Claims
- 1. A method for isolating DNA contained in a biological sample, comprising:
combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier; separating the DNA-bound carrier from other components; dissociating the bound DNA from the DNA-binding carrier; and recovering dissociated DNA.
- 2. The method of claim 1, wherein the DNA-containing biological sample comprises blood, a cell, or a biological tissue.
- 3. The method of claim 1, wherein the DNA-containing biological sample is not pretreated.
- 4. The method of claim 1, wherein the DNA-containing biological sample is not pretreated by centrifugation.
- 5. The method of claim 1, wherein the DNA-binding carrier is not hydroxyapatite.
- 6. The method of claim 1, wherein the method is conducted without phosphate.
Priority Claims (1)
Number |
Date |
Country |
Kind |
9/256795 |
Sep 1997 |
JP |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation application of U.S. application Ser. No. 09/508,119, filed Sep. 18, 1998, which is a National Stage Application of PCT/JP98/04201, filed Sep. 18, 1998, which was not published in English under PCT Article 21(2) (being published in Japanese), the disclosures of which are expressly incorporated by reference herein in their entireties. The present application claims priority to Japanese Application 9/256795, filed Sep. 22, 1997.
Continuations (1)
|
Number |
Date |
Country |
Parent |
09508119 |
Jun 2000 |
US |
Child |
09984742 |
Oct 2001 |
US |