Claims
- 1. A method of controlling insect infestation of plants comprising providing a 3-hydroxysteroid oxidase and a CrylA Bacillus thuringiensis protein in an insecticidally effective amount for ingestion by the insect where the insect is identified as a lepidopteran.
- 2. The method of claim 1 wherein the insect is in a larval stage.
- 3. The method of claim 1 wherein the CryIA is CryIA(c).
- 4. The method of claim 1 wherein the CryIA is CryIA(b).
- 5. The method of claim 3 wherein the insect is tobacco budworm or beet armyworm.
- 6. The method of claim 4 wherein the insect is European corn borer, black cutworm or fall armyworm.
- 7. The method of claim 4 wherein the insect is cotton bollworm or corn earworm.
- 8. The method of claim 1 wherein the insecticidally effective amount is between about 8 and about 20 ppm.
- 9. A recombinant expression vector comprising nucleic acid sequences encoding a CrylA protein and a 3-hydroxysteroid oxidase, each sequence operatively linked to a promoter to express separately or in tandem said nucleic acid sequences to produce said CrylA protein and said 3-hydroxysteroid in a plant cell.
- 10. The vector of claim 9 wherein the plant cell is identified as a corn cell.
- 11. A method of producing a genetically transformed plant which produces an insecticidally effective amount of a CrylA protein and a 3-hydroxysteroid oxidase, comprising inserting into the genome of a plant cell a recombinant vector comprising:
- a) a sequence encoding 3-hydroxysteroid oxidase;
- b) a sequence encoding a CrylA protein; and,
- c) at least one promoter that functions in a plant cell which promoter is heterologous with respect to said coding sequences operatively linked to effect expression of said coding sequences to produce the CrylA protein and the 3-hydroxysteroid oxidase in an insecticidally effective amount in a genetically transformed plant obtained from the plant cell.
- 12. The method of claim 11 wherein the plant is a cotton plant.
- 13. The method of claim 11 wherein the plant is a corn plant.
- 14. A plant produced by the method of claim 11.
- 15. A seed produced by the plant of claim 11.
- 16. The seed of claim 15 which is a cotton seed or a corn seed.
- 17. The method of claim 11 wherein the CrylA protein is identified as a CryIA(b) protein.
- 18. The method of claim 11 wherein the CrylA protein is identified as a CryIA(c) protein.
Parent Case Info
The application is a continuation-in-part of U.S. Ser. No. 08/475,694 filed Jun. 7, 1995, now U.S. Pat. No. 5,558,862 issued Sep. 24, 1996, which is a continuation-in-part of U.S. Ser. No. 08/393,785 filed Feb. 24, 1995, now U.S. Pat. No. 5,554,369 issued Sep. 10, 1996, which is a Division of U.S. Ser. No. 08/083,948 filed Jun. 28, 1993, now U.S. Pat. No. 5,518,908 issued May 21, 1996, which is a continuation-in-part of U.S. Ser. No. 08/937,195 filed Sep. 4, 1992, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07/762,682 filed Sep. 23, 1991, now abandoned.
US Referenced Citations (4)
Number |
Name |
Date |
Kind |
5518908 |
Corbin et al. |
May 1996 |
|
5530197 |
Peferoen |
Jun 1996 |
|
5554369 |
Corbin et al. |
Sep 1996 |
|
5558862 |
Corbin et al. |
Sep 1996 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0 385 962 |
Sep 1990 |
EPX |
Divisions (1)
|
Number |
Date |
Country |
Parent |
83948 |
Jun 1993 |
|
Continuation in Parts (4)
|
Number |
Date |
Country |
Parent |
475694 |
Jun 1995 |
|
Parent |
393785 |
Feb 1995 |
|
Parent |
937195 |
Sep 1992 |
|
Parent |
762682 |
Sep 1991 |
|