Claims
- 1. An E. coli cell wherein the genome comprises a dapD gene inactivated by mutation and which E. coli cell is transformed with a plasmid comprising a functional dapD gene.
- 2. The E. coli according to claim 1, wherein said mutation is a deletion mutation.
- 3. The E. coli according to claim 1, wherein said plasmid further comprises a "cer" sequence which maintains the plasmid in a monomeric state.
- 4. The E. coli cell of claim 1, wherein said plasmid further comprises a gene that encodes a heterologous protein operably linked to a promoter.
- 5. The E. coli according to claim 4, wherein said heterologous protein is hirudin, gamma-interferon, catechol 2,3 oxygenase or alpha-antitrypsin.
- 6. A process for preparing a heterologous protein which comprises the steps of:
- culturing the E. coli according to claim 4 in a DAP-free medium containing lysine under conditions such that said heterologous protein is produced; and
- isolating said protein from the culture.
Priority Claims (2)
Number |
Date |
Country |
Kind |
86 11311 |
Aug 1986 |
FRX |
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87 09935 |
Jul 1987 |
FRX |
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Parent Case Info
This is a continuation of application Ser. No. 07/081,447, filed Aug. 4, 1987, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4762791 |
Goeddel et al. |
Aug 1988 |
|
Foreign Referenced Citations (2)
Number |
Date |
Country |
0185512 |
Jun 1986 |
EPX |
8115385 |
Jul 1981 |
FRX |
Non-Patent Literature Citations (4)
Entry |
Harvey et al Proc. Natl Acad. Sci, USA vol. 83 pp. 1084-1088 (1986). |
Shevchenko et al, Chemical Abstracts vol. 100 Abstract No. 20447(f) 1984. |
Richaud et al J. Biol. Chem. vol. 259, p. 14824 (1984). |
Bollen et al. DNA vol. 2 pp. 255-264 (1983). |
Continuations (1)
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Number |
Date |
Country |
Parent |
81447 |
Aug 1987 |
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