Claims
- 1. A method of delivering a molecule to the skeletal muscle of a mammal in vivo comprising:
injecting a local anesthetic into the skeletal muscle of the mammal; injecting a molecule into an injection site in the skeletal muscle; positioning electrodes near the injection site such that current traveling through the electrodes passes through the injection site; and electrically stimulating the muscle with an electrical current having a field strength of from about 10 V/cm to about 300 V/cm.
- 2. The method of claim 1, wherein the electrical current has a field strength of from about 10 V/cm to about 233 V/cm.
- 3. The method of claim 2, wherein the electrical current is delivered as one or more square bipolar pulses.
- 4. The method of claim 1, wherein the electrical current has a field strength of from about 12 V/cm to about 175 V/cm.
- 5. The method of claim 1, wherein the electrical current has a field strength of from about 125 V/cm to about 233 V/cm.
- 6. The method of claim 1, wherein the electrical current is delivered as one or more square bipolar pulses.
- 7. The method of claim 1, wherein the local anesthetic is Marcain.
- 8. The method of claim 7, wherein the electrical current has a field strength of from about 10 V/cm to about 233 V/cm.
- 9. The method of claim 7, wherein the electrical current has a field strength of from about 12 V/cm to about 175 V/cm.
- 10. The method of claim 7, wherein the electrical current has a field strength of from about 125 V/cm to about 233 V/cm.
- 11. The method of claim 7, wherein the electrical current is delivered as one or more square bipolar pulses.
- 12. The method of claim 1, wherein the molecule is an antigen.
- 13. The method of claim 12, wherein the antigen is a protein.
- 14. The method of claim 1, wherein the molecule in a nucleic acid molecule.
- 15. The method of claim 14, wherein the nucleic acid molecule is a DNA molecule.
RELATED APPLICATIONS
[0001] This application is a continuation of copending U.S. patent application Ser. No. 09/565,140 of Iacob Mathiesen and Stig Tollefsen filed May 5, 2000 and entitled “Method for Genetic Immunization and Introduction of Molecules into Skeletal Muscle and Immune Cells,” which is related to and claims the benefit of U.S. Pat. No. 6,110,161 of Iacob Mathiesen and Terje Lømo filed Apr. 3, 1998 and entitled “Method for Introducing Pharmaceutical Drugs and Nucleic Acids Into Skeletal Muscle,” which is related to and claims the benefit of U.S. Provisional Application Ser. No. 60/042,594 of Iacob Mathiesen and Terje Lømo filed Apr. 3, 1997 and entitled “Apparatus and Method for Introducing Pharmaceutical Drugs and Genetic Material Into Skeletal Muscle.” These applications and this patent are hereby incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60042594 |
Apr 1997 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09565140 |
May 2000 |
US |
Child |
09899561 |
Jul 2001 |
US |