Claims
- 1. A diagnostic method for determining an electrophoretic property of lymphocytes comprising
- measuring in an automatic electrophoretic apparatus a predetermined electrophoretic property of untreated lymphocytes taken from the peripheral blood or lymph tissues of humans or animals being diagnosed to obtain a pattern, said peripheral blood or lymph tissues being suspended in a physiological solution having an ionic strength of 0.11 to 0.21 at a concentration of 0.5.times.10.sup.6 to 20.times.10.sup.6 lymphocytes per ml of said physiological solution, and
- comparing the thus-obtained pattern of the electrephoretic property with a pattern of the same electrophoretic property of corresponding normal lymphocytes.
- 2. The method of claim 1 wherein the ionic strength is between 0.13 to 0.17.
- 3. The method of claim 1 wherein a fraction of the number of the lymphocytes having an electrophoretic mobility within a predetermined range to the total number of lymphocytes is measured.
- 4. The method of claim 1 wherein an average electrophoretic mobility of the lymphocytes having an electrophoretic mobility within a predetermined range is measured.
- 5. Theaethod of claim 1 wherein electrophoretic mobility of a peak in the pattern is measured.
- 6. The method of claim 1 wherein the automatic electrophoretic apparatus is operated by reversing the electric field.
- 7. The method of claim 1 wherein the lymphocytes are from peripheral blood and the increase in the fraction of the number of lymphocytes having an electrophoretic mobility lower than a predetermined value to the total number of lymphocytes is measured.
- 8. The method of claim 1 wherein the lymph tissue is thymus and the lymphocytes are thymocytes.
- 9. The method of claim 8 wherein the increase in the fraction of the number of thymocytes having an electrophoretic mobility higher than a predetermined value to the total number of thymocytes is measured.
- 10. The method of claim 1 for diagnosing cancer.
Priority Claims (1)
| Number |
Date |
Country |
Kind |
| 56-107399 |
Jul 1981 |
JPX |
|
BACKGROUND OF THE INVENTION
This application is a continuation-in-part application of U.S. application Ser. No. 389,549, filed June 17, 1982, now abandoned.
This invention relates to a method for examining cells, particularly, lymphocytes, leukocytes or the like by electrophoresis.
In recent years, cell electrophoresis has begun to attract attention as a means for examining cellular immunity. One of the methods of cell electrophoresis was proposed by D. Sabolovic et al., "Cell Electrophoresis: Clinical Application and Methodology", INSERM Symposium No. 11 (1979). In this method, it necessarily takes a long time due to manual operation to obtain results, and the method is not favorable for examining a number of living cells. As another method, a laser-Doppler method was developed by D. Steiner et al., European Journal of Cancer, 15, 1275-1280 (1979), however, no satisfactorily good resolution of an electrophoretic pattern is obtained.
In these situations, a development of an apparatus and a method of electrophoresis of high performance enabling the rapid determination has been desired.
It is an object of the invention to provide a method for examining cells, particularly lymphocytes in peripheral blood or lymphatic tissues, comprising measuring an electrophoretic property of cells by an automatic electrophoretic apparatus and comparing the obtained pattern of the electrophoretic property of cells to be examined with the pattern of the electrophoretic property of normal host-cells.
US Referenced Citations (11)
Foreign Referenced Citations (1)
| Number |
Date |
Country |
| 2054839 |
Feb 1981 |
GBX |
Non-Patent Literature Citations (3)
| Entry |
| Abramson et al., The Electro Chemical Society Preprint, 71-12 (1937 meeting), pp. 115-131. |
| Zwergel et al., Microscopia Acta 80(1978) pp. 383-390. |
| Kyorin Abstract of Japanese Patent 56-1351. |
Continuation in Parts (1)
|
Number |
Date |
Country |
| Parent |
389549 |
Jun 1982 |
|
Patent Grant
4632743
