Claims
- 1. A method for producing and isolating creatinase which comprises culturing a creatinase producing microorganism Bacillus sp. B-0618, Firm-P No. 4049, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography from the sarcosine oxidase- and creatinase-containing solution thus produced to obtain a creatinase eluted fraction and collecting creatinase therefrom.
- 2. A method according to claim 1 wherein the anion exchange chromatography medium is diethylaminoethyl cellulose and the creatinase eluted fraction is eluted with 0.3 M KCl solution in concentration gradient of KCl 0.1 M to 0.5 M.
- 3. A method according to claim 1 wherein the anion exchange chromatography medium is diethylaminoethyl cellulose and the creatinase eluted fraction is eluted with 0.2-0.5 M KCl solution in concentration gradient of KCl 0.35-0.37 M.
Priority Claims (1)
Number |
Date |
Country |
Kind |
54-85260 |
Jul 1979 |
JPX |
|
Parent Case Info
This application is a continuation-in-part application of application Ser. No. 338,607 filed on Jan. 11, 1982, abandoned which is a continuation-in-part application of Ser. No. 158,800, filed June 12, 1980, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3907644 |
Mollering et al. |
Sep 1975 |
|
4039384 |
Suzuki et al. |
Aug 1977 |
|
4216292 |
Ikuta et al. |
Aug 1980 |
|
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
338607 |
Jan 1982 |
|
Parent |
158800 |
Jun 1980 |
|