Claims
- 1. A process for purifying acarbose, comprising the steps of:
1) acidifying a fermentation broth containing an acarbose; 2) removing particulates from the fermentation broth; 3) adsorbing the acarbose on a cation-exchanger in the presence of an anion of a weak acid; 4) eluting the acarbose from the cation-exchanger with at least one of a sodium chloride solution and a salt solution; 5) precipitating the acarbose with a solvent; and 6) recovering the precipitated acarbose.
- 2. The process of claim 1, wherein the fermentation broth is acidified to a pH about 4 to about 6.
- 3. The process of claim 1, wherein the fermentation broth is acidified to a pH about 5.
- 4. The process of claim 1, wherein the fermentation broth is acidified with a weak acid.
- 5. The process of claim 4, wherein the weak acid is acetic acid.
- 6. The process of claim 4, wherein the weak acid is selected from the group consisting of tartaric acid, succinic acid, citric acid, formic acid, malonic acid, oxalic acid, phthalic acid, benzoic acid, phosphoric acid and the derivatives thereof.
- 7. The process of claim 1, wherein the particulates are removed with a filter.
- 8. The process of claim 1, wherein the filter is pre-coat vacuum drum.
- 9. The process of claim 1, wherein the cation-exchanger is a strong acid cation-exchanger.
- 10. The process of claim 9, wherein the strong acid cation-exchanger is a resin in acid form.
- 11. The process of claim 1, wherein the anion of a weak acid is selected from the group consisting of tartarate, succinate, citrate, acetate, formate, malonate, oxalate, phthalate, benzoate, phosphate and the derivatives thereof.
- 12. The process of claim 1, wherein the acarbose is eluted from the cation-exchanger with a sodium chloride solution.
- 13. The process of claim 12, wherein the sodium chloride solution has a concentration of about 0.002 M to about 0.03 M.
- 14. The process of claim 12, wherein the sodium chloride solution has a concentration of about 0.005 M to about 0.02 M.
- 15. The process of claim 1, wherein the acarbose is eluted from the cation-exchanger with a salt solution selected from the group consisting of sodium chloride, potassium chloride and calcium chloride.
- 16. The process of claim 1, wherein the acarbose is eluted from the cation-exchanger with a salt solution selected from the group consisting of sodium sulfate, potassium sulfate and calcium sulfate.
- 17. The process of claim 1, wherein the acarbose is eluted from the cation-exchanger with a salt solution selected from the group consisting of sodium nitrate, potassium nitrate and calcium nitrate.
- 18. The process of claim 1, wherein the acarbose is eluted from the cation-exchanger with a salt solution selected from the group consisting of sodium acetate, potassium acetate and calcium acetate.
- 19. The process of claim 1, wherein the solvent used for precipitation is selected from the group consisting of alcohols, mixture of alcohols, acetone, acetonitrile, ester of acetic acid, ester of formic acid and ester of propionic acid.
- 20. A process for purifying acarbose, comprising the steps of:
1) acidifying a fermentation broth containing an acarbose; 2) removing particulates from the fermentation broth; 3) adsorbing the acarbose on an anion-exchanger in the presence of an anion of a weak acid; 4) eluting the acarbose from the anion-exchanger; 5) adsorbing the eluted acarbose on a cation-exchanger in the presence of the anion of a weak acid; 6) eluting the acarbose from the cation-exchanger with at least one of a sodium chloride solution and a salt solution; 7) precipitating the acarbose with a solvent; and 8) recovering the precipitated acarbose.
- 21. Pure acarbose as prepared in accordance with the process of claim 1.
- 22. A pharmaceutical formulation comprising pure acarbose as prepared in accordance with the process of claim 1, wherein the acarbose has a purity of at least about 94%.
- 23. A pharmaceutical formulation comprising pure acarbose as prepared in accordance with the process of claim 1, wherein the acarbose has a purity of at least about 96%.
- 24. Pure acarbose as prepared in accordance with the process of claim 20.
- 25. A pharmaceutical formulation comprising pure acarbose as prepared in accordance with the process of claim 20, wherein the acarbose has a purity of at least about 94%.
- 26. A pharmaceutical formulation comprising pure acarbose as prepared in accordance with the process of claim 20, wherein the acarbose has a purity of at least about 96%.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This is a continuation-in-part application of the U.S. Conventional application Ser. No. 09/924,271 filed on Aug. 7, 2001 which claims the benefit of the Provisional Application Serial No. 60/223,492 filed Aug. 7, 2000, the disclosures of which are incorporated by reference in their entireties herein.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60223492 |
Aug 2000 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09924271 |
Aug 2001 |
US |
Child |
10060831 |
Jan 2002 |
US |