Patent Application
20070292541
The present invention is explained in more detail by the following examples, yet without being restricted thereto.
The aim of the examinations was to selectively introduce chemically defined compounds of vitamins from the B group into plant organisms and to convert them into biologically active compounds. Germinative, edible plant seeds were particularly suitable as plant enrichment agents. Edible germ buds from cereals and other plant seeds are considered particularly valuable according to current findings in nutrition research. They impress by a better protein quality as compared to non-germinated seeds, by a content pattern of polyunsaturated fatty acids of higher quality, but primarily by a better bioavailability of the vitamins, mineral substances and trace elements contained. The aim of the tests was to selectively enrich germinative seeds with vitamins of the B complex by enrichment via standardized nutrient solutions, in order to obtain germ buds with high contents above average of biologically active vitamins of the B complex, yet, at the same time, also obtaining standardized contents thereof.
In order to examine the basic absorbency of different edible germ buds for B vitamins, standardized aqueous solutions of niacine and/or cobalamin were added to germinative wheat, buckwheat and quinoa seeds. This was done in the following way:
Step 1: 100 g seeds (wheat, buckwheat, quinoa) each were prewashed with double-distilled water.
Step 2: 100 g of the pre-washed seed type each were steeped in the respective vitamin nutrient solution:
wheat for 12 hours
buckwheat for 16 hours
quinoa for 1 hour
The amounts of the respective steeping-nutrient solution were 500 ml each.
Step 3: After the predetermined steeping time in the vitamin-nutrient solutions, the pre-germinated seeds were filtered by means of a filter and pre-germinated for over 12 hours at room temperature.
Step 4: Each single sample was sprinkled with 25 ml freshly produced vitamin-nutrient solution once a day for 4 days.
Step 5: After 96 hours (4 days) germination duration, the germ buds were washed 3× with 800 ml double-distilled water each, in order to completely remove the vitamin traces adhering to the surface. Then, the samples were dried for 10 hours in the drying chamber at 70 degree Celsius.
The following Table 1 indicates the vitamin concentrations of the niacine and cobalamin nutrient solutions:
The following Table 2 indicates the niacine contents in mg/100 g dried germ bud.
W(0), B(0), Q(0) refer to non-germinated wheat, buckwheat and quinoa. W(zdw), B(zdw), Q(zdw) refer to the respective kinds of germ buds which have been germinated according to the same method as the test germ buds, yet not in vitamin-containing nutrient solutions but in double-distilled water.
The denotations (Conc.1) to (Conc.6) symbolize the respective niacine concentrations of the nutrient solutions, in which the respective seeds have been germinated.
The detection of the individual vitamin contents was done by Institut Kuhlmann, Hedwig-Laudien-Ring 3, D-67061 Ludwigshafen, according to the following analysis method:
After the pretests with the above-indicated 3 seed types had gone positively, the quinoa-seeds were caused to germinate according to a method with a complex vitamin-B nutrient solution of the following composition (Table 4): Vitamin content in 1 liter aqueous nutrient solution:
The quinoa germing buds obtained after washing, germination and drying according to the above-illustrated method had the following vitamin contents:
In a further method step it has been examined whether, depending on the duration of germination, a part of the vitamin absorbed during steeping from the steeping-soaking solution is converted into organically bound forms during the germination process. To this end, 200 g germinative quinoa seeds were soaked for 6 hours in 320 ml nutrient solution of the following composition:
Vitamins dissolved in 1 l water:
In this context, after 30 hours of germination duration and subsequent thorough washing according to the microbiological VitaFast® method of R-Biopharm GmbH/Institut für Produktqualität Teltowkanalstraβe 2, D-12047 Berlin, the following vitamin values have been analyzed
After 45 hours of germination duration and subsequent thorough washing a significant increase in the portions of organically bound vitamins occurred, employing the same analysis method:
The Vitafast® analysis method was conducted according to Lindeke (HYGIENE Report 2 (2006):4-6).
| Number | Date | Country | Kind |
|---|---|---|---|
| A 1029/2006 | Jun 2006 | AT | national |
