Claims
- 1. A method of preparing an antigen comprising:
i) contacting a pyrrole-containing biological compound with one of:
a) an optionally labelled derivatizing agent (bound to, or able to bind with a solid support) wherein the derivatizing agent forms a reaction product with the biological compound, followed by exposure to a detectable molecule which forms a complex with the reaction product; or b) an optionally labelled derivatizing agent not bound to a solid support, wherein the derivatizing agent forms a reaction product with the biological compound, followed by exposure to a binding agent specific to the biological compound in the reaction product, said binding agent being bound to a solid support; or; c) a binding agent bound to a solid support, said binding agent being specific to the biological compound and forming a complex therewith, followed by exposure to an optionally labelled, derivatizing agent which forms a reaction product with the biological compound moiety of said complex; ii) eluting the biological compound from the solid support.
- 2. A method according to claim 1 wherein the derivatizing agent is of the following structure in bound form:
- 3. A method according to claim 1 wherein the derivatizing agent is of the following structure in bound form:
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is a divisional of co-pending U.S. application Ser. No. 09/970,328, filed Oct. 2, 2001, which is a continuation-in-part of U.S. application Ser. No. 09/679,141, filed Oct. 3, 2000 (now abandoned), the disclosures of which are incorporated herein by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09970328 |
Oct 2001 |
US |
Child |
10671979 |
Sep 2003 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09679141 |
Oct 2000 |
US |
Child |
09970328 |
Oct 2001 |
US |