Claims
- 1. In combination, an analytical apparatus for the quantitative determination of a material selected from the group of polycyclic aromatic hydrocarbons, dansylamino acids and vitamins in a mixture comprising:
- (a) means defining and containing a supply of said materials to be separated;
- (b) a liquid column chromatograph for separating said material to form a column eluate containing the separated said material;
- (c) said material separating means in said chromatographic column;
- (d) a first mixing zone;
- (e) means defining and containing a supply of oxalic acid derivative reagent;
- (f) means for passing a solution of an oxalic acid derivative to said first mixing zone at a controlled rate;
- (g) means defining and containing a supply of hydrogen peroxide reagent;
- (h) means for passing a solution of hydrogen peroxide to said first mixing zone at a controlled rate;
- (i) means for simultaneously introducing the separated solution, oxalic acid derivative reagent and hydrogen peroxide into said first mixing means;
- (j) means in said first mixing zone to mix said column eluate, said oxalic acid derivative, and said hydrogen peroxide to form a first mixture;
- (k) a coiled mixing zone through which said first mixture is continuously passed to form a second mixture, whereby chemiluminescence occurs; and
- (l) a light-free detecting section comprising a flow cell and a light receptive detection means for perceiving and multiplying the light produced by the chemiluminescence reaction.
- 2. The apparatus of claim 1, wherein said means for defining and containing a supply of oxalic acid derivative reagent contains oxalic acid derivatives selected from the group consisting of oxalic acid esters, oxalic acid chlorides, oxalic acid anhydrides and oxalic acid amides.
Priority Claims (1)
Number |
Date |
Country |
Kind |
55-39922 |
Apr 1979 |
JPX |
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Parent Case Info
This application is a continuation of application Ser. No. 292,487, filed Aug. 31, 1981 which is a division of application Ser. No. 80,294, filed Oct. 1, 1979, now abandoned.
US Referenced Citations (8)
Divisions (1)
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Number |
Date |
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Parent |
80294 |
Oct 1979 |
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Continuations (1)
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Number |
Date |
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Parent |
292487 |
Aug 1981 |
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