Claims
- 1. A method for producing a catalytic monoclonal antibody which is capable of catalytically increasing the rate of a chemical reaction wherein at least one reactant is converted to at least one product comprising the steps of:
- (a) generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of:
- (i) the reactant;
- (ii) the reactant bound to a peptide or other carrier molecule;
- (iii) a reaction intermediate;
- (iv) an analog of the reactant;
- (v) an analog of the product in which the monoclonal antibody so generated is capable of binding to the reactant or a reaction intermediate; and
- (vi) an analog of a reaction intermediate; and
- (b) screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction.
- 2. A method as recited in claim 1 wherein said antigen is bound to a carrier molecule.
- 3. A method as recited in claim 1 wherein said analog is a compound which structurally resembles said reactant, reaction intermediate or reaction product to such a degree that an antibody raised against said analog participates in an immunological reaction but does not necessarily catalyze a reaction of said analog.
- 4. A method as recited in claim 3 wherein said analog is an isomer or homolog of said reactant, reaction intermediate or product.
- 5. A method as recited in claim 1 wherein said antigen is an analog of the reactant.
- 6. A method as recited in claim 1 wherein said reaction is one which is also capable of being catalyzed by a cofactor or by an enzyme and a cofactor or enzyme is present in said reaction.
- 7. A method as recited in claim 1 wherein the monoclonal antibody identified in step (b) is produced in quantity by culturing a plurality of hybridoma cells each of which produces said monoclonal antibody.
- 8. A method for producing catalytic monoclonal antibodies which are capable of catalytically increasing the rate of a chemical reaction wherein at least one reactant is converted to at least one product comprising the steps of:
- (a) generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of:
- (i) the reactant;
- (ii) the reactant bound to a peptide or other carrier molecule;
- (iii) a reaction intermediate;
- (iv) an analog of the reactant;
- (v) an analog of the product in which the monoclonal antibody so generated is capable of binding to the reactant or a reaction intermediate; and
- (vi) an analog of a reaction intermediate;
- (b) screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction; and
- (c) producing a quantity of the monoclonal antibody identified in step (b).
- 9. A method for producing a catalytic monoclonal antibody which is capable of catalytically increasing the rate of a chemical reaction wherein at least one reactant is converted to at least one product comprising the steps of:
- (a) immunizing an animal with an antigen selected from the group consisting of
- (i) the reactant,
- (ii) the reactant bound to a peptide or other carrier molecule,
- (iii) a reaction intermediate,
- (iv) an analog of the reactant,
- (v) an analog of the product in which the monoclonal antibody so generated is capable of binding to the reactant or a reaction intermediate, and
- (vi) an analog of a reaction intermediate, thereby generating antibody-producing lymphocytes in said animal;
- (b) removing said antibody-producing lymphocytes from said animal;
- (c) fusing said antibody-producing lymphocytes with myeloma cells and thereby producing a plurality of hybridoma cells each producing monoclonal antibodies; and
- (d) screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction.
- 10. A method as recited in claim 9 wherein said analog is a compound which structurally resembles said reactant, said product or said reaction intermediate to such a degree that an antibody raised against said analog participates in an immunological reaction but does not necessarily catalyze a reaction of said analog.
- 11. A method as claimed in claim 9 wherein said antigen is bound to a carrier molecule.
- 12. A method as claimed in claim 10 wherein said analog is a isomer or homolog of said reactant, product or reaction intermediate.
- 13. A method for producing catalytic monoclonal antibodies which are capable of catalytically increasing the rate of a chemical reaction which is known to be catalyzed by an enzyme and wherein at least one reactant is converted to at least one product comprising the steps of:
- (a) generating a plurality of monoclonal antibodies to said enzyme;
- (b) screening said plurality of monoclonal antibodies to identify a first monoclonal antibody which inhibits binding of the reactant to the enzyme;
- (c) recovering the said first monoclonal antibody;
- (d) generating a plurality of anti-idiotype monoclonal antibodies to the said first antibody recovered in step (c);
- (e) screening said plurality of anti-idiotype monoclonal antibodies generated in step (d) to identify a second monoclonal antibody which binds the reactant and catalytically increases the rate of the reaction; and
- (f) producing a quantity of the monoclonal antibody identified in step (e) by culturing a plurality of hybridima cells, each of the which produces said monoclonal antibody.
Parent Case Info
This application is a division of application Ser. No. 674,253, now U.S. Pat. No. 4,888,281, filed Nov. 27, 1984, which is a continuation-in-part of application Ser. No. 556,016, filed Nov. 29, 1983, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4888281 |
Schochekman et al. |
Dec 1989 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
674253 |
Nov 1984 |
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
556016 |
Nov 1983 |
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