Claims
- 1. A method for influencing pollen development in transgenic plants by modifying the carbohydrate metabolism, comprising the following steps:
a) producing a recombinant nucleic acid molecule, comprising the following sequences:
regulatory sequences of a promoter that is active in anthers, in the tapetum and/or in pollen; operatively linked thereto a DNA sequence which codes for a protein having the enzymatic activity of a sucrose isomerase; and operatively linked thereto regulatory sequences which can serve as transcription, termination and/or polyadenylation signals in plant cells; b) transferring the nucleic acid molecule from a) to plant cells and c) regenerating transgenic plants.
- 2. The recombinant nucleic acid molecule, comprising
a) regulatory sequences of a promoter that is active in anthers, in the tapetum and/or in pollen; b) operatively linked thereto a DNA sequence which codes for a protein having the enzymatic activity of a sucrose isomerase; and c) operatively linked thereto regulatory sequences which can serve as transcription, termination and/or polyadenylation signals in plant cells.
- 3. The recombinant nucleic acid molecule according to claim 2, wherein the DNA sequence originates from Erwinia rhapontici.
- 4. The recombinant nucleic acid molecule according to claim 2, wherein the promoter is the T29 promoter.
- 5. A vector comprising a recombinant nucleic acid molecule according to claim 2.
- 6. A microorganism comprising a recombinant nucleic acid molecule according to any one of claims 2 to 4.
- 7. A microorganism comprising the vector of claim 5.
- 8. A method for generating male sterile plants comprising the transfer of a recombinant nucleic acid molecule according to any one of claims 2 to 4 to plant cells.
- 9. A method for generating male sterile plants comprising the transfer of the vector of claim 5 to plant cells.
- 10. A transgenic plant cell comprising a recombinant nucleic acid molecule according to any one of claims 2 to 4.
- 11. A transgenic plant cell comprising the vector of claim 5.
- 12. A transgenic plant, protoplast, callus, seed, tuber, cutting, or harvest product comprising the plant cell of claim 11.
- 13. A recombinant nucleic acid molecule comprising a DNA sequence from Erwinia rhapontici or a part thereof that codes for a protein having the enzymatic activity of a palatinase.
- 14. A recombinant nucleic acid molecule according to claim 13, wherein the DNA sequence is SEQ ID No. 1.
- 15. A recombinant nucleic acid molecule containing a DNA sequence from Erwinia rhapontici or a part thereof that codes for a protein having the enzymatic activity of a trehalulase.
- 16. The recombinant nucleic acid molecule according to claim 15, wherein the DNA sequence is SEQ ID No. 7.
- 17. A method for generating male fertile hybrid plants, comprising the following steps:
a) producing a first transgenic male sterile parent plant, comprising a nucleic acid molecule which codes for a protein having the enzymatic activity of a sucrose isomerase, b) producing a second transgenic parent plant, comprising a nucleic acid molecule which codes for a protein having the enzymatic activity of a palatinase and/or a protein having the enzymatic activity of a trehalulase, c) crossing the first parent plant with the second parent plant to generate a hybrid plant, wherein the hybrid plant is male fertile.
- 18. A method for generating male fertile hybrid plants comprising the following steps:
a) producing a first transgenic male sterile parent plant comprising a nucleic acid molecule which codes for a protein having the enzymatic activity of a sucrose isomerase, b) producing a second transgenic parent plant comprising a nucleic acid molecule which codes for a protein which has the biological activity of a sucrose isomerase inhibitor, c) crossing the first parent plant with the second parent plant for generating a hybrid plant, wherein the hybrid plant is male fertile.
- 19. A method for generating male fertile hybrid plants comprising the following steps:
a) producing a first transgenic male sterile parent plant comprising a nucleic acid molecule which codes for a protein having the enzymatic activity of a sucrose isomerase, b) producing a second transgenic parent plant comprising a nucleic acid molecule which codes for a ribozyme which is directed against sucrose isomerase mRNA, c) crossing the first parent plant with the second parent plant for generating a hybrid plant, wherein the hybrid plant is male fertile.
- 20. A method for generating male fertile hybrid plants, comprising the following steps:
a) producing a first transgenic male sterile parent plant comprising a nucleic acid molecule which codes for a protein having the enzymatic activity of a sucrose isomerase, b) producing a second transgenic parent plant comprising a nucleic acid molecule which codes for a sucrose isomerase antisense or sense RNA, c) crossing the first parent plant with the second parent plant to generate a hybrid plant, wherein the hybrid plant is male fertile.
- 21. The method of claim 17, wherein a glycosylation site is inactivated within the protein having the enzymatic activity of a palatinase and/or the protein having the enzymatic activity of a trehalulase due to at least one amino acid exhange in comparison with the wild type protein.
- 22. The recombinant nucleic acid molecule of claim 13, wherein a glycosylation site is inactivated within the protein having the enzymatic activity of a palatinase due to at least one amino acid exchange in comparison with the wild type protein.
Priority Claims (2)
Number |
Date |
Country |
Kind |
10045113.6 |
Sep 2000 |
DE |
|
10006413.2 |
Feb 2000 |
DE |
|
RELATED APPLICATIONS
[0001] This application is a continuation under 35 U.S.C. 111(a) of International Application No. PCT/EP01/01412 filed Feb. 9, 2001 and published in German on Aug. 16, 2001 as WO 01/59135 A1, which claimed priority from German Application No. 100 45 113.6 filed Sep. 13, 2000 and German Application No. 100 06 413.2 filed Feb. 14, 2000, which applications and publication are incorporated herein by reference.
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/EP01/01412 |
Feb 2001 |
US |
Child |
10223277 |
Aug 2002 |
US |