Claims
- 1. A process for obtaining a material possessing glucose tolerance factor activity, comprising:
- (i) contacting a eukaryotic cell mass with a mixture of water and an alcohol to form a water phase and an alcohol phase;
- (ii) separating the alcohol phase from the water phase and isolating a water phase extract; and
- (iii) subjecting said water phase extract to gel exclusion chromatography, eluting all material having a molecular weight of approximately 720 to 1120 to obtain a material possessing glucose tolerance factor activity and capable of binding with insulin.
- 2. The process of claim 1, comprising eluting all material having a molecular weight of approximately 820 to approximately 1020.
- 3. A process for obtaining a material possessing glucose tolerance factor activity, comprising:
- (i) contacting a eukaryotic cell mass with a mixture of water and an alcohol to form a water phase and an alcohol phase;
- (ii) separating the alcohol phase from the water phase and isolating a water phase extract;
- (iii) freeze drying said water phase extract; and
- (iv) subjecting said freeze dried water phase extract to gel exclusion chromatography, eluting all material having a molecular weight of approximately 720 to 1120 to obtain a material possessing glucose tolerance factor activity and capable of binding with insulin.
Parent Case Info
This is a division of application Ser. No. 07/409,546, filed on Sept. 19, 1989.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4985439 |
Hwang et al. |
Jan 1991 |
|
Non-Patent Literature Citations (2)
Entry |
"Preparation of Chromium-Containing Material of Glucose Tolerance Factor Activity from Brewer's Yeast Extracts and by Synthesis", J. Agric. Food Chem., vol. 25, No. 1, pp. 162-166, Toepfer et al., 1977. |
"Present Knowledge of the Role of Chromium", Federation Proceedings, vol. 33, No. 11, pp. 2275-2280, Mertz et al., Nov. 1974. |
Divisions (1)
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Number |
Date |
Country |
Parent |
409546 |
Sep 1989 |
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