METHOD FOR PREPARING POLYION THIN FILM, AND POLYION THIN FILM AND DETECTION KIT

Abstract

The present invention provides a method for preparing a polyion thin film (in particular a polyion thin film with different surface morphologies) and the polyion thin film prepared by the method. The method comprises: providing a substrate, and coating a first lubricant on a surface of the substrate or on a surface of a tinfoil covered on the surface of the substrate; covering a film-forming liquid of the polyion thin film on the surface of the substrate with the first lubricant, and after coating uniformly, covering a cover plate coated with a second lubricant on the film-forming liquid; performing a polymerization reaction of ionic liquid monomers in the film-forming liquid to form a polyion thin film; and separating the polyion thin film and cleaning it to obtain the polyion thin film. The polyion thin films with different surface morphology can be prepared. The present invention further provides a detection kit.

Description

Claims

1. A method for preparing a polyion thin film, comprising: providing a substrate, and coating a first lubricant on a surface of the substrate or on a surface of a tinfoil covered on the surface of the substrate;covering a film-forming liquid of the polyion thin film on the surface of the substrate or on the surface of the tinfoil, and after coating uniformly, covering a cover plate coated with a second lubricant on the film-forming liquid;performing a polymerization reaction of ionic liquid monomers in the film-forming liquid to form the polyion thin film;separating the polyion thin film and cleaning it to obtain the polyion thin film.

2. The method of claim 1, wherein the substrate comprises a silicon wafer, which is the silicon wafer with a patterned surface; or, the surface of the silicon wafer comprises convex ridges, or the surface of the silicon wafer comprises concave grooves, or the surface of the silicon wafer is provided with protruding cylinders, or the surface of the silicon wafer is provided with circular holes penetrating through the silicone sheet.

3. The method of claim 1, wherein the first lubricant and the second lubricant are inert to ultraviolet light and do not interfere with ultraviolet light; or, the first lubricant is selected from one or more of white vaseline, silicone oil, paraffin, mineral oil and grease.

4. The method of claim 1, wherein the film-forming liquid comprises one or more of an imidazole ionic liquid, a pyridine ionic liquid, a quaternary ammonium salt ionic liquid, a quaternary phosphine ionic liquid and a pyrrolidine ionic liquid; or, the film-forming liquid comprises one or more of 1-ethyl-3-methylimidazolium tetrafluoroborate, 1-ethyl-3-methylimidazolium chloride, N-ethyl pyridine bromide, tributylmethylammonium chloride, tributylethylphosphonium bromide and N-butyl-N-methyl pyrrolidine bromide.

5. The method of claim 4, wherein the cover plate is a glass plate or a hard plastic plate that is resistant to ultraviolet light; or, the second lubricant is selected from white vaseline, silicone oil, paraffin, mineral oil, and grease.

6. The method of claim 1, wherein the polymerization reaction is carried out under the irradiation of ultraviolet light; the wavelength of the ultraviolet light is 250 nm-400 nm; andthe irradiation time of the ultraviolet light is 15 min-30 min.

7. The method of claim 1, wherein the polymerization reaction is carried out at a temperature of 20° C. -60° C.; or, the polymerization reaction is initiated by an initiator selected from the group consisting of photoinitiator 907, photoinitiator 184, azobisisobutyronitrile, 2,4,6-(trimethylbenzoyl) diphenylphosphine oxide or benzoin and derivatives thereof.

8. The method of claim 1, wherein separating the polyion thin film comprises: placing the cover plate attached with the polyion thin film into water for standing; or, the cleaning comprises ultrasonic cleaning in clear water, absolute ethyl alcohol and clear water in turn.

9. The method of claim 1, further comprising: wetting the substrate with a solvent before laying the tinfoil on the substrate; the solvent is water, ethyl alcohol or a mixed solvent thereof.

10. The method of claim 1, wherein the film-forming liquid is prepared by the following steps: step a, fully mixing the ionic liquid monomers; andstep b, adding a cross-linking agent and an initiator, and then carrying out an ultrasonic treatment to obtain the film-forming liquid.

11. The method of claim 10, wherein the step a further comprises: performing ultrasonic treatment on obtained mixed solution after the ionic liquid monomers are fully mixed; and the ultrasonic treatment is performed for 10 min-30 min; and wherein the step b, the ultrasonic treatment is performed for 10 min-30 min.

12. The method of claim 10, wherein the ionic liquid monomers comprise bromobutane, vinylimidazole and acrylonitrile; or, a molar ratio of the bromobutane to the vinylimidazole is 2:1 to 1:1;or, the mass of the acrylonitrile is greater than or equal to the sum of the mass of the bromobutane and the vinylimidazol.

13. The method of claim 12, wherein the cross-linking agent is N, N-Methylenebisacrylamide, and the initiator is 2,4,6-(trimethylbenzoyl) diphenylphosphine oxide; or, the mass of the cross-linking agent is 8wt%-12wt% of the total mass of the bromobutane, the vinylimidazole and the acrylonitrile, and the mass of the initiator is 0.5 wt%-2 wt% of the total mass of the bromobutane, the vinylimidazole and the acrylonitrile.

14. The method of claim 10, wherein the film-forming liquid is prepared by the following steps: mixing the bromobutane and the vinylimidazole in equal molar ratio, and carrying out an ultrasonic treatment on obtained mixed solution for 15 min until the two are fully mixed; adding an amount of the acrylonitrile equal to the total mass of the bromobutane and the vinylimidazole; adding an amount of N, N-Methylenebisacrylamide that is 8 wt% of the total mass of thebromobutane, the vinylimidazole and the acrylonitrile and an amount of 2,4,6-(trimethylbenzoyl) diphenylphosphine oxide that is 1.0 wt% of the total mass of the bromobutane, the vinylimidazole and the acrylonitrile, and then carrying out the ultrasonic treatment for 15 min to obtain the film-forming liquid.

15. The method of claim 1, further comprising: immersing the polyion thin film in a dye solution with a dye;the dye is selected from one of bromocresol green, cresol red and methyl orange;or, the dye solution is a solution obtained by dissolving the dye in a mixed solvent of water and ethyl alcohol, wherein in the mixed solvent of water and ethyl alcohol, the volume ratio of water to ethyl alcohol is 4:1-1:8, or 1:4;or, in the dye solution, the concentration of the dye is 0.5-8 mg/ml, or 3.95 mg/ml.

16. A polyion thin film, wherein the polyion thin film is prepared by the method of claim 15.

17. A detection kit, comprising the polyion thin film of claim 16.

18. The kit of claim 17, wherein the detection kit further comprises a colorimetric card.