an effective and economical method of processing clinical samples useful for simple, rapid, safe, sensitive, and accurate diagnosis of bacterial infections using a composition comprising solution 1 consisting of Universal Sample Processing (USP) solution (composed of Guanidinium Hydrochloride (GuHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%,, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M), Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional can be replaced with sterile water), and Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and Solution C comprising Tween 20 of concentration ranging between 0.2-0.4% for isolating DNA (optionally, Solution 3 alone or Solution 3 with 0.03-0.1% Triton X 100 can be used for isolating DNA from high bacillary load or low junk containing samples in place of solutions A, B and C), said method comprising steps of:
In yet another embodiment of the present invention, wherein the processed sample can be used in the form of smear, culture, or Polymerase chain reaction (PCR) starting material, using PCR amplifiable mycobacterial DNA, and RNA.
In still another embodiment of the present invention, wherein said Universal Sample Plrocessing (USP) solution comprises Guanidinium Hydrochloride (GUHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M.
In still another embodiment of the present invention, wherein the processed sample can be used for smear microscopy of mycobacteria including M. tuberculosis and Gram-positive organisms like Staphylococcus sp.
In still another embodiment of the present invention, wherein homogenizing is required for time duration ranging between 20-120 seconds.
In still another embodiment of the present invention, wherein Guanidinium hydrochloride of solution 1 lyses eukaryotic and Gram negative cells, denatures proteins, liquefies sample, and inactivates endogenous enzymes.
In still another embodiment of the present invention, wherein the processing is completed in a total time duration ranging between 1-2 hours.
In still another embodiment of the present invention, wherein said method yields inhibitor-free mycobacterial DNA for PCR based diagnostics of mycobacterial diseases including TB and leprosy.
In still another embodiment of the present invention, wherein Universal Sample Processing (USP) solution for processing only culture and smear samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 4M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.1M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing culture, smear, and PCR samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 5M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.2 M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing only smear, and PCR samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.2 M.
In still another embodiment of the present invention, wherein Guanidinium Hydrochloride (GuHCl), Sarcosyl, and beta-mercaptoethanol of USP act in a synergistic manner to perform optimal processing of all kinds of clinical samples.
In still another embodiment of the present invention, wherein presence of beta-mercaptoethanol and Sarcosyl is suitable for optimal processing of mucoid sputum samples.
In still another embodiment of the present invention, wherein presence of GuHCl is absolutely necessary along with beta-mercaptoethanol and Sarcosyl for optimal processing of mucopurulent sputum samples.
In still another embodiment of the present invention, wherein GuHCl acts as the principal inhibitor removal component in case of specimens containing blood, by denaturing hemoglobin and removing it from the specimen.
In still another embodiment of the present invention, wherein GuHCl acts as the principal decontaminating agent of clinical specimens.
In still another embodiment of the present invention, wherein PCR-amplifiable mycobacterial DNA can be obtained through simple lysis by boiling in presence of Solution 3 and/or 0.03-0.1% Triton X 100, without using Solution A, B and C in case of high bacillary load and/or lesser amount of junk containing samples.
In still another embodiment of the present invention, wherein the processed sample is free of contaminating organisms, proteins, enzymes, and interfering substances.
In still another embodiment of the present invention, wherein said method under smear microscopy can detect about 300-400 bacilli/ml of the sample. The sensitivity (limit of detection) can be further increased by using more than the prescribed 10% of the processed specimen for smear microscopy.
In still another embodiment of the present invention, wherein said method has about 30 folds enhancement in sensitivity over the conventional direct smear microscopy method.
In still another embodiment of the present invention, wherein said method in a smear shows sensitivity ranging between 97-99%.
In still another embodiment of the present invention, wherein said method in a smear shows specificity ranging between 83-92%.
In still another embodiment of the present invention, wherein said method shows enhancement in sensitivity by about 18% over CDC method of smear microscopy.
In still another embodiment of the present invention, wherein said method shows enhancement in sensitivity by about 30% over direct smear method.
In still another embodiment of the present invention, wherein said method in smears shows positive predictive value ranging between 80-96%.
In still another embodiment of the present invention, wherein said method in smears shows negative predictive value ranging between 91-99%.
In still another embodiment of the present invention, wherein said method in smears shows diagnostic accuracy of about 91%.
In still another embodiment of the present invention, wherein said method reduces counterstaining background enabling better viewing of the slides, enhances the gradation of slides, and reduces time and labour for reading a slide.
In still another embodiment of the present invention, wherein said method carries out decontamination of samples more efficiently as compared to the CDC method.
In still another embodiment of the present invention, wherein said method in a culture leads to a lag of about 1 week in appearance of the microbial colonies as compared to untreated microbes.
In still another embodiment of the present invention, wherein said method in a culture shows sensitivity of about 50%.
In still another embodiment of the present invention, wherein said method is more suitable than CDC method in tropical areas.
In still another embodiment of the present invention, wherein said method in culture runs at a neutral pH.
In still another embodiment of the present invention, wherein said method in PCR shows no inhibition of PCR assay with any type of clinical specimens tested.
In still another embodiment of the present invention, wherein said method in PCR shows sensitivity ranging between 96.5-100%.
In still another embodiment of the present invention, wherein said method in PCR shows specificity ranging between 71-100%.
In still another embodiment of the present invention, wherein said method in PCR using said primers shows positive predictive value ranging between 82-100%.
In still another embodiment of the present invention, wherein said method in PCR using said primers shows negative predictive value of 94-100%.
In still another embodiment of the present invention, wherein said method in PCR using said primers shows diagnostic accuracy of about 90%-100%.
In still another embodiment of the present invention, wherein samples can be obtained from sources comprising all types of sputum and other body fluids comprising FNAC, pus, pleural fluid, pericardial fluid, joint aspirate, peritoneal fluids, cerebrospinal fluids, endometrial aspirate, synovial fluid, gastric aspirate, endotracheal aspirate, urine, transtracheal aspirate, bronchoalveolar lavage, laryngeal swab and nasopharyngeal swab; body tissues comprising blood, pleural tissue, bone marrow and biopsy; solid organs comprising lymph node, bone, skin, and bovine samples comprising lymph gland, milk, and blood.
In still another embodiment of the present invention, wherein samples stored at about −20° C. for upto 2 months can be processed for PCR, smear-microscopy and culture.
In still another embodiment of the present invention, wherein said method in PCR can be used for both DNA, and RNA.
In still another embodiment of the present invention, wherein said composition shows mucolytic, decontaminating, protein denaturant, chaotropic, liquefying, tissue softening/digesting, and mycobacteria-releasing action.
In still another embodiment of the present invention, wherein said method in smear enables more bacilli to be smeared on the slide thereby increases both sensitivity, and efficiency.
In still another embodiment of the present invention, wherein said method in smear generally converts slides that are graded as 1+ or scanty by the direct method to 2+/3+ or 2+/1+ respectively.
In still another embodiment of the present invention, wherein said method helps process sputum samples lacking purulence or containing nasopharyngeal discharge and/or saliva and/or blood.
In still another embodiment of the present invention, wherein said method yields high quality smears from tissue biopsy samples suitable for very sensitive AFB smear microscopy.
In another main embodiment of the present invention, wherein an effective and economical method of processing clinical samples useful for simple, rapid, safe, sensitive, and accurate diagnosis of Mycobacterial infections especially tuberculosis caused by Mycobacterium tuberculosis using a composition comprising solution 1 consisting of Universal Sample Processing (USP) solution (composed of Guanidinium Hydrochloride (GuHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M), Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional can be replaced with sterile water), and Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and Solution C comprising Tween 20 of concentration ranging between 0.2-0.4% for isolating DNA (optionally, Solution 3 alone or Solution 3 with 0.03-0.1% Triton X 100 can be used for isolating DNA from high bacillary load or low junk containing samples in place of solutions A, B and C), said method comprising steps of:
In still another embodiment of the present invention, wherein the processed sample can be used in the form of smear, culture, or Polymerase chain reaction (PCR) starting material using PCR amplifiable mycobacterial DNA, and RNA.
In still another embodiment of the present invention, wherein the processed sample can be used in the form of smear, culture, or Polymerase chain reaction (PCR) starting material, wherein the PCR amplifiable mycobacterial DNA can be obtained by simple lysis by boiling.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution comprises Guanidinium Hydrochloride (GuHCl) of concentration ranging between 3-6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M.
In still another embodiment of the present invention, wherein the method maintains viability of mycobacteria and certain gram positive bacteria
In still another embodiment of the present invention, wherein homogenizing is required for time duration ranging between 20-120 seconds.
In still another embodiment of the present invention, wherein Guanidinium hydrochloride of solution 1 lyses eukaryotic and Gram negative cells, denatures proteins, liquefies sample, and inactivates endogenous enzymes.
In still another embodiment of the present invention, wherein the processing is completed in a total time duration ranging between 1-2 hours.
In still another embodiment of the present invention, wherein said method yields inhibitor-free mycobacterial DNA for PCR based diagnostics.
In still another embodiment of the present invention, wherein Universal Sample Processing (USP) solution for processing culture and smear samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 4M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.1M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing culture, smear, and PCR samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 5M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.2 M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing smear, and PCR samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.2 M.
In still another embodiment of the present invention, wherein the composition comprises solution 1 consisting of Universal Sample Processing (USP) solution, Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8, Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and optionally, Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, and/or Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and/or Tween 20 of concentration ranging between 0.2-0.4%
In still another embodiment of the present invention, wherein the use of Solution A, Solution B, and Solution C can be restricted to isolating DNA from low bacillary load samples only.
In still another embodiment of the present invention, wherein Guanidinium Hydrochloride (GuHCl), Sarcosyl, and beta-mercaptoethanol of USP act in a synergistic manner to perform optimal processing of all kinds of clinical samples.
In still another embodiment of the present invention, wherein presence of beta-mercaptoethanol and Sarcosyl is suitable for optimal processing of mucoid sputum samples.
In still another embodiment of the present invention, wherein presence of GuHCl is absolutely necessary along with beta-mercaptoethanol and Sarcosyl for optimal processing of mucopurulent sputum samples.
In still another embodiment of the present invention, wherein GuHCl acts as the principal inhibitor removal component in case of specimens containing blood, by denaturing hemoglobin and removing it from the specimen.
In still another embodiment of the present invention, wherein GuHCl acts as the principal decontaminating agent of clinical specimens.
In still another embodiment of the present invention, wherein PCR-amplifiable mycobacterial DNA can be obtained through simple lysis by boiling in presence of Solution 3 or by adding 0.03-0.1% Triton X 100 without using Solution A, B and C in case of high bacillary load and/or lesser amount of junk containing samples.
In still another embodiment of the present invention, wherein the processed sample is free of contaminating organisms, proteins, enzymes, and interfering substances.
In still another embodiment of the present invention, wherein said method under smear microscopy can detect 300-400 bacilli/ml of the sample. The sensitivity (limit of detection) can be further increased by using more than the prescribed 10% of the processed specimen for smear microscopy.
In still another embodiment of the present invention, wherein said method has about 30 folds enhancement in sensitivity over the conventional direct smear microscopy method.
In still another embodiment of the present invention, wherein said method in a smear shows sensitivity ranging between 97-99%.
In still another embodiment of the present invention, wherein said method in a smear shows specificity ranging between 83-92%.
In still another embodiment of the present invention, wherein said method shows enhancement in sensitivity by about 18% over CDC method of smear microscopy.
In still another embodiment of the present invention, wherein said method shows enhancement in sensitivity by about 30% over direct smear method.
In still another embodiment of the present invention, wherein said method in smears shows positive predictive value ranging between 80-96%.
In still another embodiment of the present invention, wherein said method in smears shows negative predictive value ranging between 91-99%.
In still another embodiment of the present invention, wherein said method in smears shows diagnostic accuracy of about 91%.
In still another embodiment of the present invention, wherein said method reduces counterstaining background enabling better viewing of the slides, enhances the gradation of slides, and reduces time and labour for reading a slide.
In still another embodiment of the present invention, wherein said method carries out decontamination of samples more efficiently as compared to the CDC method.
In still another embodiment of the present invention, wherein said method in a culture shows twice the viability of the microbes as compared to CDC method.
In still another embodiment of the present invention, wherein said method in a culture leads to a lag of about 1 week in appearance of the microbial colonies as compared to untreated microbes.
In still another embodiment of the present invention, wherein said method in a culture shows sensitivity of about 50%.
In still another embodiment of the present invention, wherein said method is more suitable in than for CDC method in tropical areas.
In still another embodiment of the present invention, wherein said method in culture runs at a neutral pH.
In still another embodiment of the present invention, wherein said method in PCR shows no inhibition of PCR assay with any type of specimen tested.
In still another embodiment of the present invention, wherein said method in PCR shows two sets of primers namely, devRf2 & devRr2, and devRf3, & devRr3 of gene devR of microbe Mycobacterium tuberculosis.
In still another embodiment of the present invention, wherein the primers devRf2, and devRr2 amplify a 308bp fragment of gene devR of microbe Mycobacterium tuberculosis.
In still another embodiment of the present invention, wherein the primers devRf3, and devRr3 amplify a 164 bp fragment of gene devR of microbe Mycobacterium tuberculosis.
In still another embodiment of the present invention, wherein said method in PCR using primers devRf2 and devRr2 shows 24 folds increase in sensitivity as compared to devRf and devRr.
In still another embodiment of the present invention, wherein said method in PCR using primers devRf3 and devRr3 shows at least 10 folds increase in sensitivity as compared to primers devRf and devRr.
In still another embodiment of the present invention, wherein samples can be obtained from sources comprising all types of sputum and other body fluids comprising FNAC, pus, pleural fluid, pericardial fluid, joint aspirate, peritoneal fluids, cerebrospinal fluids, endometrial aspirate, synovial fluid, gastric aspirate, endotracheal aspirate, urine, transtracheal aspirate, bronchoalveolar lavage, laryngeal swab and nasopharyngeal swab; body tissues comprising blood, pleural tissue, bone marrow and biopsy; solid organs comprising lymph node, bone, skin, and bovine samples comprising lymph gland, milk, and blood.
In still another embodiment of the present invention, wherein samples stored at about −20° C. for upto 2 months can be processed for PCR, smear-microscopy and culture.
In still another embodiment of the present invention, wherein said method in PCR can be used for both DNA, and RNA.
In still another embodiment of the present invention, wherein said composition shows mucolytic, decontaminating, protein denaturant, chaotropic, liquefying, tissue softening/digesting, and mycobacteria-releasing action.
In still another embodiment of the present invention, wherein said method in smear enables more bacilli to be smeared on the slide thereby increasing the sensitivity and the efficiency.
In still another embodiment of the present invention, wherein said method in smear generally converts slides that are graded as 1+ or scanty by the direct method to 2+/3+ or 2+/1+ respectively.
In still another embodiment of the present invention, wherein samples lacking purulence or containing nasopharyngeal discharge or saliva can be processed.
In still another embodiment of the present invention, wherein said method yields high quality smears from tissue biopsy samples suitable suitable for very sensitive AFB smear microscopy.
In still another embodiment of the present invention, wherein the said method shows no adverse effect on the acid-fast properties, viability, and integrity of the Mycobacterium tuberculosis.
In still another embodiment of the present invention, wherein said method is suitable for diagnosis of pulmonary as well as extrapulmonary tuberculosis with equal efficacy.
In still another embodiment of the present invention, wherein said method is compatible with culturing mycobacterium from clinical specimens in both solid and liquid media.
In still another embodiment of the present invention, wherein the said method can detect samples as positive which have been detected negative by direct and CDC methods of smear microscopy.
In still another embodiment of the present invention, wherein a Kit useful in processing clinical samples for simple, rapid, safe, sensitive, and accurate diagnosis of microbial disease conditions, said kit comprising solution 1 consisting of Universal Sample Processing (USP) solution, Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional, can be replaced with sterile water), Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and Solution C consisting of Tween 20 of concentration ranging between 0.2-0.4%, optionally two sets of primers with devRf2 and devRr2 of SEQ ID No. 1 and SEQ ID No. 2 respectively, and primers devRf3, and devRr3 of SEQ ID No. 3, and SEQ ID No. 4 respectively.
In still another embodiment of the present invention, wherein said kit is useful in processing clinical samples for detecting bacterial infections.
In still another embodiment of the present invention, wherein said kit is useful in processing clinical samples for detecting tuberculosis.
In still another embodiment of the present invention, wherein Universal Sample Processing (USP) solution comprises Guanidinium Hydrochloride (GuHCl) of concentration ranging between 36M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.3 M.
In still another embodiment of the present invention, wherein Universal Sample Processing (USP) solution for processing culture and smear samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 4M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.1M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing culture, smear, and PCR samples comprises Guanidinium. Hydrochloride (GuHCl) of concentration of about 5M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration ranging between 0.1-0.2 M.
In still another embodiment of the present invention, wherein said Universal Sample Processing (USP) solution for processing smear, and PCR samples comprises Guanidinium Hydrochloride (GuHCl) of concentration of about 6M, Tris-Cl of concentration ranging between 40-60 mM of pH ranging between 7.3-7.7, EDTA of concentration ranging between 20-30 mM, Sarcosyl of concentration ranging between 0.3-0.8%, beta-mercaptoethanol of concentration of about 0.2 M.
In still another embodiment of the present invention, wherein the composition comprises solution 1 consisting of Universal Sample Processing (USP) solution, Solution 2 consisting of Sodium phosphate of concentration ranging between 65 to 70 mM of pH ranging between 6.7 to 6.8 (optional, can be replaced with sterile water), Solution 3 consisting of Tween 80 of concentration ranging between 0.03 to 0.08%, and optionally, Solution A comprising Chelex 100 suspension of concentration ranging between 8-12%, and/or Solution B consisting of Triton X-100 of concentration ranging between 0.02-0.04%, and/or Tween 20 of concentration ranging between 0.2-0.4%.
In still another embodiment of the present invention, wherein a set of primers devRf2 and devRr2 of SEQ ID No. 1 and SEQ ID No. 2 respectively.
In still another embodiment of the present invention, wherein a set of primers devRf3, and devRr3 of SEQ ID No. 3, and SEQ ID No. 4 respectively.
In still another embodiment of the present invention, wherein a method of using primers of SEQ ID NO. 1, and 2 or SEQ ID No. 3 and 4 of gene devR for screening patients of tuberculosis said method comprising steps of conducting Polymerase Chain Reaction (PCR) using M. tuberculosis DNA or RNA obtained from the processed sample of the subject, identifying the subjects suffering from tuberculosis.
In still another embodiment of the present invention, wherein a processed clinical sample is obtained by processing a clinical specimen using method as described herewith.
In still another embodiment of the present invention, wherein samples can be obtained from sources comprising all types of sputum and other body fluids comprising FNAC, pus, pleural fluid, pericardial fluid, joint aspirate, peritoneal fluids, cerebrospinal fluids, endometrial aspirate, synovial fluid, gastric aspirate, endotracheal aspirate, urine, transtracheal aspirate, bronchoalveolar lavage, laryngeal swab and nasopharyngeal swab; body tissues comprising blood, pleural tissue, bone marrow and biopsy; solid organs comprising lymph node, bone, skin, and bovine samples comprising lymph gland, milk, and blood.
In still another embodiment of the present invention, wherein The method comprises of the use of a novel Universal Sample Processing (USP) solution. Briefly, the USP method comprises of homogenization, liquefaction and decontamination steps carried out on the clinical specimen by treatment with USP solution, concentration of mycobacteria by centrifugation and washing steps. Guanidinium hydrochloride comprises the active component of USP together with a reducing agent and a detergent in a buffered solution. This chaotropic agent Guanidinium hydrochloride effectively lyses eukaryotic cells, most bacteria excluding mycobacteria, denatures proteins, including mucin and thereby liquefying sputum, and inactivates endogenous enzymes including DNases and RNases. Proteins, debris and other contaminants are removed in subsequent rapid washing steps. The unique cell wall properties of mycobacteria render it selectively resistant to the action of chaotropic agent and the resultant bacteria are suitable for use in smear microscopy, culture, PCR, RNA isolation and procedures utilizing DNA and RNA isolated thereupon. The entire sample processing procedure can be completed in 1½-2 hours. The procedure does not involve steps of phenol:chloroform extraction, protease digestion, or precipitation steps, making the procedure ideal for processing many samples at a time. The method is applicable on all kinds of body fluids, blood, tissue biopsies, biopsies from vertebra and other bone joints and CSF. It is also suitable for use in processing tissue biopsies and milk of bovine origin. For body fluids other than sputum, concentration by centrifugation followed by USP solution and water wash is adequate for smear nucroscopy, culture, nucleic acid isolation and amplification. Tissue specimens require homogenization in USP solution prior to proceeding for the processing in the normal way.
The processed sample is directly used for smear microscopy, culture on solid media (such as Lowenstein Jensen (LJ) slants or Middlebrook 7H10 agar medium) or liquid medium (such as Middlebrook 7H9 medium used in MGIT system) and DNA and RNA extraction for amplification of mycobacterial DNA and RNA.
The duration of the methodology is as follows: 1½-2 hours for sample processing, 1½-1 hour for smear microscopy and culture and 4 hours for nucleic acid amplification. [The USP Method described here, is expected to have extremely great utility in the rapid diagnosis of tuberculosis owing to its high sensitivity and applicability to all types of clinical material. This method will enable correct identifying of a sizeable population of infected individuals who escape detection by the less sensitive direct smear method and therefore this method has utility worldwide. The methodology described here is also compatible with culture and PCR/RT-PCR tests. To the best. of our knowledge, no methodology has been described or is available which employs a single technology for smear microscopy, culture and PCR/RT-PCR on all types of clinical material for laboratory diagnosis of tuberculosis.
Thus it is clear that the method described here will find use in any laboratory that requires highly sensitive smear microscopy of acid-fast bacilli, effective decontamination of clinical samples for culture and the isolation of mycobacterial and M. tuberculosis DNA for PCR amplification, from clinical specimens. Therefore it has potential for inclusion in TB/mycobacterial diagnostic kits. The claimed technology can be used in a variety of clinical settings and laboratories. It can be used for smear microscopy in laboratories having minimal equipment (a light microscope and an ambient temperature centrifuge that can go up to a speed of 3000 rpm). It can be used for smear microscopy and culture in laboratories having additional facilities for mycobacterial culture. Finally PCR and RT-PCR test can be applied in sophisticated laboratories.]
The invention is a universal sample processing and diagnostic methodology to be used on any type of clinical specimen for the laboratory diagnosis of tuberculosis in a variety of clinical settings. It is rapid, inexpensive and easy and is compatible with smear microscopy, culture, DNA-RNA isolation and PCR/RT-PCR. It serves the quadruple purpose of (i) providing an extremely sensitive procedure for smear microscopy for the detection of Mtuberculosis, (ii) preparing the sample for culture on solid or liquid laboratory media, (iii) isolating DNA template free of PCR inhibitors and (iv) providing sensitive and specific diagnostic assays based on PCR. The methods have been evaluated on clinical specimens. USP smear microscopy showed a sensitivity of 97-99% in comparison to 68.6% by direct smear and 80% by the CDC method. Using culture as gold standard, the ‘short target’ PCR assay yielded a sensitivity of 98.5% vs the ‘long target’ PCR assay that showed a sensitivity of 73.2%. In terms of usefulness for culture, the USP method was not significantly different from the conventional methods method; 50.1% and 53.6% positivity was noted for the USP and the conventional methods of culturing, respectively. Thus it is clear that the method described here will find use in any laboratory that handles M. tuberculosis and MOTT bacilli containing specimens and requires highly sensitive smear microscopy of acid-fast bacilli, effective decontamination of clinical samples for culture and the isolation of mycobacterial and M. tuberculosis DNA for PCR amplification, from clinical specimens. Therefore it has potential for inclusion in TB/mycobacterial diagnostic kits.
Table 1. Upgradation in smear status by USP smear microscopy over direct and CDC smear methods.
Table 2. Upgradation in smear status by USP smear microscopy over direct smear Method.
Table 3. Effect of USP solution on the viability of M. tuberculosis.
Table 4. Comparative sensitivity of devR PCR assays using different primer pairs and M. tuberculosis DNA.
a shows pellets obtained for PCR after processing of sputum specimen using USP solution of varying composition.
b shows IS6110 PCR amplification profile of M. tuberculosis DNA isolated from the sputum specimen processed by USP solution of varying composition.
a effect of USP solution on sputa of different physical characteristics.
b shows effect of USP solution on a representative tissue biopsy specimen.
Data set 1. Evaluation of USP smear vs. Direct smear methods.
Data set 2. Evaluation of USP method vs. CDC method of smear microscopy
Data set 3. Sensitivity and specificity of PCR using ‘long target’ and ‘short target’ devR PCR and comparison with IS6110 based PCR assay.
Data set 4. Smear microscopy and culture status of extrapulmonary specimens processed by the USP method.
Data set 5. Sensitivity and specificity values (%) for devR- short target PCR for the extrapulmonary samples.
Data set 6. Sensitivity and specificity values (%) for IS6110 PCR for the extrapulmonary samples.
A multipurpose method has been developed for laboratory diagnosis of tuberculosis by smear microscopy, culture and PCR. The method is eminently suitable for the preparation of smears for highly sensitive microscopy. It effectively decontaminates samples making them suitable for culture and yields good quality inhibitor-free mycobacterial DNA for use in PCR-based diagnostic assays. It also enables the isolation of good quality mycobacterial RNA from clinical specimens employing common RNA isolation procedures.
The method for processing of sputum samples for smear microscopy, culture and PCR is as follows:
Reagents:
For Isolation of DNA from Low Bacillary Load Samples, the Following Additional Reagents are Needed:
Note: For the isolation of DNA from smear positive samples and sterile fluids, solutions A, B and C are not required; amplifiable DNA may be isolated by directly heating the sample in Solution 3 at 90-100 ° C. for 30-40 minutes (or Solution 3 may be supplemented with Triton X-100 at a final concentration of 0.01% (see
Methodology:
Sample Processing:
Processing of Sputum:
Processing of Uncoagulated Blood (with EDTA):
Processing of Other Body Fluids (Except Sputum and Stored Pleural Fluid Samnples):
Processing of Pleural Fluid:
If the pleural fluid is processed within 1 hour after collection, then processing can be carried out as described in “Processing of other body fluids.” If the pleural effusion is stored for more than one hour the proteins separate out as a coagulum. Such a sample should be processed as follows:
Processing of Tissue Biopsy (Fresh and Paraffin-embedded):
Smear Microscopy, Culture and PCR Technique:
To the pellet obtained after wash with sterile water, add 500 μl of Solution 3 and resuspend the pellet thoroughly. It may be noted that the pellet has a tendency to sediment along the wall of the tube as well as at the bottom after centrifugation. Care is to be taken that the pellet is completely resuspended to yield a homogeneous solution. This is important to obtain optimal results. Transfer it to a 1.5 ml polypropylene vial. The material is now ready for the three tests namely, smear microscopy, culture and PCR.
A). Smear Examination (10% of the Re-suspension is Used)
Smear 50 μl on a clean glass slide in a minimum area possible. If there is any particulate matter in the smear, care should be taken to spread it out evenly with the inoculation loop while smearing. Area of smearing preferably should not exceed 1 cm×1 cm.
Simplified method of USP smear preparation: Add 2-3 volumes of USP solution to the sputum (specimen volume less than or equal to 2 ml) collected in McCartney bottles. Mix by hand for 30-60 seconds to homogenize well. Allow to stand for 5-10 minutes. For highly tenacious and purulent sputum specimens, add at least 3 volumes of Solution 1, hand mix and incubate at 37° C. for 20-30 min. Fill the bottle with sterile water up to ⅔rd level and mix by inversion. Centrifuge at 3,000 g for 20 minutes in an ambient temperature clinical centrifuge. Decant supernatant carefully taking care that the pellet is not dislodged/lost. Re-suspend the pellet in 2-5 ml of Solution 1 and centrifuge as before. Decant the supernatant carefully and wash the pellet with ˜10 ml sterile water. Decant the supernatant carefully, add ˜10 drops of solution 2 with a dropper/Pasteur pipet to the pellet and re-suspend it thoroughly with the help of an inoculation loop. Take care to include in the final re-suspension the pellet deposited along the wall of the bottle during centrifugation. Pour 34 drops of the re-suspension on a clean slide and spread using a continuous rotational movement with an inoculation loop to produce a smear of about 20 mm by 10 mm. Allow the smear to air-dry for about 30 to 45 min, fix over flame, stain for AFB using ZN stain and examine slide under oil-immersion lens using a light microscope.
B.) Culture (50% of the Resuspension is Used)
Inoculate 200 μl onto LJ media. Incubate at 37° C.
C.) DNA Isolation for PCR (40% of the Resuspension is Used)
For DNA isolation, pellet the ‘Solution 3 resuspension’ at 12,000-13,000 rpm in a microcentrifuge tube. To the pellet add approximately 5 times the volume of Solution A, and 1/10th volume of each of Solution B and Solution C, as that of Solution A. Mix well, avoiding frothing. Heat at 90° C. for 40 minutes. Centrifuge at room temperature at 12,000 rpm for 5-10 minutes. Perform PCR with supernatant. Be careful not to add any debris or resin from Solution A into the PCR reaction.
Note: If the pellet size is drastically reduced so that it is not visible at all or the pellet size is very minute or the sample is a high bacillary load sample, lysis may be carried out by heating the ‘Solution 3 resuspension’ directly or after adding Triton X-100 (at a final concentration of 0.01%) at 90-100° C. for 30-40 minutes. The supernatant may be used directly for PCR. The addition of the lysis reagents (namely, solutions A, B and C) may be avoided in such cases; but for best results, it is recommended that the lysis reagents (Solution A, B and C) be used for isolation of PCR-amplifiable DNA (See below for details).
D). RNA isolation: The pellet obtained can be subjected to mycobacterial RNA isolation by any standard RNA isolation procedures or kits.
PCR Technique
The PCR assay targets the devR gene (Rv3133c) of M. tuberculosis and is specific for the organisms of the M. tuberculosis complex [Singh et al., 1999]. In published studies from our laboratory, the primer pair devRf and devRr was used in the ‘long target’ PCR assay that amplified a 513 bp fragment from the devR gene [Singh et al., 1999; 2000]. In a multi-centric study under DBT-funded project entitled “Evaluation of PCR-based test for tuberculosis”, [duration February 1997-February 2000, with financial support till 28.2.1999], the same PCR assay was used with coded sputum samples. The results of smear microscopy and culture were correlated with PCR results. Sensitvity was 100% for culture positive (14/14 culture positive) and 94% for smear positive samples (17/18 smear positive samples) [Ref: DBT Report on the analysis of the results of evaluation of different indigenously developed PCR assays for the diagnosis of tuberculosis. DBT, Govt. of India].
It was reasoned that amplification of a smaller region of the devR gene was likely to provide equivalent/better sensitivity with paucibacillary and smear negative samples. With this idea in mind two new primer pairs were designed to amplify shorter fragments of the devR gene. The ‘short target’ PCR assays were assessed first on purified M. tuberculosis DNA and subsequently validated on clinical specimens. The ‘short target’ primer pairs are, (i) devRf2, 5′ TGGCAACGGCATTGAACTGT 3′ and devRr2, 5′ TAAGCAGGCCCAGTAGCGT 3′ and (ii) devRf3, 5′ ATCTGTTGTCCCGCATGCC 3′ and devRr3, 5′ GTCCAGC GCCCACATCITT 3′.
The Following PCR Parameters were Used:
‘Short target’ assay: initial denaturation at 94° C. for 10 minutes; 45 cycles of denaturation at 94° C. for 1 minute, annealing at 52° C. for 1 minute and extension at 72° C. for 30 seconds; and a final extension at 72° C. for 10 minutes.
‘Long target’ assay: Identical parameters as for the ‘short target’ assay except that annealing and extension were performed at 65° C. for 90 seconds.
IS6110 assay: Published primers suggested by Eisenach et al. (1990) were used with the PCR reaction parameters: initial denaturation at 94° C. for 10 minutes followed by 45 cycles of 94° C. for 1 min and 60° C. for 1 min. 30 seconds, followed by a final extension at 72° C. for 5 minutes.
Reaction Components:
*To be reconstituted in PCR-grade water to obtain 20 μM concentration.
Total reaction volume is 40 μl (30 μl cocktail and 10 μl template). Dispense 30 μl cocktail into sterile 0.2 ml or 0.5 ml vials in a DNA-free hood. Add 10 μl template in a designated area away from the PCR-setup area. Give the tubes a brief spin for 30 seconds and place them in the thermal cycler. After the completion of the reaction (parameters given above) electrophorese the amplification products in 1.5-2.3% agarose gel in 0.5× TBE buffer at 80 volts for 20-30 minutes. Visualize the product (load at least 35 μl) by ethidium bromide staining under UV light.
(A) Function of USP solution components: To check for the function of each component of the USP solution, the USP solution was selectively depleted of the individual components and samples processed with the resulting solutions. USP solution consists of five components:
It was apparent that the mucolytic, decontaminating, protein denaturing, chaotropic, liquefying, tissue softening/digesting, mycobacteria-releasing actions which are the principal functions of USP solution are performed by the first three components, namely GuHCl, Sarcosyl and β-mercaptoethanol. To establish this, these three components were selectively depleted from the USP solution individually or in combination keeping the Tris-HCl and EDTA composition unchanged. The selectively depleted solutions were assessed for their effect on specimen processing, smear microscopy, PCR and culture (
All the solutions were used for processing of the sputum specimen as described and smear microscopy, PCR and culture was performed. The experiment was repeated twice for samples with varying physical characteristics.
The Principal Observations were:
(B) Rapid liquefying activity of USP.
(C) Smear microscopy. Representative slide smears prepared by the USP, CDC and direct smear methods are shown in
a). Negative samples turn to positive in USP smear: The sensitivity of the direct smear method and the USP smear method were 68.6% and 98.2%, respectively, with a specificity of 92.6% and 91.4% respectively on the panel of 571 sputum sample (Data set 1). All samples diagnosed as smear positive by the direct method were also positive by the USP and CDC methods of smear microscopy. Out of the 325 direct smear-negative samples, the USP method detected 97 additional samples as smear positive, which were positive by culture also, thereby recording an enhancement in sensitivity of 29.6%. A representative example is shown in
The USP smear method was also simultaneously compared with CDC method of smear microscopy in 325 out of these 571 specimens (Data Set 2). 29 specimens, which were smear negative by the CDC method, were detected as positive by the USP method of smear microscopy all of which were also culture positive. Thus USP method also fared better than a popularly used concentration method of smear microscopy (CDC method) in terms of sensitivity of detection (97.1% and 80% sensitivity for USP and CDC smear microscopy, respectively).
Evaluation of USP Smear vs. Direct Smear Methods
Gold Standard: Culture (on UJ media),, Sample strength, n=571
Measure of Association at 95% Confidence Interval
USP Smear Microscopy (ZN)
Sensitivity: 98.2% (95.9%-99.3%)
Specificity: 91.4% (86.9%-94.4%)
PPV: 93.9% (90.7%-96.1%)
NPV: 97.4% (94.1%-98.9%)
Diagnostic accuracy: 90.5%
Direct Smear Microscopy (ZN)
Sensitivity: 68.6% (63.2%-73.5%)
Specificity: 92.6% (88.4%-95.4%)
PPV: 92.6% (88.4%-95.4%)
NPV: 68.3% (62.9%-73.3%)
Diagnostic accuracy: 78.3%
Evaluation of USP Method vs. CDC Method of Smear Microscopy (ZN)
Gold standard: Culture (on L J media)
Sample strength, n=325.
Measure of association at 95% confidence interval.
USP Smear Microscopy (ZN)
Sensitivity: 97.1% (92.9%-98.9%)
Specificity: 83.3% (76.2%-88.6%)
PPV: 86.4% (80.5%-90.8%)
NPV: 96.3% (91.1%-98.6%)
Diagnostic accuracy: 90.5%
CDC Smear Microscopy (ZN)
Sensitivity: 80.0% (73.0%-85.6%)
Specificity: 89.67% (83.5%-93.8%)
PPV: 89.47% (83.2%-93.7%)
NPV: 80.35% (73.5%-85.8%)
Diagnostic accuracy: 84.6%
b). UDgradation in smear status: The USP method enhances the gradation of slides making them easier to read in a shorter period of time. Slides graded as scanty, 1+ or 2+ by the direct method of smear microscopy generally turned into 1+, 2+ or 3+ by the USP method.
This trend was observed when the results of USP method of smear microscopy were compared with that of the direct method over the panel of 571 samples (Table 1). The upgradation in the smear status was also observed when the USP solution method was compared with the CDC method (Table 2). Representative slides are shown in
(Values in parenthesis indicate sample strength).
(Values in parenthesis indicate sample strength)
Thus the USP solution method of smearing showed a very high sensitivity in the range of 97-98%, much better than the other two methods that were tested, with a specificity range of 83-91%. USP smear microscopy showed a positivity of 97-98% in culture positive samples vs. 80% by CDC and 68.6% by direct smear methods thereby recording an enhancement in sensitivity of ˜30% and 18% over the direct and CDC method of smear microscopy respectively. The increase in the sensitivity was significant as the sensitivity values of all the three methods were completely non-verlapping at a 95% confidence interval. The USP smear method did not miss a single sample detected by either the direct smear method or the CDC method. The specificity of the USP smear microscopy method when compared to the direct smear method was 91.3% and that when compared to the CDC method was 83.3%. The two values were overlapping at a 95% confidence interval. (Data Sets 1 and 2).
(D) Culture.
(i) Lethality of USP solution on M. tuberculosis spiked in sputum. A logarithmic phase M. tuberculosis culture was serially diluted up to 10000-fold in duplicate. 10% of one set of dilutions was inoculated onto LJ slant, 100% of each dilution of the duplicate set was spiked into 1 ml of sputum aliquots obtained from a patient suffering from COPD. The spiked samples were processed by the USP method and 10% of the processed material was inoculated onto LJ slant. The viable count obtained in the case of untreated cells was 720/ml and that in case of the spiked USP solution treated cells was 400/ml (at 10000-fold dilution) after 8 weeks (Table 3;
However it was found that USP solution containing 4 M GuHCl (in place of 5 M GuHCl) was less harsh on mycobacteria. More viable bacterial colonies were obtained when M. tuberculosis was treated with USP containing 4 M GuHCl than with USP having 6M GuHCl); 142 cells/ml in case of cells treated with USP containing 4 M GuHCl and 72 cells/ml in case of cells treated with 6 M GuHCl (at 100 fold dilution) after 4 weeks. The untreated cells of the same dilution corresponded to 277 cells/ml. But it decontaminated clinical samples with equal efficiency. So where culturing of M. tuberculosis from clinical samples is of prime importance, USP solution containing 4M GuHCl will increase the chances of culture positivity.
(ii) Effect of USP on the growth rate of M. tuberculosis: It is well known that treatment with decontaminating reagents like NaOH slows down the growth rate of M. tuberculosis from clinical material. A similar observation was made with USP solution-treated bacteria also. USP-treated M. tuberculosis colonies appeared after a lag of at least 1-11/2 weeks compared to that of untreated cells on solid media (Middlebrook 7H10 or LJ). NALC—NaOH treated M. tuberculosis (CDC method) also grew somewhat faster (visible colonies were obtained at least 45 days earlier) than USP-treated bacteria on solid media although no appreciable differences were observed between the two treatments when bacteria were cultured in liquid system BD BACTEC™ MGIT™ 960 System (Becton Dickinson, USA) [see below].
From the above experiments it was estimated that after USP (containing 5 M GuHCl) solution treatment at 37° C. for 20 minutes, 56-62% of the M. tuberculosis cells were rendered non-viable. Thus, USP solution is not grossly lethal to the M. tuberculosis.
(ili). The growth rate of M. tuberculosis is equivalent after treatment with USP solution and NALC—NaOH in liquid medium (Mycobacterial growth indicator tubes): It is well known that treatment with decontaminating agents adversely affects the viability of mycobacteria and slows down their growth rate. The effect of USP solution treatment on the growth rate of M. tuberculosis was compared to that of NALC—NaOH treatment (CDC method). A logarithmic phase M. tuberculosis culture grown in Middlebrook 7H9 liquid medium (with ADC supplement and 0.05% Tween 80) was divided into three sets. One set was treated with USP solution and the other set was treated with NALC—NaOH followed by neutralization with phosphate buffer (CDC protocol). All three sets (i.e., USP solution-treated, NALC—NaOH-treated and untreated) were inoculated into MGIT tubes with PANTA reagent and OADC supplement (Becton Dickinson, USA), and monitored for positive signal using BD BACTEC™ MGIT™ 960 System. Untreated cells gave a positive signal for growth in two days whereas the USP solution-treated cells and NALC—NaOH-treated cells gave a positive signal for growth in five days. This experiment was repeated and the same results were obtained. This suggests that both USP and CDC methods slow down the growth of M. tuberculosis in liquid medium (MGM) to an equal extent.
(iv)Evaluation of USP culture In a clinical setting. The USP method of culturing was compared with other conventional methods of culturing (CDC method, modified Pettrof's method and Nassua‘s method) on a panel of 571 specimens.
Notable findings were
(a). Both the USP and the conventional methods showed comparable percent-positivity. The positivity of the conventional methods (53.6%) was not significantly higher than that of the USP method (50.1%) and they overlapped at 95% confidence interval.
(b). USP method proved to be very efficient in decontamination and showed lesser number of contaminated cultures (n=5) compared to the conventional methods (n=21) thereby proving it to be more suitable for tropical countries.
(c). USP solution being a neutral pH solution, samples decontaminated with USP solution did not require any neutralization prior to culturing.
Thus in terms of sensitivity, effect on viability and effect on growth rate, the USP method of culture is comparable to the currently accepted methods for the culturing of M. tuberculosis from clinical samples.
(E) PCR.
The PCR test was performed on >700 samples of pulmonary and extra-pulmornary origin. Samples were either fresh or frozen at −20° C. for up to 2 months. Mycobacterial DNA was isolated for this purpose by the USP method. Not a single sample has showed inhibition in the PCR assay indicating that the DNA isolated from the clinical samples by the USP method was free of inhibitors. The PCR assay targets the devR gene of M. tuberculosis (Rv 3133c) and is specific for the organisms of the M. tuberculosis complex [Singh et al., 1999]. In the published studies, the PCR primer pair devRf and devRr amplified a 513 bp fragment from the target gene [Singh et al., 1999; 2000]. It was reasoned that amplification of a smaller region of the same gene was likely to provide equivalent/better sensitivity with paucibacillary and smear negative samples. With this idea in mind two new sets of primers mapping within the devR gene were used in order to amplify shorter fragments of the devR gene. The new primer pairs used were (i) devRf2 and devRr2 that amplify a 308 bp fragment (
(i) Assay specificity. The specificity of the assays was assessed by the addition of DNA from various mycobacterial species into the PCR reaction. The devRf2 and devRr2 primers showed excellent specificity and a band of expected size (308 bp), was obtained only with DNA from organisms belonging to the M. tuberculosis complex and not with DNA from other mycobacterial species (FIG. 13). However, with devRf3 and devRr3 primers, amplification (164 bp product) was obtained with DNA from M. kansasii and M. xenopi species in addition to M. africanum, M. bovis and M. tuberculosis. Further, no amplification was obtained with DNA from M. microti, which belongs to the M. tuberculosis complex (
(ii) Assay sensitivity using purified DNA. Serial dilutions of pure M. tuberculosis DNA were used in PCR assays to assess the limit of detection of the amplified products by ethidium bromide (Table 4,
(iii)Evaluation of the ‘short target’ and ‘long target’ PCR assays in a clinical setting. The results of the PCR assays were compared with that of culture and smear microscopy, of 571 samples collected from suspected tuberculosis patients (n=571) coming to the DOTS Centre at New Delhi Tuberculosis Center, Sunderlal Jain Hospital, Delhi and Tuberculosis research center, Chennai. The assays used were:
The sensitivity of the ‘short target’ assay was 98.5%. This was substantially better than that of the ‘long target’ assay, whose sensitivity was 73.2%. For example, out of the 571 sputum samples analyzed, 322 sputum samples were positive by the USP smear method and also culture positive. Out of these culture positive and USP smear positive samples, long target assay failed to detect 65 samples, which were detected, by the short target assay confirming the latter assay to be a more sensitive one. Thus the introduction of the ‘short target’ primers increased the devR assay sensitivity by 25.3% using culture positivity, as gold standard (Data Set 3) and 2-10 folds when compared using serial dilutions of M. tuberculosis genomic DNA (Table 4). The overall diagnostic accuracy of the short-target assay was about 7% higher than that of the long-target assay (Data Set 3).
The enhanced sensitivity of the ‘short target’ assay was however accompanied by a lower specificity of 77% as compared to a specificity of 91.9% with the ‘long target’ assay (Data set 3). The apparent reduction in specificity of the ‘short target’ assay is likely a reflection of the enhanced sensitivity of the assay and is unlikely to be due to false positivity/lower specificity. This is supported by the following:
Data Set 3
Sensitivity and specificity of PCR using ‘long target’ and ‘short target’ devR PCR). Using culture positivity as gold standard; Sample strength, n=506 for devR-long PCR n=571 for devR-short PCR.
Measure of association at 95% confidence interval.
devR-long:
Sensitivity: 73.2% (67.4% -78.2%)
Specificity: 91.9% (87.4%-94.9%)
PPV: 91.3% (86.5%-94.5%)
NPV: 77.1% (69.1%-79.5%)
Diagnostic accuracy: 81.8%
devR-short:
Sensitivity: 98.5% (96.3%-99.4%)
Specificity: 77% (71.0%-82.0%)
PPV: 85.2% (81.8%-88.5%)
NPV: 97.4% (93.7%-99.0%)
Diagnostic accuracy: 89.32%
IS6110:
Sensitihvity: 99.1% (97.1%-99.8%)
Specificity: 71.2% (65% -76.7%)
PPV: 82.28% (78.1%-85.8%)
NPV: 98.3% (94.7%-99.6%)
Diagnostic accuracy: 87.22%
Extrapulmonary samples: The USP method was evaluated for its performance in the diagnosis of tuberculous pleural effusion and lymphadenopathy in a blinded study with coded pleural fluid, pleural tissue and lymph-node biopsy specimens collected from the patients coming to the OPD of Safdaijung Hospital, New Delhi, India. The samples comprised of 100 specimens from 88 patients including 77 specimens from TB patients and 23 specimens from control subjects with diseases like malignancy, arnoebiasis, CHF, Sarcoidosis and reactive lymphadenopathy (nonspecific inflammation). Inclusion criteria was based on any one or more of the following parameters:
a) Histopathology/Cytopathology consistent with TB (b) Positive smear for AFB (c) Culture positive for M. tb. (d) Clinical response to ATT. Specimens were processed by the USP method and smear microscopy, culture and PCR were performed. In this study a comparison between devR and IS6110 based PCR was carried out. devR PCR was carried out with the primer pairs devRf3/devRr3
M. tuberculosis DNA that was isolated from the specimens was of good quality and no PCR inhibition was noticeable. The USP method of smear microscopy detected eight specimens (6 pleural fluid and one each of lymph node and pleural tissue biopsy) (Data Set 4) as positive against three with the conventional method of smear microscopy. The sensitivity of USP smear microscopy was 10.4% in this group of extrapulmonary specimens whereas that of the conventional method was only 3.9%. Thus the USP methods enabled improved conclusive AFB smear microscopy from tissue biopsy specimens and other extrapulmonary specimens, compared .to routine diagnostic methods. Five specimens were positive by the USP method of culturing (sensitivity 6.5%)(Data Set 4). The devR PCR showed better sensitivity with pleural tissue and lymph node (100% and 75% respectively) specimens than IS6110, (75% and 50% respectively) probably due to absence of any copies of IS6110 in these strains (Data Sets 5 & 6). Thus the USP method of specimen processing and devR PCR served as a useful modality to arrive at the definitive diagnosis of tuberculous pleural effusion and lymphadenopathy.
Data Set 6:
Sensitivity and Specificity Values (%) for IS6110 PCR for the Extrapulmonary Samples.
The statistical analysis was done using SAS 8.0 software (SAS Institute Inc. Cary, N.C., USA.). Descriptive statistics, frequency distribution and percentage of the variables have been calculated. To evaluate diagnostic accuracy and reliability of the tests in comparison to gold standards, sensitivity, specificity, positive and negative predictive values and their confidence intervals at 95% have been calculated.
(F) Simplified protocol for lysis of mycobacteria present in the processed specimen prior to DNA isolation for PCR: After USP processing if the pellet size is drastically reduced so as to be nearly invisible or is very minute or if the sample is a high bacillary load sample, lysis may be carried out by heating the resuspension directly in solution 3 or after addition of Triton X-100 (final concentration of 0.01%) at 90-100° C. for 30-40 minutes. Three AFB positive sputum specimens were processed by the USP method. The processed pellet was resuspended into resuspension solution (solution 3) and divided into three equal aliquots for each specimen. One aliquot was pelleted and to the pellet was added lysis reagents (namely, solutions A, B and C), to the second aliquot was added Triton X-100 at a final concentration of 0.01% and the other was kept as such. All the three were boiled at 90° C. for 40 minutes and PCR was carried out with the supernatant. All the three lysates in each of the three specimens yielded amplicons with almost equal intensity when analysed by agarose gel electrophoresis (
(G) USP method is applicable to diagnosis of mycobacteriosis caused by Mycobacterium other than tuberculosis type (MOTT) as it does not have any detrimental effect on them: MOTIT bacilli were subjected to USP treatment for 10-15 minutes at room temperature, followed by a wash with sterile triple distilled water. Each species was then subjected to smear microscopy, culture on LJ slant and PCR to check for detrimental effect of USP solution, if any. The MOCT species used were: M. avium, M. fortuitum, M. gordonae, M. intracellulare, M. kansasi, M. scrofulacem, M. smegmatis, M. phlei, and M. vaccae. Organisms belonging to the M. tuberculosis complex like M. aficatium, M. bovis, M. bovis(BCG), M. microti were also included in the study. All the organisms retained their AFB status (
(H) Samples Processed by the USP Method are Compatible with Procedures for RNA Isolation from M. tuberculosis:
The USP method is also compatible with the isolation of high quality M. tuberculosis RNA from sputum specimens. For this purpose USP solution with the following composition was used.6M Guanidinium hydrochloride, 50 mM Tris-Ci, pH 7.5, 25 mM EDTA, 0.5% Sarcosyl and 0.2 M β-mercaptoethanol.
The sputum specimen used was graded 3+ by the direct method of smear microscopy. To the specimen (5-7 ml), was added 1.5 times the volume of USP solution and the mixture was homogenized. To it was added 10 ml of sterile DEPC water and mixed. It was then centrifuged at 5,000 rpm for 10 minutes at room temperature. The pellet was again washed with 2-3 ml of USP solution followed by a wash with sterile DEPC water. The final processed pellet can be used as a starting material for RNA isolation by an appropriate means. For example, using the Qiagen Rneasy Mini Kit as per manufacturer‘s protocol. The isolated M. tuberculosis RNA was subjected to reverse transcription reaction using Stratrascript reverse transcriptase (RT) enzyme (Stratagene, USA) to obtain cDNA. The cDNA was subjected to amplification using M. tuberculosis rRNA-(23S rRNA) and mRNA (Rv 3134c gene)-specific primers to detect the presence of M. tuberculosis RNA. RT negative controls in which the isolated RNA was subjected to reverse transcription reaction without the reverse transcriptase enzyme were also run for PCR to monitor for the presence of any contaminating genomic DNA in the preparation. Amplifications corresponding to both the ribosomal and messenger cDNAs were obtained (
(1) Effect of USP solution on the viability and staining property of Gram staining bacteria: The effect of USP solution on the viability and staining properties of Escherichia coli, Pseudomanas sp., and Staphylococcus sp., the three most common microflora present in sputum specimens, was evaluated. The bacteria were treated with solution 1 for 10-15 minutes at room temperature followed by centrifugation and washing of the pellet with sterile water as described. The pellets were then resuspended in solution 3 and 10% was smeared in glass slides (in duplicates) and 40% was inoculated in nutrient agar media and incubated at 37° C. to monitor for any growth. This was followed by Gram staining of the slides. Gram staining was also performed on the untreated bacteria.
It was observed that both Pseudomonas sp. and E. coli, were lysed by treatment with USP solution as evident from the total loss of morphology as seen by Gram staining (
So USP solution lyses and kills Gram negative bacteria but does not have any detrimental effect on Gram positive bacteria like Staphylococcus sp. and hence can also be used for diagnosis of staphylococcal disease.
8. Main Advantages of the Invention:
Advantages of USP Culture.
Advantages of USP Nucleic Acids Isolation Method.
Advantares of devR-based PCR.
Advantae of Modular Technology.
This technology is suitable for any kind of body fluid and tissue biopsy (except for fecal matter which has not been tested).The claimed technology can be used in a variety of clinical settings and laboratories. (1) It can be used for smear microscopy in laboratories having minimal equipment (only a vortex shaker, light microscope and ambient temperature centrifuge that can go up to a speed of 4000 rpm). (2) It can be used for smear microscopy and culture in laboratories having additional facilities for mycobacterial culture. (3) Finally PCR and RT-PCR tests can be applied in sophisticated laboratories.
The technology thus can be marketed-as the following to suit individual requirements:
Kit 1: Universal sample processing (TJSP) kit [containing 46 M GuHCl and 0.1-0.2 β-mercaptoethanol; see “Detailed description” for details].
Kit 2: USP, smear and culture kit.
Kit 3: USP, smear, culture and DNA isolation kit.
Kit 4: Complete kit (USP, smear, culture, DNA isolation and PCR).
Kit 5: As a part of mycobacterial RNA isolation kit from clinical specimens.
9. Various Significant Aspects of the Invention
Other Aspects Relating to USP Solution.
Aspects Relatingg to Smear Microscopy.
Aspects Relating to Culture.
Aspects Relating to DNA Isolation and devR-based PCR.
Aspects Relating to RNA Isolation and Amplification Techniques:
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| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/IB03/03211 | 7/29/2003 | WO | 1/27/2006 |
