Claims
- 1. A method of nucleic acid molecule delivery into a fertilized egg comprising:
providing a fertilized egg prior to its formation of a protective layer; providing a nucleic acid molecule; and combining the nucleic acid molecule and the fertilized egg under conditions effective to allow the nucleic acid molecule to be delivered into the egg.
- 2. The method according to claim 1, wherein the nucleic acid molecule is heterologous to the egg.
- 3. The method according to claim 1, wherein the nucleic acid molecule is homologous to the egg.
- 4. The method according to claim 1, wherein the nucleic acid molecule is in an expression vector.
- 5. The method according to claim 4, wherein the expression vector is a linear vector.
- 6. The method according to claim 4, wherein the expression vector is a circular vector.
- 7. The method according to claim 4, wherein the expression vector comprises a label.
- 8. The method according to claim 7, wherein the label is selected from the group consisting of a radio-active label, a fluorescent label, a chemiluminescent label, and a biotinylated label.
- 9. The method according to claim 1, wherein the nucleic acid molecule comprises a label.
- 10. The method according to claim 9, wherein the label is selected from the group consisting of a radio-active label, a fluorescent label, a chemiluminescent label, and a biotinylated label.
- 11. The method according to claim 1, wherein the egg is from a species selected from the group consisting of marine fish, freshwater fish, and crustaceans.
- 12. The method according to claim 11, wherein the egg is from a crustacean species.
- 13. The method according to claim 12, wherein the egg is a shrimp egg.
- 14. The method according to claim 1, wherein said combining comprises:
combining a transfection reagent with the nucleic acid molecule and the fertilized egg.
- 15. The method according to claim 14, wherein the transfection reagent is selected from the group consisting of a cationic lipid reagent, a liposomal cationic lipid reagent, a cationic non-liposomal lipid reagent, an activated dendrimer reagent, and a cationic polyethyleneimine reagent.
- 16. The method according to claim 15, wherein the transfection reagent is a cationic polyethyleneimine.
- 17. The method according to claim 15, wherein the transfection reagent is a linear cationic polyethyleneimine reagent.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Patent Application Ser. No. 60/471,611, filed May 19, 2003, which is hereby incorporated in its entirety.
Government Interests
[0002] The subject matter of this application was made with support from NIH NBRS Grant No. 5R25GM56930-05 and Sea Grant No. NA86RG0041 Project No. R/AQ-59, and NIH Grant No. 2T34GM07684-24. The U.S. Government may have certain rights.
Provisional Applications (1)
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Number |
Date |
Country |
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60471611 |
May 2003 |
US |