Claims
- 1. A method for producing cholesterol oxidase comprising:
- growing a cholesterol oxidase-producing microorganism in a medium comprising a cholesterol oxidase inducer, from about 1.0 to about 5.0 g./liter of a nonionic surfactant which is non-toxic to the microorganism, and at least about 10 g./liter of yeast extract, thus producing cholesterol oxidase; and
- recovering the cholesterol oxidase.
- 2. A method for producing cholesterol oxidase comprising:
- growing a cholesterol oxidase-producing microorganism of the order Actinomycetales in a medium comprising a cholesterol oxidase inducer, from about 1.0 to about 5.0 g./liter of a nonionic surfactant which is non-toxic to the microorganism, and at least about 10 g./liter of yeast extract, thus producing cholesterol oxidase; and
- recovering the cholesterol oxidase.
- 3. A method for producing cholesterol oxidase comprising:
- growing a strain of microorganism selected fromm the group consisting of the families Corynebacteriaceae, Mycobacteriaceae, Norcardiaceae and Streptomycetaceae in a medium comprising a cholesterol oxidase inducer, from about 1.0 to about 5.0 g./liter of a nonionic surfactant which is non-toxic to the bacterium, and at least about 10 g./liter of yeast extract, thus producing cholesterol oxidase; and
- recovering the cholesterol oxidase.
- 4. The method for producing cholesterol oxidase as described in claim 3 wherein said inducer is cholesterol, a cholesterol ester or a 3-.beta.-hydroxysterol.
- 5. The method for producing cholesterol oxidase as described in claim 3 wherein said inducer is present in a concentration of from about 1.0 to about 10 g./liter and said yeast extract is present in a concentration of from about 10 to about 30 g./liter.
- 6. A method for producing cholesterol oxidase comprising:
- growing a strain of microorganism selected from the group consising of NRRL 5635, NRRL 5636, NRRL 5767, NRRL 5768, ATCC 4277, ATCC 14349, and ATCC 17985 in a medium comprising from about 1.0 to about 10 g./liter of an inducer selected from the group consisting of cholesterol, cholest-4-en-3-one, .beta.-sitosterol, 5-.alpha.-cholestan-3-.beta.-ol, cholesteryl linoleate, cholesteryl oleate, cholesteryl formate, cholesteryl propionate and cholesterol linolenate, from about 1.0 to about 5.0 g./liter of a nonionic surfactant having a polyoxyethylene or polyglycidol hydrophilic moiety and a lipophilic moiety comprising at least 9 carbon atoms, and from about 10 to about 30 g./liter of yeast extract, thus producing cholesterol oxidase; and
- recovering the cholesterol oxidase.
- 7. The method for producing cholesterol oxidase as described in claim 6 wherein said nonionic surfactant comprises a hydrophilic moiety having at least about 20 units of polyoxyethylene and a lipophilic moiety having a fatty acid chain containing at least 16 carbon atoms.
- 8. The method for producing cholesterol oxidase as described in claim 6 wherein said inducer is selected from the group consisting of cholesterol, .beta.-sitosterol, cholesteryl oleate, cholesteryl linoleate and cholesteryl propionate, and said nonionic surfactant comprises a hydrophilic moiety consisting of 1 unit of sorbitan and 20 units of polyoxyethylene and a lipophilic moiety consisting of 1 unit of palmitic acid.
- 9. A method for producing cholesterol oxidase comprising:
- growing a strain or microorganism selected from the group consisting of NRRL 5635, NRRL 5636, NRRL 5767, NRRL 5768, ATCC 4277, ATCC 14349, and ATCC 17895 in a medium comprising from about 2.0 to about 5.0 g./liter of an inducer selected from the group consisting of cholesterol, .beta.-sitosterol, cholesteryl oleate, cholesteryl linoleate and cholesteryl propionate, from about 1.0 to about 5.0 g./liter of a nonionic surfactant comprising a hydrophilic moiety consisting of 1 unit of sorbitan and 20 units of polyoxyethylene and a lipophilic moiety consisting of 1 unit of palmitic acid, and from about 10 to about 30 g./liter of yeast extract, thus producing cholesterol oxidase; and
- recovering the cholesterol oxidase.
- 10. The method for producing cholesterol oxidase as described in claim 9 wherein said inducer is cholesterol or .beta.-sitosterol and said yeast extract is present in a concentration of about 20 g./liter.
Parent Case Info
This application is a continuation-in-part of U.S. patent application Ser. No. 639,690, filed Dec. 11, 1975.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3776816 |
Terada et al. |
Dec 1973 |
|
3884764 |
Goodhue et al. |
May 1975 |
|
3909359 |
Goodhue et al. |
Sep 1975 |
|
Foreign Referenced Citations (3)
Number |
Date |
Country |
2,246,695 |
Mar 1973 |
DT |
733,259 |
Apr 1973 |
ZA |
1,385,319 |
Feb 1975 |
UK |
Non-Patent Literature Citations (2)
Entry |
Voets et al., "Microbial Degradation of Cholesterol," Chemical Abstracts, vol. 81, No. 1, p. 96 (1974) abs. No. 1140e. |
Reese et al., "Surfactants as Stimulants of Enzyme Production by Microorganisms," Applied Microbiology,vol. 17, No. 2 (1969), pp. 242-245. |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
639690 |
Dec 1975 |
|