Claims
- 1. A method for nucleotide sequencing, comprising:
continuously generating a reaction product in a nucleotide extension reaction of a polynucleotide chain; detecting the reaction product; and determining the alignment in the sequentially extending polynucleotide.
- 2. The method according to claim 1, wherein a DNA polymerase or an RNA polymerase is employed to facilitate the extension reaction of a polynucleotide chain.
- 3. The method according to claim 1, wherein the reaction product is a fluorescent substance.
- 4. The method according to claim 3, wherein a nucleotide as a starting substrate of the extension reaction of polynucleotide chain is a fusion substance of a fluorescent substance and a nucleobase; and wherein the reaction product is a fluorescent substance released from the fusion substance.
- 5. The method according to claim 4, wherein a DNA polymerase or an RNA polymerase catalyzes the release of the reaction product from the fusion substance.
- 6. The method according to clam 4, wherein the fusion substance is a deoxyribonucleotide 5′ triphosphate ester or a ribonucleotide 5′ triphosphate ester.
- 7. The method according to claim 3, wherein the excitation and emission profile of the fluorescent substance varies, depending on each nucleotide as the substrate of the extension reaction.
- 8. A method for comparing the gene sequences of plural biological species or different individuals, comprising:
determining the nucleotide sequences of two or more polynucleotide chains using the method according to claim 1; and comparing the nucleotide sequences to detect the differences between the nucleotide sequences.
- 9. A method for detecting a gene causing the difference in biological species or individuals, comprising employing the method according to claim 8.
- 10. A diagnostic method, comprising:
determining the nucleotide sequence of a test subject using the method according to claim 1;determining the nucleotide sequence of a polynucleotide chain from a patient in need thereof using the method according to claim 1; and comparing the nucleotide sequences to detect the differences between the nucleotide sequences.
- 11. The method of claim 10, wherein said test subject has a nucleotide sequence encoding a gene causing a disease selected from the group consisting of obesity and diabetes mellitus.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application claims priority to PCT International Patent Application No. PCT/JP01/07671, filed on Sep. 5, 2001, and Japanese Patent Application No. JP 2000-272489, filed on Sep. 8, 2000, which are hereby incorporated by reference in their entirety.
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/JP01/07671 |
Sep 2001 |
US |
Child |
10383556 |
Mar 2003 |
US |