Claims
- 1. A method of detecting variant nucleic acids whose nucleotide sequences differ from one another in at least one position X, comprising the steps of:
- a) in a single reaction vessel combining a set of oligonucleotides, whose nucleotide sequences differ from one another in a position which corresponds to position X and in at least one additional position Y, with the nucleic acids to be detected under hybridization conditions,
- b) extending any oligonucleotides which hybridize to the nucleic acids to be detected to form extension products, wherein said nucleic acids are used as templates,
- c) denaturing complexes of said variant nucleic acids and said extension products,
- d) combining said extension products with a set of detection-oligonucleotides under hybridization conditions,
- i) wherein a portion of each detection-oligonucleotide is complementary to a portion of an oligonucleotide from said set of oligonucleotides according to step a) and said complementary portion comprises the positions X and Y of said oligonucleotide of step a), and
- ii) wherein a portion of each detection oligonucleotide is complementary to a part of a sequence which was added to said oligonucleotide from the set of oligonucleotides in extending step b), and
- e) detecting said detection-oligonucleotides which hybridize to said extension products.
- 2. The method according to claim 1, wherein the extension products formed in step (b) are bound to a solid phase before said detecting step (e).
- 3. The method according to claim 1, wherein at least one oligonucleotide of said set of detection-oligonucleotides contain at least one immobilizable or one detectable label.
Priority Claims (1)
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41 29 653.2 |
Sep 1991 |
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Parent Case Info
This application is a division of application Ser. No. 08/205,777, filed Mar. 3, 1994, U.S. Pat. No. 5,605,794, which is a continuation of application Ser. No. 07/939,479, filed Sep. 4, 1992, abandoned.
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Divisions (1)
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Parent |
205777 |
Mar 1994 |
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Continuations (1)
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939479 |
Sep 1992 |
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