Patent Application
20080081932
Weigh 30 g xanthophyll crystal from saponification of marigold oleoresin (wherein the total carotenoid is 88.5% and the proportions of lutein and zeaxanthin account for 92% and 7% of total carotenoid, respectively, the rest being a few other carotenoids). Mix with 540 ml glycol and stir and dissolve for 1.0 hr under 80° C. Heat up the mixture to 90° C. after dissolution is completed, and instill 40 g 6.85 mol/L sodium methoxide solution, and addition of alkali catalyst is to be completed within 45 min. Reaction is to take place at this temperature, and the lutein and zeaxanthin in the reaction solution are to be sampled at an interval of 0.5 hr after reaction for 4.0 hr and analyzed with high performance liquid chromatographic method for their proportions, to get a measurement of 89.7% proportion of zeaxanthin of total carotenoid in the reaction liquid after 8.5 hr. Drop the temperature of reaction mixture to 70° C., and add a mixture composed of 100 ml deionized water and 600 ml food-grade ethanol under the stirring condition to dilute the reaction substance. While separating crystals from the diluent mixed solution with centrifugal method, a mixture of water and ethanol is used for spraying rinse, until the washed out liquid is nearly colorless. 21.3 g tangerine colored crystal can be obtained for the final filtrated matter through vacuum drying. Analyzed by ultraviolet visible light spectrophotometric method, the total carotenoid content in this crystal is 85.7%. The yield of total carotenoid is 68.6%. Analyzed with high performance liquid chromatographic method, the zeaxanthin wherein account for 91.2% of total carotenoid and the lutein occupies 6.1% of total carotenoid.
No toxic organic solvent is contained in the products, suitable to be used in the forms of nutrition extender and food additive. The application form of this crystal can be oil suspension (mixed and emulsified with plant oil), granule beads (microcapsule condensed through spray condensation granule beads technology), and dry powder (microcapsule through spray-drying), etc.
Operating conditions and product characters in this example are listed in Table 1.
Weigh 40 g xanthophyll fatty acid ester refined from marigold oleoresin (wherein the carotenoid fatty acid ester content of total carotenoid is 72.5%, and the proportions of lutein and zeaxanthin in total carotenoid are 91.6% and 6.8%, respectively, the rest being a few other carotenoids). Fully mix xanthophyll fatty acid ester with 470 ml propylene glycol and dissolve the mixture under 70° C. Heat up to 85° C. after dissolution is completed. Add 110 g 2.92 mol/L sodium tertiary butyl alcohol solution drop by drop, and it is determined after reaction standing for 14.5 hr with high performance liquid chromatographic method that zeaxanthin wherein accounts for an 84.6% proportion of the total carotenoid. Cool down to 70° C. and add a mixed liquid composed of 1000 ml deionized water and 600 ml food-grade ethanol to dilute the reaction liquor. Extract and filter out the crystals from separation, with a mixture of water and ethanol for washing during the extracting and filtering process, until the washed out liquid is nearly colorless. 10.3 g saffron colored crystal is gained from the filtrated insoluble substance after vacuum drying. Analyzed with ultraviolet visible light spectrophotometric method, the total carotenoid content in the crystal is 81.3%, while the yield of total carotenoid is 54.0%. Through HPLC analysis, zeaxanthin in the product accounts for 85.2% of the total carotenoid, and the lutein occupies 11.3% of the total carotenoid, both in dissociative forms.
Operating conditions and product characters in this Example are listed in Table 1.
Implementation processes for Examples 3-4 are similar to those of Examples 1-2, and the analytical results for their main technical parameters and some product characters are listed in Table 1.
| Number | Date | Country | Kind |
|---|---|---|---|
| 200610053642.3 | Sep 2006 | CN | national |
