Patent Application
20240009644
The present invention relates to a method for preparing multilayered spherical particles. More specifically, the present invention relates to a method for preparing multilayered spherical particles comprising: i) preparing a core component comprising an active ingredient and a shell solution comprising a first polymer; ii) forming core-shell particles by electro-coextrusion of the core component and the shell solution of step (i); iii) drying the core-shell particles obtained in step (ii); and iv) encapsulating the dried core-shell particles of step (iii) with a second polymer.
In addition, the present invention relates to a cosmetic composition comprising multilayered spherical particles prepared by the above method.
Recently, as cosmetics have become diversified, consumers have begun to demand aesthetic functions as well as efficacy in cosmetics. As such, cosmetic developers are making efforts to create aesthetically superior products as well as multifunctional, easy-to-use and effective products.
Meanwhile, as one method for delivering active ingredients more stably, there is an encapsulation technology. Encapsulation is a technique in which functional materials are entrapped in a small structure made of polymer to achieve physicochemical stability, volatility and odor suppression, dispersibility improvement, application range expansion through solidification, and toxicity reduction and functional effect increase due to release control. Such an encapsulation structure usually has a capsule form composed of a core-shell or a particle form composed of a core-matrix. Such encapsulation technology has not yet been actively applied in the field of cosmetics.
Accordingly, the technical problem of the present invention is the provision of a method for preparing multilayered spherical particles that can efficiently deliver cosmetic ingredients into the skin in a stable form and give excellent aesthetics.
In addition, another technical problem of the present invention is the provision of a cosmetic composition comprising multilayered spherical particles prepared by the above method.
To solve the above technical problem, the present invention provides a method for preparing multilayered spherical particles comprising:
In addition, to solve another technical problem, the present invention provides a cosmetic composition comprising multilayered spherical particles prepared by the above method.
The present invention is described in detail hereinafter.
According to one aspect to the present invention, there is provided a method for preparing multilayered spherical particles comprising: i) preparing a core component comprising an active ingredient and a shell solution comprising a first polymer; ii) forming core-shell particles by electro-coextrusion of the core component and the shell solution of step (i); iii) drying the core-shell particles obtained in step (ii); and iv) encapsulating the dried core-shell particles of step (iii) with a second polymer.
In step (i) of the method for preparing multilayered spherical particles according to the present invention, a core component comprising an active ingredient and a shell solution comprising a first polymer component are prepared.
In one embodiment according to the present invention, the active ingredient, for example, may be selected from the group consisting of oil, wax, hydrocarbon, higher fatty acid, higher alcohol, silicone, ester and a mixture thereof, but is not limited thereto.
In another embodiment according to the present invention, examples of the oil may include macadamia nut oil, olive oil, jojoba oil, sunflower seed oil, argan oil, camellia oil, avocado oil, soybean oil, grape seed oil, castor oil, rice bran oil and the like, but are not limited thereto.
In another embodiment according to the present invention, examples of the wax may include carnauba wax, candelilla wax, jojoba oil, beeswax, lanolin and the like, but are not limited thereto.
In another embodiment according to the present invention, examples of the hydrocarbon may include liquid paraffin, paraffin, Vaseline™ (petroleum jelly), ceresin, microcrystalline wax, squalane and the like, but are not limited thereto.
In another embodiment according to the present invention, examples of the higher fatty acid may include lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid and the like, but are not limited thereto.
In another embodiment according to the present invention, examples of the higher alcohol may include cetyl alcohol, stearyl alcohol, isostearyl alcohol, 2-octyldodecyl alcohol, diisostearyl malate and the like, but are not limited thereto.
In another embodiment according to the present invention, examples of the silicone may include dimethicone (polydimethylsiloxane), cyclomethicones, silicone polymers, silicone oil and the like, but are not limited thereto.
In another embodiment according to the invention, examples of the ester may include cetyl ethylhexanoate, PEG-20 glyceryl triisostearate, capric/caprylic triglyceride, glyceryl tri(2-ethylhexanoate), isononyl isononanoate, ethylhexyl isononanoate, ethylhexyl palmitate, isostearyl isostearate, neopentyl glycol dicaprate, neopentyl glycol diethylhexanoate, octyldodecyl myristate, pentaerythrityl tetraethylhexanoate, pentaerythrityl tetraisostearate, isotridecyl isononanoate, trimethylopropane triisostearate, squalene and the like, but are not limited thereto.
In another embodiment according to the present invention, as the active ingredient, an oil-soluble cosmetic ingredient—for example, retinol, retinal, glycyrrhizic acid, cica powder, coenzyme 010, astaxanthin, idebenone, conjugated linoleic acid (CLA), vitamin E (tocopherol), vitamin D, linolenic acid, biotin and the like may be used.
In another embodiment according to the present invention, the active ingredient may form a complex with a covalent organic framework (COF). A covalent organic framework is a two-dimensional or three-dimensional organic solid having an extended structure, in which building blocks are linked by strong covalent bonds. In another embodiment according to the present invention, the active ingredient may form a complex with a covalent organic framework based on, for example, cyclodextrin.
In another embodiment according to the present invention, the first polymer may be selected from the group consisting of nanocellulose, cellulose, hydroxyethyl cellulose, microcrystalline cellulose, starch, polymethyl methacrylate (PMMA), xanthan gum, carbomer, acrylates/C10-30 alkyl acrylate crosspolymer, acacia gum, guar gum, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), gelatin, hyaluronic acid, collagen, pullulan, polyglycolide (PGA), agar, gum arabic, carrageenan gum, gellan gum, karaya gum, methylcellulose, locust bean gum, alginate, sodium alginate, glucomannan, succinyl chitosan, pullulan lactide, chitosan, polyethylene glycol (PEG)-polycaprolactone (PCL)-polyethylene glycol (PEG) triblock copolymer, poloxamer 407 (Pluronic™ F-127), acetyl hydroxypropyl cellulose and a mixture thereof, but is not limited thereto.
In another embodiment according to the present invention, as the first polymer a mixture of two or more types of polymers may be used.
In another embodiment according to the present invention, as the first polymer a mixture of three or more types of polymers may be used.
In another embodiment according to the present invention, the concentration of the first polymer in the shell solution may be 1 to 50% by weight, 2 to 45% by weight or 5 to 40% by weight.
In step (ii) of the method for preparing multilayered spherical particles according to the present invention, core-shell particles are formed by electro-coextrusion of the core component comprising an active ingredient and the shell solution comprising a first polymer.
In electro-coextrusion, different materials are expelled from two coaxial capillaries under an electric field to form a core-shell capsule (
In another embodiment according to the present invention, the electro-coextrusion of the core component may be carried out at a flow rate of 0.1 to 10 mL/min, 0.2 to 8 mL/min or 0.5 to 5 mL/min.
In another embodiment according to the present invention, the electro-coextrusion of the shell solution may be carried out at a flow rate of 0.5 to 20 mL/min, 1 to 15 mL/min or 3 to 12 mL/min.
In another embodiment according to the present invention, the electro-coextrusion may be carried out at 1,000 to 5,000 volts (V), 1,500 to 4,000 volts (V) or 2,000 to 3,000 volts (V).
In another embodiment according to the present invention, a calcium chloride solution may be used as a curing agent in the electro-coextrusion.
In step (iii) of the method for preparing multilayered spherical particles according to the present invention, the core-shell particles obtained in step (ii) are dried.
In another embodiment according to the present invention, drying of the core-shell particles may be carried out at 60 to 90° C.
In step (iv) of the method for preparing multilayered spherical particles according to the present invention, the dried core-shell particles obtained in step (iii) are encapsulated with a second polymer to obtain multilayered spherical particles.
The encapsulation with the second polymer may be carried out by a method known in the art and there is no particular limitation thereto.
In another embodiment according to the present invention, the second polymer may be polymers that can act as cosmetic ingredients—for example, may be selected from the group consisting of collagen, hyaluronic acid, pullulan, proteoglycan, beta-glucan, glucan, polyglutamic acid, chitosan and a mixture thereof, but is not limited thereto.
In another embodiment according to the present invention, the multilayered spherical particles prepared by the above method may have a diameter of 200 to 1,500 μm.
According to another aspect of the present invention, there is provided a cosmetic composition comprising multilayered spherical particles prepared by the above method.
In the present invention, the cosmetic composition may be formulated into various products such as face lotion, emulsion, body lotion, cream, essence, ampoule and BB (blemish balm) cream, but is not limited thereto. In the present invention, the cosmetic composition may include various amounts of multilayered spherical particles according to the needs of the formulation—for example, the multilayered spherical particles may be comprised in an amount of 0.1 to 50% by weight.
According to the present invention, multilayered spherical particles can be prepared with excellent encapsulation efficiency and high production efficiency of various active ingredients. The multilayered spherical particles prepared according to the present invention can not only efficiently deliver active ingredients into the skin in a stable form, but also have good appearance to give excellent aesthetic sensibility.
Hereinafter, the present invention is explained in more detail with the following examples. However, it must be understood that the protection scope of the present invention is not limited to the examples.
A shell solution was prepared from Ingredient A according to the composition recited in Table 1, and a solution was prepared by dissolving a core component of Ingredient B and 1% calcium chloride as a curing agent in purified water. Then, electro-coextrusion was carried out with the above solutions by the use of Encapsulator B-390 (Buchi, Switzerland) under the following conditions to obtain core-shell particles.
After drying the obtained core-shell particles at 80° C. for 12 hours, the dried core-shell particles were put in a container, and Ingredient C was slowly added thereto. Then, encapsulation was carried out by mixing them with a winged disperser for 10 minutes to prepare multilayered spherical particles.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 2.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 3.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 4.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 5.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 6.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 7.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 8.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 9.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 10.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 11.
Multilayered spherical particles were prepared by the same method as described in Example 1 with the constitutional composition of Table 12.
With the constitutional composition of Table 13, each ingredient was introduced into a container and dissolved at a temperature of 80° C., then mixed for 5 minutes using a homo-mixer, cooled and deaerated to obtain a lotion.
With the constitutional composition of Table 14, each ingredient was introduced into a container and dissolved at a temperature of 80° C., then mixed for 5 minutes using a homo-mixer, cooled and deaerated to obtain a body lotion.
With the constitutional composition of Table 15, each ingredient was introduced into a container and dissolved at a temperature of 80° C., then mixed for 5 minutes using a homo-mixer, cooled and deaerated to obtain a cream.
With the constitutional composition of Table 16, each ingredient was introduced into a container and dissolved at a temperature of 80° C., then mixed for 5 minutes using a homo-mixer, cooled and deaerated to obtain an essence.
With the constitutional composition of Table 17, each ingredient was introduced into a container and dissolved at a temperature of 80° C., then mixed for 5 minutes using a homo-mixer, cooled and deaerated to obtain a transparent essence.
Ingredient A was prepared by three (3)-time treatment with a triple roller mill device. Ingredient B was put into the manufacturing section and heated to 75 to and then Ingredient A treated with a triple roller mill device was added thereto and dispersed. Ingredient C was dissolved in a separate container, heated to 80 to 85° C. while stirring with a homo-mixer. Ingredient C was added to the above prepared ingredients, and the mixture was emulsified for 10 minutes. After emulsification was completed, the obtained product was cooled to 45° C. while stirring using a stirrer, then cooled to 25° C. again, and then put in a container and matured.
The particle size of the multilayered spherical particles prepared in Example 1 was measured by the use of a particle size analyzer (Mastersizer 2000, MLAVERN, UK), and the result is represented in
The change in retinol content in the lotions of Example 13 and Comparative Example was quantitatively analyzed at 7-day intervals for one month while maintaining light-shielding conditions at 25° C. and light-exposure conditions at 40° C. As an equipment HPLC (Waters, USA) was used, and the analysis conditions were as follows. The analyzed results are represented in Table 19, and light-exposure conditions at 40° C. are described in parentheses.
The artificial skin, Neoderm (Tego Science, Korea) was mounted to a Franz-type diffusion cell (Lab Fine Instruments, Korea). 50 mM phosphate buffer (pH 7.4, 0.1 M NaCl) was added to a receptor cell (5 ml) of the Franz-type diffusion cell. A diffusion cell was then mixed and diffused at 600 rpm, 32° C., and 50 μl of the lotions of Example 13 and Comparative Example, respectively, were added to donor cells. Absorption and diffusion were carried out according to the predetermined time, and the area of the skin where the absorption and diffusion were carried out was 0.64 cm2. After finishing the absorption and diffusion of the active ingredient, the residues—which were not absorbed and remained on the skin—were cleaned with dried Kimwipes™ or 10 ml of ethanol. The skin in which the active ingredient was absorbed and diffused was homogenized by the use of a tip-type homogenizer, and retinol absorbed into the skin was then extracted with 4 ml of dichloromethane. The extract was then filtrated with a 0.45 μm nylon membrane filter. The content of retinol was measured by high-performance liquid chromatography (HPLC) with the following conditions, and the results are represented in Table 20.
As can be seen from Table 20, it was confirmed that the lotion according to the present invention has superior transdermal absorption compared to the general lotion.
After applying the lotions of Example 13 and Comparative Example, the moisturizing ability was measured by Corneometer CM850 (Courage+Khazaka electronic GmbH, Germany), and the results are represented in Table 21. As can be seen from Table 21, it can be known that the lotion containing the multilayered spherical particles has superior moisturizing ability than the lotion without them.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2020-0161247 | Nov 2020 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2021/017466 | 11/25/2021 | WO |
