Claims
- 1. A method for producing Factor VII comprising (a) cultivation of a mammalian cell line comprising a DNA sequence encoding yeast KEX2 endopratease and a DNA sequence encoding Factor VII (FVII) in a suitable culture medium, under conditions in which both said KEX2 endoprotease and said FVII are expressed; and (b) isolation of Factor VII from the medium.
- 2. The method of claim 1, wherein the culture medium is a serum free medium.
- 3. The method of claim 1 wherein the mammalian cell line is a CHO cell line.
- 4. The method of claim 1 wherein the mammalian cell line is a BHK cell line.
- 5. The method of claim 1 wherein the mammalian cell line is a HEK 293 cell line.
- 6. A method for producing Factor VII comprising (a) cultivation of a mammalian cell line comprising a DNA sequence encoding (i) a variant of yeast KEX2 endoprotease, wherein said variant is selected from the group consisting of KEX2 1-614 and KEX2 1-675, and (ii) a DNA sequence encoding Factor VII (FVII) in a suitable culture medium, under conditions in which both said KEX2 endopratease and said FVII are expressed; and (b) isolation of Factor VII from the medium.
- 7. The method of claim 6, wherein the mammalian cell is selected from the group consisting of CHO, BHK, and HEK293 cells.
- 8. The method of claim 6, wherein the culture medium is a serum-free medium.
Priority Claims (2)
Number |
Date |
Country |
Kind |
01436/98 |
Nov 1998 |
DK |
|
01439/98 |
Nov 1998 |
DK |
|
CROSS-REFERENCES TO RELATED APPLICATIONS
The present application is a continuation of PCT/DK99/00607 filed Nov. 5, 1999 and claims priority under 35 U.S.C. 119 of U.S. provisional application no. 60/108,065 filed Nov. 12, 1998 and Danish application nos. PA 1998 01439 filed Nov. 9, 1998 and PA 1998 01436 filed Nov. 6, 1998, the contents of which are fully incorporated herein by reference.
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Non-Patent Literature Citations (2)
Entry |
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Provisional Applications (1)
|
Number |
Date |
Country |
|
60/108065 |
Nov 1998 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
PCT/DK99/00607 |
Nov 1999 |
US |
Child |
09/558027 |
|
US |