METHOD FOR STABILIZING HEMOGLOBIN AND REAGENTS FOR PERFORMING THE SAME

Abstract
A stool resuspension solution comprising a hemoglobin stabilization reagent is provided. In some embodiments, the hemoglobin stabilization reagent may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. A method of stabilizing hemoglobin in a stool sample in the solution is also provided, as well as a sample collection device containing the solution.
Description
BACKGROUND

Tests that detect hemoglobin (Hb) in a stool specimen are commonly used for colorectal cancer screening. The common procedures utilize a sample collection device that contains buffer to stabilize hemoglobin from the time of collection until the detection assay is conducted at the lab. The ability of the buffer to properly stabilize hemoglobin is critical for accurate test results and must stabilize hemoglobin during shipment over a range of environmental conditions. Increasing the ability of the buffer to stabilize hemoglobin would, for example, allow longer shipping durations and provide more flexibility to the patient and test lab for sample processing.


SUMMARY

Provided herein, among other things, is a stool resuspension solution that comprises a hemoglobin stabilization reagent. In some embodiments, the hemoglobin stabilization reagent in the solution may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation.


The solution finds use in methods that may benefit from stabilizing hemoglobin. In some embodiments, this method may comprise combining the stool sample with the stool resuspension solution to produce a suspension, and maintaining the suspension for a period of time. In some embodiments, this method may comprise shipping the suspension to a remote location. The hemoglobin stabilization reagent in the solution stabilizes the hemoglobin, thereby allowing the hemoglobin in the sample to be more accurately measured after several days in transit.


Compositions, methods and devices that employ the stool resuspension solution are provided.


DESCRIPTION OF EXEMPLARY EMBODIMENTS

Before the present invention is described in greater detail, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.


Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.


Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, the preferred methods and materials are now described.


All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed.


It must be noted that as used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as “solely,” “only” and the like in connection with the recitation of claim elements, or use of a “negative” limitation.


As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present invention. Any recited method can be carried out in the order of events recited or in any other order which is logically possible.


A stool resuspension solution comprising one or more hemoglobin stabilization reagents is provided. In some embodiments, the one or more hemoglobin stabilization reagents may be selected from, e.g., an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin and an HRP stabilization component and, optionally, a polyvalent cation. Examples and concentrations of such reagents in the solution are set forth in the tables shown below and in the appendices.


In some embodiments, the solution may comprise an osmolyte, e.g., betaine. In these embodiments, the osmolyte (e.g., betaine) may be at a concentration in the range of 2 M to 5 M.


In some embodiments, the solution may comprise a sugar, e.g., sucrose or trehalose. In these embodiments, the sugar (e.g., sucrose or trehalose) may be at a concentration in the range of 0.1 M to 0.5 M. In either case, the solution may optionally contain a polyvalent cation, Mg2+ or Ca2+. In these embodiments, the polyvalent cation (e.g., calcium or magnesium ions) may be at a concentration in the range of 5 mM to 25 mM.


In some embodiments, the solution may comprise a polysaccharide, e.g., a substituted or unsubstituted polygalacturonic acid such as α-(1-4)-linked D-galacturonic acid. In these embodiments, the polysaccharide (e.g., the substituted or unsubstituted polygalacturonic acid) may be at a concentration in the range of 0.005% to 0.5% (e.g., 0.01% to 0.125%). In these embodiments, the solution may optionally contain a polyvalent cation. In embodiments in which the solution contains a polyvalent cation, the polyvalent cation may be at a concentration in the range of 5 mM to 25 mM. In some embodiments, the solution may comprise substituted or unsubstituted polygalacturonic acid and a multivalent cation (e.g., a calcium salt or magnesium salt) at a concentration in the range of 5 mM to 25 mM.


In some embodiments, the solution may comprise a protoporphyrin, e.g., a protoporphyrin IX or an analog thereof such as octaethylporphyrin (H2OEP) or tetraphenylporphyrin (H2TPP), complexed with a metal ion. In these embodiments, the protoporphyrin may be hemin (protoporphyrin IX containing a ferric iron (Fe3+) ion with a coordinating chloride ligand) or hematin. In other embodiments, the protoporphyrin may be protoporphyrin IX complexed with a divalent or trivalent cation (e.g., Zn2+, Cr3+, or Co3+, for example). In these embodiments, the protoporhyrin may in the solution at a concentration in the range of 0.1 μM to 100 μM (e.g., 1 μM to 10 μM). In some embodiments, the solution may comprise an HRP stabilization component selected from HRP Conjugate Stabilizer (PN 85R-102; Fitzgerald Industries), HRP Conjugate Stabilizer (PN SZ02; Surmodics) and HRP Conjugate Stabilizer (PN ab171537; Abcam). Other HRP stabilization components, e.g., AbGuard (BioRad PN: BUF052; BioRad Laboratories Inc. 2000 Alfred Nobel Dr, Hercules, Calif. 94547) can potentially be used. If the solution comprises an HRP stabilization component, then the component may be at a concentration in the range of 1% to 20%, e.g., 5% to 15% or 5% to 20%.


In any embodiment, the solution may comprise a polyvalent cation, e.g., calcium or magnesium ions. In these embodiments, the polyvalent cation may be at a concentration in the range of 5 mM to 25 mM.


In any embodiment, the solution further comprises Tris buffer (e.g., 10 mM to 50 mM Tris, pH 7.5), bovine serum albumen (e.g., 5% to 20% BSA), polysorbate 20 (e.g., 0.05% to 0.2% polysorbate 20), a preservative such as sodium azide (e.g., 0.05% to 0.2% sodium azide), a salt such as sodium chloride (e.g., 50 mM to 250 mM sodium chloride), a chelator such as ethylenediaminetetraacetic acid (e.g., 5 mM to 20 mM ethylenediaminetetraacetic acid), and an antibiotic such as gentamicin (e.g., 5 ug/mL to 50 ug/mL).


A composition comprising: (a) a stool sample and (b) the stool resuspension solution, as described above, is also provided. The stool sample may be suspended in the solution. Some components of the stool may be dissolved in the solution, where other, insoluble components, may be suspended but not dissolved in the solution. In some embodiments, the stool sample may contain hemoglobin which may be dissolved in the solution. In these embodiments, the presence of the hemoglobin stabilization reagent may increase the amount of hemoglobin in the sample after it has been subjected to a real or simulated shipping stress, as described below, as compared to an otherwise identical control sample that has not been subjected to the stress, e.g., a “T0” sample. In some embodiments, use of the hemoglobin stabilization reagent may increase the amount of hemoglobin in the sample by at least 10%, at least 20 or at least 30%, relative to a control stool sample that has been suspended in an otherwise identical solution that does not contain the hemoglobin stabilization reagent. The amount of hemoglobin in the sample may be determined by enzyme-linked immunosorbent assay (ELISA) although several other methods are known (see, generally, Haug et al, Am J. Gastroenterology 105: 682-690; Ahlquist et al, Ann Intern Med 1984; 101:297-302; Ahlquist et al, JAMA 1993 269:1262-1267; Young et al Dig Dis Sci. 2015 60: 609-622; and Harewood et al Mayo Clin. Proc. 2002 77: 23-28).


Also provided is a method of stabilizing stool hemoglobin. In some embodiments, this method may comprise combining the stool sample with the stool resuspension solution as described above, i.e., a solution comprising one or more hemoglobin stabilization reagents selected from: an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation; a protoporphyrin; and an HRP stabilization component and, optionally, a polyvalent cation, to produce a suspension; and maintaining the suspension for a period of time. Details of the identities of the reagents that can be in the solution as well as their concentrations are described above, below and in the accompanying appendices. In some embodiments, the method may comprise obtaining the stool sample from a larger mass of stool by scraping or scooping, e.g., using a stool sampling implement.


In some embodiments, the method may comprise shipping the sample to a remote location, e.g., to another town, city, state or country, for testing. In these embodiments, the sample may be waiting to be shipped, en route, or waiting to be tested at a remote location for several days, e.g., 3, 4, 5, 6, or 7 days, thereby subjecting the sample to stresses that potentially reduce the amount of hemoglobin in the sample.


Also provided is a method that comprises receiving a composition that comprises: (a) a stool sample and (b) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from, e.g., an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, and an HRP stabilization component and, optionally, a polyvalent cation. Examples and concentrations of such reagents in the solution are set forth above, below and in the accompanying appendices. In certain embodiments, this method may further comprise performing an assay on the sample after it has been received. For example, the concentration of hemoglobin in the solution may be measured, methods for performing which are described above. In addition, the amount of one or more colorectal cancer tumor markers in the sample may be tested after it has been received (see, generally, Lech et al, World J Gastroenterol. 2016 22: 1745-1755 and Scheruders et al, Curr. Treat. Options Gastroenterol. 2016 14: 152-162). These embodiments may further comprise forwarding test results to a remote location by, e.g., fax or e-mail, etc., or via the internet via a portal, so that the results can be obtained by a medical practitioner (e.g., an MD or a nurse, etc.), in advance of providing a diagnosis to the patient from which the stool sample was obtained.


Also provided is a sample collection device that comprises: (a) a sample collection container having an open end; (b) a stool resuspension solution that is within the container, where the solution comprises one or more hemoglobin stabilization reagents selected from: an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, and an HRP stabilization component and, optionally, a polyvalent cation, as described herein; and (c) a stool sampling rod comprising a distal beveled tip for scooping and/or scraping a sample of stool. In this device, the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device. In some embodiments, the sample collection container and sampling rod may connect via a screw fit. With the exception of the hemoglobin stabilization reagent, details, options and the design of an example of such a sample collection device can be found in U.S. Pat. No. 9,211,112, for example. In some embodiments, the device may comprise a) a body comprising a sample collection chamber bounded on a distal end by a penetrable seal affixed to a recessed sealing surface of the distal end of the body and bounded on a proximal end by a septum comprising an aperture, said penetrable seal being penetrable by a pipette tip or needle; b) a flexible sampling rod comprising a proximal portion and a distal portion, said sampling rod adapted to fit through and seal said aperture when said distal portion is in said sample collection chamber, wherein said distal portion comprises: i) an asymmetrical beveled tip at a distal end, said beveled tip having an apex at the circumference of the flexible sampling rod and configured to bend the flexible sampling rod away from collisions and/or to deflect collisions with an inserted pipette tip or needle; and ii) a plurality of stacked coaxial frusta of cones to form a plurality of metering ridges; and c) the stool resuspension solution described above in the sample collection chamber, as described in U.S. Pat. No. 9,211,112.


Embodiments

Embodiment 1. A composition comprising: (a) a stool sample; and (b) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation.


Embodiment 2. The composition of embodiment 1, wherein the stool sample contains hemoglobin.


Embodiment 3. The composition of embodiment 1, wherein the solution comprises betaine.


Embodiment 4. The composition of embodiment 2, wherein the betaine is at a concentration in the range of 2 M to 5 M.


Embodiment 5. The composition of embodiment 1, wherein the solution comprises sucrose or trehalose, and, optionally, a polyvalent cation.


Embodiment 6. The composition of embodiment 5, wherein the sucrose or trehalose is at a concentration in the range of 0.1 M to 0.5 M and the solution, optionally comprises, a polyvalent cation.


Embodiment 7. The composition of embodiment 1, wherein the solution comprises substituted or unsubstituted polygalacturonic acid and, optionally, a polyvalent cation.


Embodiment 8. The composition of embodiment 1 or 7, wherein the solution comprises α-(1-4)-linked D-galacturonic acid and, optionally, a polyvalent cation.


Embodiment 9. The composition of embodiment 7, wherein the substituted or unsubstituted polygalacturonic acid is at a concentration in the range of 0.005% to 0.5%.


Embodiment 10. The composition of embodiment 1, wherein the solution comprises substituted or unsubstituted polygalacturonic acid and a multivalent cation (e.g., a calcium salt or magnesium salt) at a concentration in the range of 5 mM to 25 mM.


Embodiment 11. The composition of embodiment 1, wherein the solution comprises a protoporphyrin.


Embodiment 12. The composition of embodiment 11, wherein the protoporphyrin is hemin or hematin.


Embodiment 13. The composition of embodiment 11, wherein the protoporphyrin comprises protoporphyrin IX complexed with a divalent or trivalent cation (e.g., Zn2+, Cr3+ or Co3+).


Embodiment 14. The composition of any of embodiments 11-13, wherein the protoporphyrin is at a concentration in the range of 0.1 μM to 100 μM (e.g., 1 μM to 10 μM).


Embodiment 15. The composition of embodiment 1, wherein the solution comprises an HRP stabilization component selected from HRP Conjugate Stabilizer (PN 85R-102; Fitzgerald Industries), HRP Conjugate Stabilizer (PN SZ02; Surmodics) and HRP Conjugate Stabilizer (PN ab171537; Abcam)


Embodiment 16. The composition of embodiment 15, wherein the HRP stabilization component is at a concentration in the range of 1% to 20%.


Embodiment 17. The composition of any prior embodiment, wherein the solution comprises one or more cations selected from iron, cobalt, chromium, zinc, calcium or magnesium ions.


Embodiment 18. The composition of embodiment 17, wherein the cations are at a concentration in the range of 5 mM to 25 mM.


Embodiment 19. The composition of any prior embodiment, wherein the stool sample is suspended in the solution.


Embodiment 20. The composition of any prior embodiment, wherein the solution further comprises Tris buffer, bovine serum albumen, polysorbate 20, sodium azide, sodium chloride, ethylenediaminetetraacetic acid, and gentamicin.


Embodiment 21. A method of stabilizing hemoglobin in a stool sample, comprising: combining the stool sample with a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation; to produce a suspension; and maintaining the suspension for a period of time.


Embodiment 22. The method of embodiment 17, wherein the method comprises shipping the suspension to a remote location.


Embodiment 23. A method of analyzing a stool sample, comprising: (a) receiving, from a remote location, a composition comprising: (i) a stool sample; and (ii) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation, wherein the stool sample is suspended in the solution; and (b) measuring the amount of hemoglobin in the composition.


Embodiment 24. The method of embodiment 19, further comprising measuring the amount of one or more colorectal cancer tumor markers in the sample.


Embodiment 25. A sample collection device comprising: a sample collection container having an open end; a stool resuspension solution that is within the container, comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation; and a stool sampling rod comprising a distal beveled tip for scooping and/or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device.


Embodiment 26. The sample collection device of embodiment 25, wherein the sample collection container and sampling rod connect via a screw fit.


Embodiment 27. A method of stabilizing hemoglobin in a stool sample, comprising: combining the stool sample with a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation to produce a suspension; and maintaining the suspension for a period of time.


Embodiment 28. The method of embodiment 27, wherein the method comprises shipping the suspension to a remote location.


Embodiment 29. A method of analyzing a stool sample, comprising: (a) receiving, from a remote location, a composition comprising: (i.) a stool sample; and (ii.) a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation, wherein the stool sample is suspended in the solution; and (b) measuring the amount of hemoglobin in the composition.


Embodiment 30. The method of any of embodiments 27-29, further comprising measuring the amount of one or more colorectal cancer tumor markers in the sample.


Embodiment 31. The method of any of embodiments 27-30, wherein the multivalent cation is Cr3+ or Co3+.


Embodiment 32. The method of any of embodiments 27-31, wherein the solution has a pH in the range of pH 6.5 to pH 7.4.


Embodiment 33. The method of any of embodiments 27-32, wherein the multivalent cation is Cr3+ and the pH of the solution is in the range of pH 6.9 to pH 7.4.


Embodiment 34. The method of any of embodiments 27-32, wherein the multivalent cation is Co3+ and the pH of the solution is in the range of pH 6.5 to pH 7.0.


Embodiment 35. The method of any of embodiments 27-34, wherein the protoporphyrin IX is at a concentration in the range of 0.5 uM to 10 uM.


Embodiment 36. A sample collection device comprising: a sample collection container having an open end; a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation; and a stool sampling rod comprising a distal beveled tip for scooping and/or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device.


Embodiment 37. The sample collection device of embodiment 36, wherein the sample collection container and sampling rod connect via a screw fit.


Embodiment 38. The sample collection device of embodiment 36 or 37, wherein the multivalent cation is Cr3+ or Co3+.


Embodiment 39. The sample collection device of any of embodiments 36-38, wherein the solution has a pH in the range of pH 6.5 to pH 7.4.


Embodiment 40. The sample collection device of any of embodiments 36-39, wherein the multivalent cation is Cr3+ and the pH of the solution is in the range of pH 6.9 to pH 7.4.


Embodiment 41. The sample collection device of any of embodiments 36-39, wherein the multivalent cation is Co3+ and the pH of the solution is in the range of pH 6.5 to pH 7.0.


Embodiment 42. The sample collection device of any of embodiments 16-21, wherein the protoporphyrin IX is at a concentration in the range of 0.5 uM to 10 uM.


Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claims.







EXAMPLE 1
Simulated Shipping Stress

To assess the stability of hemoglobin in stool samples, samples were exposed to simulated shipping stress conditions using a thermal cycler. Table 1 shows the time and temperature profile for a 3-Day (72 hour) shipping simulation. An ELISA based detection method is used to determine the hemoglobin concentrations of samples before and after exposure to the shipping simulation. Hemoglobin stability is calculated as a % recovery of hemoglobin after the samples have been exposed to the shipping simulation.









TABLE 1







3-Day Shipping Simulation













Duration
Total
Temp



Simulated Shipping Condition
(hrs)
time (hrs)
(° C.)







Pre-shipping hold at
24
24
22



ambient temperature






Ambient, overnight shipping
 9
33
22




 3
36
35




 4
40
22




 5
45
35




 5
50
22




 4
54
35



Post-shipping hold at
18
72
22



ambient temperature










An accelerated shipping simulation profile was created to mimic the 3-Day shipping simulation but in a shortened amount of time. Table 2 shows the time and temperature profile for an accelerated shipping simulation. Samples exposed to the accelerated shipping simulation exhibit similar levels of Hb stability as when exposed to the 3-Day shipping simulation.









TABLE 2







Accelerated Shipping Simulation












Duration
Temp



Simulated Shipping Condition
(hrs)
(° C.)







Incubation at Temperature
16
35










To assess the stability of hemoglobin in stool samples beyond 3 days, a 7-Day shipping simulation was created. Table 3 shows the time and temperature profile for a 7-Day shipping simulation.









TABLE 3







7-Day Shipping Simulation













Duration
Total
Temp



Simulated Shipping Condition
(hrs)
time (hrs)
(° C.)







Pre-shipping hold at
40
 40
22



ambient temperature






Ambient, overnight shipping
 9
 49
22




 3
 52
35




 4
 56
22




 5
 61
35




 5
 66
22




 4
 70
35



Post-shipping hold at
 2
 72
22



ambient temperature
48
120
22




48
168
22










As an additional challenge, a shipping simulation meant to represent a “worst case” scenario was created using extreme temperatures. Table 4 show the time and temperature profile for a 3-Day extreme temperature shipping simulation.









TABLE 4







3-Day Extreme Temperature Shipping Simulation










Simulated Shipping
Duration
Total
Temp


Condition
(hrs)
time (hrs)
(° C.)













Pre-shipping hold at
16
16
22


ambient temperature





Extreme High
2.17
18.17
43.5


Temperature
6.17
24.34
33


overnight shipping
5
29.34
34



1.83
31.17
32.5



1.5
32.67
27.5



1.67
34.34
38.5



2.83
37.17
33



2.83
40
37


Post-shipping hold at
32
72
22


ambient temperature









Assay Results

The performance of the existing buffer formulation (shown below) was tested. The average percent hemoglobin recovery across all samples was determined for days 3, 5, and 7 of the 7-Day shipping simulation. These results are shown below.
















Final Concentration
Component









N/A
Water



 20 mM
1M Tris pH 7.5



   10%
BSA



 0.10%
20% Tween 20



0.095%
5% Sodium Azide



140 mM
Sodium Chloride



 10 mM
0.5M EDTA



   15 μg/mL
50 mg/mL Gentamicin










Results are listed in the table below.
















Timepoint
% Hb Recovery









Day 3
71



Day 5
68



Day 7
63










The desired % Hb Recovery at 7 days is minimally 70%. As such, the existing buffer formulation does not achieve the desired level of stability beyond 3 days (72 hours).


In an effort to improve hemoglobin stability, several classes of compounds (see Appendix A) were evaluated as additives to the base buffer formulation (also referred to herein as the existing buffer formulation) described above.


These compounds were tested at various concentrations and in combination. A list of the compounds tested using the accelerated shipping simulation and the results of these assays are found in Appendix B. A list of the compounds tested using the 3-day shipping simulation and their results are found in Appendix C. A list of the combinations of compounds that were tested is shown in Appendix D.


Of the compounds evaluated, several were identified that show potential in enhancing the stability of hemoglobin as additives to the existing buffer formulation. These compounds are listed in the table below:













Compound



Classification
Compound







Sugars/Saccharides
Trehalose



Sucrose



Polygalacturonic Acid (Pectic Acid)


Osmolytes/
Betaine


Extremolytes
Trimethylamine N-oxide


Polyvalent Ions/
Magnesium Sulfate


Metal Salts
Calcium Chloride


HRP Stabilizer
HRP Conjugate Stabilizer (PN 85R-102)


Components
HRP Conjugate Stabilizer (PN SZ02)



HRP Conjugate Stabilizer (PN ab171537)









The compounds demonstrating the most potential for enhancing Hb stability from the accelerated shipping and other simulations were tested on three stool samples in several different shipping simulations. These results are shown below.


In a 3-Day (72 hour) shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). See the following table:












3-Day Shipping Simulation











Compound

Additive
% Hb



Classification
Additive
Concentration
Recovery
Notes





Hb Collection Buffer

NA
75
Current






formulation


Polymedco (Eiken)

NA
81



Buffer






Osmolytes
Betaine
 3.5M
78
Improved Hb






Stability


Sugars/Saccharides
Sucrose
0.24M
75
Improved Hb






Stability


Sugars/Saccharides
Trehalose
 0.3M
77
Improved Hb






Stability


Polyvalent Ions/
Calcium Chloride
10 mM
77
Improved Hb


Metal Salts



Stability


Polyvalent Ions/
Magnesium Sulfate
10 mM
79
Improved Hb


Metal Salts



Stability


HRP Stabilization
HRP Conjugate Stabilizer
15%
77
Improved Hb


Components
(PN 85R-102)


Stability


HRP Stabilization
HRP Conjugate Stabilizer
15% + 10 mM
97
Improved Hb


Components +
(PN 85R-102) + CaCl2


Stability


Polyvalent Ions






HRP Stabilization
HRP Conjugate Stabilizer
15% + 10 mM
87
Improved Hb


Components +
(PN 85R-102) + MgSO4


Stability


Polyvalent Ions






HRP Stabilization
HRP Conjugate Stabilizer
15%
76
Improved Hb


Components
(PN SZ02)


Stability


HRP Stabilization
HRP Conjugate Stabilizer
15% + 10 mM
88
Improved Hb


Components +
(PN SZ02) + CaCl2


Stability


Polyvalent Ions






HRP Stabilization
HRP Conjugate Stabilizer
15% + 10 mM
92
Improved Hb


Components +
(PN SZ02) + MgSO4


Stability


Polyvalent Ions









The HRP (horse radish peroxidase) stabilizers tested and shown in the tables are:


HRP Conjugate Stabilizer (PN 85R-102), Fitzgerald Industries International, 30 Sudbury Road, Suite 1A North, Acton, Mass., 01720 USA


HRP Conjugate Stabilizer (PN SZ02), Surmodics, Inc., 9924 West 74th Street, Eden Prairie, Minn. 55344 USA


HRP Conjugate Stabilizer (PN ab171537), Abcam, 1 Kendall Square, Cambridge, Mass. 02139 USA


In a 7-Day shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). See the following Table:












7-Day Shipping Simulation
















% Hb
% Hb
% Hb



Compound

Additive
Recovery
Recovery
Recovery


Classification
Additive
Concentration
Day 3
Day 5
Day 7
Notes
















Hb Collection

NA
71
68
63
Current


Buffer





formulation


Polymedco Buffer

NA
83
83
80


Polyvalent
Calcium Chloride
10 mM
77
75
73
Improved Hb


Ions/Metal Salts





Stability


Polyvalent
Magnesium
10 mM
76
74
71
Improved Hb


Ions/Metal Salts
Sulfate




Stability


Osmolytes
Betaine
3.5M
78
74
70
Improved Hb








Stability


HRP Stabilization
HRP Conjugate
15%
80
75
69
Improved Hb


Components
Stabilizer (PN 85R-




Stability



102)


HRP Stabilization
HRP Conjugate
15% + 10 mM
89
88
82
Improved Hb


Components +
Stabilizer (PN 85R-




Stability


Polyvalent Ions
102) + CaCl2


HRP Stabilization
HRP Conjugate
15% + 10 mM
89
88
82
Improved Hb


Components +
Stabilizer (PN 85R-




Stability


Polyvalent Ions
102) + MgSO4


HRP Stabilization
HRP Conjugate
15%
80
70
61
Improved Hb


Components
Stabilizer (PN




Stability



SZ02)


HRP Stabilization
HRP Conjugate
15% + 10 mM
86
83
78
Improved Hb


Components +
Stabilizer (PN




Stability


Polyvalent Ions
SZ02) + CaCl2


HRP Stabilization
HRP Conjugate
15% + 10 mM
90
85
79
Improved Hb


Components +
Stabilizer (PN




Stability


Polyvalent Ions
SZ02) + MgSO4









In a 3-Day extreme temperature shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). Additives containing HRP stabilization buffer and Calcium Chloride or Magnesium Sulfate also demonstrate improved Hb stability compared to the Polymedco buffer. See following Table:












3-Day Extreme Temperature Shipping Simulation











Compound

Additive
% Hb



Classification
Additive
Concentration
Recovery
Notes





Hb Collection
NA
NA
47
Current


Buffer



formulation


Polymedco Buffer
NA
NA
67



Polyvalent Ions/
Calcium Chloride
10 mM
58
Improved Hb


Metal Salts



Stability


Polyvalent Ions/
Magnesium Sulfate
10 mM
54
Improved Hb


Metal Salts



Stability


Osmolytes
Betaine
3.5M
61
Improved Hb






Stability


HRP Stabilization
HRP Conjugate Stabilizer
15%
56
Improved Hb


Components
(PN 85R-102)


Stability


HRP Stabilization
HRP Conjugate Stabilizer
15% and 10 mM
77
Improved Hb


Components +
(PN 85R-102) + CaCl2


Stability


Polyvalent Ions






HRP Stabilization
HRP Conjugate Stabilizer
15% and 10 mM
71
Improved Hb


Components +
(PN 85R-102) + MgSO4


Stability


Polyvalent Ions









In an accelerated shipping simulation, the additive polygalacturonic acid (with calcium chloride) is demonstrating improved Hb stability over the existing formulation (Hb Collection Buffer) at different concentrations. See the following table:












Accelerated Shipping Simulation











Compound

Additive
% Hb



Classification
Additive
Concentration
Recovery
Notes





Hb Collection
NA
NA
72
Current


Buffer



formulation


Sugars/
Polygalacturonic Acid +
 0.01% + 10 mM
83
Improved Hb


Saccharides
Calcium Chloride


Stability



Polygalacturonic Acid +
0.025% + 10 mM
91
Improved Hb



Calcium Chloride


Stability



Polygalacturonic Acid +
 0.05% + 10 mM
86
Improved Hb



Calcium Chloride


Stability



Polygalacturonic Acid +
0.075% + 10 mM
79
Improved Hb



Calcium Chloride


Stability



Polygalacturonic Acid +
  0.1% + 10 mM
64
No Improvement



Calcium Chloride






Polygalacturonic Acid +
0.125% + 10 mM
86
Improved Hb



Calcium Chloride


Stability









Another class of compounds called protoporphyrins was tested and can enhance the stability of hemoglobin when added to the existing buffer formulation. The protoporphyrins tested are shown in the table below. Note that in some test cases the pH was altered as solubility of protoporphyrins is known to be sensitive to pH.









TABLE 14







Additional Potential Compounds










Compound Classification
Compound






Protoporphyrins
Protoporphyrin IX Zinc




Protoporphyrin IX Cobalt




Protoporphyrin IX Chromium




Hemin (Protoporphyrin IX Iron)




Hematin (Protoporphyrin IX Iron)









In a 3-Day (72 hour) shipping simulation the existing formulation with added protoporphyrin provided improved stability over the existing formulation without any protoporphyrin). These results are shown in the table below.












3-Day Shipping Simulation











Compound

Additive




Classification
Additive
Concentration
% Hb Recovery
Notes





Hb Collection

NA
70
Current


Buffer



formulation


Polymedco

NA
80



(Eiken) Buffer






Protoporphyrin
Hemin (Buffer pH 6.5)
0.0003%
88
Improved




(4.8 μM)

Hb Stability


Protoporphyrin
Hemin (Buffer pH 7.4)
0.0003%
78
Improved




(4.8 μM)

Hb Stability


Protoporphyrin
Protoporphyrin IX Zn
1 μM
89
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Zn
1 μM
75
Improved



(Buffer pH 7.4)


Hb Stability


Protoporphyrin
Protoporphyrin IX Cr
2.5 μM  
117 
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Cr
2.5 μM  
102 
Improved



(Buffer pH 7.4)


Hb Stability


Protoporphyrin
Protoporphyrin IX Co
5 μM
109 
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Co
5 μM
111 
Improved



(Buffer pH 7.4)


Hb Stability









In a 7-day shipping simulation the existing formulation with added protoporphyrin provided improved stability over the existing formulation without any protoporphyrin. These results are shown in the table below.












7-Day Shipping Simulation
















% Hb
% Hb
% Hb



Compound

Additive
Recovery
Recovery
Recovery


Classification
Additive
Concentration
Day 3
Day 5
Day 7
Notes
















Hb Collection Buffer

NA
70
64
60
Current








formulation


Polymedco (Eiken)

NA
83
82
80


Buffer


Protoporphyrin
Hemin (Buffer
0.0003%
85
81
80
Improved



pH 6.5)
(4.8 μM)



Hb Stability


Protoporphyrin
Hemin (Buffer
0.0003%
78
77
68
Improved



pH 7.4)
(4.8 μM)



Hb Stability














Protoporphyrin
Protoporphyrin
1
μM
84
83
80
Improved



IX Zn (Buffer





Hb Stability



pH 6.5)


Protoporphyrin
Protoporphyrin
1
μM
73
70
65
Improved



IX Zn





Hb Stability



(Buffer pH 7.4)


Protoporphyrin
Protoporphyrin
2.5
μM
109
113
110
Improved



IX Cr





Hb Stability



(Buffer pH 6.5)


Protoporphyrin
Protoporphyrin
2.5
μM
93
91
90
Improved



IX Cr





Hb Stability



(Buffer pH 7.4)


Protoporphyrin
Protoporphyrin
5
μM
101
104
104
Improved



IX Co





Hb Stability



(Buffer pH 6.5)


Protoporphyrin
Protoporphyrin
5
μM
103
99
93
Improved



IX Co





Hb Stability



(Buffer pH 7.4)









Adding protoporphyrins to the existing formulation (Hb Collection Buffer) also improves Hb stability in 3-Day extreme temperature shipping simulation. In addition, additives containing protoporphyrin cobalt (Co) or protoporphyrin chromium (Cr) demonstrate improved Hb stability compared to the Polymedco buffer. See the table below:












3-Day Extreme Temperature Shipping Simulation











Compound

Additive




Classification
Additive
Concentration
% Hb Recovery
Notes





Hb Collection
NA
NA
46
Current


Buffer



formulation


Polymedco Buffer
NA
NA
69



Protoporphyrin
Hemin (Buffer pH 6.5)
0.0003%
67
Improved




(4.8 μM)

Hb Stability


Protoporphyrin
Hemin (Buffer pH 7.4)
0.0003%
54
Improved




(4.8 μM)

Hb Stability


Protoporphyrin
Protoporphyrin IX Zn
1 μM
64
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Cr
2.5 μM  
92
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Cr
2.5 μM  
68
Improved



(Buffer pH 7.4)


Hb Stability


Protoporphyrin
Protoporphyrin IX Co
5 μM
81
Improved



(Buffer pH 6.5)


Hb Stability


Protoporphyrin
Protoporphyrin IX Co
5 μM
73
Improved



(Buffer pH 7.4)


Hb Stability









Further Testing of Protoporphyrin

Protoporphyrin IX was tested at various concentrations and pHs using the 7-Day Modified Shipping Simulation conditions listed below as well as other conditions.












7-Day Modified Shipping Simulation












Simulated Shipping
Duration
Total Time
Temp



Condition
(hrs)
(hrs)
(° C.)






Pre-shipping hold at ambient
72 
 72
22



temperature






Pre-shipping temperature spike
8
 80
35



Pre-shipping hold at ambient
16 
 96
22



temperature






Ambient, overnight shipping
24 
120
22




9
129
22




3
132
35




4
136
22




5
141
35




5
146
22




4
150
35



Post-shipping hold at ambient
18 
168
22



temperature









The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 3-6 μM at an adjusted pH range of 6.4-7.0 provide increased Hb stability in the 7-Day Modified Shipping Simulation.












7-Day Modified Shipping Simulation












Compound







Classification
Additive
Additive Conc.
Buffer pH
% Hb Recovery
Notes















Hb Collection
NA
NA
7.4
59.4
Current


Buffer




Formulation


Polymedco
NA
NA
NA
70



Buffer (Eiken)







Protoporphyrins
Protoporphyrin
3 μM
6.9
75.0
Improved Hb



IX Co



Stability



Protoporphyrin
3.5 μM  
6.9
82.2
Improved Hb



IX Co



Stability



Protoporphyrin
4 μM
6.4
100.0
Improved Hb



IX Co



Stability



Protoporphyrin
4 μM
6.6
94.8
Improved Hb



IX Co



Stability



Protoporphyrin
4 μM
6.8
93.3
Improved Hb



IX Co



Stability



Protoporphyrin
4 μM
6.9
84.8
Improved Hb



IX Co



Stability



Protoporphyrin
4 μM
7.0
87.7
Improved Hb



IX Co



Stability



Protoporphyrin
4.5 μM  
6.9
84.6
Improved Hb



IX Co



Stability



Protoporphyrin
5 μM
6.4
105.0
Improved Hb



IX Co



Stability



Protoporphyrin
5 μM
6.6
102.0
Improved Hb



IX Co



Stability



Protoporphyrin
5 μM
6.8
95.5
Improved Hb



IX Co



Stability



Protoporphyrin
5 μM
6.9
84.1
Improved Hb



IX Co



Stability



Protoporphyrin
5 μM
7.0
95.7
Improved Hb



IX Co



Stability



Protoporphyrin
6 μM
6.4
110.0
Improved Hb



IX Co



Stability



Protoporphyrin
6 μM
6.6
106.0
Improved Hb



IX Co



Stability



Protoporphyrin
6 μM
6.8
101.0
Improved Hb



IX Co



Stability



Protoporphyrin
6 μM
7.0
95.0
Improved Hb



IX Co



Stability









The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 2.5-10 μM at an adjusted pH of 6.8 provide increased Hb stability in the 3 and 7 day Shipping Simulations.


The following table shows that buffers containing Chromium(III) PPIX (Protoporphyrin IX) at concentrations of 0.5-10 μM at an adjusted pH of 7.1 provide increased Hb stability in the 3 and 7 day Shipping Simulations.












3-Day and 7-Day Shipping Simulation

















3-Day
7-Day



Compound

Additive
Buffer
% Hb
% Hb


Class
Additive
Conc.
pH
Recovery
Recovery
Notes
















Hb Collection
NA
NA
7.4
61.5
54.8
Current


Buffer





Formulation














Protoporphyrin
Protoporphyrin
2.5
μM
6.8
80.2
79.5
Improved Hb



IX Co





Stability




3
μM
6.8
76.0
73.4
Improved Hb









Stability




5
μM
6.8
82.8
82.7
Improved Hb









Stability




7
μM
6.8
86.5
83.7
Improved Hb









Stability




7.5
μM
6.8
92.9
94.8
Improved Hb









Stability




10
μM
6.8
95.5
101.3
Improved Hb









Stability



Protoporphyrin
0.5
μM
7.1
75.0
65.6
Improved Hb



IX Cr





Stability




2.5
μM
7.1
86.3
78.9
Improved Hb









Stability




4.5
μM
7.1
94.5
86.4
Improved Hb









Stability




5
μM
7.1
97.0
99.4
Improved Hb









Stability




7.5
μM
7.1
108.7
112.7
Improved Hb









Stability




10
μM
7.1
116.8
113.8
Improved Hb









Stability









The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 2.5-10 μM at an adjusted pH range of 6.2-7.4 provide increased Hb stability in the 35 C 16 hour challenge.


The following table shows that buffers containing Chromium(III) PPIX (Protoporphyrin IX) at concentrations of 1.25-5 μM at an adjusted pH range of 6.2-7.4 provide increased Hb stability in the 35 C 16 hour challenge.












35C 16 hour challenge












Compound







Class.
Additive
Additive Conc.
Buffer PH
% Recovery
Notes















Hb Collection
NA
NA
7.4
71.2
Current


Buffer




Formulation


Protoporphyrin
Protoporphyrin
2.5 μM
6.2
76.0
Improved Hb



IX Co



Stability




2.5 μM
6.5
84.1
Improved Hb







Stability




2.5 μM
6.8
82.3
Improved Hb







Stability




2.5 μM
7.1
80.4
Improved Hb







Stability




5.0 μM
6.2
104.3
Improved Hb







Stability




5.0 μM
6.5
105.5
Improved Hb







Stability




5.0 μM
6.8
93.8
Improved Hb







Stability




5.0 μM
7.1
85.1
Improved Hb







Stability




5.0 μM
7.4
78.4
Improved Hb







Stability




 10 μM
6.2
116.0
Improved Hb







Stability




 10 μM
6.8
107.6
Improved Hb







Stability




 10 μM
7.1
112.9
Improved Hb







Stability




 10 μM
7.4
98.5
Improved Hb







Stability


Protoporphyrin
Protoporphyrin
1.25 μM 
6.2
74.0
Improved Hb



IX Cr



Stability




1.25 μM 
6.5
77.0
Improved Hb







Stability




1.25 μM 
6.8
105.0
Improved Hb







Stability




1.25 μM 
7.1
77.5
Improved Hb







Stability




1.25 μM 
7.4
73.5
Improved Hb







Stability




2.5 μM
6.2
79.8
Improved Hb







Stability




2.5 μM
6.5
86.3
Improved Hb







Stability




2.5 μM
6.8
85.0
Improved Hb







Stability




2.5 μM
7.1
81.8
Improved Hb







Stability




2.5 μM
7.4
73.1
Improved Hb







Stability




  5 μM
6.2
87.5
Improved Hb







Stability




  5 μM
6.5
89.6
Improved Hb







Stability




  5 μM
7.1
86.0
Improved Hb







Stability




  5 μM
7.4
75.5
Improved Hb







Stability









Accordingly, the preceding merely illustrates the principles of the invention. It will be appreciated that those skilled in the art will be able to devise various arrangements which, although not explicitly described or shown herein, embody the principles of the invention and are included within its spirit and scope. Furthermore, all examples and conditional language recited herein are principally intended to aid the reader in understanding the principles of the invention and the concepts contributed by the inventors to furthering the art and are to be construed as being without limitation to such specifically recited examples and conditions. Moreover, all statements herein reciting principles, aspects, and embodiments of the invention as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents and equivalents developed in the future, i.e., any elements developed that perform the same function, regardless of structure. The scope of the present invention, therefore, is not intended to be limited to the exemplary embodiments shown and described herein. Rather, the scope and spirit of the present invention is embodied by the appended claims.












APPENDIX A








Compound



Classification
Compound





Metal Chelators
Nitrilotriacetic acid (NTA)



Ethylenediamine-N,N′-disuccinic acid (EDDS)



diethylenetriaminepentaacetic acid (DTPA)



Iminodisuccinic acid (IDS; ISA)



N,N-bis-(Carboxymethyl)-L-glutamic acid tetrasodiumn salt (GLDA)



Trisodium N-(1-Carboxylatoethyl)iminodiacetate Hydrate (MGDA)



Phytic Acid (IP6)



ATMP - Amino tris (methylene phosphonic acid)



Iminodiacetic Acid (IDA)



sodium tripolyphosphate



ethylenediamine-N,N,N′,N′-4-acetic acid (EDTA)


Flavoniods
quercetin



chrysin-pyridine



daidzein - DMSO



7,8-Dihydroxyflavone


Protease/
aurintircarboxylic acid ammonium salt


Phosphatase Inhibitors
methylisothiazolone



Proclin 300



sodium fluoride


Crosslinkers
poly(acrylic acid)



4arm-PEG20K-Maleimide



mPEG-Maleimide, 5K



glutaraldehyde



Dimethyl 3,3′-dithiopropionimidate dihydrochloride



bis(3,5-dibromosalicyl) fumarate



formaldehyde solution



magnesium gluconate



cysteamine



methylglyoxal


Similar to
Imidazole


Drabkin's Reagent
hydroxylamine


(cyanide)
Histidine


Non-covalent
polysufonated polymers (dextran sulphate)


Hb interactions
heparin



PEG 2000



PEG 20000



PEG 8000



Bovine Serum Albumin



Poly viny alcohol


Sulfonic Acids
methanedisulfonic acid dipotassiurn salt



propanedisulfonic acid disodium sat



piperazine-N-N′-bis (2-ethanesulfonic acid) (PIPES)


Preservatives with
Sodium Thiosulfate


thiol groups
Cysteine



glutathione



dithiothreitol



TCEP


Sugars/Saccharides
trehalose



Glucose



raffinose



corn starch



potato starch



Gum arabic



xanthan gum



Lactose



Maltose



Fructose



Galactose



Isomaltotriose



Maltotriose



Melezitose



Kestose



Stachyose



Glucose Tetrasaccharide



Erlose



Mannose



Polygalacturonic Acid



Galacturonic Acid (monomer)



Sodium Alginate (synthetic)



Sodium Alginate (Medium Viscosity)



Sodium Alginate (Low Viscosity)


Redox/antioxidants
lipoic acid



mPEG2K-Thioctic acid



ascorbic acid



glutathione



Deferoxamine mesylate salt



uric acid



urea


Formaldehyde Releasers
Imidazolidinyl Urea



Bronopol



Diazolidinyl Urea



DMDM Hydantoin



Sodium hydroxymethyl glycinate



Tris(Hydroxymethyl)nitromethane



5-bromo-5-nitro-1,3-dioxane



2-bromo-2-nitro-1,3-propanediol



1,3-Bis(hydroxymethyl)-5,5-dimethylimidazolidine-2,4-dione



Streck Cell Preservative Solution



1-(cis-3-Chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride


Substrates
Styrene Glycol



Styrene Oxide



Sodium Bicarbonate



DPG, diphospho glycerate



Arginine



Lysine



Resveratrol


Protoporphyrins
Protoporphyrin IX Zn



Protoporphyrin IX



Protoporphyrin IX Cobalt



Protoporphyrin IX Chromium



Protoporphyrin IX Magnesium



Protoporphyrin IX Manganese



Protoporphyrin IX Tin



Protoporphyrin IX Gallium



Hemin, Bovine



Hematin



Bilirubin



Biliverdin


Osmolytes/Extremolytes
Betaine



Trimethylamine N-oxide



Ectoine



Firoin


Formaldehyde +
paraformaldehyde


Ammonium Salt
formaldehyde solution


Complexes
ammonium chloride



ammonium sulfate



ammonium acetate



ammonium oxalate



hexamethylenetetramine


Ionic Liquids
1-Allyl-3-methylimidazolium chloride



1-Butyl-3-methylimidazolium chloride



1-butyl-2,3-dimethylimidazolium tetrafluoroborate



1-butyl-3-methylimidazoliumtetrafluoroborate



1-Ethyl-3-methylimidazolium chloride



2-(Diethylamino)ethanol



Methyl hexanoate



1-hexyl-3-methylimidazolium chloride



1-decyl-3-methylimidazolium chloride



Sodium Bromide


Deep Eutectic Solvents
Choline Chloride



Tetrabutylammonium bromide



urea



Imidazole



Glycerol



Ethylene Glycol



D-glucose


Polyvalent Ions/
Fe (III) Chloride


Metal Salts
Cobalt



Nickel



Copper



Zinc



Sodium Bisulfite



Sodium Carbonate



Sodium Perchlorate



Sodium Phosphate



Sodium Acetate



Potassium Chloride



Magnesium Chloride



Potassium Acetate



Lithium Chloride



Iron (II) Sulfate



Magnesium Sulfate



Zinc chloride



Calcium Chloride



Calcium Gluconate



Calcium Propoinate


HRP Stabilizer
Calcium Propoinate


Components
8-anilino-1-napthalene sulfonic acid ammonium salt



aluminum chloride hexahydrate



4-bromophenol



2-methyl-4-isothiazolin-3-one-hydrochloride



5-bromo-5-nitro-1,3-dioxane



AAT Bioquest HRP Stabilization Buffer



Azobenzene



Phenol Red



Proclin 300



Candor HRP Stabilization Buffer



Innova HRP Stabilization Buffer



Surmodics HRP Stabilization Buffer



BioRad HRP Stabilization Buffer



Sigma HRP Stabilization Buffer



Guardian Peroxidase Conjugate Buffer



Fitzgerald HRP Conjugate Buffer



Sigma HRP Stabilization Buffer


Vitamins
Pyridoxal 5′-phosphate hydrate (B6)



NAD



Vitamin B12



NADH



Nicotinic Acid (B3)



Folic Acid (M)



Nicotinamide (B3)



Vitamin E (alpha tocopherol)


Preservatives
Sodium Nitrate



Sodium Nitrite



Sodium Citrate



Sodium Benzoate


Preservatives/Parabens
Ethyl 4-hydroxybenzoate



Methyl 4-hydroxybenzoate



Propyl 4-hydroxybenzoate



Benzyl 4-hydroxybenzoate


Aldehydes
formaldehyde solution



paraformaldehyde



Glutaraldehyde



Furfural



p-Tolualdehyde



Cinnamaldehyde



Butyraldehyde



Glycolaldehyde Dimer



Glyceraldehyde



Formamide



4-Isobutylbenzaldehyde



Benzaldehyde



Acetylaldehyde



Vanillin


Plasma
Human Plasma



Albumin



Glycine



HDL



LDL



Phosphatidylcholine



Cohns Fraction II/III



Cohns Fraction IV



Fetal Bovine Serum



Bovine Plasma



Gamma Globulins


Gelatins
Gelatin-Bovine Type B (225 g bloom)



Gelatin-Porcine Type A (90-110 g bloom)



Gelatin-Porcine Type A (175 g bloom)



Gelatin-Porcine Type A (300 g bloom)



Gelatin-Cold Water Fish Skin


Detergents
Brij 35



Tween 20



Triton X-100


Solvents
1N HCl



1M NaOH



1M Tris, pH 7.5



pyridine



DMSO



















APPENDIX B














% Hb



Compound aassification
Additive
Additive Concentration
Recovery
Results














None
Hb Collection Buffer - No additive
NA
68
Current Buffer






Formulation


None
Polymedco Buffer - No additive
NA
80



Ionic Liquids
1-ethyl-3-methylimidazolium chloride
1 mM
68
No Improvement


Ionic Liquids
1-ethyl-3-methylimidazolium chloride
10 mM
69
No Improvement


Ionic Liquids
1-ethyl-3-methylimidazolium chloride
100 mM
65
No Improvement


Ionic Liquids
1-hexyl-3-methylimidazolium chloride
0.02%
67
No Improvement


Ionic Liquids
1-hexyl-3-methylimidazolium chloride
0.20%
61
No Improvement


Ionic Liquids
1-hexyl-3-methylimidazolium chloride
1%
42
No Improvement


Protoporphyrin
Protoprophyrin IX
0.001 mM
65
No Improvement


Protoporphyrin
Protoprophyrin IX
0.01 mM
62
No Improvement


Protoporphyrin
Protoprophyrin IX
0.1 mM
63
No Improvement


Protoporphyrin
Protoprophyrin IX Zn
0.001 mM
68
No Improvement


Protoporphyrin
Protoprophyrin IX Zn
0.01 mM
59
No Improvement


Protoporphyrin
Protoprophyrin IX Zn
0.1 mM
53
No Improvement


Formaldehyde Releaser
Sodium hydroxylmethyl glycinate
0.1 mM
70
No Improvement


Formaldehyde Releaser
Sodium hydroxylmethyl glycinate
1 mM
64
No Improvement


Formaldehyde Releaser
Sodium hydroxylmethyl glycinate
10 mM
66
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
50 mM
76
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
100 mM
72
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
200 mM
74
No Improvement


Protoporphyrin
Protoprophyrin IX + FeCl3
0.1 mM + 0.1 mM
62
No Improvement


Protoporphyrin
Protoprophyrin IX + FeCl3
1 mM + 1 mM
53
No Improvement


Protoporphyrin
Protoprophyrin IX + FeCl3
10 mM + 10 mM
40
No Improvement


Crosslinkers
Poly Acrylic Acid
1 mM
64
No Improvement


Crosslinkers
Poly Acrylic Acid
5 mM
56
No Improvement


Crosslinkers
Poly Acrylic Acid
7 mM
58
No Improvement


Crosslinkers
4arm-PEG2K-Maleimide + Iminothiolane
0.062 mM + 1 mM
67
No Improvement



hydrochloride





Crosslinkers
4arm-PEG2K-Maleimide + Iminothiolane
0.125 mM + 2 mM
66
No Improvement



hydrochloride





Crosslinkers
4arm-PEG2K-Maleimide + Iminothiolane
2.5 mM + 10 mM
27
No Improvement



hydrochloride





Crosslinkers
mPEG-Mal, 5K + Iminothiolane
0.5 mM + 1 mM
65
No Improvement



hydrochloride





Crosslinkers
mPEG-Mal, 5K + Iminothiolane
1 mM + 2 mM
58
No Improvement



hydrochloride





Crosslinkers
mPEG-Mal, 5K + Iminothiolane
5 mM + 10 mM
56
No Improvement



hydrochloride





Plasma
Human Plasma, lyopholyzed
1%
97
High Background Signal


Plasma
Human Plasma, lyopholyzed
5%
98
High Background Signal


Plasma
Human Plasma, lyopholyzed
10%
97
High Background Signal


Plasma
Human Plasma, lyopholyzed
25%
94
High Background Signal


Plasma
Human Plasma, lyopholyzed
50%
95
High Background Signal


Plasma
Human Plasma, lyopholyzed
1%
96
High Background Signal


Plasma
Human Plasma, lyopholyzed
5%
97
High Background Signal


Plasma
Human Plasma, lyopholyzed
10%
96
High Background Signal


Plasma
Human Plasma, lyopholyzed
25%
98
High Background Signal


Plasma
Human Plasma, lyopholyzed
50%
99
High Background Signal


Plasma
Human Plasma, lyopholyzed
1%
97
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.50%
92
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.10%
99
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.05%
101 
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.01%
81
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.005%
81
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.001%
71
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.005%
77
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.01%
80
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.02%
88
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.03%
93
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.04%
97
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.05%
95
High Background Signal


Plasma
Human Plasma, lyopholyzed
0.10%
99
High Background Signal


Plasma
Albumin, Human
0.0025 mg/mL
71
No Improvement


Plasma
Albumin, Human
0.025 mg/mL
90
No Improvement


Plasma
Albumin, Human
0.25 mg/mL
98
No Improvement


Plasma
Albumin, Human
2.5 mg/mL
99
No Improvement


Plasma
Albumin, Human
25 mg/mL
98
No Improvement


Plasma
Glycine
0.0002 mg/mL
68
No Improvement


Plasma
Glycine
0.002 mg/mL
68
No Improvement


Plasma
Glycine
0.02 mg/mL
71
No Improvement


Plasma
Cohn's Fraction II/III
0.005 mg/mL
70
High Background Signal


Plasma
Cohn's Fraction II/III
0.05 mg/mL
80
High Background Signal


Plasma
Cohn's Fraction II/III
0.5 mg/mL
99
High Background Signal


Plasma
Cohn's Fraction II/III
5 mg/mL
100 
High Background Signal


Plasma
Cohn's Fraction II/III
50 mg/mL
97
High Background Signal


Plasma
Cohn's Fraction IV
0.005 mg/mL
106 
High Background Signal


Plasma
Cohn's Fraction IV
0.05 mg/mL
100 
High Background Signal


Plasma
Cohn's Fraction IV
0.5 mg/mL
98
High Background Signal


Plasma
Cohn's Fraction IV
5 mg/mL
96
High Background Signal


Plasma
Cohn's Fraction IV
50 mg/mL
98
High Background Signal


Plasma
HDL
0.0025 mg/mL
69
No Improvement


Plasma
HDL
0.025 mg/mL
69
No Improvement


Plasma
HDL
0.25 mg/mL
70
No Improvement


Plasma
LDL
0.0025 mg/mL
69
No Improvement


Plasma
LDL
0.025 mg/mL
70
No Improvement


Plasma
LDL
0.25 mg/mL
67
No Improvement


Plasma
phosphatidylcholine
0.01 mg/mL
67
No Improvement


Plasma
phosphatidylcholine
0.1 mg/mL
66
No Improvement


Plasma
phosphatidylcholine
1 mg/mL
62
No Improvement


Polyvalent Ions/Metal Salts
Calcium Chloride
1 mM
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
78
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
100 mM
119 
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Deep Eutectic Solvents
Ethylene Glycol
5%
69
No Improvement


Deep Eutectic Solvents
Ethylene Glycol
10%
63
No Improvement


Deep Eutectic Solvents
Ethylene Glycol
20%
54
No Improvement


Polyvalent Ions/Metal Salts
Ethylene Glycol + Calcium Chloride
10% + 10 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Ethylene Glycol + Calcium Chloride
2.5% + 2.5 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Ethylene Glycol + Calcium Chloride
5% + 5 mM
72
No Improvement


Non-covalent Hb interactions
Dextran Sulphate
3.75%
67
No Improvement


Non-covalent Hb interactions
Dextran Sulphate
7.50%
63
No Improvement


Non-covalent Hb interactions
Dextran Sulphate
15%
70
No Improvement


Osmolytes
Betaine
125 mM
72
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
250 mM
76
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
500 mM
78
Betaine shows improvement






in % Hb Recovery at high






concentrations


Sugars/Saccharides
Trehalose + Betaine
125 mM + 62.5 mM
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Betaine
250 mM + 125 mM
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Betaine
500 mM + 250 mM
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
250 mM + 62.5 mM
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
500 mM + 125 mM
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
1M + 250 mM
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycerol
250 mM + 2.5%
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycerol
500 mM + 5%
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycerol
1M + 10%
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycine
250 mM + 62.5 mM
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycine
500 mM + 125 mM
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Glycine
1M + 250 mM
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Glycerol
125 mM + 2.5%
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Glycerol
250 mM + 5%
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Glycine
125 mM + 62.5 mM
70
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Glycine
250 mM + 125 mM
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Glycine
500 mM + 250 mM
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
1M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Polyvalent Ions/Metal Salts
Calcium Chloride
5 mM
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
67
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
25 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
50 mM
68
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
100 mM
51
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 2000
0.075% + 1%
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 2000
0.15% + 2%
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 2000
0.3% + 4%
62
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 20000
0.075% + 1%
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 20000
0.15% + 2%
72
Calcium cation shows






improvementin % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 20000
0.3% + 4%
66
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 8000
0.075% + 1%
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 8000
0.15% + 2%
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + PEG 8000
0.3% + 4%
67
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Formaldehyde + Ammonium
Ammonium Acetate
100 mM
58
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Acetate
25 mM
58
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Acetate
50 mM
59
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Chloride
100 mM
58
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Chloride
25 mM
104 
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Chloride
50 mM
58
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Oxalate
10 mM
62
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Oxalate
2.5 mM
60
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Oxalate
5 mM
59
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Sulfate
100 mM
61
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Sulfate
25 mM
60
No Improvement


Salt Complexes






Formaldehyde + Ammonium
Ammonium Sulfate
50 mM
58
No Improvement


Salt Complexes






Polyvalent Ions/Metal Salts
Calcium Gluconate
100 mM
57
No Improvement


Polyvalent Ions/Metal Salts
Calcium Gluconate
25 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Calcium Gluconate
50 mM
65
No Improvement


Sugars/Saccharides
Glucose + Calcium Chloride
0.5M + 0.5%
65
No Improvement


Sugars/Saccharides
Glucose + Calcium Chloride
1M + 1%
65
No Improvement


Sugars/Saccharides
Glucose + Calcium Chloride
2M + 2%
61
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Gluconate
100 mM
58
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Gluconate
25 mM
69
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Gluconate
50 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
0.1 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
1 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
2 mM
72
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
4 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
6 mM
77
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
8 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
10 mM
79
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (anhydrous)
20 mM
76
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
0.1 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
1 mM
70
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
2 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
4 mM
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
6 mM
74
Calcium cation shows






improvemen in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
8 mM
72
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
10 mM
77
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride (dihydrate)
20 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Formaldehyde Releaser
5-bromo-5-nitro-1,3-dioxane
0.0002%
69
No Improvement


Formaldehyde Releaser
5-bromo-5-nitro-1,3-dioxane
0.001%
68
No Improvement


Formaldehyde Releaser
5-bromo-5-nitro-1,3-dioxane
0.002%
70
No Improvement


Enzyme Inhibitor
2-methy1-4-isothiazolin-3-one-hydrochloride
0.0002%
71
No Improvement


Enzyme Inhibitor
2-methy1-4-isothiazolin-3-one-hydrochloride
0.001%
68
No Improvement


Enzyme Inhibitor
2-methy1-4-isothiazolin-3-one-hydrochloride
0.002%
63
No Improvement


HRP Stabilizer Components
Phenol Red
0.01%
70
No Improvement


HRP Stabilizer Components
Phenol Red
0.03%
71
No Improvement


HRP Stabilizer Components
Phenol Red
0.05%
70
No Improvement


Preservatives
Sodium Benzoate
0.10%
67
No Improvement


Preservatives
Sodium Benzoate
0.50%
60
No Improvement


Preservatives
Sodium Benzoate
1%
54
No Improvement


HRP Stabilizer Components
Sigma HRP Stabilization Buffer
5%
71
No Improvement


HRP Stabilizer Components
Sigma HRP Stabilization Buffer
10%
70
No Improvement


HRP Stabilizer Components
Sigma HRP Stabilization Buffer
25%
68
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
5% Additive
83
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
5% Component
71
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Gluconate
100 mM
57
No Improvement


Polyvalent Ions/Metal Salts
Calcium Gluconate
25 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Calcium Gluconate
50 mM
65
No Improvement


Sugars/Saccharides
Glucose + Calcium Chloride
0.5M + 0.5%
65
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Calcium Chloride
1M + 1%
65
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Calcium Chloride
2M + 2%
61
Slight Improvement with






Sugars. Needs further assay






optimization


Polyvalent Ions/Metal Salts
Magnesium Gluconate
100 mM
58
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Gluconate
25 mM
69
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Gluconate
50 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Iron (2) Chloride
36 mM
60
No Improvement


Polyvalent Ions/Metal Salts
Iron (2) Chloride
43.2 mM
51
No Improvement


Polyvalent Ions/Metal Salts
Iron (2) Chloride
48 mM
27
No Improvement


Polyvalent Ions/Metal Salts
Lithium Chloride
100 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Lithium Chloride
150 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Lithium Chloride
260 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Chloride
100 mM
62
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
150 mM
61
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
192 mM
57
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Potassium Acetate
100 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Potassium Acetate
150 mM
69
No Improvement


Polyvalent Ions/Metal Salts
Potassium Acetate
260 mM
67
No Improvement


Polyvalent Ions/Metal Salts
Potassium Chloride
100 mM
53
No Improvement


Polyvalent Ions/Metal Salts
Potassium Chloride
150 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Potassium Chloride
260 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Sodium Acetate
100 mM
55
No Improvement


Polyvalent Ions/Metal Salts
Sodium Acetate
150 mM
40
No Improvement


Polyvalent Ions/Metal Salts
Sodium Acetate
260 mM
45
No Improvement


Substrates
Sodium Bicarbonate
100 mM
46
No Improvement


Substrates
Sodium Bicarbonate
150 mM
43
No Improvement


Substrates
Sodium Bicarbonate
260 mM
42
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bisulfite
101.055 mM
38
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bisulfite
75.791 mM
46
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bisulfite
90.95 mM
41
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bromide
100 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bromide
150 mM
64
No Improvement


Polyvalent Ions/Metal Salts
Sodium Bromide
192 mM
59
No Improvement


Polyvalent Ions/Metal Salts
Sodium Carbonate
38.207 mM
28
No Improvement


Polyvalent Ions/Metal Salts
Sodium Carbonate
45.848 mM
24
No Improvement


Polyvalent Ions/Metal Salts
Sodium Carbonate
50.942 mM
25
No Improvement


Polyvalent Ions/Metal Salts
Sodium Perchlorate
100 mM
47
No Improvement


Polyvalent Ions/Metal Salts
Sodium Perchlorate
150 mM
43
No Improvement


Polyvalent Ions/Metal Salts
Sodium Perchlorate
260 mM
38
No Improvement


Polyvalent Ions/Metal Salts
Sodium Phosphate
100 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Sodium Phosphate
150 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Sodium Phosphate
260 mM
66
No Improvement


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
10%
78
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
11%
77
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
12%
78
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
13%
81
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
14%
80
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
15%
81
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
16%
90
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
17%
81
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
18%
87
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
19%
85
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
20%
83
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
21%
86
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
22%
86
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
23%
89
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
24%
86
Slight Improvement. Needs






further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
25%
84
Slight Improvement. Needs






further assay optimization


Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
1% (50 g/mL, 100 mL/L,
34
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
5% (50 g/mL, 100 mL/L,
62
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
10% (50 g/mL, 100 mL/L,
58
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
25% (50 g/mL, 100 mL/L,
62
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
50% (50 g/mL, 100 mL/L,
30
No Improvement




5.8 g/L)




Plasma
Bovine B
1 g/L
68
No Improvement


Plasma
Bovine B
2 g/L
65
No Improvement


Plasma
Bovine B
4 g/L
68
No Improvement


Gelatins
CW Fish Skin
1 g/L
67
No Improvement


Gelatins
CW Fish Skin
2 g/L
68
No Improvement


Gelatins
CW Fish Skin
4 g/L
71
No Improvement


Gelatins
Procine A (110 g)
1 g/L
74
No Improvement


Gelatins
Procine A (110 g)
2 g/L
83
No Improvement


Gelatins
Procine A (110 g)
4 g/L
69
No Improvement


Gelatins
Procine A (175 g)
1 g/L
72
No Improvement


Gelatins
Procine A (175 g)
2 g/L
76
No Improvement


Gelatins
Procine A (175 g)
4 g/L
67
No Improvement


Gelatins
Procine A (300 g)
1 g/L
67
No Improvement


Gelatins
Procine A (300 g)
2 g/L
66
No Improvement


Gelatins
Procine A (300 g)
4 g/L
65
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Chloride
5 mM
76
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
10 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
20 mM
77
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
30 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
40 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Chloride
50 mM
72
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
5 mM
77
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
10 mM
75
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
20 mM
78
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
30 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
40 mM
77
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Gluconate
50 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
5 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
20 mM
77
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
30 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
40 mM
78
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
50 mM
76
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Aldehydes
Formaldehyde Solution
0.010%
54
No Improvement


Aldehydes
Formaldehyde Solution
0.015%
52
No Improvement


Aldehydes
Formaldehyde Solution
0.020%
55
No Improvement


Aldehydes
Formaldehyde Solution
0.025%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.030%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.035%
54
No Improvement


Aldehydes
Formaldehyde Solution
0.040%
55
No Improvement


Aldehydes
Formaldehyde Solution
0.045%
54
No Improvement


Aldehydes
Formaldehyde Solution
0.050%
52
No Improvement


Aldehydes
Formaldehyde Solution
0.055%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.060%
56
No Improvement


Aldehydes
Formaldehyde Solution
0.065%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.070%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.075%
54
No Improvement


Aldehydes
Formaldehyde Solution
0.080%
55
No Improvement


Aldehydes
Formaldehyde Solution
0.085%
55
No Improvement


Aldehydes
Formaldehyde Solution
0.090%
54
No Improvement


Aldehydes
Formaldehyde Solution
0.095%
53
No Improvement


Aldehydes
Formaldehyde Solution
0.100%
53
No Improvement


Aldehydes
Paraformaldehyde Solution
0.010%
46
No Improvement


Aldehydes
Paraformaldehyde Solution
0.015%
39
No Improvement


Aldehydes
Paraformaldehyde Solution
0.020%
32
No Improvement


Aldehydes
Paraformaldehyde Solution
0.025%
33
No Improvement


Aldehydes
Paraformaldehyde Solution
0.030%
29
No Improvement


Aldehydes
Paraformaldehyde Solution
0.035%
27
No Improvement


Aldehydes
Paraformaldehyde Solution
0.040%
24
No Improvement


Aldehydes
Paraformaldehyde Solution
0.045%
21
No Improvement


Aldehydes
Paraformaldehyde Solution
0.050%
16
No Improvement


Aldehydes
Paraformaldehyde Solution
0.055%
15
No Improvement


Aldehydes
Paraformaldehyde Solution
0.060%
14
No Improvement


Aldehydes
Glutaraldehyde Solution
0.010%
59
No Improvement


Aldehydes
Glutaraldehyde Solution
0.015%
61
No Improvement


Aldehydes
Glutaraldehyde Solution
0.020%
62
No Improvement


Aldehydes
Glutaraldehyde Solution
0.025%
60
No Improvement


Aldehydes
Glutaraldehyde Solution
0.030%
56
No Improvement


Aldehydes
Glutaraldehyde Solution
0.035%
60
No Improvement


Aldehydes
Glutaraldehyde Solution
0.040%
61
No Improvement


Aldehydes
Glutaraldehyde Solution
0.045%
60
No Improvement


Aldehydes
Glutaraldehyde Solution
0.050%
57
No Improvement


Aldehydes
Glutaraldehyde Solution
0.055%
107 
Assay Interference


Aldehydes
Glutaraldehyde Solution
0.060%
102 
Assay Interference


Aldehydes
Glutaraldehyde Solution
0.065%
82
Assay Interference


Aldehydes
Glutaraldehyde Solution
0.070%
100 
Assay Interference


Aldehydes
Glutaraldehyde Solution
0.075%
53
No Improvement


Aldehydes
Glutaraldehyde Solution
0.080%
54
No Improvement


Aldehydes
Glutaraldehyde Solution
0.085%
54
No Improvement


Aldehydes
Glutaraldehyde Solution
0.090%
53
No Improvement


Aldehydes
Glutaraldehyde Solution
0.095%
51
No Improvement


Aldehydes
Glutaraldehyde Solution
0.100%
48
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
0.1% (50 g/mL, 100 mL/L,
66
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
0.25% (50 g/mL, 100 mL/L,
65
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
0.5% (50 g/mL, 100 mL/L,
63
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
0.75% (50 g/mL, 100 mL/L,
69
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
1% (50 g/mL, 100 mL/L,
64
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
2% (50 g/mL, 100 mL/L,
64
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
3% (50 g/mL, 100 mL/L,
63
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
4% (50 g/mL, 100 mL/L,
64
No Improvement




5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea + DMSO + Zinc Chloride
5% (50 g/mL, 100 mL/L,
59
No Improvement




5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
0.1% (50 g/mL, 100 mL/L,
70
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
0.25% (50 g/mL, 100 mL/L,
66
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
0.5% (50 g/mL, 100 mL/L,
69
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
0.75% (50 g/mL, 100 mL/L,
62
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
1% (50 g/mL, 100 mL/L,
70
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
2% (50 g/mL, 100 mL/L,
65
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
3% (50 g/mL, 100 mL/L,
66
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
4% (50 g/mL, 100 mL/L,
63
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea + DMSO + Zinc
5% (50 g/mL, 100 mL/L,
65
No Improvement



Chloride
5.8 g/L)




Formaldehyde Releaser
Diazolidinyl Urea
10 mM
60
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
25 mM
61
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
50 mM
49
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
75 mM
58
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
100 mM
50
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
10 mM
61
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
25 mM
62
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
50 mM
57
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
75 mM
55
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
100 mM
51
No Improvement


Formaldehyde Releaser
Soduim hyrdoxylmethyl glycinate
10 mM
48
No Improvement


Formaldehyde Releaser
Soduim hyrdoxylmethyl glycinate
25 mM
43
No Improvement


Formaldehyde Releaser
Soduim hyrdoxylmethyl glycinate
50 mM
74
No Improvement


Formaldehyde Releaser
Soduim hyrdoxylmethyl glycinate
75 mM
53
No Improvement


Formaldehyde Releaser
Soduim hyrdoxylmethyl glycinate
90 mM
36
No Improvement


Osmolytes
Betaine
0.05M
70
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
0.5M
70
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
1.25M
75
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
2.5M
73
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
3.75M
82
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
5M
80
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Trimethylamine N-oxide (TMANO)
0.043M
70
Slight Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
0.43M
70
Slight Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
1.1M
74
Slight Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
2.15M
72
Slight Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
3.2M
74
Slight Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
4.3M
68
Slight Improvement


Osmolytes
Betaine
2.5M
72
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
3M
73
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
3.5M
79
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
4M
78
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
4.5M
80
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Betaine
5M
83
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Trimethylamine N-oxide (TMANO)
2.5M
65
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
3M
64
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
3.5M
73
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
4M
69
No Improvement


Osmolytes
Trimethylamine N-oxide (TMANO)
4.3M
58
No Improvement


Detergent
Brij 35 (Component)
0.005%
63
No Improvement


Detergent
Brij 35 (Component)
0.010%
59
No Improvement


Detergent
Brij 35 (Component)
0.050%
69
No Improvement


Detergent
Brij 35 (Component)
0.100%
62
No Improvement


Detergent
Brij 35 (Component)
0.200%
57
No Improvement


Detergent
Brij 58 (Component)
0.005%
63
No Improvement


Detergent
Brij 58 (Component)
0.010%
67
No Improvement


Detergent
Brij 58 (Component)
0.050%
65
No Improvement


Detergent
Brij 58 (Component)
0.100%
63
No Improvement


Detergent
Brij 58 (Component)
0.200%
49
No Improvement


Detergent
Brij 35 (Spike)
0.010%
61
No Improvement


Detergent
Brij 35 (Spike)
0.050%
59
No Improvement


Detergent
Brij 35 (Spike)
0.080%
59
No Improvement


Detergent
Brij 35 (Spike)
0.100%
58
No Improvement


Detergent
Brij 58 (Spike)
0.010%
65
No Improvement


Detergent
Brij 58 (Spike)
0.050%
61
No Improvement


Detergent
Brij 58 (Spike)
0.080%
59
No Improvement


Detergent
Brij 58 (Spike)
0.100%
53
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
0.001M
62
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
0.01M
68
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
0.05M
16
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
0.001M + 2%
73
Slight Improvement with






Sugars. Needs further assay






optimization


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
0.01M + 2%
77
Slight Improvement with






Sugars. Needs further assay






optimization


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
0.05M + 2%
17
Slight Improvement with






Sugars. Needs further assay






optimization


Polyvalent Ions/Metal Salts
Magnesium Sulfate
0.001M
66
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
0.01M
75
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
0.05M
78
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
0.001M + 2%
72
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
0.01M + 2%
81
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
0.05M + 2%
80
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Copper Chloride
1 mM
64
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride
10 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride
25 mM
42
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Sucrose
1 mM + 2%
69
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Sucrose
10 mM + 2%
69
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Sucrose
25 mM + 2%
47
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride
1 mM
60
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride
5 mM
61
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride
10 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Sucrose
1 mM + 2%
65
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Sucrose
5 mM + 2%
65
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Sucrose
10 mM + 2%
59
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride
1 mM
59
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride
10 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride
25 mM
32
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride + Sucrose
1 mM + 2%
63
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride + Sucrose
10 mM + 2%
68
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride + Sucrose
25 mM + 2%
33
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride
1 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride
10 mM
61
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride
25 mM
33
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride + Sucrose
1 mM + 2%
68
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride + Sucrose
10 mM + 2%
75
No Improvement


Polyvalent Ions/Metal Salts
Zinc Chloride + Sucrose
25 mM + 2%
36
No Improvement


Polyvalent Ions/Metal Salts
Calcium Chloride
5 mM
66
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
65
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
20 mM
69
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
30 mM
59
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
40 mM
62
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride
50 mM
52
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
5 mM
62
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
10 mM
61
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
20 mM
70
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
30 mM
66
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
40 mM
62
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Propionate
50 mM
62
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Cobalt Chloride
5 mM + 5 mM
68
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Cobalt Chloride
10 mM + 5 mM
60
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Cobalt Chloride
5 mM + 10 mM
58
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Copper Chloride
5 mM + 5 mM
66
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Copper Chloride
10 mM + 5 mM
50
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Copper Chloride
5 mM + 10 mM
59
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Iron (3) Chloride
5 mM + 5 mM
76
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Iron (3) Chloride
5 mM + 2.5 mM
63
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Iron (3) Chloride
2.5 mM + 5 mM
63
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
5 mM + 5 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
10 mM + 5 mM
67
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
5 mM + 10 mM
61
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Nickel Chloride
5 mM + 5 mM
69
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Nickel Chloride
10 mM + 5 mM
64
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Nickel Chloride
5 mM + 10 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Zinc Chloride
5 mM + 5 mM
68
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Zinc Chloride
10 mM + 5 mM
66
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride + Zinc Chloride
5 mM + 10 mM
63
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Cobalt Chloride + Copper Chloride
5 mM + 5 mM
59
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Copper Chloride
10 mM + 5 mM
47
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Copper Chloride
5 mM + 10 mM
40
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Iron (3) Chloride
5 mM + 5 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Iron (3) Chloride
5 mM + 2.5 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Iron (3) Chloride
2.5 mM + 5 mM
65
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Magnesium Sulfate
5 mM + 5 mM
74
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Magnesium Sulfate
10 mM + 5 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Magnesium Sulfate
5 mM + 10 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Nickel Chloride
5 mM + 5 mM
75
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Nickel Chloride
10 mM + 5 mM
49
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Nickel Chloride
5 mM + 10 mM
43
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Zinc Chloride
5 mM + 5 mM
71
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Zinc Chloride
10 mM + 5 mM
35
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Zinc Chloride
5 mM + 10 mM
34
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Iron (3) Chloride
5 mM + 5 mM
67
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Iron (3) Chloride
5 mM + 2.5 mM
61
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Iron (3) Chloride
2.5 mM + 5 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Magnesium Sulfate
5 mM + 5 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Magnesium Sulfate
10 mM + 5 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Magnesium Sulfate
5 mM + 10 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Nickel Chloride
5 mM + 5 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Nickel Chloride
10 mM + 5 mM
42
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Nickel Chloride
5 mM + 10 mM
44
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Zinc Chloride
5 mM + 5 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Zinc Chloride
10 mM + 5 mM
38
No Improvement


Polyvalent Ions/Metal Salts
Copper Chloride + Zinc Chloride
5 mM + 10 mM
55
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Magnsium Sulfate
5 mM + 5 mM
71
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Magnsium Sulfate
5 mM + 2.5 mM
68
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Magnsium Sulfate
2.5 mM + 5 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Nickel Chloride
5 mM + 5 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Nickel Chloride
5 mM + 2.5 mM
58
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Nickel Chloride
2.5 mM + 5 mM
71
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Zinc Chloride
5 mM + 5 mM
61
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Zinc Chloride
5 mM + 2.5 mM
56
No Improvement


Polyvalent Ions/Metal Salts
Iron (3) Chloride + Zinc Chloride
2.5 mM + 5 mM
66
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Nickel Chloride
5 mM + 5 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Nickel Chloride
10 mM + 5 mM
68
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Nickel Chloride
5 mM + 10 mM
70
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Zinc Chloride
5 mM + 5 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Zinc Chloride
10 mM + 5 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Zinc Chloride
5 mM + 10 mM
74
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Nickel Chloride + Zinc Chloride
5 mM + 5 mM
74
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride + Zinc Chloride
10 mM + 5 mM
47
No Improvement


Polyvalent Ions/Metal Salts
Nickel Chloride + Zinc Chloride
5 mM + 10 mM
35
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
5%
82
Improvement in % Hb



(spike)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
10%
77
Improvement in % Hb



(spike)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
25%
76
Improvement in % Hb



(spike)


Recovery


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
5%
72
Slight Improvement. Needs



(spike)


further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
10%
78
Slight Improvement. Needs



(spike)


further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
25%
85
Slight Improvement. Needs



(spike)


further assay optimization


HRP Stabilizer Components
Fisher HRP Sol (spike)
5%
65
No Improvement


HRP Stabilizer Components
Fisher HRP Sol (spike)
10%
62
No Improvement


HRP Stabilizer Components
Fisher HRP Sol (spike)
25%
57
No Improvement


HRP Stabilizer Components
IC Tech (spike)
5%
64
No Improvement


HRP Stabilizer Components
IC Tech (spike)
10%
68
No Improvement


HRP Stabilizer Components
IC Tech (spike)
25%
65
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (spike)
5%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (spike)
10%
86
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (spike)
25%
82
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
1%
69
Improvement in % Hb



(component)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
2.50%
67
Improvement in % Hb



(component)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
5%
70
Improvement in % Hb



(component)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (comp)
1%
67
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 5Z02) (comp)
2.50%
69
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (comp)
5%
69
Improvement in % Hb






Recovery


HRP Stabilizer Components
Fisher HRP Sol (comp)
1%
65
No Improvement


HRP Stabilizer Components
Fisher HRP Sol (comp)
2.50%
65
No Improvement


HRP Stabilizer Components
Fisher HRP Sol (comp)
5%
64
No Improvement


HRP Stabilizer Components
ID Stabilization Buffer (comp)
1%
67
No Improvement


HRP Stabilizer Components
ID Stabilization Buffer (comp)
2.50%
66
No Improvement


HRP Stabilizer Components
ID Stabilization Buffer (comp)
5%
65
No Improvement


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
10%
74
Slight Improvement. Needs



(comp)


further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
25%
83
Slight Improvement. Needs



(comp)


further assay optimization


HRP Stabilizer Components
BioRad AbGuard HRP Stabilization Buffer
40%
90
Slight Improvement. Needs



(comp)


further assay optimization


HRP Stabilizer Components
IC Tech (comp)
1%
67
No Improvement


HRP Stabilizer Components
IC Tech (comp)
5%
67
No Improvement


HRP Stabilizer Components
IC Tech (comp)
10%
67
No Improvement


Polyvalent Ions/Metal Salts
Calcium Propionate + PEG 8000
0.1% + 3%
64
No improvement


Polyvalent Ions/Metal Salts
Calcium Propionate + PEG 8000
0.2% + 3%
63
No improvement


Polyvalent Ions/Metal Salts
Calcium Propionate + PEG 8000
0.3% + 3%
66
No improvement


Polyvalent Ions/Metal Salts
Calcium Propionate + Calcium Chloride +
0.1% + 3%
65
No improvement



PEG 8000





Polyvalent Ions/Metal Salts
Calcium Propionate + Calcium Chloride +
0.2% + 3%
68
No improvement



PEG 8000





Polyvalent Ions/Metal Salts
Calcium Propionate + Calcium Chloride +
0.3% + 3%
62
No improvement



PEG 8000





HRP Stabilizer Components
4-Bromophenol
0.001%
59
No Improvement


HRP Stabilizer Components
4-Bromophenol
0.005%
59
No Improvement


HRP Stabilizer Components
4-Bromophenol
0.01%
48
No Improvement


HRP Stabilizer Components
4-Bromophenol
0.05%
54
No Improvement


HRP Stabilizer Components
8-anilino-1-napthalene sulfonic acid
0.01%
61
No Improvement



(ANSA)





HRP Stabilizer Components
8-anilino-1-napthalene sulfonic acid
0.05%
68
No Improvement



(ANSA)





HRP Stabilizer Components
8-anilino-1-napthalene sulfonic acid
0.10%
59
No Improvement



(ANSA)





HRP Stabilizer Components
8-anilino-1-napthalene sulfonic acid
0.50%
42
No Improvement



(ANSA)





HRP Stabilizer Components
Proclin 300
0.00005%
61
No Improvement


HRP Stabilizer Components
Proclin 300
0.0001%
61
No Improvement


HRP Stabilizer Components
Proclin 300
0.0002%
55
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
0.1 mM
67
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
1 mM
71
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride
10 mM
63
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
0.1 mM + 2%
69
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
1 mM + 2%
69
No Improvement


Polyvalent Ions/Metal Salts
Cobalt Chloride + Sucrose
10 mM + 2%
74
No Improvement


Polyvalent Ions/Metal Salts
Magnesium Sulfate
0.1 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
1 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
56
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
0.1 mM + 2%
79
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
1 mM + 2%
68
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate + Sucrose
10 mM + 2%
71
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


HRP Stabilizer Components
Candor HRP Protector (spike)
25%
65
No Improvement


HRP Stabilizer Components
Candor HRP Protector (spike)
10%
61
No Improvement


HRP Stabilizer Components
Candor HRP Protector (spike)
5%
62
No Improvement


HRP Stabilizer Components
Innova LifeXtend Stabilizer (spike)
25%
62
No Improvement


HRP Stabilizer Components
Innova LifeXtend Stabilizer (spike)
10%
68
No Improvement


HRP Stabilizer Components
Innova LifeXtend Stabilizer (spike)
5%
63
No Improvement


Aldehydes
Acetaldehyde
10 mM
56
No Improvement


Aldehydes
Acetaldehyde
25 mM
48
No Improvement


Aldehydes
Acetaldehyde
50 mM
43
No Improvement


Aldehydes
Benzaldehyde
10 mM
46
No Improvement


Aldehydes
Benzaldehyde
25 mM
28
No Improvement


Aldehydes
Benzaldehyde
50 mM
53
No Improvement


Aldehydes
Butyraldehyde
10 mM
47
No Improvement


Aldehydes
Butyraldehyde
25 mM
34
No Improvement


Aldehydes
Butyraldehyde
50 mM
30
No Improvement


Aldehydes
Cinnamaldehyde
10 mM
25
No Improvement


Aldehydes
Cinnamaldehyde
25 mM
32
No Improvement


Aldehydes
Cinnamaldehyde
50 mM
21
No Improvement


Aldehydes
Formamide
10 mM
62
No Improvement


Aldehydes
Formamide
25 mM
62
No Improvement


Aldehydes
Formamide
50 mM
62
No Improvement


Aldehydes
Furfural
10 mM
58
No Improvement


Aldehydes
Furfural
25 mM
37
No Improvement


Aldehydes
Furfural
50 mM
38
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
10 mM
61
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
25 mM
33
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
50 mM
16
No Improvement


Aldehydes
Glycolaldehyde Dimer
10 mM
37
No Improvement


Aldehydes
Glycolaldehyde Dimer
25 mM
11
No Improvement


Aldehydes
Glycolaldehyde Dimer
50 mM
2
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
10 mM
57
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
25 mM
44
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
50 mM
48
No Improvement


Aldehydes
p-Tolualdehyde
10 mM
51
No Improvement


Aldehydes
p-Tolualdehyde
25 mM
35
No Improvement


Aldehydes
p-Tolualdehyde
50 mM
71
No Improvement


Aldehydes
Vanillin
10 mM
40
No Improvement


Aldehydes
Vanillin
25 mM
32
No Improvement


Aldehydes
Vanillin
50 mM
34
No Improvement


Aldehydes
Acetaldehyde
0.01 mM
55
No Improvement


Aldehydes
Acetaldehyde
0.1 mM
62
No Improvement


Aldehydes
Acetaldehyde
1 mM
66
No Improvement


Aldehydes
Acetaldehyde
10 mM
56
No Improvement


Aldehydes
Acetaldehyde
25 mM
48
No Improvement


Aldehydes
Acetaldehyde
50 mM
43
No Improvement


Aldehydes
Benzaldehyde
0.01 mM
55
No Improvement


Aldehydes
Benzaldehyde
0.1 mM
50
No Improvement


Aldehydes
Benzaldehyde
1 mM
19
No Improvement


Aldehydes
Benzaldehyde
10 mM
46
No Improvement


Aldehydes
Benzaldehyde
25 mM
28
No Improvement


Aldehydes
Benzaldehyde
50 mM
53
No Improvement


Aldehydes
Butyraldehyde
0.01 mM
60
No Improvement


Aldehydes
Butyraldehyde
0.1 mM
57
No Improvement


Aldehydes
Butyraldehyde
1 mM
29
No Improvement


Aldehydes
Butyraldehyde
10 mM
47
No Improvement


Aldehydes
Butyraldehyde
25 mM
34
No Improvement


Aldehydes
Butyraldehyde
50 mM
30
No Improvement


Aldehydes
Cinnamaldehyde
0.01 mM
49
No Improvement


Aldehydes
Cinnamaldehyde
0.1 mM
53
No Improvement


Aldehydes
Cinnamaldehyde
1 mM
25
No Improvement


Aldehydes
Cinnamaldehyde
10 mM
25
No Improvement


Aldehydes
Cinnamaldehyde
25 mM
32
No Improvement


Aldehydes
Cinnamaldehyde
50 mM
21
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
0.01 mM
66
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
0.1 mM
60
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
1 mM
30
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
10 mM
61
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
25 mM
33
No Improvement


Aldehydes
D-( + )-Glyceraldehyde
50 mM
16
No Improvement


Aldehydes
Glycolaldehyde Dimer
0.01 mM
61
No Improvement


Aldehydes
Glycolaldehyde Dimer
0.1 mM
68
No Improvement


Aldehydes
Glycolaldehyde Dimer
1 mM
50
No Improvement


Aldehydes
Glycolaldehyde Dimer
10 mM
37
No Improvement


Aldehydes
Glycolaldehyde Dimer
25 mM
11
No Improvement


Aldehydes
Glycolaldehyde Dimer
50 mM
2
No Improvement


Aldehydes
Formamide
0.01 mM
58
No Improvement


Aldehydes
Formamide
0.1 mM
67
No Improvement


Aldehydes
Formamide
1 mM
69
No Improvement


Aldehydes
Formamide
10 mM
62
No Improvement


Aldehydes
Formamide
25 mM
62
No Improvement


Aldehydes
Formamide
50 mM
62
No Improvement


Aldehydes
Furfural
0.01 mM
63
No Improvement


Aldehydes
Furfural
0.1 mM
65
No Improvement


Aldehydes
Furfural
1 mM
34
No Improvement


Aldehydes
Furfural
10 mM
58
No Improvement


Aldehydes
Furfural
25 mM
37
No Improvement


Aldehydes
Furfural
50 mM
38
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
0.01 mM
69
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
0.1 mM
61
No Improvement


Aldehydes
4-sobutylbenzaldehyde
1 mM
31
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
10 mM
57
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
25 mM
44
No Improvement


Aldehydes
4-Isobutylbenzaldehyde
50 mM
48
No Improvement


Aldehydes
p-Tolualdehyde
0.01 mM
65
No Improvement


Aldehydes
p-Tolualdehyde
0.1 mM
62
No Improvement


Aldehydes
p-Tolualdehyde
1 mM
35
No Improvement


Aldehydes
p-Tolualdehyde
10 mM
51
No Improvement


Aldehydes
p-Tolualdehyde
25 mM
35
No Improvement


Aldehydes
p-Tolualdehyde
50 mM
71
No Improvement


Aldehydes
Vanillin
0.01 mM
66
No Improvement


Aldehydes
Vanillin
0.1 mM
58
No Improvement


Aldehydes
Vanillin
1 mM
32
No Improvement


Aldehydes
Vanillin
10 mM
40
No Improvement


Aldehydes
Vanillin
25 mM
32
No Improvement


Aldehydes
Vanillin
50 mM
34
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
5%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
10%
80
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
25%
77
Improvement in % Hb






Recovery


Formaldehyde Releaser
Bronidox
5 mM
47
No Improvement


Formaldehyde Releaser
Bronidox
10 mM
27
No Improvement


Formaldehyde Releaser
Bronidox
25 mM
37
No Improvement


Formaldehyde Releaser
Bronopol
5 mM
34
No Improvement


Formaldehyde Releaser
Bronopol
10 mM
31
No Improvement


Formaldehyde Releaser
Bronopol
25 mM
24
No Improvement


Preservatives/Parabens
Benzyl 4-hydroxybenzoate
5 mM
63
No Improvement


Preservatives/Parabens
Benzyl 4-hydroxybenzoate
10 mM
35
No Improvement


Preservatives/Parabens
Benzyl 4-hydroxybenzoate
25 mM
26
No Improvement


Preservatives/Parabens
Ethyl 4-hydroxybenzoate
5 mM
54
No Improvement


Preservatives/Parabens
Ethyl 4-hydroxybenzoate
10 mM
43
No Improvement


Preservatives/Parabens
Ethyl 4-hydroxybenzoate
25 mM
30
No Improvement


Preservatives/Parabens
Methyl 4-hydroxybenzoate
5 mM
58
No Improvement


Preservatives/Parabens
Methyl 4-hydroxybenzoate
10 mM
44
No Improvement


Preservatives/Parabens
Methyl 4-hydroxybenzoate
25 mM
34
No Improvement


Preservatives/Parabens
Propyl 4-hydroxybenzoate
5 mM
55
No Improvement


Preservatives/Parabens
Propyl 4-hydroxybenzoate
10 mM
37
No Improvement


Preservatives/Parabens
Propyl 4-hydroxybenzoate
25 mM
28
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
1%
69
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
5%
79
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
10%
76
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
81
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
20%
75
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
25%
76
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
30%
70
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
40%
71
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Aluminum Chloride
2.5 mM
69
No Improvement


Polyvalent Ions/Metal Salts
Aluminum Chloride
5 mM
70
No Improvement


Polyvalent Ions/Metal Salts
Aluminum Chloride
10 mM
29
No Improvement


Formaldehyde Releaser
Quaternium 15
5 mM
52
No Improvement


Formaldehyde Releaser
Quaternium 15
10 mM
47
No Improvement


Formaldehyde Releaser
Quaternium 15
20 mM
50
No Improvement


Preservatives
Sodium Nitrate
5 mM
70
No Improvement


Preservatives
Sodium Nitrate
10 mM
65
No Improvement


Preservatives
Sodium Nitrate
20 mM
56
No Improvement


Preservatives
Sodium Nitrite
5 mM
64
No Improvement


Preservatives
Sodium Nitrite
10 mM
65
No Improvement


Preservatives
Sodium Nitrite
20 mM
62
No Improvement


Substrates
Styrene Glycol
5 mM
56
No Improvement


Substrates
Styrene Glycol
10 mM
58
No Improvement


Substrates
Styrene Glycol
20 mM
48
No Improvement


Substrates
Styrene Oxide
5 mM
57
No Improvement


Substrates
Styrene Oxide
10 mM
42
No Improvement


Substrates
Styrene Oxide
20 mM
31
No Improvement


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
0.1 mM
52
No Improvement


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
1 mM
63
No Improvement


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
10 mM
35
No Improvement


Polyvalent Ions/Metal Salts
Sodium Citrate
0.1 mM
61
No Improvement


Polyvalent Ions/Metal Salts
Sodium Citrate
1 mM
60
No Improvement


Polyvalent Ions/Metal Salts
Sodium Citrate
10 mM
55
No Improvement


Vitamins
Vitamin B2
0.002 mM
58
No Improvement


Vitamins
Vitamin B2
0.02 mM
48
No Improvement


Vitamins
Vitamin B2
0.2 mM
45
No Improvement


Vitamins
Vitamin B3
0.1 mM
53
No Improvement


Vitamins
Vitamin B3
1 mM
58
No Improvement


Vitamins
Vitamin B3
10 mM
42
No Improvement


Vitamins
Vitamin B4
0.1 mM
54
No Improvement


Vitamins
Vitamin B4
1 mM
57
No Improvement


Vitamins
Vitamin B4
10 mM
27
No Improvement


Vitamins
Vitamin B9
0.1 mM
58
No Improvement


Vitamins
Vitamin B9
1 mM
43
No Improvement


Vitamins
Vitamin B9
10 mM
37
No Improvement


Vitamins
NAD +
0.05 mM
59
No Improvement


Vitamins
NAD +
0.5 mM
54
No Improvement


Vitamins
NAD +
5 mM
57
No Improvement


Vitamins
NADH
0.1 mM
63
No Improvement


Vitamins
NADH
1 mM
66
No Improvement


Vitamins
NADH
10 mM
65
No Improvement


Vitamins
Vitamin B12
0.01 mM
53
No Improvement


Vitamins
Vitamin B12
0.1 mM
62
No Improvement


Vitamins
Vitamin B12
1 mM
59
No Improvement


Vitamins
Vitamin B6
0.1 mM
55
No Improvement


Vitamins
Vitamin B6
1 mM
53
No Improvement


Vitamins
Vitamin B6
10 mM
58
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
10%
77
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
74
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
20%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
25%
74
Improvement in % Hb






Recovery


Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
10%
67
No Improvement



Proclin 300 (10 mM MOPS)





Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
15%
62
No Improvement



Proclin 300 (10 mM MOPS)





Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
20%
66
No Improvement



Proclin 300 (10 mM MOPS)





Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
25%
61
No Improvement



Proclin 300 (10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
0.002%, 0.002%, 0.0002%
64
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
0.003%, 0.003%, 0.0003%
57
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
0.004%, 0.004%, 0.0004%
52
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
0.005%, 0.005%, 0.0005%
45
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone
0.002%, 0.002%
64
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone
0.003%, 0.003%
58
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone
0.004%, 0.004%
56
No Improvement



(10 mM MOPS)





Formaldehyde Releaser
Bronidox, Methylisothiazolone
0.005%, 0.005%
53
No Improvement



(10 mM MOPS)





HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
10%
75
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
72
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
20%
75
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
25%
63
Improvement in % Hb






Recovery


Formaldehyde Releaser
Bronidox
0.002%
67
No Improvement


Formaldehyde Releaser
Bronidox
0.003%
65
No Improvement


Formaldehyde Releaser
Bronidox
0.004%
69
No Improvement


Formaldehyde Releaser
Bronidox
0.005%
65
No Improvement


Enzyme Inhibitor
Methylisothiazolone
0.002%
54
No Improvement


Enzyme Inhibitor
Methylisothiazolone
0.003%
58
No Improvement


Enzyme Inhibitor
Methylisothiazolone
0.004%
56
No Improvement


Enzyme Inhibitor
Methylisothiazolone
0.005%
47
No Improvement


HRP Stabilizer Components
Proclin 300
0.0002%
68
No Improvement


HRP Stabilizer Components
Proclin 300
0.0003%
68
No Improvement


HRP Stabilizer Components
Proclin 300
0.0004%
69
No Improvement


HRP Stabilizer Components
Proclin 300
0.0005%
63
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
10%
81
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
74
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
20%
70
Improvement in % Hb






Recovery


Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
10% (1%, 0.02%, 0.02%,
47
No Improvement



Proclin 300 (10 mM MOPS)
0.002%)




Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
15% (1%, 0.02%, 0.02%,
43
No Improvement



Proclin 300 (10 mM MOPS)
0.002%)




Formaldehyde Releaser
BSA, Bronidox, Methylisothiazolone,
20% (1%, 0.02%, 0.02%,
39
No Improvement



Proclin 300 (10 mM MOPS)
0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
10% (0.02%, 0.02%,
52
No Improvement



(10mM MOPS)
0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
15% (0.02%, 0.02%,
42
No Improvement



(10mM MOPS)
0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
20% (0.02%, 0.02%,
37
No Improvement



(10mM MOPS)
0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
10% (0.02%, 0.02%,
56
No Improvement




0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
15% (0.02%, 0.02%,
41
No Improvement




0.002%)




Formaldehyde Releaser
Bronidox, Methylisothiazolone, Prolin 300
20% (0.02%, 0.02%,
45
No Improvement




0.002%)




Plasma
BSA (10 mM MOPS)
1%
76
No Improvement


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
15%
76
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
87
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
89
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 4%
76
Improvement in % Hb



RD Hb Collection Buffer


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15%
75
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15% + 10 mM
87
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRPConjugate Stabilizer (PN SZ02)
15% + 10 mM
92
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) + RD
15% + 4%
77
Improvement in % Hb



Hb Collection Buffer


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
77
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
92
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
95
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 4%
77
Improvement in % Hb



RD Hb Collection Buffer


Recovery


HRP Stabilizer Components
DEAE Dextran, Ethylene Glycol, Calcium
0.5%, 10%, 10 mM
63
No Improvement



Chloride





HRP Stabilizer Components
L.s. Dextran, Ethylene Glycol, Calcium
0.5%, 10%, 10 mM
52
No Improvement



Chloride





Sugars/Saccharides
Sucrose
0.5M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
1M
91
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
2M
129 
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.125M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.25M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.5M
87
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Glucose
0.25M + 0.25M
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Glucose
0.5M + 0.5M
93
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Glucose
1M + 1M
100 
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Trehalose
0.25M + 0.125M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Trehalose
0.5M + 0.25M
75
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Trehalose
1M + 0.5M
141 
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Raffinose
0.25M + 0.0625M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Raffinose
0.5M + 0.125M
85
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Raffinose
1M + 0.25M
104 
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Trehalose
0.0625M + 0.125M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Trehalose
0.125M + 0.25M
88
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Trehalose
0.25M + 0.5M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Glucose
0.0625M + 0.25M
70
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Glucose
0.125M + 0.5M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Glucose
0.25M + 1M
90
Slight Improvement with






Sugars. Needs further assay






optimization


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
5%
66
No improvement


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
10%
61
No improvement


Enzyme Inhibitor
aurintircarboxylic acid ammonium salt
25%
59
No improvement


Sugars/Saccharides
Raffinose
0.05M
69
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.1M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.15M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.2M
80
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.25M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.3M
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.35M
80
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.4M
89
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.45M
69
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.5M
91
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.03M
67
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.06M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.09M
68
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.12M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.15M
75
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.18M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.21M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.24M
82
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.27M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.3M
85
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose
0.2M
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Betaine
0.2M + 0.125M
83
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Betaine
0.2M + 0.25M
80
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Betaine
0.2M + 0.5M
85
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Calcium Chloride
0.2M + 1 mM
88
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Calcium Chloride
0.2M + 5 mM
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Calcium Chloride
0.2M + 10 mM
90
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Ectoine
0.2M + 75 mM
88
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Ectoine
0.2M + 150 mM
81
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Ectoine
0.2M + 300 mM
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Magnesium Sulfate
0.2M + 1 mM
90
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Magnesium Sulfate
0.2M + 5 mM
91
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + Magnesium Sulfate
0.2M + 10 mM
87
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + TMANO
0.2M + 0.125 M
81
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + TMANO
0.2M + 0.25 M
98
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Raffinose + TMANO
0.2M + 0.5M
84
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose
0.24M
61
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Betaine
0.24M + 0.125M
87
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Betaine
0.24M + 0.245M
84
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Betaine
0.24M + 0.5M
84
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Calcium Chloride
0.24M + 1 mM
83
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Calcium Chloride
0.24M + 5 mM
87
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Calcium Chloride
0.24M + 10 mM
94
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Ectoine
0.24M + 75 mM
84
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Ectoine
0.24M + 150 mM
85
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Ectoine
0.24M + 300 mM
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Magnesium Sulfate
0.24M + 1 mM
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Magnesium Sulfate
0.24M + 5 mM
89
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + Magnesium Sulfate
0.24M + 10 mM
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + TMANO
0.24M + 0.125M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + TMANO
0.24M + 0.245M
87
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Sucrose + TMANO
0.24M + 0.5M
79
Slight Improvement with






Sugars. Needs further assay






optimization


HRP Stabilizer Components
Phenol Red
0.01%
70
No improvement


HRP Stabilizer Components
Phenol Red
0.03%
71
No improvement


HRP Stabilizer Components
Phenol Red
0.05%
70
No improvement


HRP Stabilizer Components
Sodium Benzoate
0.10%
67
No improvement


HRP Stabilizer Components
Sodium Benzoate
0.50%
60
No improvement


HRP Stabilizer Components
Sodium Benzoate
1%
54
No improvement


HRP Stabilizer Components
Fisher HRP Sol
5%
71
No improvement


HRP Stabilizer Components
Fisher HRP Sol
10%
70
No improvement


HRP Stabilizer Components
Fisher HRP Sol
25%
68
No improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (comp)
5%
83
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) (spike)
5%
71
Improvement in % Hb






Recovery


Formaldehyde Releaser
Bronidox
0.0002%
69
No improvement


Formaldehyde Releaser
Bronidox
0.0010%
68
No improvement


Formaldehyde Releaser
Bronidox
0.0020%
70
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.0002%
71
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.0010%
68
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.0020%
63
No improvement


Flavoniods
7,8 dihydroxyflavone
0.25 mM
51
No improvement


Flavoniods
7,8 dihydroxyflavone
0.5 mM
39
No improvement


Flavoniods
7,8 dihydroxyflavone
1 mM
38
No improvement


Flavoniods
Quercetin
150 uM
45
No improvement


Flavoniods
Quercetin
300 uM
38
No improvement


Flavoniods
Quercetin
600 uM
35
No improvement


Flavoniods
Diadzein
25 uM
68
No improvement


Flavoniods
Diadzein
50 uM
78
No improvement


Flavoniods
Diadzein
100 uM
68
No improvement


Redox/antioxidants
Ascorbic Acid
0.5 mM
65
No improvement


Redox/antioxidants
Ascorbic Acid
1 mM
67
No improvement


Redox/antioxidants
Ascorbic Acid
5 mM
57
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.00005%
68
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.0001%
68
No improvement


Enzyme Inhibitor
Methylisothiazolone
0.0002%
67
No improvement


Preservatives
Sodium Benzoate
0.03%
69
No improvement


Preservatives
Sodium Benzoate
0.05%
73
No improvement


Preservatives
Sodium Benzoate
0.10%
65
No improvement


Flavoniods
Diadzein
40 mM
66
No improvement


Flavoniods
Diadzein
50 mM
65
No improvement


Flavoniods
Diadzein
60 mM
66
No improvement


Substrates
Resveratrol
5 mM
48
No improvement


Substrates
Resveratrol
10 mM
40
No improvement


Substrates
Resveratrol
20 mM
38
No improvement


Preservatives
Sodium Benzoate
0.04%
67
No improvement


Preservatives
Sodium Benzoate
0.05%
68
No improvement


Preservatives
Sodium Benzoate
0.06%
67
No improvement


Vitamins
Tocopherol
0.10%
71
No improvement


Vitamins
Tocopherol
1%
73
No improvement


Vitamins
Tocopherol
10%
65
No improvement


Vitamins
Vitamin B6
0.5 mM
68
No improvement


Vitamins
Vitamin B6
1 mM
67
No improvement


Vitamins
Vitamin B6
2 mM
66
No improvement


Vitamins
Vitamine B6 in HCl
0.5 mM
68
No improvement


Vitamins
Vitamine B6 in HCl
1 mM
41
No improvement


Vitamins
Vitamine B6 in HCl
2 mM
40
No improvement


Plasma
Fetal Bovine Serum
1%
58
No improvement


Plasma
Fetal Bovine Serum
10%
63
No improvement


Plasma
Fetal Bovine Serum
50%
68
No improvement


Plasma
Gamma Globulins
10 mg/mL
85
No improvement


Plasma
Gamma Globulins
25 mg/mL
89
No improvement


Plasma
Gamma Globulins
50 mg/mL
88
No improvement


Sugars/Saccharides
Glucose
0.2M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
0.4M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
0.6M
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
0.8M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
1.0M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
1.2M
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
1.4M
80
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
1.6M
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
1.8M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
2M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.1M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.2M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.3M
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.4M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.5M
82
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.6M
82
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.7M
83
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.8M
82
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.9M
85
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
1M
86
Slight Improvement with






Sugars. Needs further assay






optimization


Osmolytes
Betaine
1M
77
Betaine shows improvement






in % Hb Recovery at high






concentrations


Osmolytes
Ectoine
0.3M
65
No improvement


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
78
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


Osmolytes
Trimethylamine N-oxide (TMANO)
1M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose
0.6M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
0.6M + 0.125M
74
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
0.6M + 0.25M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Betaine
0.6M + 0.5M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Calcium Chloride
0.6M + 1 mM
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Calcium Chloride
0.6M + 5 mM
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Calcium Chloride
0.6M + 10 mM
84
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Ectoine
0.6M + 0.075M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Ectoine
0.6M + 0.15M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Ectoine
0.6M + 0.3M
71
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Magnesium Sulfate
0.6M + 1 mM
75
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Magnesium Sulfate
0.6M + 5 mM
75
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + Magnesium Sulfate
0.6M + 10 mM
78
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + TMANO
0.6M + 0.125M
69
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + TMANO
0.6M + 0.25M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Glucose + TMANO
0.6M + 0.5M
68
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose
0.3M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Betaine
0.3M + 0.125M
73
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Betaine
0.3M + 0.25M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Betaine
0.3M + 0.5M
81
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Calcium Chloride
0.3M + 1 mM
69
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Calcium Chloride
0.3M + 5 mM
68
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Calcium Chloride
0.3M + 10 mM
83
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Ectoine
0.3M + 0.075M
77
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Ectoine
0.3M + 0.15M
72
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Ectoine
0.3M + 0.3M
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Magnesium Sulfate
0.3M + 1 mM
68
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Magnesium Sulfate
0.3M + 5 mM
80
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + Magnesium Sulfate
0.3M + 10 mM
76
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + TMANO
0.3M + 0.125M
70
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + TMANO
0.3M + 0.25M
79
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Trehalose + TMANO
0.3M + 0.5M
75
Slight Improvement with






Sugars. Needs further assay






optimization


Sugars/Saccharides
Erlose
1 mM
72
No improvement


Sugars/Saccharides
Erlose
5 mM
69
No improvement


Sugars/Saccharides
Erlose
10 mM
76
No improvement


Sugars/Saccharides
Fructose
0.05M
67
No improvement


Sugars/Saccharides
Fructose
0.1M
69
No improvement


Sugars/Saccharides
Fructose
0.25M
65
No improvement


Sugars/Saccharides
Galactose
0.1M
71
No improvement


Sugars/Saccharides
Galactose
0.25M
68
No improvement


Sugars/Saccharides
Galactose
0.5M
76
No improvement


Sugars/Saccharides
Kestose
1 mM
71
No improvement


Sugars/Saccharides
Kestose
5 mM
68
No improvement


Sugars/Saccharides
Kestose
10 mM
76
No improvement


Sugars/Saccharides
Lactose
0.025M
66
No Improvement


Sugars/Saccharides
Lactose
0.05M
71
No Improvement


Sugars/Saccharides
Lactose
0.1M
65
No Improvement


Sugars/Saccharides
Maltose
0.1M
72
No Improvement


Sugars/Saccharides
Maltose
0.25M
69
No Improvement


Sugars/Saccharides
Maltose
0.5M
76
No Improvement


Sugars/Saccharides
Melezitose
10 mM
66
No Improvement


Sugars/Saccharides
Melezitose
50 mM
71
No Improvement


Sugars/Saccharides
Melezitose
100 mM
65
No Improvement


Sugars/Saccharides
Stachyose
1 mM
67
No Improvement


Sugars/Saccharides
Stachyose
5 mM
69
No Improvement


Sugars/Saccharides
Stachyose
7 mM
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
1% (325 g/L, 90 g/L, 40 g/L)
66
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
1% (500 g/L, 90 g/L, 40 g/L)
63
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
1% (325 g/L, 500 g/L,
62
No Improvement



EDTA, Glycine
90 g/L, 40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
5% (325 g/L, 90 g/L, 40 g/L)
50
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
5% (500 g/L, 90 g/L, 40 g/L)
56
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
5% (325 g/L, 500 g/L,
48
No Improvement



EDTA, Glycine
90 g/L, 40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
10% (325 g/L, 90 g/L, 40 g/L)
43
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
10% (500 g/L, 90 g/L, 40 g/L)
45
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
10% (325 g/L, 500 g/L,
35
No Improvement



EDTA, Glycine
90 g/L, 40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
1% (1.25%, 2.05%, 0.16%)
64
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
1% (2.10%, 2.05%, 0.16%)
71
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
1% (1.25%, 2.10%, 2.05%,
67
No Improvement



EDTA, Glycine
0.16%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
10% (1.25%, 2.05%, 0.16%)
65
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
10% (2.10%, 2.05%, 0.16%)
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
10% (1.25%, 2.10%, 2.05%,
64
No Improvement



EDTA, Glycine
0.16%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
20% (1.25%, 2.05%, 0.16%)
58
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
20% (2.10%, 2.05%, 0.16%)
63
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
20% (1.25%, 2.10%, 2.05%,
60
NoImprovement



EDTA, Glycine
0.16%)




Formaldehyde Releaser
Diazolidinyl Urea
400 g/L
 6
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
200 g/L
20
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
100 g/L
39
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
50 g/L
55
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
25 g/L
58
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
12.5 g/L
62
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
400 g/L
14
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
200 g/L
32
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
100 g/L
45
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
50 g/L
55
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
25 g/L
57
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
12.5 g/L
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
10% (325 g/L, 90 g/L, 40 g/L)
40
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine
1% (325 g/L, 90 g/L, 40 g/L)
67
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
10% (500 g/L, 90 g/L, 40 g/L)
51
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea, EDTA, Glycine
1% (500 g/L, 90g/L, 40 g/L)
67
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
10% (325 g/L, 500 g/L,
42
No Improvement



EDTA, Glycine
90 g/L, 40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, Imidazolidinyl Urea,
1% (325 g/L, 500 g/L,
64
No Improvement



EDTA, Glycine
90 g/L, 40 g/L)




Formaldehyde Releaser
Imidazolidinyl Urea, pH 6.5 (RT)
0.1%
66
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Glycine, pH 6.5 (RT)
0.1% + 0.1%
67
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Urea, pH 6.5 (RT)
0.1% + 0.1%
64
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Ammonium
0.1% + 0.1%
67
No Improvement



Chloride, pH 6.5 (RT)





Formaldehyde Releaser
Imidazolidinyl Urea, pH 7.0 (RT)
0.1%
64
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Glycine, pH 7.0 (RT)
0.1% + 0.1%
65
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Urea, pH 7.0 (RT)
0.1% + 0.1%
66
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Ammonium
0.1% + 0.1%
67
No Improvement



Chloride, pH 7.0 (RT)





Formaldehyde Releaser
Imidazolidinyl Urea, pH 7.5 (RT)
0.1%
64
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Glycine, pH 7.5 (RT)
0.1% + 0.1%
66
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Urea, pH 7.5 (RT)
0.1% + 0.1%
62
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea + Ammonium
0.1% + 0.1%
67
No Improvement



Chloride, pH 7.5 (RT)





Formaldehyde Releaser
Diazolidinyl Urea, pH 6.5 (RT)
0.1%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 6.5 (RT)
0.1% + 0.1%
66
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Urea, pH 6.5 (RT)
0.1% + 0.1%
62
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Ammonium Chloride,
0.1% + 0.1%
66
No Improvement



pH 6.5 (RT)





Formaldehyde Releaser
Diazolidinyl Urea, pH 7.0 (RT)
0.1%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.0 (RT)
0.1% + 0.1%
66
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Urea, pH 7.0 (RT)
0.1% + 0.1%
64
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Ammonium Chloride,
0.1% + 0.1%
66
No Improvement



pH 7.0 (RT)





Formaldehyde Releaser
Diazolidinyl Urea, pH 7.5 (RT)
0.1%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5 (RT)
0.1% + 0.1%
66
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Urea, pH 7.5 (RT)
0.1% + 0.1%
63
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Ammonium Chloride,
0.1% + 0.1%
66
No Improvement



pH 7.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1%
59
No Improvement



azoniaadamantane chloride Urea,






pH 6.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
59
No Improvement



azoniaadamantane chloride Urea + Glycine,






pH 6.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
58
No Improvement



azoniaadamantane chloride Urea + Urea,






pH 6.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
59
No Improvement



azoniaadamantane chloride Urea +






Ammonium Chloride, pH 6.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1%
56
No Improvement



azoniaadamantane chloride Urea,






pH 7.0 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
59
No Improvement



azoniaadamantane chloride Urea +






Glycine, pH 7.0 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
57
No Improvement



azoniaadamantane chloride Urea + Urea,






pH 7.0 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
59
No Improvement



azoniaadamantane chloride Urea +






Ammonium Chloride, pH 7.0 (RT)





Formaldehyde Releaser
1-(cis-3-ChloroallyI)-3,5,7-triaza-1-
0.1%
58
No Improvement



azoniaadamantane chloride Urea,






pH 7.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
57
No Improvement



azoniaadamantane chloride Urea +






Glycine, pH 7.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
55
No Improvement



azoniaadamantane chloride Urea + Urea,






pH 7.5 (RT)





Formaldehyde Releaser
1-(cis-3-Chloroallyl)-3,5,7-triaza-1-
0.1% + 0.1%
58
No Improvement



azoniaadamantane chloride Urea +






Ammonium Chloride, pH 7.5 (RT)





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5 (50 C.)
0.1% + 0.1%
62
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.1% + 0.1% + 0.2%
42
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.1% + 0.1% + 0.1%
43
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.1% + 0.1% + 0.05%
41
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5 (50 C.)
0.3% + 0.3%
64
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.3% + 0.3% + 0.6%
42
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.3% + 0.3% + 0.3%
33
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Diazolidinyl Urea + Glycine, pH 7.5
0.3% + 0.3% + 0.15%
35
No Improvement



(50 C.) + methylisothiazolone





Formaldehyde Releaser
Bronopol + ATA, pH 6.5
0.002% + 0.002%
72
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 7.5
0.002% + 0.002%
68
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 8.5
0.020% + 0.002%
70
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 6.5
0.001% + 0.002%
71
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 7.5
0.001% + 0.002%
67
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 8.5
0.001% + 0.002%
68
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 6.5
0.004% + 0.002%
71
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 7.5
0.004% + 0.002%
71
No Improvement


Formaldehyde Releaser
Bronopol + ATA, pH 8.5
0.004% + 0.002%
72
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 6.5
0.002% + 0.002%
63
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 7.5
0.002% + 0.002%
65
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 8.5
0.002% + 0.002%
59
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 6.5
0.001% + 0.002%
62
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 7.5
0.001% + 0.002%
65
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 8.5
0.001% + 0.002%
64
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 6.5
0.004% + 0.002%
65
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 7.5
0.004% + 0.002%
66
No Improvement


Formaldehyde Releaser
Bronopol + Methylisothiazolone, pH 8.5
0.004% + 0.002%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 6.5
0.002% + 0.002%
71
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 7.5
0.002% + 0.002%
66
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 8.5
0.002% + 0.002%
69
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 6.5
0.001% + 0.002%
69
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 7.5
0.001% + 0.002%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 8.5
0.001% + 0.002%
73
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 6.5
0.004% + 0.002%
69
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 7.5
0.004% + 0.002%
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + ATA, pH 8.5
0.004% + 0.002%
70
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.002% + 0.002%
58
No Improvement



pH 6.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.002% + 0.002%
58
No Improvement



pH 7.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.002% + 0.002%
60
No Improvement



pH 8.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.001% + 0.002%
62
No Improvement



pH 6.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.001% + 0.002%
62
No Improvement



pH 7.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.001% + 0.002%
58
No Improvement



pH 8.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.004% + 0.002%
60
No Improvement



pH 6.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.004% + 0.002%
62
No Improvement



pH 7.5





Formaldehyde Releaser
Diazolidinyl Urea + Methylisothiazolone,
0.004% + 0.002%
59
No Improvement



pH 8.5





Aldehydes
Formaldehyde
0.10%
58
No Improvement


Aldehydes
Formaldehyde
0.50%
54
No Improvement


Aldehydes
Formaldehyde
1%
25
No Improvement


Aldehydes
Formaldehyde
2%
 5
No Improvement


Aldehydes
Formaldehyde
3%
 4
No Improvement


Aldehydes
Formaldehyde
4%
 4
No Improvement


Aldehydes
Formaldehyde
5%
 6
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
93
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
99
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
10%
84
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15%
85
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
20%
84
Improvement in % Hb






Recovery


HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
10% (1%, 0.02%, 0.02%,
53
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
15% (1%, 0.02%, 0.02%,
51
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
20% (1%, 0.02%, 0.02%,
54
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
10% (1%, 0.02%, 0.02%,
59
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
15% (1%, 0.02%, 0.02%,
61
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
20% (1%, 0.02%, 0.02%,
49
No Improvement



isothiazolone, Proclin 300 in 10 mM
0.002%)





MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
10% (1%, 0.02%, 0.02%,
54
No Improvement



isothiazolone, Proclin 300 Calcium
0.002%, 10 mM)





Chloride in 10 mM MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
15% (1%, 0.02%, 0.02%,
55
No Improvement



isothiazolone, Proclin 300 Calcium
0.002%, 10 mM)





Chloride in 10 mM MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
20% (1%, 0.02%, 0.02%,
56
No Improvement



isothiazolone, Proclin 300 Calcium
0.002%, 10 mM)





Chloride in 10 mM MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
10% (1%, 0.02%, 0.02%,
56
No Improvement



isothiazolone, Proclin 300, Magnesium
0.002%, 10 mM)





Sulfate in 10 mM MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
15% (1%, 0.02%, 0.02%,
54
No Improvement



isothiazolone, Proclin 300, Magnesium
0.002%, 10 mM)





Sulfate in 10 mM MOPS, pH 6.3





HRP Stabilizer Components
Fetal Bovine Serum, Bronidox, Methyl
20% (1%, 0.02%, 0.02%,
53
No Improvement



isothiazolone, Proclin 300, Magnesium
0.002%, 10 mM)





Sulfate in 10 mM MOPS, pH 6.3





Substrates
diphosphoglycerate
1 mM
68
No Improvement


Metal Chelators
Phytic Acid
1%
33
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
1% (325 g/L, 90 g/L,
63
No Improvement



MOPS, pH 7.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
1% (325 g/L, 90 g/L,
37
No Improvement



MOPS, pH 7.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
1% (325 g/L, 90 g/L,
38
No Improvement



MOPS, pH 7.5 + Phytic Acid
40 g/L, 1%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
1% (325 g/L, 90 g/L,
34
No Improvement



MOPS, pH 8.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
1% (325 g/L, 90 g/L,
58
No Improvement



MOPS, pH 8.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
1% (325 g/L, 90 g/L,
57
No Improvement



MOPS, pH 8.5 + Phytic Acid
40 g/L, 1%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
10% (325 g/L, 90 g/L,
65
No Improvement



MOPS, pH 7.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
10% (325 g/L, 90 g/L,
39
No Improvement



MOPS, pH 7.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
10% (325 g/L, 90 g/L,
39
No Improvement



MOPS, pH 7.5 + Phytic Acid
40 g/L, 1%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
10% (325 g/L, 90 g/L,
51
No Improvement



MOPS, pH 8.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
10% (325 g/L, 90 g/L,
70
No Improvement



MOPS, pH 8.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
10% (325 g/L, 90 g/L,
76
No Improvement



MOPS, pH 8.5 + Phytic Acid
40 g/L, 1%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
20% (325 g/L, 90 g/L,
57
No Improvement



MOPS, pH 7.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
20% (325 g/L, 90 g/L,
52
No Improvement



MOPS, pH 7.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
20% (325 g/L, 90 g/L,
49
No Improvement



MOPS, pH 7.5 + Phytic Acid
40 g/L, 1%)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
20% (325 g/L, 90 g/L,
24
No Improvement



MOPS, pH 8.5
40 g/L)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycinein 10 mM
20% (325 g/L, 90 g/L,
46
No Improvement



MOPS, pH 8.5 + Diphosphoglycerate
40 g/L, 1 mM)




Formaldehyde Releaser
Diazolidinyl Urea, EDTA, Glycine in 10 mM
20% (325 g/L, 90 g/L,
44
No Improvement



MOPS, pH 8.5 + Phytic Acid
40 g/L, 1%)




HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
86
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% (diluted 4% with
82
Improvement in % Hb



(diluted with water)
water)

Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
93
Improvement in % Hb



Calcium Chloride


Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
90
Improvement in % Hb



Magnesium Sulfate


Recovery


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
75
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
82
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% (diluted 4% with
91
Improvement in % Hb



(diluted with water)
water)

Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
90
Improvement in % Hb



Calcium Chloride (comp)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
102 
Improvement in % Hb



Calcium Chloride (spike)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
92
Improvement in % Hb



Magnesium Sulfate (comp)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
97
Improvement in % Hb



Magnesium Sulfate (spike)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
65
Improvement in % Hb



Cobalt Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
77
Improvement in % Hb



Copper Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
75
Improvement in % Hb



Iron (III) Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
90
Improvement in % Hb



Nickel Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
91
Improvement in % Hb



Zinc Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
87
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% (diluted 4% with
82
Improvement in % Hb



(diluted with water)
water)

Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
100 
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
96
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
90
Improvement in % Hb



Aluminum Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
118 
Improvement in % Hb



Lithium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
79
Improvement in % Hb



Sodium Chloride


Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
0.1 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
1 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
2 mM
72
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
4 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
6 mM
77
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
8 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
10 mM
79
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride anhydrous
20 mM
76
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
0.1 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
1 mM
70
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
2 mM
71
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
4 mM
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
6 mM
74
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
8 mM
72
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
10 mM
77
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Calcium Chloride dihydrate
20 mM
73
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Plasma
Human Plasma
1%
96
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
1%
99
No improvement. High






Background Signals


Plasma
Human Plasma
5%
97
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
5%
94
No improvement. High






Background Signals


Plasma
Human Plasma
10%
96
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
10%
98
No improvement. High






Background Signals


Plasma
Human Plasma
25%
98
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
25%
97
No improvement. High






Background Signals


Plasma
Human Plasma
50%
99
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
50%
97
No improvement. High






Background Signals


Plasma
Human Plasma
0.005%
81
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.005%
103 
No improvement. High






Background Signals


Plasma
Human Plasma
0.005%
77
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.005%
99
No improvement. High






Background Signals


Plasma
Human Plasma
0.01%
81
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.01%
106 
No improvement. High






Background Signals


Plasma
Human Plasma
0.01%
80
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.01%
101 
No improvement. High






Background Signals


Plasma
Human Plasma
0.05%
101 
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.05%
100 
No improvement. High






Background Signals


Plasma
Human Plasma
0.05%
95
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.05%
98
No improvement. High






Background Signals


Plasma
Human Plasma
0.01%
99
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.01%
99
No improvement. High






Background Signals


Plasma
Human Plasma
0.01%
99
No improvement. High






Background Signals


Plasma
Human Plasma (no sample)
0.01%
99
No improvement. High






Background Signals


Plasma
Human Albumin
0.0025 mg/mL
71
No improvement. High






Background Signals


Plasma
Human Albumin (no sample)
0.0025 mg/mL
105 
No improvement. High






Background Signals


Plasma
Human Albumin
0.025 mg/mL
90
No improvement. High






Background Signals


Plasma
Human Albumin (no sample)
0.025 mg/mL
95
No improvement. High






Background Signals


Plasma
Human Albumin
0.25 mg/mL
98
No improvement. High






Background Signals


Plasma
Human Albumin (no sample)
0.25 mg/mL
95
No improvement. High






Background Signals


Plasma
Human Albumin
2.5 mg/mL
99
No improvement. High






Background Signals


Plasma
Human Albumin (no sample)
2.5 mg/mL
98
No improvement. High






Background Signals


Plasma
Human Albumin
25 mg/mL
98
No improvement. High






Background Signals


Plasma
Human Albumin (no sample)
25 mg/mL
96
No improvement. High






Background Signals


Plasma
Glycine
0.0002 mg/mL
68
No improvement


Plasma
Glycine (no sample)
0.0002 mg/mL
88
No improvement


Plasma
Glycine
0.002 mg/mL
68
No improvement


Plasma
Glycine (no sample)
0.002 mg/mL
218 
No improvement


Plasma
Glycine
0.02 mg/mL
71
No improvement


Plasma
Glycine (no sample)
0.02 mg/mL
132 
No improvement


Plasma
Crohn's Fraction II/III
0.005 mg/mL
70
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III (no sample)
0.005 mg/mL
106 
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III
0.05 mg/mL
80
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III (no sample)
0.05 mg/mL
107 
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III
0.5 mg/mL
99
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III (no sample)
0.5 mg/mL
103 
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III
5 mg/mL
100 
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III (no sample)
5 mg/mL
97
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III
50 mg/mL
97
No improvement. High






Background Signals


Plasma
Crohn's Fraction II/III (no sample)
50 mg/mL
109 
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV
0.005 mg/mL
106 
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV (no sample)
0.005 mg/mL
93
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV
0.05 mg/mL
100 
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV (no sample)
0.05 mg/mL
88
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV
0.5 mg/mL
98
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV (no sample)
0.5 mg/mL
94
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV
5 mg/mL
96
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV (no sample)
5 mg/mL
97
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV
50 mg/mL
98
No improvement. High






Background Signals


Plasma
Crohn's Fraction IV (no sample)
50 mg/mL
98
No improvement. High






Background Signals


Plasma
HDL
0.0025 mg/mL
69
No improvement


Plasma
HDL (no sample)
0.0025 mg/mL
128 
No improvement


Plasma
HDL
0.025 mg/mL
69
No improvement


Plasma
HDL (no sample)
0.025 mg/mL
117 
No improvement


Plasma
HDL
0.25 mg/mL
70
No improvement


Plasma
HDL (no sample)
0.25 mg/mL
106 
No improvement


Plasma
LDL
0.0025 mg/mL
69
No improvement


Plasma
LDL (no sample)
0.0025 mg/mL
109 
No improvement


Plasma
LDL
0.025 mg/mL
70
No improvement


Plasma
LDL (no sample)
0.025 mg/mL
93
No improvement


Plasma
LDL
0.25 mg/mL
67
No improvement


Plasma
LDL (no sample)
0.25 mg/mL
180 
No improvement


Plasma
phosphatidylcholine
0.01 mg/mL
67
No improvement


Plasma
phosphatidylcholine (no sample)
0.01 mg/mL
80
No improvement


Plasma
phosphatidylcholine
0.1 mg/mL
68
No improvement


Plasma
phosphatidylcholine (no sample)
0.1 mg/mL
91
No improvement


Plasma
phosphatidylcholine
1 mg/mL
62
No improvement


Plasma
phosphatidylcholine (no sample)
1 mg/mL
118 
No improvement


Plasma
Bovine Serum
0.001%
71
No improvement


Plasma
Bovine Serum (no sample)
0.001%
94
No improvement


Plasma
Bovine Serum
0.01%
72
No improvement


Plasma
Bovine Serum (no sample)
0.01%
62
No improvement


Plasma
Bovine Serum
0.10%
61
No improvement


Plasma
Bovine Serum (no sample)
0.10%
71
No improvement


Plasma
Bovine Serum
1%
38
No improvement


Plasma
Bovine Serum (no sample)
1%
126 
No improvement


Plasma
Lyopholized Human Plasma
0.001%
71
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma (no sample)
0.001%
96
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma
0.01%
79
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma (no sample)
0.01%
104 
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma
0.10%
93
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma (no sample)
0.10%
99
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma
1%
95
No improvement. High






Background Signals


Plasma
Lyopholized Human Plasma (no sample)
1%
100 
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137)
0.001%
69
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137) (no sample)
0.001%
118 
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137)
0.01%
80
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137) (no sample)
0.01%
98
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137)
0.10%
95
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137) (no sample)
0.10%
101 
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137)
1%
98
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22137) (no sample)
1%
99
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355)
0.001%
78
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355) (no sample)
0.001%
81
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355)
0.01%
88
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355) (no sample)
0.01%
119 
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355)
0.10%
98
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355) (no sample)
0.10%
100 
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355)
1%
94
No improvement. High






Background Signals


Plasma
Pooled Human Plasma (22355) (no sample)
1%
99
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A)
0.001%
72
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A) (no sample)
0.001%
114 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A)
0.01%
91
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A) (no sample)
0.01%
96
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A)
0.10%
94
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A) (no sample)
0.10%
104 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A)
1%
101 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94459A) (no sample)
1%
106 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A)
0.001%
71
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A) (no sample)
0.001%
138 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A)
0.01%
87
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A) (no sample)
0.01%
137
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A)
0.10%
98
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A) (no sample)
0.10%
102 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A)
1%
96
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A) (no sample)
1%
104 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.001%
73
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.001%
101 
No improvement. High



(no sample)


Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.01%
84
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.01%
89
No improvement. High



(no sample)


Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.10%
99
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
0.10%
95
No improvement. High



(no sample)


Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
1%
101 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94460A), spun 2×
1%
105 
No improvement. High



(no sample)


Background Signals


Plasma
SD Human Plasma (23 94464A)
0.001%
77
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A) (no sample)
0.001%
91
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A)
0.01%
91
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A) (no sample)
0.01%
114 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A)
0.10%
99
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A) (no sample)
0.10%
101 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A)
1%
99
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 94464A) (no sample)
1%
103 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A)
0.001%
71
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A) (no sample)
0.001%
106 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A)
0.01%
75
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A) (no sample)
0.01%
96
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A)
0.10%
103 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A) (no sample)
0.10%
103 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A)
1%
100 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95813A) (no sample)
1%
105 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A)
0.001%
72
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A) (no sample)
0.001%
109 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A)
0.01%
77
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A) (no sample)
0.01%
136 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A)
0.10%
103 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A) (no sample)
0.10%
107 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A)
1%
101 
No improvement. High






Background Signals


Plasma
SD Human Plasma (23 95819A) (no sample)
1%
108 
No improvement. High






Background Signals


Non-covalent Hb interactions
MOPS + BSA
15% (10 mM + 1%)
76
No improvement


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
75
Calcium cation shows






improvement in % Hb






Recovery at 10 mM


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
73
Magnesium Cation shows






improvement in % Hb






Recovery around 10 mM


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
15%
76
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
87
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
89
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
15% + 4%
76
Improvement in % Hb



(diluted with water)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15%
75
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15% + 10 mM
87
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15% + 10 mM
92
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15% + 4%
77
Improvement in % Hb



(diluted with water)


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
77
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
92
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
95
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% + 4%
77
Improvement in % Hb



(diluted with water)


Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
71
No improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
79
Improvement in % Hb

















Recovery











HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
85
Improvement in % Hb



Calcium Chloride


Recovery


Sugars/Saccharides
Galaturonic Acid
1%
37
No improvement


Sugars/Saccharides
Galaturonic Acid
1% + 10 mM
41
No improvement


Sugars/Saccharides
Galaturonic Acid
0.10%
75
No improvement


Sugars/Saccharides
Galaturonic Acid
0.1% + 10 mM
72
No improvement


Sugars/Saccharides
Galaturonic Acid
0.01%
69
No improvement


Sugars/Saccharides
Galaturonic Acid
0.01% + 10 mM
73
No improvement


Sugars/Saccharides
Polygalacturonic Acid
0.20%
70
No improvement


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.2% + 10 mM
58
No improvement


Sugars/Saccharides
Polygalacturonic Acid
0.10%
70
No improvement


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.1% + 10 mM
83
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid
0.05%
68
No improvement


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.05% + 10 mM
75
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid
0.01%
69
No improvement


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.01% + 10 mM
74
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
72
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
76
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
10 mM + 10 mM
72
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
83
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
78
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM + 10 mM
82
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
15%
72
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
78
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
15% + 10 mM
76
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Buffer + CaCl2 + MgSO4
15% + 10 mM + 10 mM
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15%
71
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM
77
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM
79
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM + 10 mM
80
Improvement in % Hb



CaCl2 + MgSO4


Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
82
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10 mM
89
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
10 mM + 10 mM
81
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
10 mM + 5 mM
78
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
5 mM + 10 mM
90
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride + Magnesium Sulfate
5 mM + 5 mm
87
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
80
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
87
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
92
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM + 10 mM
87
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM + 5 mM
89
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 5 mM + 10 mM
90
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 5 mM + 5 mM
89
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
15%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM
89
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM
86
Improvement in % Hb



Magnesium Sulfate


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM + 10 mM
90
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 10 mM + 5 mM
89
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 5 mM + 10 mM
82
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
15% + 5 mM + 5 mM
87
Improvement in % Hb



CaCl2 + MgSO4


Recovery


pH Adjustments
Hb Collection Buffer, pH 6.5
NA
84
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
79
Improvement in % Hb






Recovery


HRP Stabilizer Components
HR Conjugate Stabilizer (PN 85R-102)
15% + 10 mM
89
Improvement in % Hb



Calcium Chloride


Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.01% + 10 mM
83
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.025% + 10 mM
91
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.05% + 10 mM
86
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.075% + 10 mM
79
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.1% + 10 mM
64
Improvement in % Hb






Recovery


Sugars/Saccharides
Polygalacturonic Acid + Calcium Chloride
0.125% + 10 mM
86
Improvement in % Hb






Recovery


Sugars/Saccharides
Sodium Alginate (Medium Viscosity)
0.01%
58
No Improvement


Sugars/Saccharides
Sodium Alginate (Medium Viscosity)
0.01% + 10 mM
62
No Improvement



CaCl2





Sugars/Saccharides
Sodium Alginate (Medium Viscosity)
0.05%
56
No Improvement


Sugars/Saccharides
Sodium Alginate (Medium Viscosity) +
0.5% + 10 mM
58
No Improvement



CaCl2





Sugars/Saccharides
Sodium Alginate (Medium Viscosity)
0.10%
46
No Improvement


Sugars/Saccharides
Sodium Alginate (Medium Viscosity) +
0.1% + 10 mM
45
No Improvement



CaCl2





Sugars/Saccharides
Sodium Alginate (Medium Viscosity)
0.20%
29
No Improvement


Sugars/Saccharides
Sodium Alginate (Medium Viscosity) +
0.2% + 10 mM
25
No Improvement



CaCl2





Sugars/Saccharides
Sodium Alginate (Synthetic)
0.01%
61
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic) + CaCl2
0.01% + 10 mM
62
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic)
0.05%
50
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic) + CaCl2
0.05% + 10 mM
57
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic)
0.10%
57
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic) + CaCl2
0.1% + 10 mM
52
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic)
0.20%
56
No Improvement


Sugars/Saccharides
Sodium Alginate (Synthetic) + CaCl2
0.2% + 10 mM
50
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% + 10 mM
80
Improvement in % Hb



Calcium Chloride


Recovery


Sugars/Saccharides
Sodium Alginate (Low Viscosity)
0.01%
59
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity) + CaCl2
0.01% + 10 mM
61
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity)
0.05%
53
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity) + CaCl2
0.05% + 10 mM
59
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity)
0.10%
54
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity) + CaCl2
0.1% + 10 mM
64
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity)
0.20%
48
No Improvement


Sugars/Saccharides
Sodium Alginate (Low Viscosity) + CaCl2
0.2% + 10 mM
54
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
79
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
81
Improvement in % Hb



Calcium Chloride


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
100%
78
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
100% + 10 mM
83
Improvement in % Hb



CaCl2


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
100% + 10 mM
78
Improvement in % Hb



MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
100% + 10 mM + 10 mM
82
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537)
100%
72
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
100% + 10 mM
78
Improvement in % Hb



CaCl2


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
100% + 10 mM
76
Improvement in % Hb



MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN ab171537) +
100% + 10 mM + 10 mM
78
Improvement in % Hb



CaCl2 + MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
100%
71
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
100% + 10 mM
77
Improvement in % Hb



CaCl2


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
100% + 10 mM
79
Improvement in % Hb



MgSO4


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02) +
100% + 10 mM + 10 mM
80
Improvement in % Hb



CaCl2 + MgSO4


Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride, pH 6.5
10 mM
72
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102),
15%
87
Improvement in % Hb



pH 6.5


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102) +
15% + 10 mM
86
Improvement in % Hb



CaCl2, pH 6.5


Recovery


HRP Stabilizer Components
BSA
10%
66
No Improvement


HRP Stabilizer Components
BSA, pH 6.5
10%
69
No Improvement


HRP Stabilizer Components
BSA + HRP Conjugate Stabilizer (PN 85R-
10% + 15%
80
Improvement in % Hb



102), pH 6.5


Recovery


HRP Stabilizer Components
BSA
5%
67
No Improvement


HRP Stabilizer Components
BSA, pH 6.5
5%
67
No Improvement


HRP Stabilizer Components
BSA + HRP Conjugate Stabilizer (PN 85R-
5% + 15%
74
Improvement in % Hb



102), pH 6.5


Recovery


HRP Stabilizer Components
BSA
1%
56
No Improvement


HRP Stabilizer Components
BSA, pH 6.5
1%
60
No Improvement


HRP Stabilizer Components
BSA + HRP Conjugate Stabilizer (PN 85R-
1% + 15%
75
Improvement in % Hb



102), pH 6.5


Recovery


HRP Stabilizer Components
BSA
0.50%
50
No Improvement


HRP Stabilizer Components
BSA, pH 6.5
0.50%
56
No Improvement


HRP Stabilizer Components
BSA + HRP Conjugate Stabilizer (PN 85R-
0.5% + 15%
80
Improvement in % Hb



102), pH 6.5


Recovery


HRP Stabilizer Components
BSA
0%
47
No Improvement


HRP Stabilizer Components
BSA, pH 6.5
0%
51
No Improvement


HRP Stabilizer Components
BSA + HRP Conjugate Stabilizer (PN 85R-
0% + 15%
83
Improvement in % Hb



102), pH 6.5


Recovery


Protoporphyrin
Protoporphyrin IX
1 uM
68
No Improvement


Protoporphyrin
Protoporphyrin IX
10 uM
70
No Improvement


Protoporphyrin
Protoporphyrin IX
100 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
96
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
10 uM
72
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
100 uM
73
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
1 uM + 1 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
10 uM + 10 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
100 uM + 100 uM
83
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + Iron (II) Chloride
1 uM + 1 uM
71
No Improvement


Protoporphyrin
Protoporphyrin IX + Iron (II) Chloride
10 uM + 10 uM
65
No Improvement


Protoporphyrin
Protoporphyrin IX + Iron (II) Chloride
100 uM + 100 uM
71
No Improvement


Protoporphyrin
Protoporphyrin IX + Iron (III) Chloride
1 uM + 1 uM
65
No Improvement


Protoporphyrin
Protoporphyrin IX + Iron (III) Chloride
10 uM + 10 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX + Iron (III) Chloride
100 uM + 100 uM
60
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02)
0.15
70
No Improvement


Protoporphyrin
Protoporphyrin IX
0.001 uM
54
No Improvement


Protoporphyrin
Protoporphyrin IX
0.01 uM
50
No Improvement


Protoporphyrin
Protoporphyrin IX
0.1 uM
50
No Improvement


Protoporphyrin
Protoporphyrin IX
1 uM
74
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.001 uM
51
No Improvement


Protoporphyrin
Protoporphyrin IX Zinc
0.01 uM
73
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
88
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
66
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
0.001 uM + 0.001 uM
59
No Improvement


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
0.01 uM + 0.01 uM
51
No Improvement


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
0.1 uM + 0.1 uM
57
No Improvement


Protoporphyrin
Protoporphyrin IX + Zinc Chloride
1 uM + 1 uM
53
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
66
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102),
15%
93
Improvement in % Hb



pH 6.5


Recovery


Protoporphyrin
Protoporphyrin IX
0.1 uM
68
No Improvement


Protoporphyrin
Protoporphyrin IX
1 uM
69
No Improvement


Protoporphyrin
Protoporphyrin IX
10 uM
63
No Improvement


Protoporphyrin
Protoporphyrin IX Copper
0.1 uM
70
No Improvement


Protoporphyrin
Protoporphyrin IX Copper
1 uM
67
No Improvement


Protoporphyrin
Protoporphyrin IX Copper
10 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX Magnesium
0.1 uM
65
No Improvement


Protoporphyrin
Protoporphyrin IX Magnesium
1 uM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Magnesium
10 uM
73
No Improvement


Protoporphyrin
Protoporphyrin IX Tin
0.1 uM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Tin
1 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX Tin
10 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
83
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
10 uM
90
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102),
15%
89
Improvement in % Hb



pH 6.5


Recovery


Protoporphyrin
Hemin, pH 6.5
0.0003%
101
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
1.5 mM + 1.5 mM
80
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
22.5 mM + 22.5 mM
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
3 mM + 1.5 mM
81
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
45 mM + 22.5 mM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
4.5 mM + 1.5 mM
85
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA
67.5 mM + 22.5 mM
88
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Tin + BSA
1.5 mM + 1.5 mM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Tin + BSA
22.5 mM + 22.5 mM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Tin + BSA
3 mM + 1.5 mM
68
No Improvement


Protoporphyrin
Protoporphyrin IX Tin + BSA
45 mM + 22.5 mM
70
No Improvement


Protoporphyrin
Protoporphyrin IX Tin + BSA
4.5 mM + 1.5 mM
71
No Improvement


Protoporphyrin
Protoporphyrin IX Tin + BSA
67.5 mM + 22.5 mM
70
No Improvement


Protoporphyrin
Protoporphyrin IX Zinc + BSA
1.5 mM + 1.5 mM
88
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc + BSA
22.5 mM + 22.5 mM
86
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc + BSA
3 mM + 1.5 mM
90
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc + BSA
45 mM + 22.5 mM
87
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc + BSA
4.5 mM + 1.5 mM
82
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc + BSA
67.5 mM + 22.5 mM
78
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX + BSA
1.5 mM + 1.5 mM
66
No Improvement


Protoporphyrin
Protoporphyrin IX + BSA
22.5 mM + 22.5 mM
63
No Improvement


Protoporphyrin
Protoporphyrin IX + BSA
3 mM + 1.5 mM
64
No Improvement


Protoporphyrin
Protoporphyrin IX + BSA
45 mM + 22.5 mM
69
No Improvement


Protoporphyrin
Protoporphyrin IX + BSA
4.5 mM + 1.5 mM
66
No Improvement


Protoporphyrin
Protoporphyrin IX + BSA
67.5 mM + 22.5 mM
65
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02),
15%
95
Improvement in % Hb



pH 6.5


Recovery


Protoporphyrin
Protoporphyrin IX
0.1 uM
67
No Improvement


Protoporphyrin
Protoporphyrin IX
1 uM
74
No Improvement


Protoporphyrin
Protoporphyrin IX
10 uM
64
No Improvement


Protoporphyrin
Protoporphyrin IX Cobalt
0.1 uM
72
No Improvement


Protoporphyrin
Protoporphyrin IX Cobalt
1 uM
78
No Improvement


Protoporphyrin
Protoporphyrin IX Cobalt
10 uM
116
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
0.1 uM
71
No Improvement


Protoporphyrin
Protoporphyrin IX Chromium
1 uM
82
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
10 uM
117
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Mangansese
0.1 uM
74
No Improvement


Protoporphyrin
Protoporphyrin IX Mangansese
1 uM
72
No Improvement


Protoporphyrin
Protoporphyrin IX Mangansese
10 uM
68
No Improvement


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
95
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
10 uM
93
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02),
15%
91
No Improvement



pH 6.5





Protoporphyrin
Protoporphyrin IX Cobalt
1 uM
75
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt
2.5 uM
70
No Improvement


Protoporphyrin
Protoporphyrin IX Cobalt
5 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
1 uM
83
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
2.5 uM
96
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
5 uM
93
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Magnesium
10 uM
61
No Improvement


Protoporphyrin
Protoporphyrin IX Magnesium
25 uM
66
No Improvement


Protoporphyrin
Protoporphyrin IX Magnesium
50 uM
76
No Improvement


Protoporphyrin
Protoporphyrin IX Mangansese
0.025 uM
54
No Improvement


Protoporphyrin
Protoporphyrin IX Mangansese
0.05 uM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Mangansese
0.1 uM
63
No Improvement


Protoporphyrin
Hemin, pH 6.5
0.00%
82
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102),
15%
91
Improvement in % Hb



pH 6.5


Recovery


Protoporphyrin
Protoporphyrin IX Cobalt
5 uM
103 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
2.5 uM
109 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
82
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
0.1 uM
83
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
85
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
1 uM
95
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
84
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
0.1 uM
82
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
1 uM
103 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
79
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
0.1 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
81
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
1 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
0.1 uM
85
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
0.1 uM
77
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc
1 uM
86
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Zinc, pH 6.5
1 uM
82
Improvement in % Hb






Recovery


Polyvalent Ions/Metal Salts
Calcium Chloride
10 mM
71
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
79
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15% + 10 mM
85
No Improvement



CaCl2





Saccharides/Sugars
Galaturonic Acid
1%
37
No Improvement


Saccharides/Sugars
Galaturonic Acid + CaCl2
1% + 10 mM
41
No Improvement


Saccharides/Sugars
Galaturonic Acid
0.10%
75
No Improvement


Saccharides/Sugars
Galaturonic Acid + CaCl2
0.1% + 10 mM
72
No Improvement


Saccharides/Sugars
Galaturonic Acid
0.01%
69
No Improvement


Saccharides/Sugars
Galaturonic Acid + CaCl2
0.01% + 10 mM
73
No Improvement


Saccharides/Sugars
Polygalacturonic Acid
0.20%
70
No Improvement


Saccharides/Sugars
Polygalacturonic Acid + CaCl2
0.2% + 10 mM
58
No Improvement


Saccharides/Sugars
Polygalacturonic Acid
0.10%
70
No Improvement


Saccharides/Sugars
Polygalacturonic Acid + CaCl2
0.1% + 10 mM
83
Improvement in % Hb






Recovery


Saccharides/Sugars
Polygalacturonic Acid
0.05%
68
No Improvement


Saccharides/Sugars
Polygalacturonic Acid + CaCl2
0.05% + 10 mM
75
No Improvement


Saccharides/Sugars
Polygalacturonic Acid
0.01%
69
No Improvement


Saccharides/Sugars
Polygalacturonic Acid + CaCl2
0.01% + 10 mM
74
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
84
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (DMSO)
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.0003%
84
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (NaHCO3)
0.0003%
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (DMSO)
0.15% + 0.0003%
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (H2O:NaOH)
0.15% + 0.0003%
87
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (NaHCO3)
0.15% + 0.0003%
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (DMSO)
0.001% + 0.0001%
85
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (DMSO)
0.01% + 0.001%
93
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (DMSO)
0.1% + 0.01%
85
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (H2O:NaOH)
0.001% + 0.0001%
85
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (H2O:NaOH)
0.01% + 0.001%
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (H2O:NaOH)
0.1% + 0.01%
83
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (NaHCO3)
0.001% + 0.0001%
85
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (NaHCO3)
0.01% + 0.001%
91
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin (NaHCO3)
0.1% + 0.01%
72
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102),
15%
93
Improvement in % Hb



pH 6.5


Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
83
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.0003%
86
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00005%
82
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00009%
87
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00018%
86
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00037%
85
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00075%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.00015%
87
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.003%
90
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00005%
90
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00009%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00018%
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00037%
95
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00075%
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.00015%
94
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH), pH 6.5
0.003%
89
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
95
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.0003%
90
Improvement in % Hb






Recovery


Alternate Hb CB Formulation
Hb CB 10% BSA
NA
75
NA


Alternate Hb CB Formulation
Hb CB 5% BSA
NA
73
NA


Alternate Hb CB Formulation
Hb CB 1% BSA
NA
66
NA


Alternate Hb CB Formulation
Hb CB 0% BSA
NA
53
NA


Protoporphyrin
Hemin + Hb CB 10% BSA
0.00003%
75
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 5% BSA
0.00003%
80
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 1% BSA
0.00003%
74
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 0% BSA
0.00003%
65
No Improvement


Protoporphyrin
Hemin + Hb CB 10% BSA
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 5% BSA
0.0003%
90
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 1% BSA
0.0003%
86
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 0% BSA
0.0003%
71
No Improvement


Protoporphyrin
Hemin + Hb CB 10% BSA
0.003%
84
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 5% BSA
0.003%
85
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Hb CB 1% BSA
0.003%
68
No Improvement


Protoporphyrin
Hemin + Hb CB 0% BSA
0.003%
62
No Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.0003%
87
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + CaCl2
0.0003% + 10 mM
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + MgSO4
0.0003% + 10 mM
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl2
0.0003% + 4.8 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl2
0.0003% + 9.6 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl2
0.0003% + 2.4 uM
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl3
0.0003% + 4.8 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl3
0.0003% + 9.6 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + FeCl3
0.0003% + 2.4 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin
1% + 0.0003%
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + CaCl2
1% + 0.0003% + 10 mM
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + MgSO4
1% + 0.0003% + 10 mM
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl2
1% + 0.0003% + 4.8 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl2
1% + 0.0003% + 9.6 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl2
1% + 0.0003% + 2.4 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl3
1% + 0.0003% + 4.8 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl3
1% + 0.0003% + 9.6 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
BSA + Hemin + FeCl3
1% + 0.0003% + 2.4 uM
88
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN 85R-102)
15%
94
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.4
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.5
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.6
0.0003%
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.7
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.8
0.0003%
89
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 6.9
0.0003%
85
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.0
0.0003%
87
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.1
0.0003%
88
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.2
0.0003%
83
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.3
0.0003%
81
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.4
0.0003%
81
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, pH 7.5
0.0003%
79
Improvement in % Hb






Recovery


HRP Stabilizer Components
HRP Conjugate Stabilizer (PN SZ02),
15%
93
Improvement in % Hb



pH 6.5


Recovery


Protoporphyrin
Hemin (H2O:NaOH)
0.0003%
95
Improvement in % Hb






Recovery


Alternate Hb CB Formulation
BSA
22.5 uM
80
Improvement in % Hb






Recovery


Protoporphyrin
Hemin
4.5 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Sodium Azide
4.5 uM + 0.0072%
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Sodium Azide
4.5 uM + 0.014%
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + Sodium Azide
4.5 uM + 0.029%
92
Improvement in % Hb






Recovery


Alternate Hb CB Formulation
Sodium Azide
0.0072%
72
No Improvement


Alternate Hb CB Formulation
Sodium Azide
0.014%
72
No Improvement


Alternate Hb CB Formulation
Sodium Azide
0.029%
73
No Improvement


Protoporphyrin
Hemin + BSA
4.5 uM + 22.5 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA + Sodium Azide
4.5 uM + 22.5 uM + 0.0072%
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA + Sodium Azide
4.5 uM + 22.5 uM + 0.014%
89
Improvement in % Hb






Recovery


Protoporphyrin
Hemin + BSA + Sodium Azide
4.5 uM + 22.5 uM + 0.029%
93
Improvement in % Hb






Recovery


Protoporphyrin
Hemin
4.8 uM
81
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
2.5 uM
105 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 1)
1.25 uM
76
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 1)
2.5 uM
85
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 1)
5 uM
103 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 1)
6.5 uM
102 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 2)
1.25 uM
75
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 2)
2.5 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 2)
5 uM
97
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 2)
6.5 uM
108 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 3)
1.25 uM
71
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 3)
2.5 uM
83
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 3)
5 uM
103 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt (Vendor 3)
6.5 uM
112 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Gallium
1.25 uM
68
No Improvement


Protoporphyrin
Protoporphyrin IX Gallium
2.5 uM
69
No Improvement


Protoporphyrin
Protoporphyrin IX Gallium
5 uM
71
No Improvement


Protoporphyrin
Protoporphyrin IX Gallium
6.5 uM
70
No Improvement


Protoporphyrin
Protoporphyrin IX Cobalt
5 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 1)
1.25 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 1)
2.5 uM
78
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 1)
5 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 1)
6.5 uM
104 
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 2)
1.25 uM
72
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 2)
2.5 uM
71
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 2)
5 uM
82
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium (Vendor 2)
6.5 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Cobalt
5 uM
84
Improvement in % Hb






Recovery


Protoporphyrin
Protoporphyrin IX Chromium
2.5 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Bovine
2.4 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Bovine
4.8 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Bovine
9.6 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
2.4 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
3.6 uM
84
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
4.8 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
6 uM
89
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
7.2 uM
87
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
8.4 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hematin
9.6 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 1)
2.4 uM
91
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 1)
4.8 uM
104 
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 1)
9.6 uM
92
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 2)
2.4 uM
86
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 2)
4.8 uM
90
Improvement in % Hb






Recovery


Protoporphyrin
Hemin, Porcine (Vendor 2)
9.6 uM
92
Improvement in % Hb






Recovery


Non covalent Hb interactions
Polyvinylpyrrolidone (10,000 Da)
1%
68
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (10,000 Da)
0.50%
65
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (10,000 Da)
0.10%
70
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (10,000 Da)
0.05%
67
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (10,000 Da) + CaCl2
0.1% + 10 mM
77
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (40,000 Da)
1%
68
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (40,000 Da)
0.50%
67
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (40,000 Da)
0.10%
65
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (40,000 Da)
0.05%
68
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (40,000 Da) + CaCl2
0.1% + 10 mM
75
No Improvement


Non covalent Hb interactions
Propylene Glycol
1%
67
No Improvement


Non covalent Hb interactions
Propylene Glycol
0.50%
65
No Improvement


Non covalent Hb interactions
Propylene Glycol
0.10%
69
No Improvement


Protoporphyrin
Bilirubin
0.00003%
70
No Improvement


Protoporphyrin
Bilirubin
0.00030%
68
No Improvement


Protoporphyrin
Bilirubin
0.00300%
68
No Improvement


Protoporphyrin
Bilirubin
0.03000%
66
No Improvement


Protoporphyrin
Biliverdin
0.00003%
68
No Improvement


Protoporphyrin
Biliverdin
0.00030%
66
No Improvement


Protoporphyrin
Biliverdin
0.00300%
67
No Improvement


Protoporphyrin
Biliverdin
0.03000%
60
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (350,000 Da)
0.10%
71
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (350,000 Da)
0.50%
69
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (350,000 Da)
1%
77
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (350,000 Da)
2%
54
No Improvement


Non covalent Hb interactions
Polyvinylpyrrolidone (350,000 Da) + CaCl2
0.5% + 10 mM
76
No Improvement




















APPENDIX C







Additive
% Hb



Compound Classification
Additive
Concentration
Recovery
Results




















Hb Collection Buffer - No
NA
70
Current Buffer



additive


Formulation



Polymedco Buffer - No
NA
80




Additive





Preservatives with thiol
Sodium Thiosulfate
0.02%
52
No Improvement


groups






Preservatives with thiol
Sodium Thiosulfate
0.01%
59
No Improvement


groups






Preservatives with thiol
Sodium Thiosulfate
0.005% 
62
No Improvement


groups






Preservatives with thiol
Sodium Thiosulfate
0.0025% 
65
No Improvement


groups






Redox/antioxidants
(±)-α-Lipoic acid
0.0126% 
64
No Improvement


Redox/antioxidants
(±)-α-Lipoic acid
0.0063% 
69
No Improvement


Redox/antioxidants
(±)-α-Lipoic acid
0.0032% 
69
No Improvement


Redox/antioxidants
(±)-α-Lipoic acid
0.0016% 
70
No Improvement












Metal Chelators
Ethylenediamine-N,N′-
1
mM
68
No Improvement



disuccinic acid (EDDS)






Metal Chelators
Ethylenediamine-N,N′-
10
mM
58
No Improvement



disuccinic acid (EDDS)






Crosslinkers
Poly (acrylic acid)
0.1
mM
66
No Improvement


Crosslinkers
Poly (acrylic acid)
1
mM
56
No Improvement


Similar to Drabkin's Reagent
Imidazole
0.25
mM
68
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Imidazole
0.5
mM
63
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Hydroxylamine
0.25
mM
66
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Hydroxylamine
0.5
mM
66
No Improvement


(cyanide)







Non-covalent Hb
Dextran Sulphate
0.25
mM
69
No Improvement


interactions







Non-covalent Hb
Dextran Sulphate
0.5
mM
65
No Improvement


interactions







Redox/antioxidants
mPEG2K-Lipoic Acid
0.1
mM
67
No Improvement


Redox/antioxidants
mPEG2K-Lipoic Acid
0.5
mM
64
No Improvement


Redox/antioxidants
Urea
50
mM
60
No Improvement


Redox/antioxidants
Urea
100
mM
63
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
1
mM
65
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
10
mM
62
No Improvement


Metal Chelators
N,N-is-(Carboxymethyl)-L-
1
mM
65
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Metal Chelators
N,N-is-(Carboxymethyl)-L-
10
mM
40
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Preservatives with thiol
Cysteine
1
mM
46
No Improvement


groups







Preservatives with thiol
Cysteine
10
mM
36
No Improvement


groups







Preservatives with thiol
Glutathione
1
mM
62
No Improvement


groups







Preservatives with thiol
Glutathione
3
mM
62
No Improvement


groups







Preservatives with thiol
Dithiothreitol
0.1
mM
63
No Improvement


groups







Preservatives with thiol
Dithiothreitol
1
mM
59
No Improvement


groups







Preservatives with thiol
TCEP
0.1
mM
64
No Improvement


groups







Preservatives with thiol
TCEP
1
mM
70
No Improvement


groups







Sugars/Saccharides
Raffinose
5
mM
69
No Improvement


Sugars/Saccharides
Raffinose
24
mM
65
No improvement


Redox/antioxidants
Ascorbic Acid
10
mM
38
No Improvement


Redox/antioxidants
Ascorbic Acid
25
mM
22
No Improvement


Metal Chelators
Deferoxamine Mesylate Salt
1
mM
67
No Improvement


Metal Chelators
Deferoxamine Mesylate Salt
5
mM
58
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
1
mM
65
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
10
mM
62
No Improvement


Substrates
Diphosphoglycerate (DPG)
1
mM
68
No Improvement


Crosslinkers
Poly (acrylic acid)
0.01
mM
65
No Improvement


Crosslinkers
Poly (acrylic acid)
0.001
mM
68
No Improvement


Preservatives with thiol
Cysteine
0.01
mM
68
No Improvement


groups







Preservatives with thiol
Cysteine
0.1
mM
61
No Improvement


groups







Non-covalent Hb
Dextran Sulphate
0.0025
mM
69
No Improvement


interactions







Non-covalent Hb
Dextran Sulphate
0.025
mM
66
No Improvement


interactions







Substrates
Diphosphoglycerate (DPG)
0.0005
mM
70
No Improvement


Substrates
Diphosphoglycerate (DPG)
0.002
mM
66
No Improvement


Metal Chelators
N,N-is-(Carboxymethyl)-L-
0.01
mM
70
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Metal Chelators
N,N-is-(Carboxymethyl)-L-
0.1
mM
67
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Preservatives with thiol
TCEP
0.005
mM
59
No Improvement


groups







Preservatives with thiol
TCEP
0.01
mM
28
No Improvement


groups







Metal Chelators
EDTA: Disodium Salt
5
mM
66
No Improvement


Metal Chelators
EDTA: Disodium Salt
10
mM
63
No Improvement


Metal Chelators
EDTA: Disodium Salt
30
mM
59
No Improvement


Non-covalent Hb
Heparin
2.5
uM
68
No Improvement


interactions







Non-covalent Hb
Heparin
25
uM
65
No Improvement


interactions







Non-covalent Hb
Heparin
250
uM
66
No Improvement


interactions







Sulfonic Acids
Methanedisulfonic acid
0.114
mM
66
No Improvement



dipotassium salt






Sulfonic Acids
Methanedisulfonic acid
1.14
mM
67
No Improvement



dipotassium salt






Sulfonic Acids
Methanedisulfonic acid
10.69
mM
66
No Improvement



dipotassium salt






Non-covalent Hb
PEG20000
0.024
mM
66
No Improvement


interactions







Non-covalent Hb
PEG20000
0.24
mM
68
No Improvement


interactions







Non-covalent Hb
PEG20000
24
mM
62
No Improvement


interactions







Sulfonic Acids
Propanedisulfonic acid
0.4
mM
64
No Improvement



disodium salt






Sulfonic Acids
Propanedisulfonic acid
4
mM
66
No Improvement



disodium salt






Sulfonic Acids
Propanedisulfonic acid
40
mM
66
No Improvement



disodium salt






Flavoniods
Quercetin
0.6
uM
64
No Improvement


Flavoniods
Quercetin
6
uM
67
No Improvement


Flavoniods
Quercetin
60
uM
59
No Improvement


Redox/antioxidants
Uric Acid
0.6
uM
67
No Improvement


Redox/antioxidants
Uric Acid
6
uM
65
No Improvement


Redox/antioxidants
Uric Acid
60
uM
65
No Improvement


Redox/antioxidants
Ascorbic Acid
10
uM
68
No Improvement


Redox/antioxidants
Ascorbic Acid
100
uM
65
No Improvement


Osmolytes
Betaine
50
uM
56
No Improvement


Osmolytes
Betaine
500
uM
63
No Improvement


Metal Chelators
Deferoxamine Mesylate Salt
10
uM
63
No Improvement


Metal Chelators
Deferoxamine Mesylate Salt
100
uM
59
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
10
uM
68
No Improvement


Formaldehyde Releaser
Diazolidinyl Urea
100
uM
62
No Improvement


Preservatives with thiol
Dithiothreitol
1
uM
65
No Improvement


groups







Preservatives with thiol
Dithiothreitol
10
uM
61
No Improvement


groups







Metal Chelators
Ethylenediamine-N,N′-
10
uM
66
No Improvement



disuccinic acid (EDDS)






Metal Chelators
Ethylenediamine-N,N′-
100
uM
64
No Improvement



disuccinic acid (EDDS)






Preservatives with thiol
Glutathione
10
uM
65
No Improvement


groups







Preservatives with thiol
Glutathione
100
uM
56
No Improvement


groups







Similar to Drabkin's Reagent
Hydroxylamine
2.5
uM
63
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Hydroxylamine
25
uM
63
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Imidazole
2.5
uM
70
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Imidazole
25
uM
66
No Improvement


(cyanide)







Formaldehyde Releaser
Imidazolidinyl Urea
10
uM
68
No Improvement


Formaldehyde Releaser
Imidazolidinyl Urea
100
uM
65
No Improvement


Redox/antioxidants
mPEG2K-Lipoic Acid
10
uM
66
No Improvement


Redox/antioxidants
mPEG2K-Lipoic Acid
100
uM
65
No Improvement


Sugars/Saccharides
Raffinose
5
uM
66
No Improvement


Sugars/Saccharides
Raffinose
50
uM
64
No Improvement


Osmolytes
Trimethylamine N-oxide
500
uM
64
No Improvement



(TMANO)






Osmolytes
Trimethylamine N-oxide
5
mM
63
No Improvement



(TMANO)






Redox/antioxidants
Urea
500
uM
66
No Improvement


Redox/antioxidants
Urea
5
mM
62
No Improvement


Metal Chelators
N,N-is-(Carboxymethyl)-L-
10
mM
42
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Metal Chelators
Iminodisuccinic acid
10
mM
59
No Improvement


Preservatives with thiol
Cysteine
0.1
uM
65
No Improvement


groups







Preservatives with thiol
Cysteine
1
uM
67
No Improvement


groups







Preservatives with thiol
Cysteine
5
uM
65
No Improvement


groups







Non-covalent Hb
Dextran Sulphate
0.2
uM
69
No Improvement


interactions







Non-covalent Hb
Dextran Sulphate
1
uM
65
No Improvement


interactions







Non-covalent Hb
Dextran Sulphate
2
uM
67
No Improvement


interactions







Substrates
Diphosphoglycerate (DPG)
0.5
uM
67
No Improvement


Substrates
Diphosphoglycerate (DPG)
5
uM
68
No Improvement


Substrates
Diphosphoglycerate (DPG)
50
uM
66
No Improvement


Metal Chelators
N,N-is-(Carboxymethyl)-L-
0.1
uM
67
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Metal Chelators
N,N-is-(Carboxymethyl)-L-
1
uM
65
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Metal Chelators
N,N-is-(Carboxymethyl)-L-
5
uM
68
No Improvement



glutamic acid tetrasodiumn







salt (GLDA)






Non-covalent Hb
PEG2000
0.033
uM
68
No Improvement


interactions







Non-covalent Hb
PEG2000
0.33
uM
66
No Improvement


interactions







Non-covalent Hb
PEG2000
3.3
uM
66
No Improvement


interactions







Redox/antioxidants
mPEG2K-Lipoic Acid
0.1
uM
67
No Improvement


Redox/antioxidants
mPEG2K-Lipoic Acid
1
uM
65
No Improvement


Redox/antioxidants
mPEG2K-Lipoic Acid
5
uM
66
No Improvement


Preservatives with thiol
TCEP
250
uM
62
No Improvement


groups







Preservatives with thiol
TCEP
500
uM
66
No Improvement


groups







Preservatives with thiol
TCEP
750
uM
68
No Improvement


groups







Redox/antioxidants
Ascorbic Acid
0.2
uM
69
No Improvement


Redox/antioxidants
Ascorbic Acid
l
uM
68
No Improvement


Redox/antioxidants
Ascorbic Acid
5
uM
64
No Improvement











Formaldehyde Releaser
Diazolidinyl Urea +
10 uM and 10
65
No Improvement



Imidazolidinyl Urea
uM




Formaldehyde Releaser
Diazolidinyl Urea +
100 uM and
56
No Improvement



Imidazolidinyl Urea
100 uM




Formaldehyde Releaser
Diazolidinyl Urea +
1 mM and 1
61
No Improvement



Imidazolidinyl Urea
mM














Sugars/Saccharides
Glucose
72
uM
67
No Improvement


Sugars/Saccharides
Glucose
720
uM
69
No Improvement


Sugars/Saccharides
Glucose
7.2
mM
67
No Improvement


Crosslinkers
Magnesium Gluconate
1.2
uM
68
No Improvement


Crosslinkers
Magnesium Gluconate
12
uM
63
No Improvement


Crosslinkers
Magnesium Gluconate
120
uM
71
No Improvement


Enzyme inhibitors
Sodium Fluoride
8.5
uM
68
No Improvement


Enzyme Inhibitors
Sodium Fluoride
85
uM
67
No Improvement


Enzyme Inhibitors
Sodium Fluoride
850
uM
70
No Improvement











Enzyme Inhibitors
Sodium Fluoride and
950 uM and
65
No Improvement



Glucose
7.2 mM




Enzyme Inhibitors
Sodium Fluoride and
950 uM and
67
No Improvement



Glucose
72 uM




Enzyme Inhibitors
Sodium Fluoride and
950 uM and
65
No Improvement



Glucose
720 uM














Redox/antioxidants
Urea
2.5
uM
70
No Improvement


Redox/antioxidants
Urea
25
uM
69
No Improvement


Redox/antioxidants
Urea
250
uM
70
No Improvement











Formaldehyde +
Ammonium Acetate +
10 mM +
66
No Improvement


Ammonium Salt Complexes
Formaldehyde
0.02%




Formaldehyde +
Ammonium Acetate +
100 mM +
58
No Improvement


Ammonium Salt Complexes
Formaldehyde
0.1%




Formaldehyde +
Ammonium Acetate +
767 mM + 1%
37
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Chloride +
10 mM + 0.02%
63
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Chloride +
50 mM + 0.1%
56
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Chloride +
95 mM + 1%
17
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Oxalate +
0.1 mM + 0.2%
65
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Oxalate +
1 mM + 0.1%
55
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Oxalate +
4.8 mM + 1%
19
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Sulfate +
1 mM + 0.02%
65
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Sulfate +
10 mM + 0.1%
58
No Improvement


Ammonium Salt Complexes
Formaldehyde





Formaldehyde +
Ammonium Sulfate +
243 mM + 1%
11
No Improvement


Ammonium Salt Complexes
Formaldehyde















Metal Chelators
ATMP
0.02
mM
65
No Improvement


Metal Chelators
ATMP
0.2
mM
67
No Improvement


Metal Chelators
ATMP
2
mM
42
No Improvement


Crosslinkers
Cysteamine
0.016
mM
66
No Improvement


Crosslinkers
Cysteamine
0.16
mM
62
No Improvement


Crosslinkers
Cysteamine
1.6
mM
45
No Improvement


Formaldehyde +
Hexamethylenetetramine
14
mM
63
No Improvement


Ammonium Salt Complexes







Formaldehyde +
Hexamethylenetetramine
140
mM
55
No Improvement


Ammonium Salt Complexes







Formaldehyde +
Hexamethylenetetramine
500
mM
52
No Improvement


Ammonium Salt Complexes







Osmolytes
Betaine
50
mM
55
No Improvement


Osmolytes
Betaine
96
mM
49
No Improvement


Osmolytes
Trimethylamine N-oxide
50
mM
65
No Improvement



(TMANO)






Osmolytes
Trimethylamine N-oxide
87
mM
65
No Improvement



(TMANO)






Metal Chelators
Trisodium N-(1-
0.1
mM
69
No Improvement



Carboxylatoethyl)iminodiacetate







Hydrate (MGDA)






Metal Chelators
Trisodium N-(1-
1
mM
65
No Improvement



Carboxylatoethyl)iminodiacetate







Hydrate (MGDA)






Metal Chelators
Trisodium N-(1-
10
mM
49
No Improvement



Carboxylatoethyl)iminodiacetate







Hydrate (MGDA)






Flavoniods
7,8 dihydroxyflavone
0.01
mM
60
No Improvement



hydrate






Flavoniods
7,8 dihydroxyflavone
0.1
mM
59
No improvement



hydrate






Flavoniods
7,8 dihydroxyflavone
1
mM
36
No Improvement



hydrate






Crosslinkers
Bis(3,5-
5
uM
65
No Improvement



dibromosalilcyl)fumarate






Crosslinkers
Bis(3,5-
10
uM
59
No Improvement



dibromosalilcyl)fumarate






Crosslinkers
Bis(3,5-
100
uM
43
No Improvement



dibromosalilcyl)fumarate






Flavoniods
Daidzein
0.01
mM
66
No Improvement


Flavoniods
Daidzein
0.1
mM
62
No Improvement


Flavoniods
Daidzein
1
mM
42
No Improvement


Substrates
Arginine
0.058
mM
66
No Improvement


Substrates
Arginine
0.58
mM
68
No Improvement


Substrates
Arginine
5.8
mM
59
No Improvement


Flavoniods
Chrysin
0.0254
mM
67
No Improvement


Flavoniods
Chrysin
0.254
mM
56
No Improvement


Flavoniods
Chrysin
2.54
mM
44
No Improvement


Crosslinkers
Dimethyl 3,3′-
0.0032
mM
67
No Improvement



dithioproprionimidate







dihydrochloride






Crosslinkers
Dimethyl 3,3′-
0.032
mM
63
No Improvement



dithioproprionimidate







dihydrochloride






Crosslinkers
Dimethyl 3,3′-
0.32
mM
52
No Improvement



dithioproprionimidate







dihydrochloride






Substrates
Lysine
0.0687
mM
67
No Improvement


Substrates
Lysine
0.687
mM
67
No Improvement


Substrates
Lysine
6.87
mM
59
No Improvement


Substrates
Sodium Bicarbonate
0.0895
mM
71
No Improvement


Substrates
Sodium Bicarbonate
0.895
mM
66
No Improvement


Substrates
Sodium Bicarbonate
8.95
mM
61
No Improvement


Sugars/Saccharides
Trehalose
0.0174
mM
65
No Improvement


Sugars/Saccharides
Trehalose
0.174
mM
68
No Improvement


Sugars/Saccharides
Trehalose
1.74
mM
66
No Improvement


Ionic Liquid
1-Allyl-3-methylimidazolium
0.01
mM
69
No Improvement



Chloride






Ionic Liquid
1-Allyl-3-methylimidazolium
0.1
mM
64
No Improvement



Chloride






Ionic Liquid
1-Allyl-3-methylimidazolium
1
mM
70
No Improvement



Chloride















Ionic Liquid
1-Allyl-3-methylimidazolium
0.01 mM +
68
No Improvement



Chloride + NaBr
0.01 mM




Ionic Liquid
1-Allyl-3-methylimidazolium
0.1 mM + 0.1
66
No Improvement



Chloride + NaBr
mM




Ionic Liquid
1-Allyl-3-methylimidazolium
1 mM + 1 mM
68
No Improvement



Chloride + NaBr















Ionic Liquid
1-Butyl-3-
0.01
mM
66
No Improvement



methylimidazoium Chloride






Ionic Liquid
1-Butyl-3-
0.1
mM
67
No Improvement



methylimidazoium Chloride






Ionic Liquid
1-Butyl-3-
1
mM
65
No Improvement



methylimidazoium Chloride















Ionic Liquid
1-Butyl-3-
0.01 mM +
66
No Improvement



methylimidazoium Chloride +
0.01 mM





NaBr





Ionic Liquid
1-Butyl-3-
0.1 mM + 0.1 mM
63
No Improvement



methylimidazoium Chloride +






NaBr





Ionic Liquid
1-Butyl-3-
1 mM + 1 mM
67
No Improvement



methylimidazoium Chloride +






NaBr















Osmolytes
Ectoine
0.072
mM
61
No Improvement


Osmolytes
Ectoine
0.718
mM
64
No Improvement


Osmolytes
Ectoine
5
mM
45
No Improvement


Osmolytes
Firoin
0.038
mM
68
No Improvement


Osmolytes
Firoin
0.377
mM
68
No Improvement


Osmolytes
Firoin
1
mM
69
No Improvement


Substrates
Arginine
0.0058
mM
69
No Improvement


Substrates
Arginine
0.058
mM
68
No Improvement


Substrates
Arginine
0.25
mM
66
No Improvement


Flavoniods
Chrysin
0.0000254
mM
68
No Improvement


Flavoniods
Chrysin
0.0000254
mM
70
No Improvement


Flavoniods
Chrysin
0.00254
mM
68
No Improvement


Crosslinkers
Dimethyl 3,3′-
0.0000032
mM
69
No Improvement



dithioproprionimidate







dihydrochloride






Crosslinkers
Dimethyl 3,3′-
0.000032
mM
68
No Improvement



dithioproprionimidate







dihydrochloride






Crosslinkers
Dimethyl 3,3′-
0.00032
mM
68
No Improvement



dithioproprionimidate







dihydrochloride






Similar to Drabkin's Reagent
Histidine
0.0028
mM
66
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Histidine
0.0228
mM
71
No Improvement


(cyanide)







Similar to Drabkin's Reagent
Histidine
0.228
mM
68
No Improvement


(cyanide)







Substrates
Sodium Bicarbonate
0.0000895
mM
67
No Improvement


Substrates
Sodium Bicarbonate
0.000895
mM
67
No Improvement


Substrates
Sodium Bicarbonate
0.00895
mM
67
No Improvement


Sugars/Saccharides
Trehalose
1.74
mM
70
No Improvement


Sugars/Saccharides
Trehalose
10
mM
70
No Improvement


Sugars/Saccharides
Trehalose
16.76
mM
71
No Improvement











HRP Stabilizer Components
HRP Conjugate Stabilizer
  5%
79
Improvement



(PN SZ02)















Polyvalent Ions/Metal Salts
Calcium Chloride
10
mM
76
Improvement


Polyvalent Ions/Metal Salts
Magnesium Sulfate
10
mM
74
Improvement











HRP Stabilization
HRP Conjugate Stabilizer
  15%
70
Improvement


Components
(PN 85R-102)





Osmolytes
Betaine
3.5M
78
Improvement


HRP Stabilizer Components
HRP Conjugate Stabilizer
  15%
79
Improvement



(PN SZ02)





Sugars/Saccharides
Sucrose
0.24M 
75
Improvement


Sugars/Saccharides
Trehalose
0.3M
77
Improvement


Protoporphyrin
Hemin, pH 6.5
0.0003% 
90
Improvement












Protoporphyrin
Protoporphyrin IX Zinc, pH
1
uM
89
Improvement



6.5






Protoporphyrin
Protoporphyrin IX Zinc, pH
1
uM
75
Improvement



7.4






Protoporphyrin
Protoporphyrin IX
2.5
uM
117
Improvement



Chromium, pH 6.5






Protoporphyrin
Protoporphyrin IX
2.5
uM
102
Improvement



Chromium, pH 7.4






Protoporphyrin
Protoporphyrin IX Cobalt,
5
uM
109
Improvement



pH 6.5






Protoporphyrin
Protoporphyrin IX Cobalt,
5
uM
111
Improvement



pH 7.4

















APPENDIX D





Compound



Classification
Compound Combinations







Crosslinkers
4arm-PEG2K-Maleimide + Iminothiolane



hydrochloride



mPEG-Mal, 5K + Iminothiolane



hydrochloride


Sugars/Saccharides
Trehlose + Glycine



Trehalose + Glycerol



Glucose + Glycine



Glucose + Glycerol



Trehalose + Glucose



DEAE-Dextrose + Ethylene Glycol +



Calcium Chloride



Sucrose + Glucose



Sucrose + Trehalose



Sucrose + Raffinose



Raffinose + Trehalose



Raffinose + Glucose



Raffinose + Betaine



Raffinose + CaCl2



Raffinose + Ectoine



Raffinose + MgSO4



Raffinose + TMANO



Sucrose + Betaine



Sucrose + CaCl2



Sucrose + Ectoine



Sucrose + MgSO4



Sucrose + TMANO



Trehalose + Betaine



Trehalose + CaCl2



Trehalose + Ectoine



Trehalose + MgSO4



Trehalose + TMANO



Glucose + Betaine



Glucose + CaCl2



Glucose + Ectoine



Glucose + MgSO4



Glucose + TMANO



Sodium Fluoride + Glucose



Polygalacturonic Acid + CaCl2



Galacturonic Acid + CaCl2



Sodium Alginate (synthetic) + CaCl2



Sodium Alginate (Medium Viscosity) + CaCl2



Sodium Alginate (Low Viscosity) + CaCl2


Formaldehyde
Diazolidinyl Urea + Glycine + Formaldehyde


Releasers
Diazolidinyl Urea + Glycine



Diazolidinyl Urea + Zinc



Imidazolidinyl Urea + Glycine + Formaldehyde



Imidazolidinyl Urea + Glycine



Imidazolidinyl Urea + Diazolidinyl Urea


Protoporphyrins
Protoporphyrin IX + Iron Chloride



Hemin + BSA



Protoporphyrin IX Tin + BSA



Protoporphyrin IX Zinc + BSA



Protoporphyrin IX + BSA



Protoporphyrin IX + Zinc Chloride



Hemin + CaCl2



Hemin + MgSO4



Hemin + FeCl2



Hemin + FeCl3



BSA + Hemin



BSA + Hemin + CaCl2



BSA + Hemin + MgSO4



BSA + Hemin + FeCl2



BSA + Hemin + FeCl3



Hemin + Sodium Azide



Hemin + BSA + Sodium Azide


Formaldehyde +
Ammonium Chloride + Formaldehyde


Ammonium Salt
Ammonium Oxalate + Formaldehyde


Complexes
Ammonium Sulfate + Formaldehyde



Ammonium Acetate + Formaldehyde


Ionic Liquids
1-Allyl-3-methylimidazolium chloride +



Sodium Bromide



1-Butyl-3-methylimidazolium chloride +



Sodium Bromide


Polyvalent Ions/
Copper Chloride + Sucrose


Metal Salts
Iron Chloride + Sucrose



Nickel Chloride + Sucrose



Zinc Chloride + Sucrose



Cobalt Chloride + Sucrose



Magnesium Sulfate + Sucrose



Calcium Propoinate + PEG 8000



Calcium Propoinate + PEG 8000 +



Calcium Chloride



Calcium + Cobalt



Calcium + Copper



Calcium + Iron



Calcium + Magnesium



Calcium + Nickel



Calcium + Zinc



Cobalt + Copper



Cobalt + Iron



Cobalt + Magnesium



Cobalt + Nickel



Cobalt + Zinc



Copper + Magnesium



Copper + Nickel



Copper + Zinc



Iron + Magnesium



Iron + Nickel



Iron + Zinc



Magnesium + Nickel



Magnesium + Zinc



Calcium Chloride + PEG 8000



Calcium Chloride + PEG 20000



Calcium Chloride + PEG 2000



Nickel + Zinc



Iron Chloride + EDTA



Iron Chloride + IDA



Iron Chloride + GLDA


HRP Stabilizer
Surmodics HRP Stabilization Buffer +


Components
Magnesium Sulfate



Surmodics HRP Stabilization Buffer +



Calcium Chloride



Fitzgerald HRP Conjugate Buffer +



Magnesium Sulfate



Fitzgerald HRP Conjugate Buffer +



Calcium Chloride



Fitzgerald HRP Conjugate Buffer +



Cobalt Chloride



Fitzgerald HRP Conjugate Buffer +



Copper Chloride



Fitzgerald HRP Conjugate Buffer +



Nickel Chloride



Fitzgerald HRP Conjugate Buffer +



Zinc Chloride



Fitzgerald HRP Conjugate Buffer +



Iron (III) Chloride



Fitzgerald HRP Conjugate Buffer +



Aluminum Chloride



Fitzgerald HRP Conjugate Buffer +



Lithium Chloride



Fitzgerald HRP Conjugate Buffer +



Sodium Chloride








Claims
  • 1.-21. (canceled)
  • 22. A stool resuspension solution effective to stabilize hemoglobin, the solution comprising: protoporphyrin complexed with Cr3+, orprotoporphyrin complexed with Co3+,wherein the protoporphyrin complexed with Cr3+ or Co3+ is effective to stabilize hemoglobin.
  • 23. The stool resuspension solution of claim 22, wherein the concentration of the protoporphyrin complexed with Cr3+ is 1.25-5 μM.
  • 24. The stool resuspension solution of claim 22, wherein the concentration of the protoporphyrin complexed with Co3+ is 2.5-10 μM.
  • 25. The stool resuspension solution of claim 22, wherein the concentration of the protoporphyrin complexed with Cr3+ is1.5-4.5 μM.
  • 26. The stool resuspension solution of claim 22, wherein the concentration of the protoporphyrin complexed with Co3+ is 3-5 μM.
  • 27. The stool resuspension solution of claim 22, wherein the protoporphyrin is protoporphyrin IX.
  • 28. The stool resuspension solution of claim 22, wherein the solution has a pH in the range of pH 6.5 to pH 7.4.
  • 29. The stool resuspension solution of claim 23, wherein the pH of the solution is in the range of pH 6.9 to pH 7.4.
  • 30. The stool resuspension solution of claim 24, wherein the pH of the solution is in the range of pH 6.5 to pH 7.4.
  • 31. The stool resuspension solution of claim 22, wherein a stool sample is suspended in the solution.
  • 32. The stool resuspension solution of claim 22, further comprising a buffer, bovine serum albumin, polysorbate 20, sodium azide, sodium chloride, ethylenediaminetetraacetic acid, and gentamicin.
  • 33. A stool sample collection device comprising: a stool sample collection container having an open end; anda stool resuspension solution comprising protoporphyrin complexed with Cr3+ or Co3+, wherein the protoporphyrin complexed with Cr3+ or Co3+ is effective to stabilize hemoglobin.
  • 34. The stool sample collection device of claim 33, further comprising a stool sampling rod comprising a distal beveled tip for scooping and/or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device.
  • 35. The stool sample collection device of claim 34, wherein the sample collection container and sampling rod connect via a screw fit.
  • 36. The stool sample collection device of claim 33, wherein the concentration of the protoporphyrin complexed with Cr3+ is 1.25-5 μM.
  • 37. The stool sample collection device of claim 33, wherein the concentration of the protoporphyrin complexed with Co3+ is 2.5-10 μM.
  • 38. The stool sample collection device of claim 33, wherein the concentration of the protoporphyrin complexed with Cr3+ is1.5-4.5 μM and wherein the concentration of the protoporphyrin complexed with Co3+ is 3-5 μM.
  • 39. The stool sample collection device of claim 33, wherein the protoporphyrin is protoporphyrin IX.
  • 40. The stool sample collection device of claim 36, wherein the solution has a pH in the range of pH 6.5 to pH 7.4.
  • 41. The stool sample collection device of claim 37, wherein the pH of the solution is in the range of pH 6.9 to pH 7.4.
CROSS-REFERENCING

This application claims the benefit of U.S. provisional application Ser. No. 62/648,874, filed on Mar. 27, 2018, and 62/685,248, filed on Jun. 14, 2018, which applications are incorporated by reference herein.

Provisional Applications (2)
Number Date Country
62648874 Mar 2018 US
62685248 Jun 2018 US
Continuations (2)
Number Date Country
Parent 17833261 Jun 2022 US
Child 18101027 US
Parent 16355498 Mar 2019 US
Child 17833261 US