Claims
- 1. A method of reducing bioavailability of lead in a lead-contaminated environment comprising adding Pseudomonas aeruginosa strain CHL004 having the lead-sequestering properties of the organism identified as ATCC No. 55937 to said environment, either (1) after ascertaining that said environment contains sufficient phosphate to provide at least one stoichiometric equivalent amounts of phosphate to lead or (2) with addition of sufficient phosphate in ionic form to said environment to provide at least one stoichiometric equivalent amount of phosphate to lead.
- 2. A method of reducing lead in a lead-contaminated environmental material comprising the steps of(1) growing Pseudomonas aeruginosa strain CHL004 having the lead-sequestering properties of the organism identified as ATCC No. 55937 on a solid matrix, (2) adding the matrix prepared in step 1 along with sufficient phosphate in ionic form to provide at least a stoichiometric equivalent amount of phosphate to lead to the environmental material containing the lead, (3) allowing sufficient time for the bacteria to sequester the lead from the environmental material, then (4) separating the matrix with the bacteria sequestering the lead from the environmental material.
- 3. A method of claim 1 wherein the Pseudomonas aeruqinosa strain CHL004 is grown on a solid matrix.
- 4. A method of claim 3 wherein the matrix is activated carbon.
- 5. A method of claim 3 wherein the matrix forms a filter.
- 6. A method of claim 1 wherein said environment is lead-containing soil.
- 7. A method of claim 2 wherein the environmental material contaminated with lead is a liquid.
- 8. A method of claim 2 wherein matrix contains iron and the matrix with bacteria sequestering the heavy metal is removed by magnetic means.
Parent Case Info
This application is a 371 of PCT/US98/08279 filed Apr. 24, 1998 which claims benefit of Prov. No. 60/044,106 filed Apr. 25, 1997.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US98/08279 |
|
WO |
00 |
4/26/1999 |
4/26/1999 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO98/49280 |
11/5/1998 |
WO |
A |
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5055402 |
Greene et al. |
Oct 1991 |
|
5443729 |
Sly et al. |
Aug 1995 |
|
5512702 |
Ryan et al. |
Apr 1996 |
|
Non-Patent Literature Citations (3)
Entry |
Vesper et al. Water, Air and Soil Pollution. 1996. 86:207-219.* |
Aickin et al. Micribios Letters. 1978. 9 (34), 55-66.* |
ATCC Bacteria and Bacteriophages. Catalogue. 19 th edition. 1996, p. 277. |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/044106 |
Apr 1997 |
US |