Claims
- 1. A method for the production of native human immunodeficiency virus gp160 comprising:
- infecting cells from a HUT78 T-cell line with HTLV-III.sub.451 ;
- selecting infected HUT78 cells that produce native gp160;
- incubating said gp160 producing cell line in serum-free medium under conditions that promote cell growth; and
- isolating native gp160 from said medium.
- 2. In a process for the production of envelope proteins from human immunodeficiency virus wherein a T-cell line is infected with said virus, incubated in a medium under conditions that promote cell growth, and glycoproteins are secreted into said medium, the improvement which comprises:
- incubating cells from a gp160 producing 6D5.sub.451 infected T-cell line in serum-free medium, and
- isolating from said medium a 160 kD protein which corresponds to native HIV envelope glycoprotein gp160.
Parent Case Info
This is a continuation of Ser. No. 232,859, filed Aug. 16, 1988, now abandoned.
Government Interests
The invention described herein was made during the course of work performed under National Cancer Institute, Department of Health and Human Services Contract No. NO1-CP-67694.
Non-Patent Literature Citations (3)
Entry |
Kalyanaraman et al, Aids Research and Human Retroviruses 4:319-329 (88). |
Getchell et al, J. Clinical Microbiology 23:737-741 (86). |
Pyle et al, J. of Virology 62:2258-2264 (88). |
Continuations (1)
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Number |
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Parent |
232859 |
Aug 1988 |
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