Method for preparing a pharmaceutically active enantiomeric or enantiomerically enriched sulfoxide compound by enantioselective bioreduction of a racemate sulfoxide compound

Abstract

A compound of formula (II), either as a single enantiomer or in an enantiomerically enriched form ##STR1## wherein: ##STR2## and ##STR3## (wherein N in the benzimidazole moiety of Het.sub.2 means that one of the carbon atoms substituted by any one of R.sub.6 to R.sub.9 optionally may be exchanged for an unsubstituted nitrogen atom; R.sub.1, R.sub.2 and R.sub.3 are the same or different and selected from hydrogen, alkyl, alkoxy optionally substituted by fluorine, alkylthio, alkoxyalkoxy, dialkylamino, piperidino, morpholino, halogen, phenylalkyl, phenylakoxy; R.sub.4 and R.sub.4, are the same or different and selected from hydrogen, alkyl, aralkyl; R.sub.5 is hydrogen, halogen, trifluoromethyl, alkyl, alkoxy; R.sub.6 -R.sub.9 are the same or different and selected from hydrogen, alkyl, alkoxy, halogen, haloalkoxy, alkylcarbonyl, alkoxycarbonyl, oxazolyl, trifluoroalkyl or adjacent groups R.sub.6 -R.sub.9 may complete together with the carbon atoms to which they are attached optionally substituted ring structures; R.sub.10 is hydrogen or alkoxycarbonyloxymethyl; R.sub.11 is hydrogen or forms an alkylene chain together with R.sub.3 ; R.sub.12 and R.sub.13 are the same or different and selected from hydrogen, halogen or alkyl) is obtained by stereoselective bioreduction of a compound of formula (II) in racemic form.

Description

This application is a 371 of PCT/SE 95/01416 field Nov. 27, 1995,

The present invention relates to a method of obtaining compounds as defined below, either as a single enantiomer or in an enantiomerically enriched form.

BACKGROUND OF THE INVENTION

The racemic form of the compounds prepared by the method of the present invention are known compounds. Some of the compounds are also known in single enantiomeric form. The compounds are active H.sup.+ K.sup.+ ATPase inhibitors and they, including their pharmaceutically acceptable salts, are effective acid secretion inhibitors, and known for use as anti ulcer agents. The compounds, which include the known compounds omeprazole (compound of formula (IIa) below), Iansoprazole (compound of formula (IIc) below) and pantoprazole (compound of formula (IIb) below), are known for example from European Patent specifications EP 5129 and 124495, EP 174726 and EP 166287.

These compounds, being sulfoxides, have an asymmetric centre in the sulfur atom, i.e. exist as two optical isomers (enantiomers). It is desirable to obtain compounds with improved pharmacokinetic and metabolic properties which will give an improved therapeutic profile such as a lower degree of interindividual variation.

The separation of enantiomers of omeprazole in analytical scale is described in e.g. J. Chromatography, 532 (1990), 305-19. Also the separation of enantiomers of compounds with which the present invention is concerned, including omeprazole and pantoprazole, is described in German Patent Specification DE 4035455.

Recently there has been a great deal of literature published relating to the synthesis of optically active compounds using biocatalysts. The majority of this work has been aimed at finding routes to single enantiomer forms of pharmaceuticals. The reactions receiving most attention have been those involved in the preparation of esters, acids and alcohols due to the general utility of these funtionalities in synthesis and also because the biocatalysts are readily available.

Studies on the synthesis of optically active sulfoxides are relatively rare partly due to the small number of pharmaceuticals containing sulfoxide groups and partly due to the fact that enzymes that react with the sulphur centre are not available commercially. The enantioselective reduction of methylphenylsulfoxide to the sulfide has been discussed by Abo M., Tachibana M., Okubo A. and Yamazaki S. (1994) Biosci. Biotech. Biochem. 596-597.

DESCRIPTION OF THE INVENTION

According to the present invention there is provided a method of obtaining a compound of formula (II) either as a single enantiomer or in an enantiomerically enriched form: ##STR4## wherein: ##STR5## and ##STR6## wherein: N in the benzimidazole moiety of Het.sub.2 means that one of the carbon atoms substituted by any one of R.sub.6 to R.sub.9 optionally may be exchanged for an unsubstituted nitrogen atom;

R.sub.1, R.sub.2 and R.sub.3 are the same or different and selected from hydrogen, alkyl, alkoxy optionally substituted by fluorine, alkylthio, alkoxyalkoxy, dialkylamino, piperidino, morpholino, halogen, phenylalkyl, phenylalkoxy; R.sub.4 and R.sub.4, are the same or different and selected from hydrogen, alkyl, aralkyl; R.sub.5 is hydrogen, halogen, trifluoromethyl, alkyl, alkoxy; R.sub.6 -R.sub.9 are the same or different and selected from hydrogen, alkyl, alkoxy, halogen, haloalkoxy, alkylcarbonyl, alkoxycarbonyl, oxazolyl, trifluoroalkyl or adjacent groups R.sub.6 -R.sub.9 may complete together with the carbon atoms to which they are attached optionally substituted ring structures; R.sub.10 is hydrogen or alkcoxycarbonyloxymethyl; R.sub.11 is hydrogen or forms an alkylene chain together with R; R.sub.12 and R.sub.13 are the same or different and selected from hydrogen, halogen or alkyl; which method comprises stereoselective bioreduction of a compound of formula (II) in racemic form.

The compounds of formula (II) are active H.sup.+ K.sup.+ APTase inhibitors.

The compounds of formula (II) possess a stereogenic (asymmetric) centre which is the sulphur atom which forms the sulfoxide group between the Het.sub.1 -X-and Het.sub.2 moieties. The compounds of formula (II) generally are a racemic mixture initially.

In the method according to the present invention the starting compound of formula (II) in racemic form is stereoselectively bioreduced to the corresponding sulfide of the formula:

Het.sub.1 --X--S-Het.sub.2 (I) (wherein Het.sub.1, X and Het.sub.2 are as defined above). Thus there is obtained compound of formula (II) as a single enantiomer or in enantiomerically enriched form which may be separated from the sulfide produced.

In the above definitions allyl groups or moieties may be branched or straight chained or comprise cyclic alkyl groups, for example cycloalkylalkyl.

Preferably: ##STR7## and ##STR8## and ##STR9## (wherein R.sub.1, R.sub.2, R.sub.3, R.sub.6 -R.sub.9,R.sub.10 and R.sub.11 are as defined above).

Most preferably the compounds with which the method of the present invention is concerned are compounds of the formula (IIa) to (IIe): ##STR10##

An example of a compound of formula (II) wherein R.sub.10 is alkoxycarbonyloxymethyl is ##STR11##

The sulfides formed from the compounds (IIa)-(IIf) will be respectively ##STR12##

The stereoselective bioreduction according to the present invention may be carried out using a microorganism or an enzyme system derivable therefrom. The organisms used in the method according to the present invention are suitably organisms containing DMSO reductase, for example enterobacteriaceae such as E. Coli and Proteus sp., and purple non-sulfur bacteria of the genus Rhodobacter.

Also there may be used DMSO reductase to effect the bioreduction.

Using DMSO reductase, under anaerobic conditions a cofactor flavin adenine dinudeotide (FAD) or flavin mononudeotide (FMN) for example may be photoreduced in the presence of ethylene diamine tetraacetic acid (EDTA). The re-oxidation of the FAD or FMN is coupled to the reduction of the sulfoxide via the DMSO reductase. Hence an artificial biocatalytic cycle is generated in the absence of the cells natural anaerobic electron transport mechanism. ##STR13##

The compounds of formula (II) are generally add labile and thus the use of acid conditions is to be avoided. Generally the method according to the invention may be carried out at a pH of 7.6 to 8, suitably about 7.6, and at temperature of 25.degree. to 35.degree. C., suitably about 28.degree. C.

According to one embodiment of the invention the method comprises contacting the compound of formula (II) with a microorganism which is:

Proteus vulgaris Proteus mirabilis Escherichia coli Rhodobacter capsulatus

The microorganisms used are preferably:

Proteus vulgaris NCIMB 67 Proteus mirabilis NCIMB 8268 Escherichia coli ATCC 33694 Rhodobacter capsulatus DSM 938

These microorganisms are available from the following culture collections.

NCIMB National Collection Of Industrial And Marine Bacteria 23 Saint Machar Drive Aberdeen AB2 1RY United Kingdom ATCC American Type Culture Collection 12301 Parklawn Drive Rockville Maryland 20852 United States of America DSM Deutsche Sammlung von Mikroorganismen Mascheroder Weg 1b D-38124 Braunschweig Germany

The present invention will now be illustrated with reference to the Examples.

EXAMPLE 1

The reductive resolution of the compound of formula (IIa) was investigated using whole cells of E. Coli ATCC 33694, Proteus vulgaris NCIMB 67, Proteus mirabilis NCIMB 8268 and a preparation of DMSO reductase from Rhodobacter Capsulatus DSM 938. E. Coli and the two strains of Proteus were grown under essentially anaerobic conditions in 1 litre screw capped flasks containing 800 ml of medium at 35.degree. C. for 48 hours on a rotary shaker. The basal culture medium used had the following composition (g/l): NaH.sub.2 PO.sub.4, 1.56; K.sub.2 HPO.sub.4, 1.9; (NH.sub.4).sub.2 SO.sub.4, 1.8; MgSO.sub.4 .multidot.7H.sub.2 O, 0.2; FeCI.sub.3, 0.005; Na.sub.2 MoO.sub.4 .multidot.2H.sub.2 O, 0.001; casamino acids, 1.5. After autoclaving this was supplemented with the following components (g/l): glycerol (for Escherichia coli) or glucose (for Proteus mirabilis ), 5; thmine.multidot.HCl, 0.03; nicotinic acid, 0.007. Dimethylsulfoxide (70 mM) was added to the medium to serve as terminal electron acceptor for anaerobic respiration and as inducer for dimethylsulfoxide (DMSO) reductase. Trace elements solution (1 mi/litre) was also added to the medium. The stock trace elements solution contained (g/l): CuSO.sub.4 .multidot.5H.sub.2 O, 0.02; MnSO.sub.4 .multidot.4H.sub.2 O, 0.1; ZnSO.sub.4 .multidot.7H.sub.2 O, 0.1; CaCO.sub.3, 1.8. E. Coli was also grown anaerobically on the same medium under the same conditions but with 40 mM of fumarate as electron acceptor.

Cells were harvested by centrifuging and washed twice in 10 mM phosphate buffer, pH 7.6. The cells were then resuspended in 100 mM phosphate buffer, pH 7.6 to give a dry cell weight concentration of 3-6 g/l.

50 ml of each cell suspension was then placed in an autotitrator vessel and stirred without aeration in the presence of 0.1-0.3 g/l substrate and glucose (2.5%) at 35.degree. C. The pH was maintained at 7.6 by autotitration with 0.5 mM NaOH.

Cells of Rhodobacter capsulatus DSM 938 where grown and DMSO reductase enzyme prepared as describes in Example 2, below. The reductive resolution of the compound of formula (IIa) using said enzyme preparation was then carried out as describes in Example 2, except that the compound of formula (IIa) was present at a substrate concentration of 2.9 mM.

Detection of Products

The bioreduction of the compound of formula (IIa) was followed by reverse phase HPLC on a Spherisorb S5-ODS2 reverse phase column eluted with a 50:50 mixture of acetonitrile and 25 mM sodium phosphate buffer, pH 7.6 at a flow rate of 0.8 ml/mirL Under such conditions the compounds of formula (IIa) and (Ia) were well resolved with retention times of 5.2 and 9.8 minutes respectively. Both compounds were detected at a wavelength of 300 nm.

The enantiomeric composition of the compound of formula (IIa) remaining was investigated by the following method. After removal of biomass the aqueous media was extracted with two volumes of ammonia saturated dichloromethaie. The pooled organic extracts were dried over anhydrous sodium sulfate and the solvent was evaporated under reduced pressure to afford a pale brown solid. Then the enantiomeric composition of sulfoxide was determined by HPLC on a Chiralpak AD Column under the following conditions:

Column Chiralpack AD 250 mm.times.4.6 mm interior diameter with 50 mm guard column Eluent Hexane:Ethanol:Methanol (40:55:5% V/V) Flow 1.0 ml/min Injection Volume 20 .mu.l Wavelength 300 nm Retention times Compound of formula (Ia) 5.1 min Compound of formula (IIa) (+) Enantiomer 8.5 min (-) Enantiomer 13.4 min

The result in Table 1 were obtained for E. Coli ATCC 33694:

TABLE 1______________________________________ Dry Compound of Compound of Cell formula formula eeElectron Weight Time (IIa) (ppm (Ia) (ppm or %Acceptor g/l (min) or mg L.sup.-1) mg L.sup.-1) (+) E______________________________________Fumarate 6 0 76 0 -- -- 5 65 10 -- -- 15 42 33 -- -- 30 33 42 -- -- 40 30 45 -- -- 65 27 47 -- -- 90 25 50 -- -- 130 22 53 >99 11DMSO 6 0 74 0 0 -- 15 47 22 28 -- 35 40 34 74 -- 45 34 38 90 20______________________________________ In the above Table E is the enantiospecificity constant which may be determined from the extent of conversion and enantiomeric excess of the unreacted compound from the following equation: ##EQU1## where C=conversion ee.sub.s =enantiomeric excess of the unreacted compound.

Also in the above table ee is the enantiomeric excess value for the (+) enantiomer of the compound of formula (IIa). The enantiomeric excess value gives an indication of the relative amounts of each enantiomer obtained. The value is the difference between the relative percentages for the two enantiomers. Thus, for example, when the percentage of the (-) enantiomer of the remaining sulfoxide is 97.5% and the percentage for the (+) enantiomer is 2.5%, the enantiomeric excess for the (-) enantiomer is 95%.

At a starting concentration of 0.3 g/l both P. vulgaris NCIMB 67 and P. mirabilis NCIMB 8268 afforded the (+) enantiomer of the compound of formula (IIa) in >99% enantiomeric excess after 48 h (Table 2) in yields of 32% and 5% respectively.

TABLE 2______________________________________ P. vulgaris P. mirabilis NCIMB 67 NCIMB 8268 Ratio of Ratio of Enantiomers EnantiomersTime hours (+) (-) (+) (-)______________________________________0 50 50 50 5024 79 21 67 3348 >99 <1 >99 <1______________________________________

The final concentration of the compound of formula (IIa) after 48 hours was 48 mg/litre for P. vulgaris NCIMB 67 and 8 mg/litre for P. mirabilus NCIMB 8268.

At a lower starting concentration of 0.1 g/l both organisms again afforded the (+) enantiomer of the compound of formula (IIa) in >99% enantiomeric excess, but after 24 hr (Table 3A) in 44% and 40% yields for P. vulgaris NCIMB 67 and P. mirabilis NCIMB 8268 respectively.

TABLE 3A______________________________________ P. vulgaris P. mirabilis NCIMB 67 NCIMB 8268 Ratio of Ratio Enantiomers of EnantiomersTime hours (+) (-) (+) (-)______________________________________0 50 50 50 505 78 22 79 2124 >99 <1 >99 <1______________________________________

The final concentration of the compound for formula (IIa) after 24 hours was 22 mg/litre of P. vulgaris NCIMB 67 and 20 mg/litre for P. mirabilis NCIMB 8268.

The compound of formula (IIa) acts as a substrate for the isolated DMSO reductase from Rhodobacter capsulatus as shown in Table 3B. The conversion of total suloxide after 15 minutes and after 1 hour was 80% and >90% respectively.

TABLE 3B______________________________________ R. capsulatus DSM 938 Ratio of EnantiomersTime (+) (-)______________________________________0 50 5015 minutes 50 501 h 15 85______________________________________

EXAMPLE 2

The reductive resolution of compounds of formula (IIb) and (IIc) was investigated with E. coli ATCC 33694, P. vulgaris NCIMB 67, and a preparation of DMSO reductase from R. capsulatus DSM 938. The reaction conditions were as described in Example 1 for E. coli and P. vulgaris except the compounds of formula (IIb) and (IIc) were present at a substrate concentration of 0.1 g/l. Where a preparation of DMSO reductase was used, the reaction conditions were as follows:

Cells of R. capsulatus DSM 938 were grown phototrophically in 25l carboys containing RCV medium supplemented with 50 mM DMSO between two banks of 100 W tungsten bulbs. Cells were harvested by cross-flow filtration followed by centrifugation at 10,000 rpm in a Beckman GSA rotor for 30 min. The pellets were washed once with 50 mM Tris/HCl pH 8.0 and resuspended to approximately 2 g/ml in 50 mM Tris/HCl pH 8.0+0.5 M sucrose and 1.5 mM EDTA (STE buffer). Lysozyme was added at 0.6 mg/ml and the suspension (approx 1l) was stirred at 30.degree. C. for 15 min. After centrifugation as described above the supernatant (periplasmic fraction) was decanted off and brought to 50% saturation with ammonium sulphate. Following centrifugation the supernatant was brought to 70% saturation and recentifuged. The 50-70% pellet was resuspended in a minimum volume of 50 mM Tris/HCl pH 8.0 and dialysed against 3.times.100 volumes of the same buffer being concentrated to approx 5 ml by ultra-filtration through an Amicon PM 10 membrane. The concentrated sample was brought to 150 mM NaCl, charged onto a Sephacryl S200 gel filtration column (column vol=510 ml) and eluted with 700 ml of 50 mM Tris/HCl pH 8.0+150 mM NaCl. Peak enzyme activity eluted at approx 320 ml (data not shown) and peak fractions were pooled and concentrated as above.

The "RCV" medium used in this Example had the following composition (g per litre of deionised water or as mM):

Propionate 30 mM (NH.sub.4).sub.2 SO.sub.4 1 g KPO.sub.4 buffer 10 mM MgSO.sub.4 *7H.sub.2 O 120 mg CaCl.sub.2 *2H.sub.2 O 75 mg Sodium EDTA 20 mg FeSO.sub.4 *7H.sub.2 O 24 mg Thiamine hydrochloride 1 mg Trace element soln. 1 ml Trace element solution (per 250 ml) H.sub.3 BO.sub.3 0.7 g MnSO.sub.4 *H.sub.2 O 398 mg Na.sub.2 MoO.sub.4 *2H.sub.2 O 188 mg ZnSO.sub.4 *7H.sub.2 O 60 mg Cu(NO.sub.3).sub.2 *3H.sub.2 O 10 mg

The puzified DMSO reductase from Rhodobacter capsulatus DSM 938 was used in the photo-catalysed assay for the reduction of sulfoxide. A 5 ml gas-tight syringe was filled with approx 3 ml of degassed assay buffer, 50 mM Tris/HCl, 10 mM EDTA, pH 8.0. Flavin mononudeotide (FMN) and sulfoxide were added to the syringe to give final concentrations of 250 .mu.M and 0.3 mM respectively (based on a final volume of 5 ml). The FMN was then reduced via illumination with a tungsten lamp. The DMSO reductase sample (2-10 mg: 2-10 .mu.M) was then added to the solution and the volume made up to 5 ml with degassed buffer. The syringe was then illuminated as before. A 1 ml sample was removed immediately (t=o) and subsequent sampling repeated over the desired timecourse.

Detection of Products

The bioreduction of the compounds of formula (IIb) and (IIc) was followed by reverse phase HPLC as described in Example 1 except that the retention times were as follows:

TABLE 4______________________________________Compound of Formula Retention Time (min)______________________________________Ib 8.1IIb 4.2Ic 10.5IIc 5.7______________________________________

The enantiomeric composition of the compounds of formula (IIb) and (IIc) remaining was investigated by the method of Example 1 except that in he chiral HPLC step, the solvent composition, flow rate and retention time were as follows:

TABLE 5______________________________________Compound of Solvent Flow Rate Retention timeFormula Composition (ml/min) (min)______________________________________IIb Hexane/Ethanol 1.0 32.3 (Enantiomer A) (70:30% v/v) 36.6 (Enantiomer B)IIc Hexane/Ethanol 0.5 34.3 (Enantiomer A) (70:30% v/v) 36.3 (Enantiomer B)______________________________________ The enantiomer which eluted first is referred to as enantiomer A and the second as enantiomer B.

The following results were obtained:

TABLE 6______________________________________ Ratio of enantiomers of formula (IIb)Biocatalyst Time (hr) A B Conversion (%)______________________________________E coli ATCC 33694 0 50 50 -- 2 45 55 68 20 40 60 -- 44 <1 >99 89P vulgaris NCIMB 67 0 50 50 -- 2 53 47 75 6 53 47 75 48 53 47 75______________________________________

Conversion values were for the percentage conversion of the compound of formula (IIb) to the compound of formula (Ib).

TABLE 7______________________________________ Ratio of enantiomers of formula (IIc)Biocatalyst Time (hr) A B Conversion (%)______________________________________E. Coli ATCC 33694 0 50 50 -- 2 39 61 83 20 21 79 96P. Vulgaris NCIMB 67 0 50 50 -- 2 40 60 45 6 40 60 54 48 38 62 85DMSO reductase (R. 0 50 50 --capsulatus DSM 938) 0.25 41 59 31 1.5 41 59 54______________________________________

Conversion values were the percentage conversion of the compound of formula (IIc) to compound of formula (Ic).

EXAMPLE 3

The reductive resolution of compounds of formula (IId) and (IIe) was investigated.

E. coli ATCC 33694 and Proteus mirabilis NCIMB 8268 were screened for the reductive resolution of compounds of formula (IId) and (IIe). Both organisms were grown anaerobically with fumarate as terminal electron acceptor and glycerol (E. coli ) or glucose (Proteus mirabilis ) as carbon source according to the method of Example 1. After 48 hrs growth at 35.degree. C. the cells were harvested by centrifuging at 8k rpm and 4.degree. C. and washed by resuspending in 10 mM sodium phosphate buffer, pH 7.6 and centrifuging as above. The cells were finally resuspended in 100 mM sodium phosphate buffer, pH 7.6. The dry cell weights were 8 g/l (E. coli ) and 4.5 g/l (Proteus mirabilis ) for the compound of formula (IId) and 4.3 g/l (E. coli ) and 3.9 g/l (Proteus mirabilis ) for the compound of formula (IIe). The reductive resolution of the compounds of formula (IId) and (IIe) was investigated in an autotitrator containing 40 ml cell suspension, 100 ppm of either substrate and 1% w/v glucose as energy source.

Detection of Products

The bioreduction of the compounds of formula (IId) and (IIe) was followed by reverse phase HPLC as described in Example 1 except that the retention times were as follows:

TABLE 8______________________________________Compound of formula Retention Time (min)______________________________________Id 13.7IId 5.0Ie 9.4IIe 4.3______________________________________ The enantiomeric composition of the compounds of formula (IId) and (IIe) remaining was investigated by the method of Example 1 except that in the chiral HPLC step, the solvent composition, flow rate and retention time were as follows: TABLE 9______________________________________Compound Solvent Flow Rate Retention Timeof Formula Composition (ml/min) (min)______________________________________IId Hexane/Ethanol 1.0 12.9 (Enantiomer A) (70:30% v/v) 21.7 (Enantiomer B) Hexane/Ethanol/ 1.0 7.4 (Enantiomer A) Methanol (40:55:5% v/v) 10.6 (Enantiomer B)IIe Hexane/Ethanol 1.0 26.0 (Enantiomer A) (70:30% v/v) 30.5 (Enantiomer B)______________________________________ The enantiomer which eluted first is referred to as enantiomer A and the second as enantiomer B.

The results for the reduction of the compound of formula (IId) by both E. coli ATCC 33694 and P. mirabilis NCIMB 8268 were as follows:

TABLE 10______________________________________ EnantiomericMicroorganism Conversion (%) excess (%) Enantiomer E______________________________________E. Coli ATCC 33694 79 88 B 4P. mirabilis 66 90 B 8NCIMB 8268______________________________________ The conversion values were for the percentage conversion of compound of formula (IId) to compound of formula (Id).

The reduction of the compound of formula (IIe) by E. coli and P. mirabilis afforded `B` enantiomer in high enantiomeric excess.

TABLE 11______________________________________ EnantiomericMicroorganism Conversion (%) excess (%) Enantiomer E______________________________________E. Coli ATCC 33694 78 98.2 B 7P. mirabilis 70 >99 B 11NCIMB 8268______________________________________

The conversion values were for the percentage conversion of the compound of formula (IIe) to the compound of formula (Ie).

These conversion figures were determined from the aqueous dissolved concentration of sulfoxide at the end of the reaction. The magnitude of the enantiospecificity constant is similar to that obtained for the reductive resolution of the compound of formula (IIa) by E. coli ATCC 33694 and Proteus mirabilis NCIMB 8268.

Claims

1. A method of obtaining a pharmaceutically active compound as a single sulfoxide enantiomer or an enantiomerically enriched sulfoxide form having the formula (II): ##STR14## wherein: ##STR15## and ##STR16## and ##STR17## wherein: N in the benzimidazole moiety of Het.sub.2 means that one of the carbon atoms substituted by any one of R.sub.6 to R.sub.9 is exchanged for an unsubstituted nitrogen atom;

R.sub.1, R.sub.2 and R.sub.3 are the same or different and selected from the group consisting of hydrogen, alkyl, alkoxy which is unsubstituted or substituted by fluorine, alkylthio, alkoxyalkoxy, dialkylamino, piperidino, morpholino, halogen, phenylalkyl and phenylalkoxy;

R.sub.4 and R.sub.4 are the same or different and selected from the group consisting of hydrogen, alkyl and aralkyl;

R.sub.5 is hydrogen, halogen, trifluoromethyl, alkyl or alkoxy;

R.sub.6 -R.sub.9 are the same or different and selected from the group consisting of hydrogen, alkyl, alkoxy, halogen, haloalkoxy, alkylcarbonyl, alkoxycarbonyl, oxazolyl and trifluoroalkyl;

or adjacent groups among substituents R.sub.6 -R.sub.9 together with the carbon atoms to which they are attached form an unsubstituted or a substituted ring;

R.sub.10 is hydrogen or alkoxycarbonyloxymethyl;

R.sub.11 is hydrogen or forms an alkylene chain together with R.sub.3 ;

R.sub.12 and R.sub.13 are the same or different and selected from the group consisting of hydrogen, halogen and alkyl;

which method comprises the steps of:

enantioselectively bioreducing a racemic sulfoxide compound of the formula (II) to the corresponding sulfide compound of formula (I):

Het.sub.1 -X-S-Het.sub.2 (I)

by means of a microbial organism or a microbial enzyme system; and

isolating the pharmaceutically active single enantiomeric or enantiomerically enriched sulfoxide compound.

2. A method according to claim 1 wherein: ##STR18## and ##STR19## and ##STR20##

3. A method according to claim 1 or 2 wherein the compound of formula (II) is a H.sup.+ K.sup.+ -ATPase enzyme inhibitory compound of formula: ##STR21##

4. A method according to any one of the previous claims wherein a single enantiomer of the compound of formula (II) is obtained.

5. The method according to claim 3, wherein the racemic sulfoxide compound of formula (IIa) is enantioselectively bioreduced to an enantiomeric or enantiomerically enriched sulfide form of formula (Ia) which is effected by the action of Proteus vulgaris, Proteus mirabilis, Escherichia coli, Rhodobacter capsulatus or a DMSO reductase isolated from R. capsulatus ; and

separated from the sulfoxide racemate.

6. The method according to claim 3, wherein the racemic sulfoxide compound of formula (IIb) is enantioselectively bioreduced to an enantiomeric or enantiomerically enriched sulfide form of formula (Ib) which is effected by the action of Escherichia coli; and

separated from the sulfoxide racemate.

7. The method according to claim 3, wherein the racemic sulfoxide compound of formula (IIc) is carried out enantioselectively bioreduced to an enantiomeric or enantiomerically enriched sulfide which is effected by the action of Escherichia coli; and

separated from the sulfoxide racemate.

8. The method according to claim 3, wherein the racemic sulfoxide compound of formula (IId) or (IIe) is enantioselectively bioreduced to an enantiomeric or enantiomerically enriched corresponding sulfide compound of formula (Id) or (Ie) respectively by the action of of Proteus mirabilis or Escherichia coli; and

separated from the sulfoxide racemate.

9. A method for the preparation of a pharmaceutically active enantiomeric or enantiomerically enriched sulfoxide compound of formula II as defined in any one of the claims 1-3, comprising the steps of:

(a) enantioselectively bioreducing a racemate sulfoxide compound to its sulfide form of formula I as defined using whole cells of A. coli ATCC 33694, P. vulgaris NCI MB67, P. mirabilis NCI MB 8268, or using a preparation of DMSO reductase enzyme from Rhodobacter capsulatus DSM938; and

(b) isolating the pharmaceutically active enantiomeric or enantiomerically enriched sulfoxide compound.