Claims
- 1. A method for the determination of a compound having a mercapto group, which comprises reacting said compound with a chromogen represented by formula (I): ##STR8## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 are independently selected from the group consisting of (II), (III) or (IV): ##STR9## wherein Z is independently selected from the group consisting of hydroxyl, optionally substituted amino, optionally substituted C.sub.1-5 alkyl, C.sub.2-5 alkenyl, optionally substituted phenyl, optionally substituted naphthyl, C.sub.2-5 acyl, halogen, nitro, sulfo, carboxyl or C.sub.1-4 alkoxy; and n is from 0 to 3, provided that at least one Z is hydroxyl or optionally substituted amino, wherein the optional substituents are selected from the group consisting of C.sub.1-5 alkyl, C.sub.2-5 alkenyl, phenyl, naphthyl, C.sub.1-4 alkoxy, hydroxyl, carboxyl, sulfo, sulfonyl, halogen, amino, C.sub.1-4 alkoxycarbonyl, C.sub.1-5 aminoalkyl, C.sub.2-5 acyl and nitro in the presence of peroxidase or thiol oxide reductase and qualitatively or quantitatively determining whether or not pigment is thus formed.
- 2. A method according to claim 1, wherein R.sub.1 and R.sub.2 are formula (II).
- 3. A method according to claim 1, wherein R.sub.1 and R.sub.2 are formula (II) and R.sub.3 is selected from group consisting of (II), (III) and (IV).
- 4. A method according to claim 1, wherein said mercapto compound is provided by a reaction system which produces a mercapto compound.
- 5. A method according to claim 4, wherein said reaction system comprises the reaction of acetyl CoA with a second reactant in the presence of an enzyme.
- 6. A method according to claim 5, wherein said second reactant is a member selected from the group consisting of L-aspartate, chloramphenicol, gentamicin C.sub.la, gentamicin C.sub.l, choline and an amino acid.
- 7. A method according to claim 1, wherein said determination of pigment is carried out by photometric measurement.
- 8. A method according to claim 7, wherein said measurement is carried out by measuring the absorption of the reaction solution.
- 9. A method according to claim 8, wherein said absorption is measured using rays having wavelengths in the visible ray region.
- 10. A method according to claim 7, wherein said measurement is carried out by measuring the rate of absorption change of the reaction solution.
- 11. A method according to claim 1, wherein said reaction is carried out in a buffer.
- 12. A method according to claim 1, using peroxidase and (I).
- 13. A method according to claim 1, wherein said mercapto compound is a member selected from the group consisting of L-cysteine, glutathione, CoA, methanethiol, dihydrolipoamide and thiocholine.
Priority Claims (1)
Number |
Date |
Country |
Kind |
59-74714 |
Apr 1984 |
JPX |
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Parent Case Info
This application is a continuation of application Ser. No. 721,951 filed Apr. 11, 1985 now abandoned.
US Referenced Citations (6)
Foreign Referenced Citations (6)
Number |
Date |
Country |
0057661 |
Aug 1982 |
EPX |
0100217 |
Feb 1984 |
EPX |
153872 |
Sep 1985 |
EPX |
162685 |
Nov 1985 |
EPX |
2944498 |
May 1980 |
DEX |
0008797 |
Jan 1982 |
JPX |
Non-Patent Literature Citations (1)
Entry |
Chemical Abstracts, vol. 90, No. 23 (1979), 182296q. |
Continuations (1)
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Number |
Date |
Country |
Parent |
721951 |
Apr 1985 |
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