Claims
- 1. A recombinant, double-stranded DNA molecule comprising in operative sequence:
- a) a promoter which functions in plant cells to cause the production of an RNA sequence;
- b) a structural coding sequence that encodes for production of Aspergillus glucose oxidase; and
- c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
- 2. The DNA molecule of claim 1 wherein said structural DNA sequence is SEQ ID NO:1.
- 3. The DNA molecule of claim 1 wherein said promoter is selected from FMV35S and CaMV35S promoters.
- 4. The DNA molecule of claim 1 wherein said promoter is induced by a pathogenic infection.
- 5. A method of producing genetically transformed, disease resistant plants, comprising the steps of:
- a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising
- (i) a promoter which functions in plant cells to cause the production of an RNA sequence;
- (ii) a structural coding sequence that causes the production of Aspergillus glucose oxidase;
- (iii) a 3' non-translated region which functions in said plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence;
- b) obtaining transformed plant cells; and
- c) regenerating from the transformed plant cells genetically transformed plants which express Aspergillus glucose oxidase in an amount effective to reduce damage due to infection by a bacterial or fungal pathogen.
- 6. The method of claim 5 wherein said structural coding sequence is SEQ ID NO:1.
- 7. The method of claim 5 wherein said promoter is selected from FMV35S and CaMV35S promoters.
- 8. The method of claim 5 wherein said promoter is induced by pathogen infection.
- 9. A genetically transformed, disease resistant plant comprising a recombinant, double-stranded DNA molecule comprising in operative sequence:
- a) a promoter which functions in plant cells to cause the production of an RNA sequence;
- b) a structural coding sequence that encodes for production of Aspergillus glucose oxidase; and
- c) a 3' non-translated region which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA sequence.
- 10. The plant of claim 9 wherein said promoter is selected from FMV35S and CaMV35S promoters.
- 11. The plant of claim 9 wherein said promoter is induced by pathogen infection.
- 12. The plant of claim 9 wherein said structural coding sequence is SEQ ID NO:1.
- 13. The plant of claim 12 which is a potato plant.
Parent Case Info
This application is a continuation-in-part of U.S. Ser. No. 161,041, filed Nov. 24, 1993, now abandoned.
US Referenced Citations (5)
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO8912675 |
Dec 1989 |
WOX |
Non-Patent Literature Citations (4)
Entry |
Kim et al., Identification of a Metabolite Produced by Talaromyces flavus as Glucose Oxidase and its Role in the Biocontrol of Verticillium dahliae. Phytopathology 78:488-492, 1988. |
Kim et al., Production, purification, and properties of glucose oxidase from the biocontrol fungus Talaromyces flavus. Can. J. Microbiol. 36:199-205 1990. |
Koster-Topfer et al., A Class II Patatin Promoter Is under Developmental Control in Both Transgenic Potato and Tobacco Plants. Mol. Gen. Genet 219:390-396, 1989. |
Kriechbaum et al., Cloning and DNA Sequence Analysis of the Glucose Oxidase Gene from Aspergillus niger NRRL-3. FEBS Lett. 225(1):63-66, 1989. |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
161041 |
Nov 1993 |
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