Claims
- 1. A method for producing L-glutamic acid, which comprises cultivating an L-glutamic acid-producing microorganism which requires oleic acid but does not require biotin for growth in a culture medium containing an oleic acid compound and a biotin compound of no less than 100 .mu.g/liter as biotin, with carbohydrate and acetic acid as carbon sources being maintained in a weight ratio of about 70:30 through about 50:50, said microorganisms being from the group consisting of Brevibacterium thiogenitalis D248, Ferm BP-433, Brevibacterium flavum BN-11, Ferm BP-435, and Corynebacterium glutamicum No. 534-MS-023, Ferm BP-434.
- 2. A method as claimed in claim 1, wherein the carbohydrate is glucose.
- 3. A method as claimed in claim 1, wherein the carbohydrate is sucrose.
- 4. A method as claimed in claim 1, wherein the carbohydrate is saccharified starch of sweet potato.
- 5. A method as claimed in claim 1, wherein the carbohydrate is molasses.
- 6. A method as claimed in claim 1, wherein the carbohydrate and acetic acid are maintained in the culture medium in a weight ratio of about 60:40.
- 7. A method as claimed in claim 1, wherein the amount of the oleic acid compound contained in the medium is not less than about 60 mg/liter.
Priority Claims (1)
Number |
Date |
Country |
Kind |
58-46712 |
Mar 1983 |
JPX |
|
Parent Case Info
This application is a continuation, of now abandoned application Ser. No. 580,636, filed Feb. 16, 1984.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4368266 |
Tosaka et al. |
Jan 1983 |
|
Foreign Referenced Citations (2)
Number |
Date |
Country |
0114393 |
Sep 1980 |
JPX |
1224893 |
Mar 1971 |
GBX |
Continuations (1)
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Number |
Date |
Country |
Parent |
580636 |
Feb 1984 |
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