Claims
- 1. A method for the determination of cells in a sample, said method comprising the steps of:
- (1) separating the cells from the sample,
- (2) washing the separated cells with:
- (a) an iron(III) chelate solution and
- (b) a non-ionic surfactant solution and
- (3) contacting the washed cells with a redox reagent so as to produce a detectable change due to the presence of the cells wherein said redox reagent comprises a cobalt(III) complex and a water soluble metalizable dye.
- 2. A method according to claim 1 further comprising a buffer wash after and/or before step 2).
- 3. A method according to claim 1 wherein said cells are separated from said sample by filtration.
- 4. A method according to claim 1 wherein said sample is urine.
- 5. A method according to claim 1 wherein said iron complex is Fe(III)ethylenedinitrilotetraacetic acid.
- 6. A method according to claim 1 wherein said surfactant is a octylphenoxypolyethoxy ethanol.
- 7. A method according to claim 1 wherein said redox reagent further comprises an electron transfer agent and a carbon source.
- 8. A method according to claim 1 wherein said cobalt(III) complex is cobalt (2,2'-bipyridine)bis(1,2-diaminoethane)cobalt(III) chloride and the water soluble metallizable dye is 2-[(5-carboxy-2-pyridyl)azo]-1-naphthol-4-sulfonic acid, diammonium salt.
CROSS REFERENCE TO RELATED APPLICATIONS
The present application is related to commonly assigned U.S. Ser. No. 890.051 filed July 28, 1986 by E. R. Schmittou entitled COBALT CONTAINING REAGENTS AND METHODS FOR THE DETERMINATION OF ANALYTES IN AQUEOUS FLUIDS.
US Referenced Citations (8)
Foreign Referenced Citations (1)
Number |
Date |
Country |
55-55757 |
Apr 1983 |
JPX |