Patent Grant
11512318
The Sequence Listing submitted in text format (.txt) filed on Nov. 26, 2019, named “SequenceListinq.txt”, created on Nov. 11, 2019 (12.2 KB), is incorporated herein by reference.
The present invention relates to a method for producing fucosyllactose from a variant microorganism for producing 3-fucosyllactose using a wild Corynebacterium glutamicum strain, glucose and lactose, and more particularly to a method for producing 3-fucosyllactose using a recombinant Corynebacterium glutamicum (C. glutamicum) obtained by introducing, into Corynebacterium glutamicum, phosphomannomutase (ManB), mannose-1-phosphate guanylyltransferase (ManC), GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG), α-1,3-fucose transferase and a variant lac operon, from which lacZ is removed.
Human breast milk contains 200 or more kinds of human milk oligosaccharides (HMOs) having different structures which are present at a considerably higher concentration (5 to 15 g/L) than other mammals. Such HMO has functions essential for infants such as a prebiotic effect, the effect of inhibiting adhesion of pathogens on the intestines and the effect of modulating the immune regulation system.
Meanwhile, among HMOs, 3-fucosyllactose is reported to be a core HMO involved in a variety of biological activities. Methods of producing 3-fucosyllactose include direct extraction from breast milk and chemical or enzymatic synthesis. However, direct extraction has drawbacks of limited breast milk supply and low productivity and chemical synthesis has drawbacks of expensive substrates, low stereo-selectivity and production yield, the use of toxic organic solvents and the like. In addition, enzymatic synthesis has drawbacks in that GDP-L-fucose used as a fucose donor is very expensive and purification of fucosyltransferase is expensive.
As such, it is difficult to apply direct extraction and chemical or enzymatic production to the mass-production of fucosyllactose. However, biotechnological production using microorganisms is in the spotlight as a method for producing 3-fucosyllactose having the potential to be developed into a functional food and pharmaceutical material since it is capable of producing a large amount of fucosyllactose from a cheap substrate through a simple process.
Meanwhile, conventional methods for producing 3-fucosyllactose using microorganisms are mostly production techniques using recombinant E. coli. E. coli, which is used for experiments, is strongly considered to be a harmful bacterium by consumers although it is not actually a pathogen and incurs high costs of separation and purification because the cell membrane component can act as an endotoxin. For this reason, it is difficult to use E. coli as a host cell to produce 3-fucosyllactose.
Korean Patent No. 10-1544184 (Registration Date: Aug. 21, 2015) relates to a mutant microorganism for producing 2′-fucosyllactose and a method for producing 2′-fucosyllactose using the same, and discloses a variant microorganism obtained by introducing or amplifying one or more genes selected from the group consisting of a gene introduced with an operon, in which lacZ is modified or removed, and a gene encoding G6PDH (glucose-6-phosphate dehydrogenase) and GSK (guanosine-inosine kinase), and a method for producing 2′-fucosyllactose using the same.
Korean Patent No. 10-1648352 (Registration Date: Aug. 9, 2016) discloses a recombinant E. coli producing fucosyllactose in which at least one of genes encoding fucose isomerase (FucI), fuculose kinase (fucK) and fuculose 1-phosphate aldolase (FucA), which are fucose metabolic enzymes, is disrupted, and including, instead of a wild-type lac operon, a lac operon that includes a lacZ gene, a wild-type lacY gene and a wild-type lacA gene, encoding beta galactosidase having activity lower than wild beta galactosidase, or a lac operon that is completely free of a wild-type lacZ gene and includes only a wild-type lacY gene and a wild-type lacA gene, and a method for producing fucosyllactose using the same, which can produce 2- or 3-fucosyllactose with high productivity.
Therefore, the present invention has been made in view of the above problems, and it is one object of the present invention to develop and provide a method for producing 3-fucosyllactose at a high concentration, high yield and high productivity while using, as a host cell that produces fucosyllactose, which is a food and/or medicinal material, Corynebacterium glutamicum, which is safer than Escherichia coli.
In accordance with the present invention, the above and other objects can be accomplished by the provision of recombinant Corynebacterium glutamicum which is transformed to express α-1,3-fucosyltransferase, is transformed to express GDP-D-mannose-4,6-dehydratase, is transformed to express GDP-L-fucose synthase, and is transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
In the recombinant Corynebacterium glutamicum of the present invention, the α-1,3-fucosyltransferase is preferably encoded by an azoT gene. In this case, the azoT gene preferably has a nucleic acid sequence set forth in SEQ ID NO: 5.
In the recombinant Corynebacterium glutamicum of the present invention, the recombinant Corynebacterium glutamicum is preferably transformed to overexpress phosphomannomutase and is transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase.
In accordance with another aspect, provided is a method of producing 3-fucosyllactose including culturing, in a medium supplemented with lactose, recombinant Corynebacterium glutamicum, which is transformed to express α-1,3-fucosyltransferase, is transformed to express GDP-D-mannose-4,6-dehydratase, is transformed to express GDP-L-fucose synthase, and is transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
In the method of producing 3-fucosyllactose, the medium may further include glucose. In this case, the production of the 3-fucosyllactose may be carried out by batch culture or fed-batch culture including the further supply of glucose or lactose.
According to the present invention, it is possible to produce 3-fucosyllactose using a Corynebacterium glutamicum strain, which is a GRAS strain. 3-fucosyllactose can be produced more safely than conventional Escherichia coli. In addition, using the Corynebacterium glutamicum strain according to the present invention, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
The above and other objects, features and other advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which:
In one aspect, the present invention is directed to recombinant Corynebacterium glutamicum which is transformed to express α-1,3-fucosyltransferase, is transformed to express GDP-D-mannose-4,6-dehydratase, is transformed to express GDP-L-fucose synthase, and is transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
The prevent inventors filed a patent application entitled “Method for producing 3-fucosyllactose using Escherichia coli” as Korean Patent Application No. 10-2016-0012803 (Feb. 2, 2016). However, it has been frequently indicated that producing 3-fucosyllactose for functional food additive applications using Escherichia coli may cause problems due to various safety associated with Escherichia coli. Accordingly, in accordance with the present invention, there is an attempt to produce 3-fucosyllactose using an alternative strain free of food safety problems.
The present invention adopts Corynebacterium glutamicum as a host cell producing 3-fucosyllactose. Unlike conventionally used Escherichia coli, this strain is considered to be a GRAS (generally recognized as safe) strain which does not produce endotoxins and is widely used for industrially producing amino acids and nucleic acids as food additives. Accordingly, Corynebacterium glutamicum is considered to be a stain suitable for the production of food and medicinal materials while advantageously eliminating customer fears about safety.
However, since Escherichia coli and Corynebacterium glutamicum have inherently different genetic properties, strategies different from those for Escherichia coli should be applied to Corynebacterium glutamicum. Escherichia coli and Corynebacterium glutamicum are the same in that external α-1,3-fucosyltransferase should be basically incorporated in order to produce 3-fucosyllactose. However, Corynebacterium glutamicum further requires the incorporation of GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (this enzyme is called “GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase” and is also simply referred to as “WcaG”, and a gene encoding this enzyme is particularly referred to as “WcaG”), and lactose permease (Lacy). That is, Escherichia coli has genes encoding GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (Lacy), but the Corynebacterium glutamicum strain has no genes encoding these enzymes, so it is necessary to incorporate such genes from an external source and express the same.
In this case, the gene encoding α-1,3-fucosyltransferase is preferably azoT derived from Azospirillum brasilens. In addition, genes encoding GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY) are preferably derived from Escherichia coli.
Meanwhile, the recombinant Corynebacterium glutamicum of the present invention is preferably transformed to overexpress phosphomannomutase, and is transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase. Corynebacterium glutamicum possesses genes encoding phosphomannomutase (ManB) and GTP-mannose-1-phosphate guanylyltransferase (ManC), and can thus express the same. Therefore, there may be no need to incorporate genes encoding the enzymes, but the enzymes should be overexpressed for mass-production. For this reason, the present invention requires transformation of Corynebacterium glutamicum in order to overexpress the two enzymes.
Meanwhile, the actions of the enzymes can be understood from
Meanwhile, the term “expression” as used herein means incorporation and expression of external genes into strains in order to intentionally express enzymes that cannot be inherently expressed by the Corynebacterium glutamicum strain according to the present invention, and the term “overexpression” as used herein means overexpression that is induced by artificially increasing the amount of expressed enzyme in order to increase expression for mass-production, although the Corynebacterium glutamicum strain according to the present invention has genes encoding the corresponding enzyme and therefore can self-express the same.
Meanwhile, the present inventors can mass-produce 3-fucosyllactose, which is a breast milk oligosaccharide, in Corynebacterium glutamicum (C. glutamicum) through the transformation strategy described above.
Meanwhile, according to the present invention, genes encoding α-1,3-fucosyltransferase are, for example, encoded by azoT genes, wherein the azoT genes have the nucleic acid sequence set forth in SEQ ID NO: 5. In order to produce α-1,3-fucosyllactose, α-1,3-fucosyltransferase, which performs the production reaction of α-1,3-fucosyllactose using GDP-L-fucose and lactose as substrates is required (see
Meanwhile, in another aspect, the present invention is directed to a method for producing 3-fucosyllactose including culturing the recombinant Corynebacterium glutamicum of the present invention in a medium supplemented with lactose. When the recombinant Corynebacterium glutamicum strain according to the present invention is used, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
Meanwhile, regarding the method for producing 3-fucosyllactose according to the present invention, the medium preferably further includes glucose. By adding glucose to the medium, the growth of a strain can be facilitated, and 3-fucosyllactose can thus be produced at higher productivity.
Meanwhile, the method for producing 3-fucosyllactose according to the present invention is preferably carried out through batch culture or fed-batch culture that involves further supplying lactose. The detailed technologies associated with batch culture and fed-batch culture are well-known in the art and are not described herein.
Meanwhile, lactose permease was introduced into the strain Corynebacterium glutamicum of the present invention in order to incorporate lactose, which is the substrate for 3′-FL production, into strain cells. That is, in order to produce 3′-FL using Corynebacterium glutamicum, the strain of the present invention should be transformed with lactose permease capable of introducing lactose into strain cells. Accordingly, the strain of the present invention is transformed with this enzyme.
In this regard, the activity of lactose permease is usually inhibited by so-called “glucose repression” in the presence of glucose. As a result, the influx of lactose in the presence of glucose does not occur and thus 3-FL is not produced.
However, in the Corynebacterium glutamicum used in the present invention as a host strain for the production of 3′-FL, glucose repression does not occur and 3′-FL production is possible based on the inflow of lactose, even in the presence of glucose. As a result, the productivity of 3′-FL can be maximized.
Meanwhile, it can be identified from the following experiment of the present invention that the method for producing 3′-FL using the Corynebacterium glutamicum according to the present invention is based on a production pattern of non-growth-associated product formation.
The non-growth-associated product formation, in which the production of metabolites is independent of the growth of the host strain, does not further require the growth of the host strain for the production of metabolites, thus having an advantage of mass-producing large quantities of metabolites within a short time by culturing a large amount of host strain and incorporating a substrate therein. In addition, the non-growth-associated product formation has an advantage of maximizing productivity because the host strain used in the culture can be used repeatedly. Therefore, the method for producing 3′-FL established using Corynebacterium glutamicum according to the present invention is a method capable of maximizing the production of 3′-FL.
Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the examples, and includes variations and technical concepts equivalent thereto.
Escherichia coli TOP10 was used for cloning to produce plasmids and Corynebacterium glutamicum (C. glutamicum) ATCC 13032 is used to produce 3-fucosyllactose (3′-FL). Plasmids for expressing PVBCL expressing manB, manC and lacYA gene clusters developed in previous studies were used. In addition, a vector was constructed to express α-1,3-fucosyltransferase (azoT) in the plasmids for expressing pEGW expressing Gmd and WcaG genes. At this time, the origin of α-1,3-fucosetransferase (azoT) is Azospirillum brasilense ATCC 29145, and α-1,3-fucosetransferase (azoT) was inserted into the pEGW vector using a restriction enzyme of Sac1.
Meanwhile, gene sequences of ManB, ManC, Gmd, WcaG, lacYA and α-1,3-fucosetransferase (azoT), strains, plasmids and oligonucleotides used above are shown in Tables 1 to 3 below.
E. coli
C. glutamicum
(1) Culture Conditions and Methods
Seed culture was carried out using a test tube containing 5 mL of BHI (brain heart infusion) medium supplemented with appropriate antibiotics (kanamycin 25 μg/mL and tetracycline 5 μg/mL) at a temperature of 30° C. and a constant stirring rate of 250 rpm for 12 hours.
Batch culture was carried out at 30° C. in a 250 mL flask containing 100 mL ((NH4)2SO4 20 g/L, urea 5 g/L, KH2PO4 1 g/L, K2HPO4 1 g/L, MgSO4 0.25 g/L, MOPS 42 g/L, CaCl2) 10 mg/L, Biotin 0.2 mg/L, Protocatechuic acid 30 mg/L, FeSO47H2O 10 mg/L, MnSO4H2O 10 mg/L, ZnSO47H2O 1 mg/L, CuSO4 0.2 mg/L, NiCl26H2O 0.02 mg/L, pH 7.0). The stirring rate was maintained at 250 rpm during culture. In case of batch culture, IPTG (isopropyl-ß-D-thiogalactopyranoside) and lactose were added such that final concentrations were adjusted to 1.0 mM and 10 g/L, respectively, when the optical density (OD600) reached 0.8.
The fed-batch culture for high-concentration cell culture was carried out in a 2.5 L bioreactor (Kobiotech, Incheon, Korea) containing 1.0 L of a minimum medium supplemented with 40 g/L of glucose and appropriate antibiotics (25 μg/mL of kanamycin and 5 μg/mL of tetracycline).
After the glucose added at an initial stage was completely consumed, a feeding solution including 800 g/L of glucose was supplied at a rate of 5.7 g/L/h by a continuous feeding method. At the same time, IPTG and lactose were added such that final concentrations were adjusted to 1.0 mM and 10 g/L, respectively, in order to induce expression of tac-promotor-mediated genes and thereby produce 3-fucosyllactose.
When pH of the medium was lower than a set point during fermentation, 28% NH4OH was automatically supplied, and when the pH was higher than the set point, 2N HCl was added, so that the pH could be maintained within a predetermined range from 6.98 to 7.02. The pH of the medium was measured in real time using a pH electrode (Mettler Toledo, USA). The stirring rate and aeration rate were maintained at 1,000 rpm and 2 vvm to prevent lack of oxygen.
(2) Determination of Concentrations of Cells and Metabolites
The dried cell weight was determined by multiplying the optical density (OD) by a pre-measured transmutation constant of 0.3. The optical density (OD) was adjusted to the range of 0.1 to 0.5 by diluting a sample to an appropriate level, and the absorbance at 600 nm was measured using a spectrophotometer (Ultrospec 2000, Amersham Pharmacia Biotech, USA).
The concentrations of 3-fucosyllactose, lactose, lactate, glucose and acetic acid were measured using a high-performance liquid chromatography (HPLC) device (Agilent 1100LC, USA) equipped with a carbohydrate analysis column (Rezex ROA-organic acid, Phenomenex, USA) and a refractive index (RI) detector. 20 μl of the culture medium, diluted 10×, was analyzed using a column pre-heated to 60° C. 5 mM of a H2SO4 solution was used as a mobile phase at a flow rate of 0.6 mL/min.
(3) Production of 3′-Fucosylactose Through Batch Culture
Recombinant Corynebacterium glutamicum introduced with a lac operon (lacYA) free of ManB, ManC, Gmd, WcaG, azoT and lacZ was batch-cultured in each flask in order to investigate 3-fucosyllactose productivity and fermentation characteristics. IPTG and lactose were added such that final concentrations were adjusted to 1.0 mM and 10 g/L, respectively, when optical density (OD600) reached 0.8.
As a result of the flask batch culture, 390 mg/L of 3-fucosyllactose was produced. The yield of 2′-fucosyllactose relative to lactose was 0.32 (moles of 2′-fucosyllactose/moles of lactose), and the productivity was 5.49 mg/L/h (see
Corynebacterium glutamicum (C. glutamicum) pVBCL + pEGWA
aConcentration of 3′-fucosyllactose of lactose is calculated from only that present in medium.
(4) Production of 3-Fucosyllactose Through Fed-Batch Culture
In order to produce high-concentration 3-fucosyllactose through high-concentration cell culture, fed-batch culture was conducted in a 2.5 L fermenter using recombinant Corynebacterium glutamicum (C. glutamicum) introduced with pVBCL and pEGWA plasmids.
In order to maintain cell growth from when 40 g/L glucose supplied at an initial stage was completely consumed, a feeding solution started to be supplied at a rate of 5.7 g/L/h by a continuous feeding method. At the same time, IPTG and lactose were added to induce the production of 3-fucosyllactose.
As a result of the experiment, acetic acid was found not to be produced at all during fermentation, and the dried cell weight reached 48.9 g/L due to metabolism of glucose. In addition, the maximum concentration of 3-fucosyllactose was 3.6 g/L, the yield (ratio of moles of 3-fucosyllactose to moles of lactose) was 0.17 mole/mole, and the productivity was 0.03 g/L/h (
The results of fed-batch culture to produce 3-fucosyllactose are shown in the following Table 5, and
Corynebacterium glutamicum (C. glutamicum) pVBCL + pEGWT
aConcentration of 3′-fucosyllactose of lactose is calculated from only that present in medium.
b3-FL productivity was calculated after IPTG induction.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2017-0051871 | Apr 2017 | KR | national |
| 10-2018-0045845 | Apr 2018 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2018/004597 | 4/20/2018 | WO |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2018/194411 | 10/25/2018 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 6875591 | Koizumi et al. | Apr 2005 | B1 |
| Number | Date | Country |
|---|---|---|
| 3315610 | May 2018 | EP |
| 2014-506474 | Mar 2014 | JP |
| 10-2012-0122098 | Nov 2012 | KR |
| 10-1544184 | Aug 2015 | KR |
| 10-1648352 | Aug 2016 | KR |
| 1020160012803 | Aug 2017 | KR |
| WO2015150328 | Aug 2015 | WO |
| 2016040531 | Mar 2016 | WO |
| 2017188684 | Nov 2017 | WO |
| Entry |
|---|
| Chin et al. Bioprocess Biosyst Eng. Jun. 2013;36(6):pp. 749-756. |
| EP3315610 PDF 2016 pp. 1-41. |
| NCBI Genbank Accession No. CP007794.1, Jul. 26, 2016. |
| Chin et al., “Metabolic engineering of Corynebacterium glutamicum to produce GDP-1-fucose from glucose and mannose”, Bioprocess Biosyst Eng, 2013, vol. 36, pp. 749-756. |
| GenBank Accession No. CR626927; Bacteroides fragilis NCTC P343, complete genome, Cerdeno-Tarraga, A.M.,et al. “Extensive DNA inversions B. fragilis genome control variable gene expression”; Science 307 (5714), ( 2 pages total) 2005, pp. 1463-1465, 343, complete genome; Genbank Accession No. CR626927.1; (2 pages total). |
| Number | Date | Country | |
|---|---|---|---|
| 20200080095 A1 | Mar 2020 | US |
