Claims
- 1. A method for preparing PGE.sub.1 which comprises:
- alkylating lithium cyclopentadiene with ethyl-7-bromo-heptanoate
- oxygenating the resulting alkylated cyclopentadiene to produce a mixture of hydroxycyclopentenones
- chromatographically separating the said mixture and recovering 2-(6'-carboethoxyhexyl)-4-hydroxy-cyclopenten-1-one
- reacting the said recovered hydroxy ester with an excess of dihydropyran in the presence of an acid catalyst and recovering 2-(6'-carboethoxyhexyl)-2-cyclopentene-4-tetrahydropyranoxy-1-one from the reaction mixture
- reacting the said ether with the oxygenated vinyl lithium derivative obtained from the reaction of lithium powder and 3-(.alpha.-ethoxy)-ethoxy-1-iodo-trans-1-octene in the presence of tri-n-butylphosphine-copper iodide complex and recovering the formed prostanoic acid derivative from the reaction mixture
- removing the tetrahydropyranyl and ethoxy-ethoxy ether groups from the said derivative
- chromatographically recovering PGE.sub.1 ethyl ester from the reaction mixture and
- exposing the said ester to the action of an esterase-producing microorganism and recovering PGE.sub.1 from the reaction mixture.
Parent Case Info
This is a division of application Ser. No. 221,058, filed Jan. 26, 1972, now U.S. Pat. No. 4,031,129, issued June 21, 1977.
Government Interests
The invention described herein was made in the course of work under a grant or award from the Department of Health, Education, and Welfare.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3840434 |
Daniels |
Oct 1974 |
|
3968141 |
Sih et al. |
Jul 1976 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
221058 |
Jan 1972 |
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